Labshake search
Citations for Millipore Sigma :
351 - 400 of 457 citations for IKB alpha since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... Alpha-tomatine was purchased from Extrasynthese (Genay, France) and tomatidine from Sigma Aldrich (St. Louis, MO, USA).
-
bioRxiv - Genomics 2024Quote: ... Samples were incubated with primary antibodies (mouse anti-acetyl-alpha tubulin antibody, clone 6-11B-1 [Millipore-Sigma Aldrich ...
-
bioRxiv - Genetics 2020Quote: ... Cells were spun down and resuspended in fresh SC-Dex media containing 24 nM alpha factor (Millipore-Sigma). Cells were incubated at 30°C for 2 hours to induce arrest in G1 phase.
-
bioRxiv - Genetics 2020Quote: ... Cells were spun down and resuspended in fresh SC-Dex media containing 24 nM alpha factor (Millipore-Sigma). Cells were incubated at 30°C for 2 hours to induce arrest in G1 phase.
-
bioRxiv - Cell Biology 2020Quote: ... mouse anti-alpha smooth muscle actin (αSMA) (1:250, Cat. No A5228, Sigma-Aldrich, St. Louis, MO, USA). Then ...
-
bioRxiv - Cell Biology 2020Quote: ... mouse anti-alpha smooth muscle actin (αSMA) (1:2000; Cat. No A5228, Sigma-Aldrich, St. Louis, MO, USA), goat anti-CCN2/CTGF (1;500 ...
-
bioRxiv - Cell Biology 2019Quote: ... Immunoblotting was performed using the following primary antibodies: mouse anti-lys40 acetylated alpha tubulin (1/1000, Sigma, #T6793), mouse anti-alpha tubulin (1/1000 ...
-
bioRxiv - Neuroscience 2021Quote: ... as indicated: antibody against phospho-alpha synuclein (p-aSyn, S129; 81A monoclonal; EMD Millipore, #MABN826; dilution 1:1000)(46) ...
-
bioRxiv - Immunology 2020Quote: ... and the protein was eluted with MES wash buffer supplemented with 500 mM methyl-alpha-D-mannopyranoside (Sigma). The protein was buffer exchanged into phosphate buffered saline by successive rounds of centrifugation and stored at −80°C.
-
bioRxiv - Genetics 2020Quote: ... log phase cells (OD600~0.5 - 0.6) with bar1Δ were arrested in late G1 phase using 50 ng/ml alpha-factor peptide (Sigma) for ~120 minutes ...
-
bioRxiv - Biochemistry 2023Quote: As in previous study [31] Aspergillus oryzae alpha-amylase (EC 3.2.1.1) and potato starch were purchased from Sigma-Aldrich, USA ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Tomatidine HCl (≥ 95%) and alpha-solanine (≥95%) standards were purchased from Sigma-Aldrich (St. Louis, MO, United States).
-
bioRxiv - Neuroscience 2024Quote: ... Absolute and relative activity for the full 24-hour recording and 12-hour periods were also calculated for six different frequency bands (i.e., SWA 0.5-5 Hz, Theta 5-9 Hz, Alpha 9-12 Hz, Sigma 12-16 Hz ...
-
bioRxiv - Cancer Biology 2019Quote: ... Water (>18.2 MΩcm−1 at 25 °C) was obtained from an Alpha-Q Ultrapure water system (Millipore, Bedford, MA) and acetonitrile (AcN ...
-
bioRxiv - Neuroscience 2019Quote: ... source-space power was averaged within standard frequency bands (Delta: 1-4Hz, Theta: 5-8Hz, Alpha: 8.5-12Hz, Sigma: 12.5-17Hz ...
-
bioRxiv - Bioengineering 2021Quote: ... Cells were seeded at 1E6 cells/ml in MEM-Alpha (Biological Industries) supplemented with 10% Heat Inactivated FCS (Sigma) and 50IU rhIL-2 (Peprotech).
