Labshake search
Citations for Millipore Sigma :
251 - 300 of 4007 citations for IL 18 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... All antibodies were incubated separately at a concentration of 10 μg/ml in tubes together with 330 ng/ml HIS-tagged CD40 recombinant protein and 4 μg/ml peroxidase-coupled anti-HIS detection antibody (Sigma-Aldrich) for 60 minutes in ELISA buffer (PBS ...
-
bioRxiv - Immunology 2021Quote: ... 10ng/ml IL-4 (Sigma), 40nM LY2090314 (Sigma).
-
bioRxiv - Immunology 2021Quote: ... + 0.4ng/ml IL-7 (Sigma)+40µl/ml IL-7 (obtained from IL-7 producing J558L cells ...
-
bioRxiv - Immunology 2020Quote: ... IL-2 (I2644, Sigma-Aldrich); αCD3 (40-0038 ...
-
bioRxiv - Molecular Biology 2021Quote: ... and IL-6 (Sigma-Aldrich) treatments were performed at a concentration of 50 units/mL and 50 ng/mL in cDMEM ...
-
bioRxiv - Genomics 2019Quote: ... Cells were treated 18 hours later with etoposide (Sigma E1383) at 0.15 μM ...
-
bioRxiv - Genomics 2021Quote: ... containing 18% w/v Polyethylene glycol MW=8000 (Sigma-Aldrich) were used ...
-
bioRxiv - Systems Biology 2020Quote: ... and GSH (Sigma-Aldrich; Cat#S4251; CAS: 70-18-8) standards at concentrations of 2e-2 ...
-
bioRxiv - Immunology 2023Quote: ... an 18 μl droplet of 0.01% PLL solution (Sigma-Aldrich) was carefully added to each coverslip ...
-
bioRxiv - Neuroscience 2023Quote: ... The samples were then desalted with C-18 ZipTips (Millipore), dehydrated in a SpeedVac for 18 mins and reconstituted in 0.1% formic acid before undergoing liquid-chromatography electrospray ionization mass spectrometry (LC-ESI MS/MS ...
-
bioRxiv - Biophysics 2023Quote: ... N′-tetramethylethylenediamine (TEMED) (Sigma-Aldrich T9281, CAS 110-18-9) as the catalyst ...
-
bioRxiv - Bioengineering 2023Quote: ... [18] and/or supplemented with 5 mM Phe (Sigma-Aldrich) and/or 5 mM Glu (Sigma-Aldrich) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... ‘Ultrapure’ reverse osmosis water (18 MΩ.cm, Millipore, low in mercury) was used to prepare all solutions ...
-
bioRxiv - Immunology 2024Quote: ... and 18% (vol/vol) Opti-prep (Sigma-Aldrich, #D1556-250ML) in HBSS buffer without calcium or magnesium (Gibco ...
-
bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
-
bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
-
bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
-
bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
-
bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
-
bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
-
bioRxiv - Biochemistry 2021Quote: ... recombinant His-Akt1 was from EMD Millipore (#14-279); recombinant GST-mTORf (containing a 30 fragment of mTOR encoding amino acids 2144-2175 ...
-
bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
-
bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
-
bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were seeded on to #0 coverslips coated with poly-L-lysine (Sigma) in a 24-well culture dish and kept in an incubator at 37°C under air with 5% CO2 ...
-
bioRxiv - Biochemistry 2019Quote: HEK293 cells were grown in DMEM (SLRI media facility) plus 10% FBS (Sigma, F1051). For analysis of SMN chemical antagonists ...
-
bioRxiv - Molecular Biology 2020Quote: ... Constructs were transfected into HEK293 cells with the X-tremeGENE 9 reagent (Sigma-Aldrich) in optiMEM solution for 4 hours ...
-
bioRxiv - Molecular Biology 2020Quote: ... Constructs were transfected into HEK293 cells with the X-tremeGENE 9 reagent (Sigma-Aldrich) and submitted to selection with G418 (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2019Quote: ... Blocking solution for HEK293 and iPS cells respectively contained 10% normal goat serum (Sigma) or fetal bovine serum (Eurobio ...
-
bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were grown in EX-CELL® ACF CHO Medium (Sigma-Aldrich, C5467) supplemented with 100 IU/ml penicillin and 100 μg/ml streptomycin at 37°C with 95 % humidity and 5 % CO2 ...
-
bioRxiv - Cell Biology 2022Quote: HEK293 (ATCC, CRL-1573) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, D5796) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Molecular Biology 2022Quote: HEK293 cells were maintained in DMEM supplemented with 10% fetal bovine serum (Sigma, #F0804) and 1% Penicillin/Streptoymycin (HyClone ...
-
bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: HEK293 cells stably expressing FLAG-GPR65 were plated to poly-d-lysine (Sigma Aldrich) coated 12 mm glass coverslips (Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... HEK293 48-well cell cultures were supplemented with 100 µM guanosine (Sigma Aldrich #G6264). DMSO and 100 µM adenosine (Sigma Aldrich #A9251 ...
-
bioRxiv - Cell Biology 2024Quote: ... HEK293-TRPM2tet cells were treated with tetracycline hydrochloride (1 µg/ml; T7660, Sigma-Aldrich) for 48 hours ...
-
bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
-
bioRxiv - Cell Biology 2022Quote: Serum IL-6 levels were quantified using a commercial mouse IL-6 ELISA kit (RAB0308, Sigma) according to the manufacturer’s instructions and analyzed using spectra-iMAX (Molecular Devices).
-
bioRxiv - Cell Biology 2022Quote: ... and 18 mm coverslips were activated using 3-aminopropyltrimethoxysilane (Sigma Aldrich) for 5 min and then for 30 min with 0.5% glutaraldehyde (Electron Microscopy Sciences ...
-
bioRxiv - Cell Biology 2022Quote: ... and 18 mm coverslips were activated using 3-aminopropyltrimethoxysilane (Sigma Aldrich) for 5 min and then for 30 min with 0.5% glutaraldehyde (Electron Microscopy Sciences ...
-
bioRxiv - Molecular Biology 2021Quote: ... followed by banding on a 6-18% Iodixanol gradiant (Sigma D1556) each by 90 mins of ultracentrifugation at 38,000 rpm [19] ...
-
bioRxiv - Genomics 2020Quote: ... alkylated with 18 mM 2-chloroacetamide (Sigma-Aldrich Cat# C0267-100G) and in-gel digested overnight at 37°C with sequencing grade trypsin (Promega Cat# V5111) ...
-
bioRxiv - Systems Biology 2019Quote: ... Secondary incubation with anti-Protein G HRP conjugated (Millipore 18-161) at 1:10,000 was performed at RT during one hour ...
-
bioRxiv - Neuroscience 2019Quote: ... High purity deionized water (ddH2O) was filtered from Millipore (18 OMG). OmniTrace glacial acetic acid and ammonium hydroxide were obtained from EMD Chemicals ...
-
bioRxiv - Microbiology 2020Quote: ... were dissolved in 18:1:1 normal saline:PEG400:Tween80 (Sigma Aldrich) and administered to mice via intraperitoneal injection at a dosage of 0.1-1mg/kg bodyweight four hours prior to behavioral testing or sacrifice.
-
bioRxiv - Cell Biology 2021Quote: ... and 18 mm coverslips were activated using 3-aminopropyltrimethoxysilane (Sigma Aldrich) for 5 min and then for 30 min with 0.5% glutaraldehyde (Electron Microscopy Sciences ...
-
bioRxiv - Molecular Biology 2019Quote: ... briefly in ice-cold 18 MΩ resistant de-ionized H2O (Millipore) and twice in ice-cold CH3COONH4 (1.5 % w/v) ...
-
bioRxiv - Microbiology 2020Quote: ... phosphatidylcholine (PC 16:0-18:1; cat.no.42773, Sigma Aldrich, USA), phosphatidylserine (PS 16:0-18:1 ...
-
bioRxiv - Microbiology 2020Quote: ... phosphatidylethanolamine (PE 16:0-18:1; cat.no.01991, Sigma Aldrich, USA), phosphatidylcholine (PC 16:0-18:1 ...
-
bioRxiv - Microbiology 2020Quote: ... phosphatidylcholine (PC 16:0-18:1; cat.no. 42773, Sigma Aldrich, USA), or a mixture of PC with phosphatidylserine (PS 16:0-18:1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Ovaries were dissociated for 18 minutes in 0.25% Trypsin (Sigma-Aldrich) at 25 °C ...