Labshake search
Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade A KNH1144 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... 100 μl of virus suspension and Polybrene (hexadimethrine bromide, Sigma) at a final concentration of 8 μg/ml were added ...
-
bioRxiv - Microbiology 2023Quote: ... virus was filtered using a millipore filter (Millipore Sigma, SE1M179M6). On day of infection ...
-
bioRxiv - Plant Biology 2023Quote: ... and then the tobacco etch virus (TEV) protease (Sigma-Aldrich) was added to remove the purification tags ...
-
bioRxiv - Microbiology 2023Quote: ... Virus propagation was performed in VeroE6 cells in DMEM (Sigma) supplemented with 2% fetal calf serum (Logan) ...
-
bioRxiv - Microbiology 2023Quote: Swabs were stored in virus cultivation medium (Sigma-Virocult®). Archived swabs and organ samples were kept at-70 °C until final analyses ...
-
bioRxiv - Cell Biology 2020Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich), and 20 ng/mL human bFGF (Life Technologies)] for 14 to 35 days ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-human vinculin (mouse monoclonal, VIN-1, Sigma Aldrich, V9131; WB 1:1000), anti-human Hic-5 (mouse monoclonal ...
-
bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich), and 20 ng/mL human bFGF (Life Technologies)] for 14 to 35 days.
-
bioRxiv - Cell Biology 2022Quote: ... The proteins were digested by trypsin/lysC (protein: enzyme = 25:1, Millipore) at 37°C overnight ...
-
bioRxiv - Microbiology 2020Quote: ... membrane-extracted proteins were incubated for 1.5 hours at 4°C with purified human IgG (Sigma) that had been previously coupled to CNBr-activated Sepharose beads (GE Healthcare) ...
-
bioRxiv - Immunology 2023Quote: ... Recombinant lyophilized proteins were resuspended using 0.1% (w/v) human serum albumin (HSA) (A9731, Sigma-Aldrich) in sterile water ...
-
bioRxiv - Immunology 2021Quote: ... a sandwich ELISA was used in which 1% Trixon X-100-treated virus lysates were added to plates pre-coated with ConA (50 µg/ml, Sigma) and reacted with anti-Env mAbs 66 ...
-
bioRxiv - Microbiology 2021Quote: ... Virus (genome equivalent of a MOI of 1) was added in the presence of 8 μg/ml polybrene (Sigma Aldrich) and the cells were incubated on ice for 1⍰ h to allow virus binding ...
-
Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... the assay was performed on cells exposed to Pronase (3,5mg/ml) or using virus pre-exposed to 1 to 100 U/mL heparin (Sigma-Aldrich). The role of mannose receptor was evaluated by incubating cells with HIV-1 in the presence of 100µg/mL BMA (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2020Quote: ... Surviving midges and mosquitoes at day 10 were pooled (n=5-10) in 1 ml virus transport media (199E media supplemented with antibiotic-antimycotics; Sigma), and stored at −80°C until processed for virus isolation (VI ...
-
bioRxiv - Neuroscience 2021Quote: ... and the virus containing supernatant was loaded on a previously with lysis buffer pre-equilibrated 1 ml HiTrap Heparin HP column (SIGMA). Several washing steps later (increasing salt conditions ...
-
bioRxiv - Immunology 2021Quote: ... Dengue virus was added at a MOI=1 in a minimum volume of Dubelcco’s PBS with CaCl2 and antibiotics (SIGMA-Aldrich). After 2 hours ...
-
bioRxiv - Immunology 2020Quote: ... TZM-bl cells (1 X 104) were added into the mixture virus-antibody mixture containing 25 μg/ml DEAE-dextran (Sigma). The plates were further incubated for 48 h and the extent of virus neutralization was assessed by measuring relative luminescence units in a luminometer (Victor X2 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 1 x 106 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 μg per ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
-
bioRxiv - Cancer Biology 2023Quote: ... and NHA cells were transduced with equal titers of concentrated virus in complete growth media supplemented with 1 μg/ml polybrene (Sigma) for 24 hours ...
-
bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
-
bioRxiv - Biochemistry 2024Quote: ... Lentivirus was then aliquoted and stored at – 80 °C until transduction of 0.25 × 10^6 cells in 1 ml of media plus virus in 24 well plates with the addition of 8 µg/ml polybrene (Sigma) and spin inoculation for 1 h at 2,000 r.p.m ...
-
bioRxiv - Bioengineering 2019Quote: ... 1 U/ml human thrombin (EMD Millipore, Burlington, MA, USA) and 2 μg/ml ciprofloxacin (Sigma-Aldrich) ...
-
bioRxiv - Cell Biology 2021Quote: ... mouse anti-human CD9 1/1000 (clone MM2/57, Millipore), mouse anti-human CD81 1:1000 (clone TS81 ...