-
bioRxiv - Neuroscience 2020Quote: ... Primary antibodies (Anti-Alpha-synuclein - phospho S129, rabbit polyclonal, Abcam, Cambridge, UK; Anti-GAD67, mouse monoclonal, Millipore, CA, USA) were incubated for 48h at 4°C in a 1:1000 dilution ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were fixed for 15min in 4% PFA and stained with α-alpha-actinin antibody (A7811, Sigma, 1:1000).
-
bioRxiv - Cell Biology 2021Quote: ... the mouse anti-alpha-tubulin B-5-1-2 was used at a dilution of 1:500 (Sigma, T5168). Polyclonal rabbit anti-TgCentrin1 (Fig ...
-
bioRxiv - Developmental Biology 2022Quote: ... marrow was flushed with Minimum Essential Medium-alpha (alphaMEM) containing 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO), penicillin and streptomycin ...
-
bioRxiv - Developmental Biology 2019Quote: The primary antibodies and concentrations used were: mouse anti-alpha tubulin (1:500; Sigma-Aldrich, Inc. Cat.# T9026. RRID:AB_477593). Secondary antibodies are listed in the Key Resources table.
-
bioRxiv - Bioengineering 2019Quote: ... AGMS-3 cells were cultured in alpha-MEM (Thermo Fischer Scientific) containing 15% fetal bovine serum (F7524, Sigma-Aldrich), 100 U mL−1 penicillin ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Male C57Bl/6J mice were treated with 5% 4-MU (Alpha Aesar) and 2 mg/ml 4-MUG (Sigma) starting at 8 weeks of age for 20 days ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Male C57Bl/6J mice were treated with 5% 4-MU (Alpha Aesar) and 2 mg/ml 4-MUG (Sigma) starting at 8 weeks of age and maintained on this diet until they were euthanized ...
-
bioRxiv - Plant Biology 2022Quote: ... as well as commercially available mouse anti-alpha-tubulin (Sigma-Aldrich, USA, cat. no. T9026-2, dilution 1:300), rabbit anti-histone H3K4me2 (abcam ...
-
bioRxiv - Neuroscience 2023Quote: ... or high pH medium supplemented with 0,01 μM 7-alpha,25-dihydroxycholesterol (7α,25OHC) (Sigma Aldrich, 64907-22-8) was added to the bottom of the chamber ...
-
bioRxiv - Neuroscience 2022Quote: ... data was separated into pre-defined frequency bins (Delta 0.5-4 Hz, Theta 4-8 Hz, Alpha 8-13 Hz, Sigma 12-15 Hz ...
-
bioRxiv - Biophysics 2023Quote: ... blocked and stained with anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma) overnight at 4°C ...
-
bioRxiv - Bioengineering 2023Quote: ... PFA (Alpha Aesar) for 30 min and permeabilized with 0.2 to 0.5% (v/v) Triton X-100 (Sigma-Aldrich) for 5 min ...
-
bioRxiv - Molecular Biology 2023Quote: ... MCF-7 cells were maintained in alpha modification of Eagle’s medium (αMEM, Wisent) supplemented with 10% Fetal Bovine Serum (FBS, Sigma) and 2 mM L-glutamine (Wisent) ...
-
bioRxiv - Genomics 2024Quote: ... Samples were incubated with primary antibodies (mouse anti-acetyl-alpha tubulin antibody, clone 6-11B-1 [Millipore-Sigma Aldrich, cat# ...
-
bioRxiv - Developmental Biology 2020Quote: L1 larvae were loosely synchronized (using small scale embryo prep) and treated with 10 μg/mL alpha-amanitin (Sigma A2263), as in Butuci et al ...
-
bioRxiv - Genetics 2020Quote: ... cells were either harvested (for D0 timepoint) or medium was changed to differentiation medium (MEM alpha supplemented with 10% FBS, 50 μg/ml sodium ascorbate (Sigma) and 10 mM β-glycerophosphate (Roth ...
-
bioRxiv - Bioengineering 2021Quote: Recombinant human fibroblast activation protein alpha (FAP) expressed in Sf21 cells (400-600 ng/μL) was purchased from Sigma-Aldrich and used at the final concentrations indicated for each experiment ...
