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Citations for Millipore Sigma :
251 - 300 of 9540 citations for IFN gamma Mouse HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... An inflammatory environment was created by adding the cytokines TNF-α and IFN-γ (Sigma, Burlington MA) to the medium at 15 and 20 ng/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then stimulated with 120 IU/mL of IFN-β (Sigma-Aldrich IF014, St. Louis, MO), 800 IU/mL of IFN-α (Sigma-Aldrich SRP4594 ...
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bioRxiv - Immunology 2023Quote: ... anti-human IFN-γ clone 4S.B3) followed by labelling with 1:1000 diluted ExtrAvidin-Peroxidase (Sigma E2886) and developed by with 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Scientific™).
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bioRxiv - Microbiology 2021Quote: ... Gamma-delta T cells (γδ T cells) were separated from heparinised peripheral blood by density-gradient centrifugation with Histopaque-1077 (Sigma, Spain). Phagocytosis assay was carried out in heparinized whole blood samples incubated with fluorescein (FITC)-labelled opsonized Escherichia coli bacteria using the Phagotest kit (BD Pharmigen ...
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bioRxiv - Cell Biology 2023Quote: ... 24 hours after induction of GFP-KIF18A and endogenous KIF18A knockdown cells were fixed for 3 minutes in - 20oC methanol and subsequently stained for ms-gamma-tubulin 1:500 (Sigma Aldrich, T5326), guinea-pig-CENP-C 1:250 (MBL ...
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bioRxiv - Neuroscience 2023Quote: ... Lipopolysaccharide (LPS serotype O55:B5, gamma-irradiated) and lipoteichoic acid (LTA; from Staphylococcus aureus) were purchased from Sigma (L6529 and L2515, respectively). Recombinant mouse granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Cell Biology 2023Quote: ... 24 hours post-knockdown and induction coverslips were fixed in -20oC methanol for 3 minutes and stained for ms-gamma tubulin 1:500 (Sigma Aldrich, T5326), guinea-pig CENP-C 1:25 (MBL ...
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bioRxiv - Neuroscience 2021Quote: The transfected HEK293 cells were seeded on glass coverslips (Matsunami) coated with poly-L-lysine solution (Sigma-Aldrich) then incubated for 4–10 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Neuro-2a (N2a) and HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) high glucose (Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2019Quote: ... HEK293 cell viability after iOn plasmids transfection was assessed by dye exclusion with Trypan blue solution (0.4%, Sigma). FP expression was either assayed by flow cytometry ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) cells were maintained in a 37°C/5% CO2 atmosphere in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293T and HEK293-FT cells were maintained in DMEM supplemented with 10% FBS,1 mM sodium pyruvate (Sigma), 2 mM L-glutamine (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: HEK293-FLP cells stably expressing UBC:DOR-APEX2 were plated onto poly-L-lysine (Sigma Aldrich, Cat # P8920-100ML) coated coverslips in wells of a 24-well plates ...
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bioRxiv - Microbiology 2023Quote: HeLa cells and HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% foetal bovine serum (Sigma) at 37°C in 5% (V/V ...
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bioRxiv - Immunology 2021Quote: ... cells were supplemented with 50 ng/ml IFN-γ (Stemcell – 78020) and 10 ng/ml LPS (Sigma – L4391) to stimulate M1 polarization ...
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bioRxiv - Microbiology 2019Quote: ... a gamma-irradiated version of LabSupply 5001 Rodent LabDiet) or an equivalent diet with 5% xanthan gum added (95% LabSupply 5001 Rodent LabDiet, 5% xanthan gum [Sigma]; gamma-irradiated by manufacturer). We housed mice in groups of two to five animals per cage ...
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bioRxiv - Biophysics 2021Quote: The cAMP synthesis of heterologously expressed sAC in HEK293 lysates was induced by applying di-sodium-ATP (Sigma Aldrich) and MgCl2 or MnCl2 in isotonic HEPES buffer ...
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bioRxiv - Biochemistry 2020Quote: ... control and SENP3 over-expressed HEK293 cells were stimulated by poly (I:C) for 6h and lysed in NETN buffer supplemented with 10mM PMSF (Sigma). The whole cell lysates were immunoprecipitated with 10 μl Anti-Flag (M2)-agarose (Sigma ...
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bioRxiv - Microbiology 2020Quote: The human embryonic kidney cell lines HEK293 and HEK293T were cultured in Dulbecco’s modified Eagle’s medium (D1152, Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells transfected and grown overnight prior to incubation with 5μg/ml cycloheximide (CHX; Sigma-Aldrich, St. Louis, MO) in the presence and absence of 15μM CQ (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... murine fibroblast (3T3, laboratory stocks) and HEK293 (laboratory stocks) cell lines were maintained in Dulbecco’s minimal essential medium (DMEM, Sigma) supplemented with 10% (v/v ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) and K562 cells in RPMI-1640 medium (Sigma-Aldrich). All media had high glucose concentration and were supplemented with 10% fetal bovine serum and 2 mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
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bioRxiv - Neuroscience 2023Quote: ... were produced in HEK293 cells by mixing lentiviral vector and packaging vectors using branched polyethyleneimine (bPEI) (MW 25Kda, Sigma) as the transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: U2OS osteosarcoma cells and HEK293 (human embryonic kidney) cells were grown in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma, D1152) supplemented with 10% fetal bovine serum (FCS ...
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bioRxiv - Cancer Biology 2019Quote: ... Novus Biologicals NB-100-142, 1:500 dilution; anti-alpha-tubulin, Novus Biologicals NB-600-506, 1:1000 dilution, anti-gamma tubulin, Sigma, T3320, 1:1000 dilution) for 1 hour at 4°C ...
