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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for Human ACE2 18 615 Recombinant Protein Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... coli BL21(DE3) as a fusion protein containing an N- terminal His6-tag (Novagen) and purified by metal-affinity chromatography (Ni-NTA ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Biophysics 2021Quote: ... Plasminogen (human plasma purified protein, Sigma-Aldrich, Germany) and Fibrinogen (human plasma purified protein ...
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bioRxiv - Biophysics 2021Quote: ... and Fibrinogen (human plasma purified protein, Sigma-Aldrich). Experimental conditions are presented in the table I.
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bioRxiv - Biophysics 2021Quote: Plasminohen (human plasma purified protein, Sigma-Aldric, Germany)
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bioRxiv - Developmental Biology 2019Quote: ... 200nL 0.1µg/µL human noggin protein (Sigma H6416) was injected into the blastocoel of embryos at stage 8 ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Microbiology 2023Quote: ... stably expressing human ACE2 and TMPRSS2 (ref.42, 43) were maintained in Dulbecco’s modified Eagle’s medium (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... HEK 293TACE2 and A549ACE2 cells were generated by a transduction with a lentivirus expressing human ACE2 (kind gift from Benjamin Murrel, Karolinska Institutet) in the presence of 6 μg/ml Polybrene (Sigma-Aldrich, H9268). Clones were isolated and selected for homogeneous ACE2 expression as determined by HTM ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated with FITC-conjugated goat anti-human IgG (Fc specific) (Sigma St-Louis, MI) or FITC-conjugated goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: Human cells (HeLaK and Hek293T) were cultured in DMEM (Biowest) supplemented with 10% FCS (Sigma-Aldrich) at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: Organotypic entorhino-hippocampal tissue cultures (≥ 18 days in vitro) were treated with mouse recombinant Tumor Necrosis Factor α (60 pM or 6nM; Sigma, Cat# T7539) for 24h ...
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bioRxiv - Microbiology 2019Quote: The genes of either E protein or E DIII protein (residues 301-403) of ZIKV (GZ01, KU820898) without tag were cloned into pET28a vectors (Novagen) and expressed by IPTG-induction in BL21 (RIL ...
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bioRxiv - Biophysics 2021Quote: ... The GB1 tag was cleaved from the oxidized proteins by incubating overnight with thrombin (10 U.mg−1 protein, Sigma-Aldrich). The cleaved protein was purified by reversed phase high-performance liquid chromatography (RP-HPLC ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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bioRxiv - Genetics 2019Quote: Recombinant proteins were expressed in Escherichia coli BL21(DE3)pLys using pET28a+ (Novagen) and purified using Ni-Sepharose affinity medium (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant proteins were produced and purified on glutathione-agarose column (G4510, Sigma) for GST-tagged protein or on TALON-Cobalt beads column (635507 ...
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bioRxiv - Plant Biology 2023Quote: ... The over-expressed recombinant proteins were then coupled to CNBr-activated Sepharose (Sigma) at a ratio of 2.5 mg protein to 1 ml Sepharose slurry as described by the manufacturers.
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bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples for mass spectrometry were purified and concentrated using ZipTipC-18 (Merck, Millipore, Darmstadt, Germany).
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bioRxiv - Neuroscience 2021Quote: ... irradiated 18% protein chow (Teklad Global) supplemented with 0.2% w/w bis(cyclohexanone) oxaldihydrazone (“cuprizone,” Sigma). Control mice received only the milled chow ...
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bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
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bioRxiv - Microbiology 2023Quote: ... strep tag and myc tag were obtained from Sigma, IBA and Abcam ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Chinese hamster ovary (CHO) cells (Sigma, passage 6 to 20) were chosen for this study due to the absence of endogenous ASIC-like currents [53] and were grown using standard procedures in the following medium ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... CHO-K1 cells were cultured in DMEM/F12 (Sigma-Aldrich) with 9% FBS (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant ephrins or Fc were preclustered in a 5:1 molar ratio with either mouse or goat anti-human Fc (Millipore Sigma cat. no. 16760 and 12136 respectively) for 30’ at 37°C.
