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Citations for Millipore Sigma :
451 - 500 of 3648 citations for IL 2RG Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The His-tag was removed by O/N incubation with TEV protease (Millipore Sigma) at 4 °C followed by negative INAC ...
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bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were detected using monoclonal anti-polyHis-HRP conjugate (Sigma-Aldrich, CAD). Phosphorylated mitogen-activated protein kinases (MAPK ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
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bioRxiv - Immunology 2022Quote: ... The lysate was mixed gently with 50% Ni-NTA His-bind slurry (EMD Millipore) at 4:1 ratio on a shaking platform for 60 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Cell Biology 2019Quote: ... input was taken and 20 µl HIS-Select® HF Nickel Affinity Gel (Sigma) pre-equilibrated with Pull-Down Buffer was added to each sample and incubated on an overhead rotator for 1h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was incubated with 6 ml HIS-Select Nickel Affinity Gel (Sigma), gently agitated at 4°C for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by immunoblotting with primary mouse anti-His (Sigma□Aldrich, Taiwan) or anti-GST primary antibodies (Sigma□Aldrich ...
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bioRxiv - Immunology 2020Quote: ... BM cells were cultured in growth media supplemented with 10% HI FBS (Sigma-Aldrich), 1% Pen/Strep (Lonza) ...
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bioRxiv - Immunology 2020Quote: ... and loaded onto a cOmplete™ His-Tag Purification Resin gravity column (Sigma Millipore). Resin was first washed with detergent-containing buffer (25 mM Tris + 500 mM NaCl + 0.5 % N-Dodecyl-β-D-maltoside ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tag was removed by treating purified nanobody Nb_0 with carboxypeptidase A (Sigma) and B (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... Expression of pAgos was verified by Western blot with antibodies against His-tag (Sigma) after cell growth for 2 hours at 30 °C ...
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bioRxiv - Bioengineering 2022Quote: ... and mVenus-SpyCatcher-6×His were prepared using Gibson assembly with pET-19b (Novagen) as the plasmid vector ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mpp75Aa1.1 or Mpp75Aa1.1-His was mixed with high purity pepsin (Sigma, St. Louis, MO) in 2 mg/ml NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS-hi; Sigma, Cat. no. F7524), 1% antibiotic-antimycotic (AA ...
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bioRxiv - Biochemistry 2024Quote: ... and dilutions were as follows (in parenthesis): His (mouse, Sigma cat# H1029; 1:2,500); β-actin (rabbit ...
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bioRxiv - Cell Biology 2020Quote: ... and containing 125 pg/mL recombinant IL-15 (Sigma-Aldrich) to aid uNK cell maturation ...
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bioRxiv - Neuroscience 2020Quote: ... IL-1β (SIGMA, #SRP8033, i.p., 3 μg/kg body weight).
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bioRxiv - Neuroscience 2020Quote: E-4031 (Alomone-Labs, IL) and TEA (Sigma-Aldrich, USA) were dissolved in 0.9% saline ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich), and 0.5 μg/ml anti-CD180 (RP/14 ...
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bioRxiv - Bioengineering 2022Quote: ... 0.1mM ascorbic acid and 50 pg/mL IL-1β (Sigma).
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/ml mouse recombinant IL-4 (Sigma-Aldrich) to allow B cell activation and class switch recombination to IgG1 ...
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bioRxiv - Immunology 2021Quote: HEK293 and transfected derivatives were left unstimulated or stimulated for 24h with 10 ng/mL lipopolysaccharide (LPS) from Salmonella (Sigma), 10 ug/mL isolated S protein ...
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bioRxiv - Neuroscience 2020Quote: ... the HEK293 and primary neuronal cells were plated on the 12mm #1 coverslips coated with poly-D-lysine (Sigma-Aldrich). For the 96-well plate screening assays ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells were seeded at a low density on 0.01% (w/v) poly-L-lysine (Sigma, St Louis, MO, USA) coated coverslips and were transfected the subsequent day using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The exogenous expression of ACE2 and TMPRSS2 in the HEK293-C34 cells was induced by addition of doxycycline hydrochloride (1 μg/ml) (Sigma). TMPRSS2-expressing Vero E6 (VeroE6/TMPRSS2 ...
