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Citations for Millipore Sigma :
701 - 750 of 4285 citations for Human KIRREL shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... DM cells were infected by shRNA lentivirus twice via spinoculation in the presence of 5 μg/mL polybrene (Sigma-Aldrich). 72 hours post transduction ...
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bioRxiv - Immunology 2023Quote: The shRNA oligos were designed from the Broad Institute Genetic Perturbation platform and the shRNA clones in pLKO.1 were purchased from Sigma. The three oligo sequences for pLKO.1-shSAMD9L are ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse NAFs were transduced using lentiviruses containing shRNA constructs against Postn with 10 mg/ml polybrene (Sigma, TR-1003-G). shPostn1 (F primer ...
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bioRxiv - Cell Biology 2024Quote: ... GFP-PMCA4bLA and PMCA4-specific shRNA expressing MCF-7 lines were mixed with cold Matrigel (Corning Matrigel, DLW356231, Sigma-Aldrich) and 15 µl of cell-Matrigel mixtures were added to removable 8-well chambers (80841 ...
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bioRxiv - Neuroscience 2023Quote: ... TSC1 or AMPK knockdown in RPE1 cells were achieved by infecting cells with viruses derived from pLKO-1 harboring the shRNAs against TSC1 (TRCN0000010453 and TRCN0000039734, Sigma) or AMPK (TRCN00000196482 and TRCN00000219690 ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were purified using a GenElute HP Plasmid MidiPrep Kit (Sigma-Aldrich). To generate plasmids for CRISPR/Cas9 KO cell lines ...
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bioRxiv - Microbiology 2021Quote: ... plasmid DNA was isolated using GenElute ™ Plasmid Miniprep Kit (Sigma-Aldrich) and sequenced using the M13 primers ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Plasmid was then extracted by GenElute™ Plasmid Miniprep Kit (Sigma-Aldrich) and verified by DNA sequencing (BMR Genomics ...
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bioRxiv - Biophysics 2020Quote: ... Plasmids were purified using a GenElute HP Plasmid MidiPrep Kit (Sigma-Aldrich). The tgLAT construct(20 ...
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bioRxiv - Immunology 2020Quote: ... Plasmids were purified with the GenElute Plasmid DNA Miniprep Kit (Sigma-Aldrich), quantified using the Qubit fluorometer (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid was isolated using “GenElute™ Plasmid Midiprep” kit (Sigma Aldrich) and digested with SmaI (Thermo Scientific)
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bioRxiv - Neuroscience 2022Quote: Plasmid DNA was purified with the GenElute HP Plasmid miniprep kit (Sigma) and microinjected in the worm gonad according to a standard protocol (Evans ...
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bioRxiv - Molecular Biology 2022Quote: ... plasmids were purified using the GenElute HP Plasmid Midiprep Kit (Sigma-Aldrich). All of the L1 expression plasmids contain a retrotransposition-competent L1 (L1.3 ...
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bioRxiv - Microbiology 2023Quote: ... Afterward the plasmid was isolated using GenElute plasmid miniprep kit (Sigma Aldrich). The recipient strains (AMp07 and WSM419 ...
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bioRxiv - Plant Biology 2019Quote: Plasmid pFLAG-ATS (Sigma) was used for protein expression in this study ...
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bioRxiv - Molecular Biology 2019Quote: pLKO.1 plasmids (Sigma) encoding shRNA for Sertad4 (TRCN0000247967 and TRCN0000247969 ...
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bioRxiv - Microbiology 2020Quote: ... pET-32a plasmid (Novagen) was used ...
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bioRxiv - Microbiology 2023Quote: ... the pET28a plasmid (Novagen) was used ...
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bioRxiv - Microbiology 2023Quote: Plasmid pSTBlue-1 (Novagen) was used as the vector for routine DNA manipulations ...
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bioRxiv - Cancer Biology 2021Quote: ... SMO-inhibitor resistant Daoy cells with shRNA-mediated knockdown of SUFU had been generated in our lab using the TRCN0000019466 construct (Sigma-Aldrich) [52].