-
bioRxiv - Biochemistry 2021Quote: ... standard conditions (ii) with or without Agalsidase alpha (5µl/ml) (Takeda) and (iii) with or without wortmannin (75nM) (Sigma-Aldrich). As for the fibroblasts ...
-
bioRxiv - Biochemistry 2021Quote: ... standard conditions (ii) with or without recombinant enzyme Agalsidase alpha (5µl/ml) (Takeda) and (iii) with or without wortmannin (75nM) (Sigma-Aldrich). Of note ...
-
bioRxiv - Biochemistry 2020Quote: ... was destarched by incubation with amylase (0.2 mL, Termamyl 120 L, alpha-amylase from Bacillus licheniformis – Type XII-A, Sigma-Aldrich) in sodium phosphate buffer (125 mL ...
-
bioRxiv - Systems Biology 2020Quote: ... At time 0 medium was removed from cells and recombinant mouse tumor necrosis factor alpha (TNF-α, Sigma-Aldrich, #T7539) was added at concentrations 0-100 ng/mL as 5-minute pulse ...
-
bioRxiv - Molecular Biology 2020Quote: ... ice cold methanol fixed and 4% dried milk blocked cilia pellets and deciliated cell membranes (excised from Transwell inserts) were labelled for 1 hour at room temperature with a mouse anti-alpha-tubulin antibody (T9026, Sigma) diluted 1:500 in PBST ...
-
bioRxiv - Cell Biology 2021Quote: ... the imaging media with alpha factor was removed and new media with 0.1 mg/ml Pronase E (Sigma P-6911) was added to inactivate any remaining alpha factor ...
-
bioRxiv - Cell Biology 2022Quote: ... Slides were moved to a humidified chamber with primary antibody (anti-acetyl-alpha tubulin, clone 6-11B-1, Sigma, MABT868) diluted in PBX (1:250 ...
-
bioRxiv - Immunology 2022Quote: ... 0.6μL was deposited on a thin layer of an air-dried saturated solution (0.6μL) of the matrix alpha-cyano-4-hydroxycinnamic Acid (4-HCCA, Sigma Aldrich, France) in pure acetone ...
-
bioRxiv - Immunology 2020Quote: ... and for ChIPseq experiments cells were subsequently fixed in PBS with 1 % paraformaldehyde (Alpha Aesar) and 10 % FBS for 10 minutes at room temperature (reaction was quenched with 125 mM Glycine (Sigma)) ...
-
bioRxiv - Biochemistry 2020Quote: P-gp was incubated with a 10x molar excess of membrane scaffold protein (MSP) and a 500x molar excess of 40% L-alpha-phosphatidylcholine (PC) from soybean (Sigma) for 1 hour at room temperature with gentle agitation to facilitate formation of nanodiscs [73] ...
-
bioRxiv - Bioengineering 2022Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with Human Cytokine/Chemokine Magnetic Bead Panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity of each analyte was read using a MAGPIX system (Luminex) ...
-
bioRxiv - Bioengineering 2022Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with Human Cytokine/Chemokine Magnetic Bead Panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity of each analyte was read using a MAGPIX system (Luminex) ...
-
bioRxiv - Genetics 2023Quote: ... membranes were incubated overnight at 4°C with primary antibodies in a 1:1 solution of TBST (TBS + 0.05% Tween20) and LiCor blocking buffer (Rabbit anti-GFP, Torrey Pines Scientific TP401; and Mouse anti-alpha-tubulin, Sigma T5168). Membranes were then washed 5x times with TBST ...
-
bioRxiv - Plant Biology 2024Quote: ... Samples were rehydrated through the same EtOH series and incubated at 37 °C overnight in alpha-amylase (Sigma-Aldrich A4551), 0.3 mg.mL-1 phosphate buffer 20 mM pH 7.0 ...
-
bioRxiv - Bioengineering 2024Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with human cytokine/chemokine magnetic bead panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity (MFI ...
-
bioRxiv - Bioengineering 2024Quote: ... Tumor Necrosis factor alpha (TNF-α)) was carried out with human cytokine/chemokine magnetic bead panel (MilliPlex MAP, Millipore, Sigma). Median fluorescence intensity (MFI ...