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Citations for Millipore Sigma :
151 - 200 of 3146 citations for IL 1RA Human HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 and HeLa cells were grown in Dulbecco’s modified Eagle’s medium (DMEM)-GlutaMAX™ -I (Sigma-Aldrich) supplemented with 10% fetal bovine serum (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... HEK293 cells (2 x 104 cells per well) were transiently transfected using X-tremeGENE™ 9 (Sigma) with plasmids encoding eYFP tagged PAR2 (PAR2-eYFP ...
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bioRxiv - Biophysics 2021Quote: ... and HEK293 control cells were separated by SDS-PAGE using TruPAGE precast gels (4-12%) (Sigma-Aldrich) or 8% Laemmli SDS-gels ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were co-transfected with plasmids with AKT-PH::GFP and shRNA against KIAA1109 (Sigma-Aldrich, TRCN0000263343 with 73% knockdown efficiency) ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells from ATCC (ATCC# CRL-1573) cells were cultured in DMEM cell culture media (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... IL-1Rα and IL-10) were analyzed by multiplex assays using custom multiplex cytokine plates (EMD Millipore, Billerica, MA, USA). Data were collected on a MAGPIX instrument (Luminex ...
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bioRxiv - Immunology 2024Quote: ... or alternatively plus IL-4 (4ng/ml) or IL-4 and lipopolysaccharide (LPS) (10μg/ml) (Sigma-Aldrich, St. Louis, MO) for 2.5 days.
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bioRxiv - Molecular Biology 2021Quote: ... and 80 ng/ml IL-2 (Sigma-Aldrich) for their activation ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 100 U/ml IL-2 (Sigma).
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bioRxiv - Genomics 2019Quote: ... along with IL-2 (30 U/ml; Sigma). When indicated ...
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bioRxiv - Neuroscience 2023Quote: ... anti-mouse IL-6 ELISA kit (Sigma, RAB0309), anti-mouse G-CSF ELISA kit (MCS00 ...
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bioRxiv - Neuroscience 2024Quote: ... Cytokine (TNF-α, IL-1α, C1q-Sigma-Aldrich) stock solutions (1000X ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... or human liver microsomes (HLMs) (male human pooled, Sigma-Aldrich), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Human haemoglobin (Sigma) was used for creation of standard curve.
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bioRxiv - Microbiology 2021Quote: ... human insulin (Sigma), 10’000 units/ml of penicillin and 10’000 µg/ml streptomycin (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... human HDL (Millipore), human LDLs (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... human LDLs (Millipore) or an in vitro reconstituted NS1-HDL mix were analyzed by size exclusion chromatography on a Superdex 200 10/300 column (GE healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... Human hemoglobin (Sigma) was dissolved in PBS to 10 mg/mL or 1 mg/mL ...
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bioRxiv - Microbiology 2023Quote: ... Human DPP4 (Sigma) was used as a control in PBS at 50 ng/μL (0.585 μM) ...
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bioRxiv - Neuroscience 2021Quote: The transfected HEK293 cells were seeded on glass coverslips (Matsunami) coated with poly-L-lysine solution (Sigma-Aldrich) then incubated for 4–10 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Neuro-2a (N2a) and HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) high glucose (Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2019Quote: ... HEK293 cell viability after iOn plasmids transfection was assessed by dye exclusion with Trypan blue solution (0.4%, Sigma). FP expression was either assayed by flow cytometry ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293T and HEK293-FT cells were maintained in DMEM supplemented with 10% FBS,1 mM sodium pyruvate (Sigma), 2 mM L-glutamine (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: HEK293-FLP cells stably expressing UBC:DOR-APEX2 were plated onto poly-L-lysine (Sigma Aldrich, Cat # P8920-100ML) coated coverslips in wells of a 24-well plates ...
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bioRxiv - Microbiology 2023Quote: HeLa cells and HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% foetal bovine serum (Sigma) at 37°C in 5% (V/V ...