-
bioRxiv - Neuroscience 2020Quote: Human THP-1 cell lines were purchased from Sigma Aldrich and cultured in RPMI 1640 (Life technologies) ...
-
bioRxiv - Physiology 2022Quote: ... anti-human GCNA (1:20, catalog no. HPA023476, Sigma-Aldrich); anti-LAMIN B1 (1:500 ...
-
bioRxiv - Neuroscience 2021Quote: ... anti-human α-synuclein (syn211) (1:150,000;, 36-008, Millipore), anti-GFP (1:2000 ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... and THP-1 human monoblast cells (EMD Millipore, Billerica, MA) were used to construct the liver model for testing 14 compounds ...
-
bioRxiv - Microbiology 2020Quote: ... probed with rabbit anti-human LC3B (1:1000, Sigma L7543) and rabbit anti-human GAPDH (1:2500 ...
-
bioRxiv - Cancer Biology 2019Quote: ... and a rabbit-anti-human TCF21 antibody (Sigma; 1:250), followed by a 45-minute incubation at room temperature with a goat anti-mouse IgG HRP-linked secondary antibody (Invitrogen ...
-
bioRxiv - Bioengineering 2020Quote: ... and 2.25 μL 1 mg/mL human fibronectin (Sigma-Aldrich) was mixed and stored on ice until use ...
-
bioRxiv - Cell Biology 2021Quote: ... and blocked in 1% Human AB serum (blocking buffer, Sigma) in 1X PBS for 1 h at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse anti-human CD9 (1/1000 clone MM2/57, Millipore), rabbit anti-human 14–3-3 (1/1000 EPR6380 ...
-
bioRxiv - Cell Biology 2023Quote: ... 200 ng/ml recombinant human IGF-1 (85580C, Sigma-Aldrich), and 20 ng/ml human bFGF (PHG0021 ...
-
bioRxiv - Cell Biology 2023Quote: ... and rat anti-human E-cadherin (EMD Millipore, DECMA-1) at a concentration of 1/200 in a humidified incubator overnight ...
-
bioRxiv - Neuroscience 2023Quote: ... 10 ng mL−1 human leukemia inhibitory factor (hLIF, Millipore), 2 μM SB431542 ...
-
bioRxiv - Neuroscience 2024Quote: ... a human nuclear-specific antigen [(EMD Millipore; MAB1281 (1:1000)] ...
-
bioRxiv - Microbiology 2019Quote: ... PTP-tagged proteins were detected by incubating with anti-protein A serum (1:3000, 1 h) (Sigma, P3775) followed by Alexa Fluor® 594 goat anti-rabbit (1:250 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Virus was harvested at either 48 hours (HIV) or 72 hours (ZIKV) post-transfection by passing through 0.45 μm sterile filters (Millipore). HIV-1 stocks were prepared with pNL4-3 and the pNL4-3 deletion library ...
-
bioRxiv - Developmental Biology 2021Quote: ... were soaked overnight with human SDF-1 3.3 μg ml−1 (Sigma-Aldrich, S1577) at 4 °C ...
-
bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich, USA), and 20 ng/ mL human bFGF (Life Technologies)] with 10 μM Rho kinase inhibitor Y-27632 (Wako ...
-
bioRxiv - Neuroscience 2020Quote: ... and 1% protein phosphatase inhibitor cocktail (Sigma). Lysed tissues were incubated at 4°C for 20 min and centrifuged at 15,000 rpm for 15 min ...
-
bioRxiv - Genetics 2021Quote: ... PolyUbiquitinated proteins (1/1000, Sigma 04-262), p62 (1/1000 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The virus was propagated on VeroE6-TMPRSS2 cells using DMEM (Sigma) supplemented with 2% (v/v ...
-
bioRxiv - Bioengineering 2021Quote: ... virus was harvested and purified using an iodixanol gradient (Optiprep, Sigma). For our 7-variant pool ...
-
bioRxiv - Neuroscience 2021Quote: ... Virus or Tracer was loaded into mineral oil (Sigma-Aldrich, M3516)-filled glass pipette (WPI ...
-
bioRxiv - Microbiology 2021Quote: ... virus was concentrated using 100,000 MW cut-off Centricon concentrators (Millipore).
-
bioRxiv - Neuroscience 2021Quote: ... This concentrated virus solution was filtered (0.22 μm Millex, Merck Millipore) and stored at 4 °C until further use ...
-
bioRxiv - Cell Biology 2019Quote: ... we added concentrated virus and 5 μg/ml polybrene (Sigma-Aldrich) to MCF10A cells in growth media ...
-
bioRxiv - Immunology 2022Quote: ... virus supernatant was filtered through a 0.44 um PVDF membrane (Millipore), incubated with Lipofectamine 3000 and PLUS reagent as above ...