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bioRxiv - Cancer Biology 2021Quote: ... To deplete mouse STK3 expression in HMVP2 cells TRC2 MISSION shRNAs in pLKO.1-puro vector backbone were used (Millipore-Sigma). Lentivirus was generated with psPAX2 and pMD2.G packaging plasmids using with the Polyplus jetPRIME transfection kit in 293T cells ...
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bioRxiv - Cell Biology 2020Quote: ... or human NUP153-specific shRNA lentivirus particles overnight at 37°C followed by selection in medium containing Puromycin (Puro, 2 μg/ml) (Sigma-Aldrich) for 48 hr ...
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bioRxiv - Cell Biology 2020Quote: Lentiviral particles were either generated by the laboratory (NF-κB or p53 reporter) or obtained from the MISSION shRNA library (Sigma-Aldrich). These obtained lentiviral particles harbor the following shRNA clones in pLKO.1 backbone vector and were used to infect BJ cells ...
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bioRxiv - Developmental Biology 2020Quote: ... and RSV-Rev) and a SERPINE1 shRNA construct encoded in a PLKO.1 vector (henceforth called shPAI1: TRCN0000370159, sense: ACACCCTCAGCATGTTCATTG; Sigma-Aldrich). A control PLKO.1 vector containing a scrambled shRNA (Addgene 1864 ...
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bioRxiv - Molecular Biology 2021Quote: J1 mES cells with doxycycline-inducible short hairpin RNA-micro RNA (shRNA – Supplementary Table 2) sequences targeting Tet1 or Tet3 were treated for 48h with 2 μg/mL doxycycline (D9891, Sigma Aldrich).
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bioRxiv - Neuroscience 2019Quote: ... Five 15 cm dishes of 70 – 80 % confluent mouse Schwann cells were separately transfected with 30 μg of pLKO.1-puro vector containing five commercial VDAC1 shRNA (Sigma-Aldrich, sh1#TRCN0000012388 ...
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bioRxiv - Cancer Biology 2019Quote: ... the PDCD4 shRNA oligonucleotides (5‘-CTGGACAGGTGTATGATGTGG-3’) were synthesised and cloned into the MISSION® TRC2 pLKO.5-puro Empty Vector (SHC201, Sigma) by TsingKe Ltd ...
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bioRxiv - Cancer Biology 2020Quote: Using the custom barcodes lentiviral shRNA library, PDX lines (PATC69, PATC124, PATC53 and PATC153) were transduced in vitro using 8 μg/mL Polybrene (Sigma-Aldrich). Libraries were transduced at 1000X coverage and multiplicity of infection (MOI ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentiviral plasmids containing shRNAs targeting STING (shSTING-1 #TRCN0000161345, shSTING-2 #TRCN0000163029) or non-targeted (scrambled) shRNA (shNT #SHC016-1EA) were purchased from Sigma Aldrich. STING-IRES-GFP and DN-IRES-GFP lentiviral plasmids were generated by inserting the cDNA of STING or STING-DN ...
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bioRxiv - Microbiology 2019Quote: Lentiviral particles carrying an anti-CTIF shRNA were produced in HEK293 cells by transfecting a commercially available pLKO.1 vector containing the shRNA sequence targeting the 3’-UTR of the CTIF mRNA (Sigma-Aldrich), pVSVg and psPax2 ...
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bioRxiv - Cancer Biology 2021Quote: LCN2 stable knockdown clones were generated in SUM149 or MDA-IBC3 cells by using shRNA (shLCN2-1: TRCN0000060289 from Sigma-Aldrich; shLCN2-2 ...
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bioRxiv - Immunology 2021Quote: ... pLKO.1 empty vector was from Open Biosystems, and pLKO.1 control shRNA (scramble shSCR, SHC002 and non target shNTgt, SHC016) from Sigma-Aldrich. Lentiviral vectors carrying these constructs were produced by calcium phosphate transfection of 293FT cells with shRNA constructs in combination with packaging vectors psPAX2 ...
