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Citations for Millipore Sigma :
451 - 500 of 3776 citations for Human MXRA8 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2021Quote: ... SC –leu-trp plates and SC –leu-trp-his + 10mM 3-aminotriazole (Sigma) plates ...
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bioRxiv - Neuroscience 2020Quote: ... and HIS dihydrochloride (Sigma-Aldrich; 10 μg/10 μl; 9 mM; 90 nmol), as well as a combination of 2 substances (ALA 90 μg with BAM8-22 1 μg in 10 μl ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... which coeluted with His-TrxA or monoclonal anti-polyHistidine-Peroxidase antibody (Sigma-Aldrich) for the detection of recombinant His-tagged TrxA ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Guanidine Hydrochloride (Serva, 24205.02) via Hi-Trap Desalting column (Sigma Aldrich GE17140801) for further experiments ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with 15 ml HIS-Select Nickel Affinity Gel (Sigma) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-Pap1 were pooled and concentrated using 30,000 MWCO concentrators (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Microbiology 2024Quote: ... followed by western blotting was using anti-His primary antibody (Sigma-Aldrich, H1029) and anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The medium was then loaded on a His-select Nickel affinity column (Millipore) equilibrated in 50 mM NaH2PO4 ...
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bioRxiv - Immunology 2024Quote: ... 10X hi ovomucoid inhibitor stock solution contained 300 mg BSA (Sigma-Aldrich, #A8806), 300 mg ovomucoid trypsin inhibitor (Worthington ...
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bioRxiv - Cell Biology 2019Quote: ... S-protein-agarose beads and S-tag antibody were purchased from EMD Millipore. Endo H ...
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bioRxiv - Microbiology 2019Quote: ... The poly-histidine tag was removed using the Thrombin cleavage kit (Millipore-Sigma) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The poly-histidine tag was removed using the Thrombin cleavage kit (Millipore-Sigma) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Iss10 constructs were cloned as N-terminal Strep-tag fusions into pACYCDuet (Novagen). Combinations of Red1 and Iss10 constructs were co-expressed in E ...
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bioRxiv - Developmental Biology 2019Quote: ... The ChIP antibody was an anti-myc tag polyclonal antibody from Sigma-Aldrich (C3956 ...
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bioRxiv - Cell Biology 2020Quote: ... lysates were incubated with 50 μL of Myc-tag beads (A7470, Sigma-Aldrich, blocked with 5% BSA for one hour at room temperature) ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag was proteolytically removed using 5 Units of thrombin (Sigma-Aldrich) per mg protein overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... the GST tag was cleaved using the Thrombin CleanCleave Kit (Sigma-Aldrich, SLBZ7194) according to the manufacture’s protocol after elution step ...
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bioRxiv - Biochemistry 2020Quote: ... the His6-tag was proteolytically removed using 5 U of thrombin (Sigma-Aldrich) per mg protein overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Western blotting was performed using anti S-tag (Cat no. 71549-3, Novagen) and anti His-tag antibody to detect protein-protein interaction.
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal HA-Tag and Mouse Monoclonal Flag antibodies purchased from Sigma (USA); Goat anti-mouse IgG horseradish peroxidase-conjugated secondary antibody and goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody purchased from Thermo Fisher Scientific (USA).
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bioRxiv - Biophysics 2021Quote: ... Protein was expressed as a His6-tag fusion in BL21(DE3) trxB (Novagen) using autoinduction24 ...
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bioRxiv - Cell Biology 2021Quote: ... containing 50 μM Phos-tag AAL-107 (Wako) and 10 mM MnCl2 (Sigma) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... cells were incubated with an anti-HA epitope tag rabbit pAb (H6908, Sigma) and ...
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bioRxiv - Microbiology 2021Quote: ... the Fc tag of all APN orthologs was removed using thrombin (Millipore Sigma) in a reaction mixture containing ...
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bioRxiv - Microbiology 2019Quote: ... or rat horseradish peroxydase-conjugated antibody against HA-Tag (Clone 3F10, Sigma-Aldrich). Quantifications were performed by means of the luminescence image analyzer Image Quant Las 4000 mini and Image Quant software (GE Healthcare).
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bioRxiv - Neuroscience 2019Quote: ... Sections were stained with antibodies against the HA-tag (1:500; Sigma; RRID:AB_260070), pSTAT3 (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... the 2xStrep tag was not removed by incubation with HRV 3C protease (Millipore), and proteins were not purified by size exclusion chromatography.
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bioRxiv - Microbiology 2020Quote: ... then the 2xStrep tag was removed by cleavage with HRV 3C protease (Millipore) overnight ...
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bioRxiv - Physiology 2020Quote: ... or mouse anti-S-tag (for S-tagged NTD, 1:1500, Sigma, SAB2702204) and rabbit anti-Nav1.5 (1:150 ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies against Flag-tag (F3165, 1:3,000) was obtained from Sigma-Aldrich.
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bioRxiv - Cell Biology 2022Quote: Enzymatic cleavage of the GST tag was performed using thrombin (Cat# T1063, Sigma). Supernatants containing cleaved target proteins were incubated with Benzamidine Sepharose 4 flat flow (Cat# 17-5123-10 ...
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bioRxiv - Molecular Biology 2023Quote: ... for the detection of the FLAG tag (A8592, Sigma-Aldrich, clone M2, monoclonal), PAP (P1291 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-FLAG-tag (1:5,000, mouse mAb, F1804, Sigma-Aldrich, St. Louis, MO), anti-alpha-tubulin (1:2,000 ...
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bioRxiv - Cell Biology 2023Quote: ... and the GST tag was removed by cleaving with PreScission Protease (Sigma-Aldrich). The purified proteins were exchanged to the biotinylation buffer containing 25 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... Triacylglycerol (TAG, Cat# T7140) and monoacylglycerol (MAG, Cat# M2015) were purchased from Sigma. β-Glucosylceramide (β- GlcCer ...
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bioRxiv - Microbiology 2023Quote: Antibodies were as follows: commercial mouse monoclonal antibodies to Flag-tag (M2; Sigma), Strep-tag (4F1 ...
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bioRxiv - Bioengineering 2022Quote: ... human iPSC-derived Brain Microvascular Endothelial-like cells were seeded in the vascular channel at a density of 14 to 16×106 cells/mL using human serum-free endothelial cell medium supplemented with 5% human serum from platelet-poor human plasma (Sigma) and allowed to attach to the membrane overnight ...
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bioRxiv - Immunology 2020Quote: ... 100 µL of biotinylated secondary antibody diluted in PBS-BN (anti-human IgG, B1140, 1/1250; anti-human IgM, B1265, 1/1000; or anti-human IgA, SAB3701227, 1/500; Sigma) were added to all wells and incubated for 45 min at 600 rpm at RT and protected from light ...
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bioRxiv - Immunology 2022Quote: Cells were blocked in human blocking buffer (5% human serum (Sigma-Aldrich), 1% rat serum (Sigma-Aldrich) ...