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Citations for Millipore Sigma :
51 - 100 of 1532 citations for Hantaan Virus Nucleoprotein HEK293 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
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bioRxiv - Microbiology 2021Quote: ... nucleoprotein of OC-43 (1:1000, Sigma MAB9013); HCoV-NL63 ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tag was cleaved by overnight incubation with thrombin (from bovine plasma, Sigma Aldrich) at 4 °C during dialysis ...
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bioRxiv - Immunology 2022Quote: ... and 100 µL of alkaline phosphatase-conjugated anti-HIS tag antibody (Sigma-Aldrich, cat: A5588), diluted 1:2,000 in TBST ...
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bioRxiv - Bioengineering 2021Quote: ... then probed with the conjugated anti-6x His tag® mouse monoclonal antibody (Sigma-Aldrich) overnight at 4ºC or 1 hour at room temperature and rewashed three times with washing buffer (CMT ...
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bioRxiv - Physiology 2020Quote: ... the N-terminal-His-tag was labeled with the Ni-NTA Atto550 fluorophore (Sigma-Aldrich) following the protocol provided by the manufacturer ...
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bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
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bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
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bioRxiv - Biophysics 2021Quote: ... followed by cleavage of the His-tag with 3C protease and dephosphorylation with bovine alkaline phosphatase (Sigma). Further purification was performed by ion exchange chromatography (Source Q for WT and the S897E ...
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bioRxiv - Cell Biology 2019Quote: ... The His-tag was removed by cleavage with 16 U of thrombin (Ref 27-0846-01, Sigma) added directly onto the beads for 2h at 25°C ...
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bioRxiv - Biochemistry 2021Quote: ... pneumoniae LicB with an N-terminal 10×His affinity tag in a modified pET-19b vector (Novagen) was overexpressed in E ...
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bioRxiv - Immunology 2022Quote: ... the 6x HIS tag region of the recombinant protein was removed following the vector manufacturers specifications (Novagen). This recombinant SmCI-1 was used to generate a rabbit anti-Sm-CI-1 polyclonal antibody using the Custom pAb service offered by Genscript.
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bioRxiv - Molecular Biology 2020Quote: ... mouse-anti-IAV nucleoprotein (Millipore: MAB8251; RRID: AB_95293; 1:1,000), anti-BVDV polyclonal antibody B224 (kindly provided by Kenny Brock ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells stably expressing CCR5 were labeled with 2ug/ml rabbit anti-Flag tag antibody (F7425, Sigma, St. Louis, MO, USA) in FACS buffer (PBS with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Microbiology 2021Quote: ... Dialysis was performed in concert with cleavage of the His-SUMO tag using the SUMO protease (Sigma Aldrich) at 20U/1mg of protein ...
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bioRxiv - Biochemistry 2020Quote: ... 2020) genes fused with an N-terminal His-tag sequence have been inserted into a pET28a vector (Novagen) for protein expression in E ...
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bioRxiv - Cell Biology 2023Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was flown through 500 μL of Roche cOmplete™ His-Tag purification resin (Millipore Sigma, #5893682001) for His-tag tagged proteins ...
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bioRxiv - Molecular Biology 2021Quote: ... elution profiles and sample purity were confirmed by Western blot with antibodies against His tag (Sigma, H1029, lot 033m4785) and Strep tag (IBA ...
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bioRxiv - Molecular Biology 2020Quote: ... the His6-tag-fusion proteins were purified from the supernatant by a HIS-Select® Nickel Affinity Gel (Sigma) column ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Biophysics 2021Quote: ... The his-tag affinity purified stator was concentrated to 1 ml using a 100 K MWCO Amicon device (Millipore). The samples were subjected to size exclusion chromatography using Enrich SEC650 column (Bio rad ...
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bioRxiv - Immunology 2021Quote: ... Efficiency of the expression and purification of recombinant proteins were determined by 15% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:10,000) (Sigma). Protein concentrations were quantified by comparison with a curve of BSA resolved by SDS-PAGE.