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bioRxiv - Cell Biology 2020Quote: ... TRCN0000073737 5’-CCGGCGCGTTATCAACTGGATCCAACTCGAGTTGGATCCAGTTGATAACGCGTTTTTG-3’ designed and cloned into the lentiviral pLKO.1 puromycin resistant vector Mission shRNA lentiviral Transduction particle (Sigma Aldrich). Control Caco2 clones (shNT ...
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bioRxiv - Cell Biology 2021Quote: Mission plasmids directing expression of shRNAs targeting PlexinA2 (TRCN0000061499 (ShPlexA2#1) and TRCN0000061501 (ShPlexA2#2)) or PlexinA4 (TRCN0000078683) were purchased from Sigma Aldrich. The production of the lentiviruses ...
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bioRxiv - Developmental Biology 2022Quote: 300k SV-HUC-1 cells were seeded and transfected with 3µg MISSON pLKO.1-puro non-mammalian targeting control shRNA (Sigma, Cat# SHC002) or shExoc5 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Scrambled and GAN KO cell lines were generated by transducing SH-SY5Y cells with lentivirus expressing the Mission pLKO.1 backbone for scrambled shRNA (Sigma, #SHC002) and GAN shRNA (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Filtered lentiviral supernatant was introduced to MRC5 cells for FLAG-Raf1 experiments or HFF cells for shRNA experiments in the presence of 5 μg/ml Polybrene (Millipore Sigma). Three hours later ...
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bioRxiv - Neuroscience 2023Quote: ... commercially available lentiviral small hairpin RNA (shRNA) vectors based on the pLKO.1 backbone (s. Key Resource Table; annotated as “transfected”) were purchased from Sigma-Aldrich. To reduce the amount of DNA needed for transfection to perform pHluorin assays ...
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bioRxiv - Cancer Biology 2022Quote: ... and the corresponding empty vector (Genecopoeia. MDK silencing was performed by lentiviral-driven expression of shRNAs, with pLKO-constructs purchased from Sigma Aldrich as previously reported16,17,65 ...
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bioRxiv - Neuroscience 2024Quote: SH-SY5Y cells with doxycycline-inducible TDP-43 shRNA expression were treated with 5 μg/mL actinomycin D (Sigma-Aldrich, A1410) for 0 ...
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bioRxiv - Immunology 2023Quote: ... A lentiviral vector expressing shRNA against MDA5 to knock down MDA5 expression in CHME-5xISRE-Nluc (see below) was purchased from Sigma (TRCN0000232948). Lentiviral particles were generated by co-transfection of HEK293T cells with lentivectors (pDuet 5xISRE-Nluc ...
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bioRxiv - Cancer Biology 2023Quote: ... HNT-34 and OCI-AML3 cells were transduced with shRNA-CD81 or non-targeting (NT) shRNA lentiviral vectors (TRCN0000300291[sh291], TRCN0000300293[sh293], TRCN0000300433[sh433] or TRC2 pLKO.5-puro; Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: ... To boost the expression of the shRNA constructs the mice were also provided with 25 mg/kg doxycycline hydrochloride (#D3447, Sigma-Aldrich) resuspended in PBS via intraperitoneal injection once a day for the first two days of doxycycline treatment.
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bioRxiv - Cancer Biology 2024Quote: ... the cells were infected with the lentivirus expressing shRNA or cDNA in the presence of polybrene (5 μg/mL) (Sigma, USA), followed by selection with puromycin (5 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: Human EndoC-βH1 and rat INS1E beta cells were cultured as previously described [45] and incubated with lentiviruses expressing an NNAT-targeting shRNA (Sigma-Aldrich) or Silencer Select siRNAs (Ambion) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were seeded in 60 mm dishes at 50-60% confluency and transiently transfected with CEP41 shRNA (5’-GCTTACAGTTACCCAATTGCA-3’, TRCN0000143499, Sigma-Aldrich) or scramble shRNA (SHC002 ...
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bioRxiv - Microbiology 2020Quote: ... Human serum-supplemented medium was made through addition of complete human serum (Sigma Aldrich) into CDM to a final concentration of 5% (v/v) ...