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bioRxiv - Molecular Biology 2020Quote: pCDNA3 vectors expressing HA-tagged full size LARP6 or La-domain were transfected into HEK293 cells and 24h after transfection the cells were fixed and incubated with anti-HA primary antibody (Sigma), followed by incubation with fluorescent secondary antibody ...
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bioRxiv - Immunology 2019Quote: ... Vectors were transiently transfected in HEK293-6E cells and antibodies were purified from supernatant using Robocolumn Eshmuno A (EMD Millipore) or Monofinity A Resin prepacked columns (Genscript) ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected with plasmids encoding different ACE2 orthologs (Table S1) by polyetherimide (PEI) (Sigma, St Louis, MO, USA). After 40 hrs incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... thereafter named HEK293) were cultured under 5% CO2 at 37°C in low-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Sigma–Aldrich, D6046) supplemented with 10% foetal bovine serum (FBS-Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... Then pSIREN-RetroQ-Sirt7 and the negative control vector (pSIREN-RetroQ) were transfected into HEK293 cells using PEI transfection reagent (Sigma). The pSIREN-RetroQ-Sirt7 and the negative control vector (pSIREN-RetroQ ...
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bioRxiv - Biochemistry 2023Quote: HEK293 Flp-In T-Rex cells were cultured in DMEM high glucose medium supplemented with 10% FBS (Sigma-Aldrich F0804), 2mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: PFKFB3 depletion was carried out in HEK293 cells using siRNAs (80 nM; transfected twice on consecutive days) from SIGMA (SASI_Hs01_00065120). The cells were treated with control or specific siRNA and lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: 2×10^5 Vero E6 cells or HEK293-ACE-2 were cultured in 24-well plates in Dulbecco’s Modified Eagle medium (DMEM, Sigma D5796) containing 10% of fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... and GIGYF2-KO TLR3-HEK293 cells were either mock-treated or transfected with 1 μg/mL Poly(I:C) (Sigma, P1530) using Lipofectamine2000 ...
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bioRxiv - Cell Biology 2024Quote: Doxycycline-inducible stable Flp-In T-REx HEK293 cell lines expressing mitochondrial-relocated golgin-BirA* fusion proteins were induced with 1 μg/ml doxycycline (Sigma) in culture medium once they reached approximately 80% confluency ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Microbiology 2020Quote: ... Human serum-supplemented medium was made through addition of complete human serum (Sigma Aldrich) into CDM to a final concentration of 5% (v/v) ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in an equal volume of human serum from human male AB plasma (Sigma) and incubated for 16 h at 37 °C ...
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bioRxiv - Biophysics 2024Quote: ... supplemented with 10 % heat-inactivated human serum from human male AB plasma (Sigma-Aldrich), 2.5 μM zoledronic acid (Sigma-Aldrich ...
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bioRxiv - Genetics 2019Quote: ... from HEK293 cells stably expressing p3XFLAG-ADCK4 or -BAP were incubated with 80 μL of FLAG M2 agarose beads (Sigma-Aldrich) for 48 h at 4°C in an orbital shaker ...
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Cell Biology 2021Quote: ... GBM#U3013 cells were infected with lentiviral particles generated by transfecting HEK293 cells with pLKO.1-puro plasmids from the Mission shRNA library (Sigma-Aldrich). Briefly ...
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bioRxiv - Immunology 2021Quote: ... HEK293 and HEK293T cells (gifts from Caetano Reis e Sousa, The Francis Crick Institute, UK) were maintained in DMEM (Sigma-Aldrich) containing 4.5 g/L glucose ...
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bioRxiv - Immunology 2022Quote: Luciferase-expressing HeLa or HEK293 cells were seeded into a U-bottomed 96-well plate plus D-Luciferin (75 μg/ml; Sigma-Aldrich) for cytotoxicity assessment ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were cultured in serum-free and phenol red-free media containing 4.5 g/L glucose (Sigma-Aldrich, Dorset, UK), 1% penicillin/streptomycin ...
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bioRxiv - Biochemistry 2019Quote: Cell lysate from HEK293 transfected with FLAG-Podocin (WT, R45A, R50 and R54A) was immobilized on anti-FLAG beads (Sigma-Aldrich). The beads were washed with IP buffer (1%–2% Triton-X ...