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bioRxiv - Microbiology 2022Quote: ... Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3) cells (Novagen). Protein expression was induced during logarithmic growth through the addition of 0.5mM isopropyl-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2023Quote: All recombinantly produced proteins were enriched via affinity chromatography using Roche cOmplete™ His-Tag Purification Resin (Millipore-Sigma) and chromatography on an AKTA Start (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: All recombinantly produced proteins were enriched via affinity chromatography using Roche cOmplete™ His-Tag Purification Resin (Millipore-Sigma) and chromatography on an AKTA Start (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were harvested and Fab fragment was purified via the heavy chain His tag using affinity chromatography with Talon resin as per the manufacturer’s specifications (Sigma). Purified protein samples were dialysed into PBS as quantified by absorbance at 280nm.
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Biochemistry 2021Quote: HEK293 cells (Sigma:85120602) were cultured using DMEM (Gibco™ ...
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bioRxiv - Biophysics 2023Quote: HEK293 cells (Sigma Aldrich) and were cultured in DMEM (Gibco ...
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bioRxiv - Biochemistry 2020Quote: Full length human KBP residues 1-621 in a PSTCm1 expression vector (with kanamycin resistance and a N-terminal thrombin cleavable 6 x His-tag) was expressed in Rosetta2 cells (Novagen). Following immobilised metal-affinity chromatography with Ni-NTA resin (Qiagen) ...
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bioRxiv - Evolutionary Biology 2020Quote: Proteins were expressed in a pET28/30 vector containing an N-terminal His tag with a TEV protease cleavage site (Millipore). For each protein ...
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bioRxiv - Cell Biology 2020Quote: ... the α-PheRS or α-PheRSCys mutant cDNAs were cloned with His tags at the N-terminal end into the pET-28a plasmid expression vector (Novagen). Wild-type β-PheRS cDNAs were cloned into the pET LIC (2A-T ...
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bioRxiv - Biophysics 2021Quote: The bacteriophage T7 gp16 gene was cloned into the pET-16b vector (ampr and 10x-His tag) from Novagen (Germany), inserted between NdeI and XhoI restriction sites ...
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bioRxiv - Biochemistry 2021Quote: SUMO-peptide fusions were cloned into a pDW363 vector that appends a BAP sequence and 6x-His tag to the N-terminus of the protein and transformed into Rosetta2(DE3) cells (Novagen). Rosetta2(DE3 ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA sequence encoding 1053-1264 aa mouse was amplified and cloned upstream of sequences encoding a polyhistidine stretch (His) tag in pET23b (Novagen). The mGli2-His recombinant protein was expressed in BL21 (DE3 ...
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bioRxiv - Plant Biology 2020Quote: ... the coding sequence was excised with BamHI/EcoRI and ligated downstream of the His tag into the multiple cloning site MCS1 of pETDuet-1 (ampicillin resistance; Novagen), yielding Sll0171-pETDuet-1 ...
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bioRxiv - Bioengineering 2022Quote: ... It was cloned in the expression vector pET30 Ek/lic containing an N-terminal His-tag with the help of the ligation independent Ek/lic system according to the protocol proposed by Novagen, UK ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... was cloned into the protein expression vector pET28a (+) containing an IPTG-inducible promoter and a C-terminal 6x-His tag (Novagen). Expression of CosR-His was then induced in E ...
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bioRxiv - Microbiology 2019Quote: ... and the PCR product was purified and cloned in-frame with a His-tag into the IPTG-inducible expression plasmid pET-15b (Novagen) to create construct pET15b-Pnf ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mouse anti-FLAG M2 monoclonal antibody (F1804) and mouse anti-His-tag monoclonal antibody (D291-3S) were purchased from Sigma□Aldrich (St ...
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bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
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bioRxiv - Synthetic Biology 2022Quote: ... Grids were blocked in 2% BSA in TBST for 30 minutes and then moved into a 20 µl drop of anti-His tag primary antibody (Sigma) diluted 1:100 ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...