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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Human Immunodeficiency Virus Reverse Transcriptase Protein HIV 1 Clade B IIIB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and J-Lat 11.1 (integrated full-length HIV-1 genome mutated in env gene and GFP replacing Nef) cells were cultured in RPMI-1640 medium (Sigma) supplemented with 10% FBS and 100 μg/ml penicillin-streptomycin at 37 °C in a humidified 95% air-5% CO2 atmosphere ...
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bioRxiv - Biophysics 2021Quote: ... 293T cells were transfected with 2.5 μg of ΔEnv IN- HIV-1 plasmid (DHIV3-GFP-D116G) (31) using 10 μg of polyethylenimine (PEI, branched, MW ~25,000, Sigma-Aldrich). After 20 h ...
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bioRxiv - Cell Biology 2020Quote: ... at 1:1000 dilution and monoclonal antibody B-5-1-2 (Sigma) at 1:1000 dilution to detect α-tubulin as loading control.
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bioRxiv - Biochemistry 2021Quote: ... α-TUBULIN (ms, clone B-5- 1-2, Sigma T9026, 1:5000), rabbit IgG (gt ...
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bioRxiv - Cancer Biology 2019Quote: ... α-tubulin mouse (1:10000 WB, clone B-5-1-2, Sigma-Aldrich), p120 catenin mouse (1:1000 WB ...
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bioRxiv - Molecular Biology 2021Quote: ... HEC-1-B cells were cross-linked with 1% glutaraldehyde (Sigma-Aldrich) and quenched with 1 ml of 1.25 M glycine for 5 min ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse tubulin (clone B-5-1-2, Sigma, T5168-.2ML; 1/5000), and p21 Waf1/Cip1 (clone 12D1 ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-Tubulin (1:3,000; Sigma clone B-5-1-2, T5168), and mouse anti-b-Actin (1:1,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... the DAC-pretreated (D3R3) cells were treated with 1 μg/mL cytochalasin B (Cyto B; C6762, Sigma-Aldrich) for 1.5 hours prior to coculture with γδ T cells.
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bioRxiv - Immunology 2022Quote: ... Virus was absorbed for 1 hour at 37 degrees C before 1% (w/v) methylcellulose (Sigma-Aldrich) (10 g methylcellulose in 1L MEM (Corning) ...
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bioRxiv - Immunology 2023Quote: ... Human platelets were stimulated with 1 U ml−1 Thrombin from human plasma (Sigma-Aldrich) or left unstimulated for 30 min to assess the purity and activation of isolated cells ...
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bioRxiv - Bioengineering 2019Quote: ... 100 µg/ml human recombinant Delta-1 or human IgG (Sigma) was added ...
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bioRxiv - Plant Biology 2021Quote: ... The protein was eluted with buffer C (buffer B+10%glycerol+2.5 mM Desthiobiotin (Novagen)) in four column volumes ...
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bioRxiv - Cell Biology 2019Quote: ... Doxycycline-inducible OKSM virus (Millipore, SCR512) and m2rtTA virus (Cyagen Biosciences ...
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bioRxiv - Bioengineering 2019Quote: ... Lyophilized proteins were purchased: human serum albumin (HSA; from human plasma, ≤0.02% Fatty acids, Lot #SLBZ2785, Millipore Sigma) and fibrinogen (FBG ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... 1 μL of virus lysate was treated with 250 U of benzonase nuclease (Sigma) at 37°C for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... The virus/antibody mixture was replaced with an overly media containing 1% CMC (Sigma) before incubation for 24 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... and 1:4 800 000 in virus growth medium (VGM) containing MEM (Sigma, 2279), 2 % FBS ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
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bioRxiv - Microbiology 2022Quote: ... effect of HIV on cell types was also assessed in the presence of the non-nucleoside reverse transcriptase inhibitor (NNRTI) Efavirenz (EFA) (Sigma-Aldrich #SML0536) or the integrase strand-transfer inhibitor Raltegravir (RAL ...
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bioRxiv - Neuroscience 2023Quote: ... two μg of RNA were utilized for cDNA synthesis using MMLV-reverse transcriptase and oligo-dT (Sigma: M 1302 and O4387, respectively). A CFX96 Real-Time PCR Detection System with a C1000 Thermal Cycler (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: Jurkat cells and J-Lat 11.1 cells (Jurkat cells containing integrated full-length HIV-1 genome mutated in env gene and GFP replacing nef) 39 were maintained in RPMI-1640 medium (Sigma Aldrich) supplemented with 7% FBS and 100 μg/ml penicillin-streptomycin at 37 °C in a humidified ...
