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Citations for Millipore Sigma :
451 - 500 of 3551 citations for IGSF11 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... These were transfected into Flp-IN 293 Trex K(AAA)21 wt and ZNF598 ko using Genejuice (Novagen). 24 hours post-transfection ...
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bioRxiv - Cell Biology 2021Quote: TGF-β1 or INT-767 treated HK2 or 293-T cells were harvested in RIPA buffer (Sigma # R0278); 200 µg cell protein in 400 μL volume was added to 2 µg primary antibody and gently rotated at 4°C overnight ...
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bioRxiv - Genomics 2024Quote: ... All Freestyle 293-F cells containing a landing pad were induced with 2 μg/mL doxycycline (Sigma-Aldrich).
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bioRxiv - Bioengineering 2019Quote: ... 100 µg/ml human recombinant Delta-1 or human IgG (Sigma) was added ...
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bioRxiv - Immunology 2023Quote: ... Anti-human IgG HRP antibody (Anti-Human IgG-Peroxidase from Sigma) was diluted 1:30,000 in blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... Transfections of HEK 293T cells were carried out using the X-tremeGene 9 DNA Transfection Reagent (Sigma-Aldrich) as recommended by the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected using a 3:1 polyethylenimine (PEI [branched average Mw ~25,000 Da; Sigma-Aldrich]) to DNA ratio (30:10 μg ...
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bioRxiv - Developmental Biology 2021Quote: ... HEK cell aggregates were placed in the middle of a drop of 20 μl chicken plasma (Sigma-Aldrich) on non-coated HNO3-cover slips ...
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bioRxiv - Biochemistry 2022Quote: ... HEK-293T cells were transfected using a 3:1 polyethylenimine (PEI [branched average Mw ∼25,000 Da; Sigma-Aldrich]) to DNA ratio (30:10 μg ...
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bioRxiv - Biophysics 2022Quote: ... The HEK cells used in this study were found free of mycoplasma infection (Sigma-Aldrich mycoplasma detection kit). These cells were cotransfected with 0.1 - 1 μg of mutant hHV1-TAG plasmid and 0.7 μg of pAnap plasmid (a gift from Dr ...
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bioRxiv - Immunology 2023Quote: ... HEK-293T cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM)/F12 medium (Cat# D8437, Sigma, MO, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Cell Biology 2021Quote: ... 4°C) and the cell pellets washed twice with 10% FCS in PBS and resuspend in Dapi solution (Sigma; 50μg/ml in 10% FCS in PBS; 100μl/well) and stored at 4°C ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were grown to a density of 1 million cells/mL and transfected using 293 transfection free reagent (Millipore) mixed with equal ratios of heavy and light chain encoding plasmids with 250 μg of DNA per one liter of culture ...
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bioRxiv - Immunology 2020Quote: ... pTT3-SARS-CoV-2-S was transfected into 293E cells using 293 Free transfection reagent (EMD Millipore Cat # 72181) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 10% fetal calf serum or FCS (Sigma-Aldrich, St. Louis, MO, USA) as well as 1% antibiotics (penicillin and streptomycin) ...
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bioRxiv - Cancer Biology 2019Quote: ... with 10% Fetal Calf Serum (FCS) (Sigma-Aldrich, St Louis, MO, USA) and 2 mM L-Glutamine (BE17-605F ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Cancer Biology 2020Quote: ... All media were supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich) and all cells were kept at 37°C with 5% CO2 ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... containing 7 % fetal calf serum (FCS) (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), 1% GlutaMAX (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... + 10% fetal calf serum (FCS; Sigma Aldrich, St. Louis, Missouri, United States) on plastic or in PRIME-XV MSC Expansion XSFM medium (FUJIFILM Irvine Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 10% FCS and supplemented when indicated with 1 μM thapsigargin (TG, Sigma).
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bioRxiv - Cancer Biology 2021Quote: ... Digested tissue was re-suspended in 10% FCS (Capricorn) DMEM (Sigma Aldrich), Pen/Strep (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were blocked in 1 % BSA and 4% FCS (Sigma-Aldrich) buffer and incubated for 30 min with primary and secondary antibodies (listed above ...
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bioRxiv - Cell Biology 2020Quote: ... Chemotaxis experiments towards 2.5% FCS and 100 ng/ml HGF (#H9661, Sigma) in DMEM were performed with μ-slide chemotaxis chambers (Ibidi ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10% heat inactivated foetal calf serum (FCS; Sigma Aldrich, Germany), 100 U/ml penicillin and 100U/ml streptomycin ...
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bioRxiv - Microbiology 2022Quote: ... an overlay of DMEM containing 2% FCS and 0.6% Methylcellulose (Sigma-Aldrich) was added to the cells ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10 % foetal calf serum (FCS; Sigma - catalogue no. F-7524), 1,000 units/ml LIF ...
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bioRxiv - Immunology 2020Quote: ... The pellet was resuspended in IMDM containing 2% FCS and formaldehyde (Sigma) was added to a final concentration of 1% ...
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bioRxiv - Pathology 2020Quote: ... 5-azacytidine (5-aza, Sigma-Aldrich; 1 µM in 10% FCS DMEM) for 7 days according to previous publications12 ...
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bioRxiv - Immunology 2019Quote: ... The pellet was resuspended in IMDM containing 2% FCS and formaldehyde (Sigma) was added to a final concentration of 1% ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 10% (v/v) heat-inactivated foetal calf serum (FCS) (Sigma), 2mM (1% v/v ...
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bioRxiv - Immunology 2021Quote: ... with 10% FCS and 2 mM L-Glutamine (Sigma, Cat No. G7513) and 1% penicillin/streptomycin (Invitrogen cat no ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS superior, Millipore, S0615), 2 mM L-glutamine (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10 % (v/v) FCS (Biochrom, Sigma-Aldrich or PAN-Biotech) and 1 % (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... was mixed with unconjugated Fc-antibody (5 µg/ml, Sigma-Aldrich, # I2136) in HBSS and incubated for 30-60 minutes at room temperature with gentle agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... Hybridoma cells were selected in DMEM/10% FCS containing HAT (Sigma, MO) and 1% BM-Condimed (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS; Sigma-Aldrich; St. Louise, MO), penicillin streptomycin glutamine (100 μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Biochemistry 2024Quote: ... the supernatant supplemented with 1% Pen/Strep and 10% FCS (Sigma-Aldrich) and stored in the dark at 4°C.
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bioRxiv - Cell Biology 2020Quote: ... human Akt1 (Sigma-Aldrich) or 0.3 μg active ...
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bioRxiv - Biochemistry 2022Quote: ... Purified human ApoA1 (Sigma) or purified ApoA1-ApoM protein was resuspended in PBS (10:1 mol/mol ...
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bioRxiv - Bioengineering 2019Quote: Human holo Tf (Sigma) was conjugated to AF700 or AF750 (Life Technologies ...
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bioRxiv - Systems Biology 2020Quote: ... human (GluFib, Sigma Aldrich) was added (0.3 ng/µl ...
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bioRxiv - Neuroscience 2021Quote: ... recombinant human apoE4 (Sigma), and ThT (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Human HDL (Merck Millipore) was incubated with NS1 for 1 hour at 37°C prior to the DSC experiments ...
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bioRxiv - Neuroscience 2021Quote: ... human fibronectin (Sigma-Aldrich) 5 µg/ml ...