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bioRxiv - Immunology 2020Quote: NTD-Fc or RBD-Fc proteins were diluted in sterile PBS and formulated as an emulsion with TiterMax Gold adjuvant (Sigma-Aldrich, St. Louis, MO) to a final antigen concentration of 250 µg/mL ...
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bioRxiv - Biophysics 2019Quote: ... cytokeratin 18 (Millipore, MAB3234) 1:200 ...
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Myofiber injury induces capillary disruption and regeneration of disorganized microvascular networksbioRxiv - Physiology 2021Quote: ... and 18 NaHCO3 (Sigma) equilibrated with 5% CO2/95% N2 ...
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bioRxiv - Cell Biology 2022Quote: ... Peptide-18 (Merck Millipore) was recovered in water at a stock concentration of 1 mg/ml and microinjected in the oocyte cytoplasm.
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bioRxiv - Physiology 2022Quote: ... and 18 NaHCO3 (Sigma) equilibrated with 5% CO2/95% N2 ...
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bioRxiv - Cell Biology 2021Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35 days ...
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bioRxiv - Microbiology 2020Quote: ... were treated with or without 25-100 U/mL of human recombinant IFN-α (Sigma-Aldrich) for 4-12 hours after which total RNA was isolated and subjected to qRT-PCR in order to measure expression of ISGs.
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bioRxiv - Microbiology 2019Quote: ... EVs were incubated with Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) at different final concentration (0 to 10 µg/ml) ...
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bioRxiv - Microbiology 2021Quote: For expression of C13/N15 labeled recombinant human wildtype-MIF BL21-(DE3)-Rosetta-pLysS bacteria (Novagen) were transformed with pET11a-MIF and spread on LB-Agar plates containing 50 µg/mL carbenicillin and 34 µg/mL chloramphenicol ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 µg/ml BSA fraction V and 10 µg/ml human recombinant in sulin (both Sigma)) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant human EGF (Cat# E9644) and Glucose Quantification Kit (Cat# GAGO-20) purchased from Sigma-Aldrich; Cisplatin (Cat# 232120 ...
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bioRxiv - Microbiology 2019Quote: ... Glutamax and Pen/Strep and with 100 U/mL recombinant human IL-2 (Roche; Sigma # 10799068001). For the analysis of reverse transcription products ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1: 85580C, Sigma-Aldrich, MO, USA), and 20 ng/mL human bFGF] for 35±3 days25 ...
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bioRxiv - Physiology 2023Quote: ... and recombinant human interleukin-1β (IL-β) were obtained from Millipore Sigma (St Louis, MO, USA). Thapsigargin (TG ...
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bioRxiv - Molecular Biology 2023Quote: ... TGFB1 signaling was induced with 5 ng/ml final concentration of human recombinant TGFB1 (Sigma-Aldrich) for 4 h.
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bioRxiv - Microbiology 2020Quote: ... cells were stained with 1/200 diluted Goat Anti human IgG Fc-FITC antibody (Cat: F9512, Sigma) for 45 min and analyzed using flow cytometry (CytoFLEX ...
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bioRxiv - Molecular Biology 2020Quote: ... 10% FCS and human fibroblast growth factor-2 (5 ng/mL, Sigma-Aldrich, St Louis, MO, USA) at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: ... and binding was detected with an anti-human IgG (Fc specific) (1:5000 dilution, Sigma-Aldrich, A0170) or anti-FLAG (1:1000 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... Antibodies and dyes used in this study were: Anti-Human IgG Fc specific (Millipore Sigma I2136-1ML), Anti-6X His tag (Abcam ab9108) ...
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bioRxiv - Bioengineering 2020Quote: ... or human hemoglobin (all proteins were purchased from Sigma). Protein nanorods were prepared using chemically modified human serum albumin.
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bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
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bioRxiv - Immunology 2020Quote: ... FCS (Sigma Life Science ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The recombinant protein was then concentrated using centrifugal filter units (Amicon Ultra-4, Millipore) and dialyzed using Slide-A-Lyzer dialysis cassette into storage buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat ...