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Citations for Millipore Sigma :
201 - 250 of 6613 citations for IL 15RA&IL 15 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Cells were blocked with human IgG (1.25 mg/mL, 15 min; Sigma Aldrich). Surface staining of unfixed/unpermeabilized cells employed the following antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... For in vitro experiments human intestinal cell line HCT-15 (Sigma-Aldrich, # 91030712) was used ...
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bioRxiv - Microbiology 2020Quote: ... 15 µl of secondary antibody (anti human Fab-HRP: Jackson ImmunoResearch, cat# 109-035-097; anti human IgG-HRP: Sigma, cat# A6029-1ML ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant ephrins or Fc were preclustered in a 5:1 molar ratio with either mouse or goat anti-human Fc (Millipore Sigma cat. no. 16760 and 12136 respectively) for 30’ at 37°C.
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bioRxiv - Molecular Biology 2021Quote: ... The HE-Fc containing fractions were dialyzed in PBS and concentrated using 30kD Amicon Ultra-15 filters (EMD Millipore). Purified proteins were observed by SDS gel migration as well as anti-human IgG Fc probing by Western ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in a 24-well plate (500’000 cells/well) in complete IMDM supplemented with 6 ng/ml IL-6 (Sigma), 6 ng/ml IL-3 (eBioscience ...
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bioRxiv - Neuroscience 2021Quote: Plasma soluble TREM2 (sTREM2) levels were measured using a Luminex 200 3.1 xPONENT System (EMD Millipore, Chicago, IL, USA) and a custom detection method designed to capture the soluble portion of TREM2 protein as previously described (24) ...
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bioRxiv - Immunology 2019Quote: ... IL-17 and CCL17 were determined with ELISA assays according to the manufacturer’s protocols (Sigma, St. Louis, MO, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were seeded at 1.0E5 cells/ml with 10 ng/ml IL-3 and 8μg/ml polybrene (Sigma-Aldrich). Virus was added to wells at 0 ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with 1/200 diluted Goat Anti human IgG Fc-FITC antibody (Cat: F9512, Sigma) for 45 min and analyzed using flow cytometry (CytoFLEX ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS and human fibroblast growth factor-2 (5 ng/mL, Sigma-Aldrich, St Louis, MO, USA) at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: ... and binding was detected with an anti-human IgG (Fc specific) (1:5000 dilution, Sigma-Aldrich, A0170) or anti-FLAG (1:1000 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Antibodies and dyes used in this study were: Anti-Human IgG Fc specific (Millipore Sigma I2136-1ML), Anti-6X His tag (Abcam ab9108) ...
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bioRxiv - Immunology 2020Quote: ... FCS (Sigma Life Science ...
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bioRxiv - Biophysics 2021Quote: HEK293 cells were cultured in DMEM (Sigma) containing 10% FBS (Life Technologies) ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells were cultured in DMEM (Sigma) with 10% fetal bovine serum (FBS) ...
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bioRxiv - Genomics 2022Quote: ... HEK293 cells were passaged in DMEM (Sigma) supplemented with 10% FBS (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... OEC-2 was cultured on human fibronectin-coated plates (15 μg/ml; EMD Millipore) in serum-free N2B27 medium (DMEM/F12 [Gibco] and Neurobasal [Gibco] 1:1 ratio ...
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bioRxiv - Cell Biology 2020Quote: ... cells were matched-paired and incubated in CO2-free conditions in L-15 medium (supplied with 10% FCS 2mM L-glutamine and 10μg/ml Pen/Strep) and LPS (Sigma-Aldrich) or CSF-1 (Miltenyi Biotec Australia Pte Ltd) ...
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bioRxiv - Cell Biology 2022Quote: ... Growth medium was then removed from apical side and on the basolateral side it was replaced by ALI differentiation medium +/- 10 ng/ml IL-13 (IL012; Merck Millipore). Once the ALI interface was established medium was exchanged every second day till day 25-28 on ALI ...
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bioRxiv - Microbiology 2020Quote: ... the apical medium was removed and basolateral medium was switched to differentiation medium ± 10 ng/ml IL-13 (IL012; Merck Millipore). Differentiation medium consisted of a 50:50 mixture of DMEM-H and LHC Basal (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... 106 cells/ml in 500ul of regular medium supplemented with IL-2 500UI in presence of protamine-sulfate at 20ug/ml (Sigma). The plates were then centrifuged at 1,000 g for 1 h and incubated at 37°C overnight ...
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bioRxiv - Immunology 2022Quote: ... Transient expression of human IL-18BP or IL-18BPΔNΔN was achieved using branched polyethyleneimine (PEI) (Mn ∼ 10 000, Cat.:40,872-7, Sigma-Aldrich, Diegem, Belgium) as transfection reagent (67) ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml recombinant IL-1β (Preprotech) or 100 ng/ml lipopolysaccharide from Escherichia coli O55:B5 (LPS, Sigma-Aldrich) for 6h at 37°C ...
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bioRxiv - Neuroscience 2019Quote: Astrocytes were plated on PO/Lam coated 96 well plates and treated with 3 ng/mL IL-1α (Sigma; 3901), 30 ng/mL tumor necrosis factor alpha (R&D Systems ...
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bioRxiv - Cell Biology 2019Quote: ... We therefore investigated the bioactive LM profiles in tendon-derived stromal cells isolated from the tendons of healthy volunteers and patients in the presence of IL-1β (10 ngmL-1, Sigma) in medium (DMEM F12 phenol red free medium ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) was added ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) was added ...
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bioRxiv - Microbiology 2022Quote: ... according to the manufacturer’s instructions and activated by incubation in the presence of 100 U/ml IL-2 (Sigma Aldrich) and T Cell TransAct™ human (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in the presence of recombinant mouse IL-4 (25 ng/mL; #404-ML, R&D) and LPS (20 μg/mL; #L2880, Sigma) over night to induce B cell activation and terminal differentiation ...
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bioRxiv - Genetics 2022Quote: ... cells were grown in a basal erythroid medium (BEM) supplemented with hSCF, IL-3, EPO (Eprex, Janssen-Cilag) and hydrocortisone (Sigma). From day 6 to 20 ...
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bioRxiv - Immunology 2023Quote: ... Sorted Treg cultures also contained 1000 U/mL of IL-2 (Proleukin) and 50 nmol/L of rapamycin (Sigma- Aldrich), whereas Tconv cultures contained 100 U/mL of IL-2 ...
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bioRxiv - Immunology 2023Quote: ... ex vivo isolated naïve or overnight stimulated (anti-CD40 at 5μg/ml and IL-4 at 1ng/ml) 3×105 B cells were plated on a poly-D lysine (PDL) (Sigma)-coated XF96 cell culture microplate and incubated at 37 °C for a minimum of 30 min in a CO2-free incubator in assay medium (XF RPMI medium pH 7.40 supplemented with 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... and reconstituted to 1 mg/mL in sterile water and diluted to 250 μg/mL (IL-9) or 10 μg/mL (IFNγ and IP-10) in 0.1% cell-culture grade bovine serum albumin (Sigma A9418) in 1X PBS ...
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bioRxiv - Immunology 2024Quote: BMDM were polarized or not with recombinant IL-4 (10 ng mL−1, peprotech) for 24 hours and with LPS (10 ng/ml, Sigma) for 8 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 and 7 post IL-12mRNA/scRNA injection (day 11, 13 and 17 post inoculation) and digested in 30% collagenase (Sigma) for 30 minutes at 37°C ...
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bioRxiv - Bioengineering 2019Quote: ... tissue culture polystyrene culture plates were pre-coated with 20 mg/ml anti-IgG (human, Fc-specific, Sigma) for 1 hour at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... We detected bound antibody with horseradish peroxidase (HRP)-coupled anti-human (Fc) antibody (Sigma Aldrich catalog number A0170).
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bioRxiv - Neuroscience 2020Quote: Human Embryonic Kidney Cells (HEK293) and HEK293 Flp-In T-Rex cells were grown and maintained in Dulbecco’s modified Eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 FlpIn mycBirA*FOPNL and HEK293 FlpIn mycBirA* cells were incubated for 24 h with 50 μM biotin (Sigma-Aldrich) in the presence or absence of 0.1 μg/ml tetracycline.
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bioRxiv - Immunology 2019Quote: ... and HEK293 and HEK293T cells in DMEM (Sigma) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... and HEK293 cells with polyethylenimine (PEI, Sigma Aldrich). Details are described in the SI Appendix ...
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bioRxiv - Neuroscience 2022Quote: HEK293 culture (ECACC 85120602, Sigma-Aldrich, Munich, Germany), transfection and electrophysiological recordings were performed as described previously (Grimm et al. ...
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bioRxiv - Biophysics 2023Quote: HEK293 cells were cultured in RPMI medium (Sigma) supplemented with 10% v/v fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were treated with MG132 (Sigma-Aldrich) at a final concentration of 1 or 10 µM ...
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bioRxiv - Microbiology 2020Quote: ... Thymic fragments were washed in RPMI 2% FCS and digested 15 min at 37 °C in 500 μg/mL Liberase TL (Sigma-Aldrich) and 111 μg/mL DNase I from bovine pancreas (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... 721.221 was purchased from the International Histocompatibility Working Group and grown in RPMI-1640 supplemented with 15% heat-inactivated fetal calf serum (FCS) (Sigma-Aldrich), sodium pyruvate (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... and suspended at 2 x 104 cells/mL in Titan Cell Medium (TCM) composed by 5% Sabouraud, 5% inactivated Fetal Calf Serum (FCS, Biological Industries) and 15 µM sodium azide (Sigma Aldrich), diluted in 50 mM MOPS adjusted to pH 7.3 ...
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bioRxiv - Immunology 2020Quote: ... were cultured at a concentration of 1 million cells per ml in complete media on pre-incubated anti-CD3/anti-CD28 bound plates in IL-2 (20ng/ml) (Sigma-Aldrich) for 2 days and then just IL-2 (20ng/ml ...
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bioRxiv - Physiology 2019Quote: The interactions between TRPA1 and IL-R1 in nodose ganglia cultures were detected by PLA with Duolink in situ kit (Sigma- Aldrich). Primary nodose ganglion cells were harvested and cultured on glass coverslips as described above ...