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Citations for Millipore Sigma :
551 - 600 of 10000+ citations for Human G protein coupled receptor 42 GPR42 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... Insulin release from islets was measured as described with Rat/Mouse Insulin ELISA kit (Millipore Sigma) (26).
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bioRxiv - Molecular Biology 2020Quote: ... Cells were routinely subjected to mycoplasma testing using the Mycoplasma PCR ELISA kit (Sigma Aldrich, 11663925910) and tested negative.
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bioRxiv - Microbiology 2021Quote: ... BCA protein assay kit (Sigma-Aldrich, USA), RNeasy kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... or Enzymatic Protein Biotinylation Kit (Sigma-Aldrich). SARS-CoV-2 RDB protein was also coupled to DyLight 650 using the DyLight® Amine-Reactive Dyes kit (Thermo Fisher scientific).
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bioRxiv - Microbiology 2021Quote: ... Protein refolding kit was purchased from Novagen. Proteins in pellet (inclusion body ...
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bioRxiv - Microbiology 2023Quote: ... the CelLytic MEM protein extraction kit (Sigma) was used as described elsewhere (López & Kolter ...
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bioRxiv - Plant Biology 2023Quote: ... Plant Total Protein Extraction Kit (Sigma-Aldrich) was used ...
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bioRxiv - Biochemistry 2024Quote: ... A Lowry assay (Sigma - total protein kit) was used to determine protein concentrations before resolving samples on an SDS-PAGE gel and transferring to nitrocellulose membrane for immunoblotting ...
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bioRxiv - Developmental Biology 2020Quote: ... equal amounts of lysates (containing 5 mg of total cellular protein from HeLa cells) were pre-cleared with protein G-agarose beads (Sigma, St Louis, MO). Pre-cleared extracts were incubated with 1 μg rat monoclonal anti-HA (Roche ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-HKU1 proteins or lacking the fusion protein (Δ Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland). HAE cultures were washed thrice with 100 μl of 1 × PBS and subsequently inoculated with S-HKU1 or VSV-G pseudoviruses ...
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bioRxiv - Developmental Biology 2020Quote: ... equal amounts of lysate (containing 5 mg of total cellular protein from HeLa cells) were pre-cleared with protein G-agarose beads (Sigma, St Louis, MO). Pre-cleared extracts were incubated with 1 μg rat monoclonal anti-HA (Roche, ...
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bioRxiv - Bioengineering 2021Quote: Protein quantification using Micro BCA protein assay (Pierce™ BCA Protein Assay Kit, Sigma Aldrich, Poole, UK) was carried out following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Membrane proteins were separated from cytosolic proteins by ultracentrifugation or a ProteoExtractR Transmembrane Protein Extraction Kit (Novagen). Protein concentration was measured with a bicinchoninic acid (BCA ...
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bioRxiv - Molecular Biology 2021Quote: ... coupled to rabbit IgG (Sigma), prepared as previously described (Oeffinger et al ...
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bioRxiv - Molecular Biology 2021Quote: ... coupled with rabbit IgG (Sigma), prepared as previously described (Oeffinger et al ...
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bioRxiv - Molecular Biology 2019Quote: ... coupled to rabbit IgG (Sigma), prepared as described (58) ...
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bioRxiv - Cell Biology 2024Quote: ... respectively) coupled to haemocyanin (Sigma). The purified antibodies were concentrated to 0.2 mg/mL and used at 1:100 dilution ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 g orange G (O3756, Sigma), and 1 g aniline blue (AB0083 ...
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bioRxiv - Genetics 2023Quote: ... 10 g NaCl 10 g (Sigma), and 1 l deionized water ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were sequentially incubated with primary and HRP-coupled secondary antibodies and proteins were detected using chemiluminescence reagents (Luminata Crescendo, Millipore) and X-ray films ...
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bioRxiv - Molecular Biology 2021Quote: ... and the activity of Rac1 and Cdc42 were assessed by affinity purification using magnetic beads coupled to the p21-binding domain of p21-activated protein kinase (PAK) (Millipore) that specifically binds the active (GTP-bound ...
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bioRxiv - Cancer Biology 2023Quote: ... the solution was filtered using a sterile syringe filter coupled to a filter unit with a low protein binding Durapore PVDF membrane (Merck Millipore) and the solution was poured into an amber tube to protect from light exposure.
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bioRxiv - Neuroscience 2021Quote: ... USA) or mouse monoclonal anti-β-actinin (42 kDa; 1:5000; Sigma Aldrich), respectively ...
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bioRxiv - Plant Biology 2019Quote: ... 0.125 M sodium phosphate monobasic) at 42°C with DNA oligo probes (Sigma) labeled with γ32P ATP ...