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bioRxiv - Immunology 2022Quote: ... penicillin-streptomycin (Hyclone, Chicago, IL), 10% heat-inactivated fetal bovine serum (Hyclone, Chicago, IL) and beta-mercaptoethanol (Sigma-Aldrich, St. Louis, MO). Cell lines have been recently authenticated and were periodically tested for mycoplasma species and were confirmed negative.
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bioRxiv - Bioengineering 2019Quote: ... 100 µg/ml human recombinant Delta-1 or human IgG (Sigma) was added ...
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bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng/mL interleukin-4 (IL-4, Sigma-Aldrich) and 0.5 μg/mL anti-CD180 (BD PharMingen) ...
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bioRxiv - Microbiology 2022Quote: ... recombinant IL-2 (100 U/mL; Sigma Millipore #11011456001), and anti-CD28 + anti-CD49d (1.5ug/ml ...
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bioRxiv - Microbiology 2022Quote: ... recombinant IL-2 (100 U/mL; Sigma Millipore #11011456001), and anti-CD28 + anti-CD49d (1.5ug/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with interleukin-6 (IL-6, Sigma) for 24 ...
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bioRxiv - Cancer Biology 2023Quote: ... Interleukin-3 (IL-3, Cat# SRP4135-10UG, Sigma, USA), Alexa Fluor 488 EGF complex (Cat# E13345 ...
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bioRxiv - Cell Biology 2023Quote: ... Media containing Il-1α (3ng/ml; Sigma-Aldrich, I13901), TNFα (30ng/ml ...
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bioRxiv - Physiology 2023Quote: ... and 3 ng/mL IL-1β (Sigma-Aldrich, #H6291), and the incubation was extended for another 24 to 96 hours ...
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bioRxiv - Biophysics 2021Quote: The cAMP synthesis of heterologously expressed sAC in HEK293 lysates was induced by applying di-sodium-ATP (Sigma Aldrich) and MgCl2 or MnCl2 in isotonic HEPES buffer ...
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bioRxiv - Biochemistry 2020Quote: ... control and SENP3 over-expressed HEK293 cells were stimulated by poly (I:C) for 6h and lysed in NETN buffer supplemented with 10mM PMSF (Sigma). The whole cell lysates were immunoprecipitated with 10 μl Anti-Flag (M2)-agarose (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells transfected and grown overnight prior to incubation with 5μg/ml cycloheximide (CHX; Sigma-Aldrich, St. Louis, MO) in the presence and absence of 15μM CQ (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... murine fibroblast (3T3, laboratory stocks) and HEK293 (laboratory stocks) cell lines were maintained in Dulbecco’s minimal essential medium (DMEM, Sigma) supplemented with 10% (v/v ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) and K562 cells in RPMI-1640 medium (Sigma-Aldrich). All media had high glucose concentration and were supplemented with 10% fetal bovine serum and 2 mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
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bioRxiv - Neuroscience 2023Quote: ... were produced in HEK293 cells by mixing lentiviral vector and packaging vectors using branched polyethyleneimine (bPEI) (MW 25Kda, Sigma) as the transfection reagent ...
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bioRxiv - Immunology 2024Quote: ... IL-31 and IL-33 cytokines with multiplexed-ELISA assay with Luminex 200 Multiplex Bio-Plex 200 System (EMD Millipore, Billerica, MA, USA) using a Milliplex Map kit (EMD Millipore).
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bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... human Akt1 (Sigma-Aldrich) or 0.3 μg active ...
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bioRxiv - Biochemistry 2022Quote: ... Purified human ApoA1 (Sigma) or purified ApoA1-ApoM protein was resuspended in PBS (10:1 mol/mol ...
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bioRxiv - Bioengineering 2019Quote: Human holo Tf (Sigma) was conjugated to AF700 or AF750 (Life Technologies ...