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bioRxiv - Biochemistry 2022Quote: NAD total levels in MT-4 cells and MT-4 cells infected by HIV-1 were assessed using the NAD/NADH Quantification Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... ∼106 HEK 293T cells were cultured in a 15 cm dishes and transfected with 2.5 µg of Env-defective variants of the full-length HIV-1 construct R9 using 10 µg of polyethyleneimine (PEI; branched; MW ∼25000; Sigma-Aldrich). After 20 h ...
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bioRxiv - Biophysics 2023Quote: ... 10 µL of isolated HIV-1 cores were incubated for 45 min at room temperature on hexamethyldisilazane (HMDS)-coated microscope glass slides (Sigma-Aldrich) in a mildly humid chamber ...
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bioRxiv - Neuroscience 2019Quote: ... and anti-α-Tubulin (mouse monoclonal [B-5-1-2], 1:8,000, Sigma).
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bioRxiv - Cancer Biology 2021Quote: ... mouse α-Tubulin (Sigma, B-5-1-2, # T5168, used at 1:5000), mouse α-FLAG (Sigma ...
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bioRxiv - Genomics 2023Quote: ... and Cap B (B1:B2 1:1; BI0347-0505, BI0349-0505 Sigma-Aldrich) reagents ...
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bioRxiv - Cell Biology 2023Quote: ... mouse anti-α tubulin (T5168, B-5-1-2, Sigma-Aldrich, 1:1000). Rabbit anti-Solo antibody was purified using an immunogen peptide conjugated-Sepharose column from the antiserum ...
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bioRxiv - Molecular Biology 2021Quote: Anti-α-tubulin (Sigma-Aldrich, clone B-5-1-2), anti-METTL18 (PROTEINTECH GROUP ...
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bioRxiv - Cell Biology 2019Quote: ... phalloidin-tetramethylrhodamine B isothiocyanate (Sigma, P1951, used at 1:500), DAPI (Sigma ...
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bioRxiv - Systems Biology 2021Quote: ... a 1 mL bolus of anti-foam B (Sigma Aldrich) was injected into the bioreactor to control excessive foam formation ...
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bioRxiv - Microbiology 2021Quote: ... and mouse mAB anti-b-actin (1:5000, A5316 Sigma) diluted in blocking buffer overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... protease and phosphatase inhibitors and 1:5000 Antifoam B (Sigma)) ...
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bioRxiv - Molecular Biology 2020Quote: ... protease and phosphatase inhibitors and 1:5000 Antifoam B (Sigma)) and TEV500 protease cleavage buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μM Latrunculin B (to sequester actin monomers; Millipore, 4280201MG), or an equal volume of ethanol ...
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bioRxiv - Biophysics 2020Quote: ... Mouse anti-α-tubulin (B-5-1-2) (T5168; Sigma), Mouse anti polyglutamylated tubulin ...
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bioRxiv - Synthetic Biology 2019Quote: ... supplemented with 1% penicillin/streptomycin/amphotericin B (PSA) (Sigma Aldrich) and SFT cytokines ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-alpha-tubulin B-5-1-2 (Sigma, T5168), mouse anti-alpha-tubulin TAT-1 (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... 100 U ml−1 of polymyxin B sulfate (Sigma-Aldrich), and 250 mg ml−1 of gentamicin (Life Technologies) ...
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bioRxiv - Developmental Biology 2022Quote: ... and B-Actin peroxidase preconjugated antibody (1:50,000, Sigma, A3854). Membranes were then imaged and analyzed using Licor Image Studio™ software ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti-alpha tubulin (Merck-SIGMA, clone B-5-1-2) was used as a loading control at 1:10000.
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bioRxiv - Molecular Biology 2023Quote: ... anti-α-tubulin MAb (clone B-5-1-2; Sigma) was used at a 1/4,000 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... anti-Tubulin (clone B-5-1-2) from Sigma Aldrich and anti-MUC5AC (clone 45M1 ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.01 mg ml-1 of hygromycin B (Sigma-Aldrich) were used as a culture medium for BJ5ta cells ...
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bioRxiv - Microbiology 2023Quote: ... and rabbit-anti-LC3A/B (1:1000; Sigma-Aldrich, L8918). Band intensities were quantified using Image Quant TL (GE Healthcare) ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-α-Tubulin (B-5-1-2, Merck Millipore/Sigma) antibodies were purchased ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-α-Tubulin (B-5-1-2, Merck Millipore/Sigma) antibodies were purchased ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with mouse b-actin (Sigma #A2228, 1:2000). Following washes ...