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bioRxiv - Developmental Biology 2020Quote: ... Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) were all purchase from Sigma.
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... was dissolved in ethanol first and subsequently preheated corn oil (42°C, Sigma) was added and strongly mixed for 1 hr in the darkness ...
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bioRxiv - Cancer Biology 2023Quote: ... derivatized for 2 hours at 42°C in 50μL of methoxyamine hydrochloride (Sigma) and 100μL N-tert-Butyldimethylsilyl-N-methyltri fluoroacetamide (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... G418 (#108321-42-2) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Zeocin (#ZEL-43-05 ...
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bioRxiv - Plant Biology 2023Quote: ... Membranes were incubated at 42 °C with PerfectHyb Plus hybridization buffer (Sigma-Aldrich) for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... cell death detection enzyme-linked immunosorbent assay PLUS (ELISA) and human anti-β-actin antibody were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Poly-ADP-ribose polymerase (PARP ...
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bioRxiv - Biophysics 2022Quote: ... Five microliters of a 5 mg mL−1 solution of protein G (Sigma-Aldrich, Tokyo, Japan) was added to the flow chamber (about 5 μL) ...
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bioRxiv - Microbiology 2020Quote: ... The immunoprecipitated antibody/chromatin complex was collected using Protein A/G magnetic beads (Millipore 16-663). The input and ChIP samples were reverse cross-linked overnight at 45°C using 0.2M final concentration of NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... 4 µg of antibodies and 20 µL of Magna Chip Protein A/G magnetic beads (Millipore) were added and samples were incubated overnight on a rotating wheel at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 uL of pre-washed magnetic beads (Magna ChIP Protein A+G Magnetic Beads, Millipore Sigma) were added to each sample for 2 hours at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Twenty-five microliters of a 50% slurry of Protein G magnetic beads (Millipore Sigma, Cat. LSKMAGG10), pre-equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... The IP samples were then incubated with 20μl of washed protein A/G–Sepharose beads (Millipore) for two hours at 4°C with constant mixing ...
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bioRxiv - Cancer Biology 2021Quote: ... The total cell lysates were precleared via incubation with protein G-linked agarose beads (Sigma; #1124323300) for 2 hours at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The immune complexes were collected by incubation (2 h, 4°C) with protein G-agarose (Sigma). Co-IP assays were performed by using Pierce™ Protein G Agarose (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitation was carried out with β-catenin monoclonal antibody and Protein G magnetic beads (Sigma-Aldrich). For PCR ...
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bioRxiv - Cell Biology 2022Quote: ... followed by an overnight incubation with Pure-Proteome Protein A/G mix magnetic beads (Millipore Sigma). Samples were washed as indicated by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by a 4 h of incubation with protein A/G agarose beads (LOT: 3460992, Millipore). The purified DNA fragments bound by ATF3 were analyzed using qPCR ...
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bioRxiv - Molecular Biology 2023Quote: ... 60ul of protein G Sepharose beads conjugated to M2 Flag antibody (Millipore Sigma Cat. M8823-5ML) was added to each sample ...
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bioRxiv - Microbiology 2023Quote: ... The flow-through was then applied to a Protein G Sepharose (Millipore Sigma, #GE17-0618-01) containing column (5ml polypropylene columns ...
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bioRxiv - Genomics 2023Quote: ... Samples were added to Magna ChIP™ Protein A+G Magnetic Beads (16-663, Sigma-Aldrich) precoated with specific antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 uL of pre-washed magnetic beads (Magna ChIP Protein A+G Magnetic Beads, Millipore Sigma) were added to each sample for 2 hours at 4º C ...
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bioRxiv - Cancer Biology 2022Quote: ... 50µL of protein G beads were coated 2ug of anti-AGO2 antibody (Sigma SAB4200085 Lot# 0000089486) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... DNA was then added to MagnaChip magnetic protein A/G beads (16-663, Millipore, Burlington, USA) overnight that were incubated with either 5-hmC ...
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bioRxiv - Molecular Biology 2023Quote: ... The purification of monoclonal antibodies was performed by affinity chromatography using Hitrap Protein G Column (Sigma) starting from 150 ml of hybridoma cell supernatant ...
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bioRxiv - Microbiology 2023Quote: ... incubated with mouse anti-HA mAb clone 12CA5 conjugated with protein G agarose beads (Sigma-Aldrich) for 16 h at 4°C ...
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bioRxiv - Immunology 2023Quote: ... anti-Myc agarose affinity beads (A7470) and protein A/G agarose affinity beads (P6486/E3403) (Sigma); mouse recombinant M-CSF (315–02 ...