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bioRxiv - Immunology 2021Quote: HEK293 and transfected derivatives were left unstimulated or stimulated for 24h with 10 ng/mL lipopolysaccharide (LPS) from Salmonella (Sigma), 10 ug/mL isolated S protein ...
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bioRxiv - Neuroscience 2020Quote: ... the HEK293 and primary neuronal cells were plated on the 12mm #1 coverslips coated with poly-D-lysine (Sigma-Aldrich). For the 96-well plate screening assays ...
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bioRxiv - Biochemistry 2020Quote: HEK293 cells were seeded at a low density on 0.01% (w/v) poly-L-lysine (Sigma, St Louis, MO, USA) coated coverslips and were transfected the subsequent day using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... The exogenous expression of ACE2 and TMPRSS2 in the HEK293-C34 cells was induced by addition of doxycycline hydrochloride (1 μg/ml) (Sigma). TMPRSS2-expressing Vero E6 (VeroE6/TMPRSS2 ...
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bioRxiv - Molecular Biology 2020Quote: pCDNA3 vectors expressing HA-tagged full size LARP6 or La-domain were transfected into HEK293 cells and 24h after transfection the cells were fixed and incubated with anti-HA primary antibody (Sigma), followed by incubation with fluorescent secondary antibody ...
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bioRxiv - Immunology 2019Quote: ... Vectors were transiently transfected in HEK293-6E cells and antibodies were purified from supernatant using Robocolumn Eshmuno A (EMD Millipore) or Monofinity A Resin prepacked columns (Genscript) ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected with plasmids encoding different ACE2 orthologs (Table S1) by polyetherimide (PEI) (Sigma, St Louis, MO, USA). After 40 hrs incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... thereafter named HEK293) were cultured under 5% CO2 at 37°C in low-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Sigma–Aldrich, D6046) supplemented with 10% foetal bovine serum (FBS-Gibco) ...
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bioRxiv - Immunology 2022Quote: ... Antibody production was conducted by co-transfection of the heavy and light chain expression vectors into Human HEK293 suspension culture cells (Expi293F) using polyethylene mine (Sigma). Cells were subsequently cultured for 4 days before being pelleted by centrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... Then pSIREN-RetroQ-Sirt7 and the negative control vector (pSIREN-RetroQ) were transfected into HEK293 cells using PEI transfection reagent (Sigma). The pSIREN-RetroQ-Sirt7 and the negative control vector (pSIREN-RetroQ ...
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bioRxiv - Biochemistry 2023Quote: HEK293 Flp-In T-Rex cells were cultured in DMEM high glucose medium supplemented with 10% FBS (Sigma-Aldrich F0804), 2mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: PFKFB3 depletion was carried out in HEK293 cells using siRNAs (80 nM; transfected twice on consecutive days) from SIGMA (SASI_Hs01_00065120). The cells were treated with control or specific siRNA and lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: 2×10^5 Vero E6 cells or HEK293-ACE-2 were cultured in 24-well plates in Dulbecco’s Modified Eagle medium (DMEM, Sigma D5796) containing 10% of fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... and GIGYF2-KO TLR3-HEK293 cells were either mock-treated or transfected with 1 μg/mL Poly(I:C) (Sigma, P1530) using Lipofectamine2000 ...
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bioRxiv - Cell Biology 2024Quote: Doxycycline-inducible stable Flp-In T-REx HEK293 cell lines expressing mitochondrial-relocated golgin-BirA* fusion proteins were induced with 1 μg/ml doxycycline (Sigma) in culture medium once they reached approximately 80% confluency ...
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bioRxiv - Genetics 2019Quote: ... from HEK293 cells stably expressing p3XFLAG-ADCK4 or -BAP were incubated with 80 μL of FLAG M2 agarose beads (Sigma-Aldrich) for 48 h at 4°C in an orbital shaker ...
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Cell Biology 2021Quote: ... GBM#U3013 cells were infected with lentiviral particles generated by transfecting HEK293 cells with pLKO.1-puro plasmids from the Mission shRNA library (Sigma-Aldrich). Briefly ...
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bioRxiv - Immunology 2021Quote: ... HEK293 and HEK293T cells (gifts from Caetano Reis e Sousa, The Francis Crick Institute, UK) were maintained in DMEM (Sigma-Aldrich) containing 4.5 g/L glucose ...
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bioRxiv - Immunology 2022Quote: Luciferase-expressing HeLa or HEK293 cells were seeded into a U-bottomed 96-well plate plus D-Luciferin (75 μg/ml; Sigma-Aldrich) for cytotoxicity assessment ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cells were cultured in serum-free and phenol red-free media containing 4.5 g/L glucose (Sigma-Aldrich, Dorset, UK), 1% penicillin/streptomycin ...
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bioRxiv - Biochemistry 2019Quote: Cell lysate from HEK293 transfected with FLAG-Podocin (WT, R45A, R50 and R54A) was immobilized on anti-FLAG beads (Sigma-Aldrich). The beads were washed with IP buffer (1%–2% Triton-X ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells stably expressing CCR5 were labeled with 2ug/ml rabbit anti-Flag tag antibody (F7425, Sigma, St. Louis, MO, USA) in FACS buffer (PBS with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Cancer Biology 2023Quote: ... Miapaca2, Hek293 were cultured with high-Glucose DMEM medium (Fisher, SH30423FS) supplemented with 10% FBS and 1% Pen-Strep (Sigma, 11074440001). The BxPC3 cell were cultured with RPMI1640 (Fisher ...