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Citations for Millipore Sigma :
501 - 550 of 820 citations for HVEM Rhesus Macaque HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... MaxiSorp plates (Thermo) were coated with 100µl of goat anti-human Fc antibody (Sigma) diluted 1:500 in PBS overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed before detection and goat anti-human Fc-HRP conjugated antibody (Sigma) (1:5000 dilution in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... 17 div cultured neurons were incubated with TrkB-Fc (1 µg/mL, T8694-Sigma) a BDNF scavenger suppressing BDNF binding to TrkB (Sakuragi et al ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% (vol/vol) FCS and 100 U/mL penicillin/streptomycin (Sigma Aldrich). D2.0R-GFP and 4T1-mCherry cells were generated by lentiviral transduction with TurboGFP and mCherry reporter ...
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bioRxiv - Cancer Biology 2022Quote: ... in RPMI-1640 medium complete (R8758, Sigma-Aldrich, supplemented with: 10% FCS (Sigma-Aldrich), 2mM L-glutamine (Biochrom) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 10% heat inactivated Fetal Calf Serum (FCS) (Sigma-Aldrich, St. Louis, Missouri, USA) and under standard culture conditions in humidified incubator at 37°C with 5% CO2.
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bioRxiv - Neuroscience 2020Quote: MaxiSorp plates were coated with 100 µl of goat anti-human Fc antibody (Sigma) diluted 1:500 in PBS or 3 mg/ml of an anti-VNAR antibody (courtesy of Martin Flajnik ...
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bioRxiv - Cancer Biology 2021Quote: ... consisting of 1% fetal calf serum (FCS; #S0615, Sigma, part of Merck, Darmstadt, Germany) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... binding was detected by using goat-anti-hIgG(Fc)-HRP (1:70000, A0170, Sigma). Binding was visualized by using TMB substrate ...
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bioRxiv - Microbiology 2022Quote: ... RPMI-1640 or MEM media and 10% Fetal Calf Serum (FCS, Sigma cat. F7524). THP-1 cells media was also supplemented with 0.05 mM β-mercaptoethanol (Euromedex ...
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bioRxiv - Microbiology 2021Quote: ... Cell permeabilization and blocking was performed in IF buffer (5% FCS, 0.05% saponin (Sigma) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... and at day 13 with 50 μg of RBD-Fc plus Titermax adjuvant (Sigma). Blood were collected from individual mice one week after the last immunization (day 20 ...
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bioRxiv - Immunology 2021Quote: ... The cultures were supplemented on day 2 with FCS (10% v/v; Sigma-Aldrich). Medium was replaced every 3 days thereafter with fresh R+ containing FCS (10% v/v ...
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bioRxiv - Immunology 2020Quote: Cells were thawed and subsequently washed in IMDM+10%FCS+75U/ml DNAse (Sigma). To minimize day-to-day variation ...
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bioRxiv - Cancer Biology 2022Quote: ... 293T and NIH-3T3 cells were grown in DMEM with 10 % FCS (Sigma-Aldrich, St ...
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bioRxiv - Physiology 2023Quote: Rm-EphrinA4/Fc chimera (R&D) was clustered with anti-human Cy3 antibody (Sigma) diluted 1 ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% Fetal Calf Serum (FCS, Capricorn Scientific) and 0.5% Neomycin (Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: ... 10 μg/mL goat anti-human IgG1 Fc F(ab’)2 (Sigma-Aldrich, I3391) was coated in 96-well NUNC Immunoplate (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Later the brain was transferred to a tube containing fluorinert (FC-40, Sigma-Aldrich) to reduce the background signal ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in a wash solution of 10% fetal calf serum (FCS, Sigma-Aldrich) in DMEM (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS) (Sigma Aldrich, F7524, St. Louis, MO, USA). Cytarabine-resistant cell lines were generated as described previously by us [15] ...
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bioRxiv - Developmental Biology 2023Quote: ... medium changed to IMDM+10%FCS+Penicillin-streptomycin + 8-bromo-cAMP (10μM, Sigma-Aldrich), and then incubated overnight at 37°C 5%CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... after which the cells were resuspended in PBS supplemented with 1% FCS (S7524, Sigma) and 2mM EDTA and kept at 4°C until the plate was measured in the flow cytometer ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant supplemented with 1% Pen/Strep and 10% FCS (Sigma-Aldrich, Missouri, USA) and stored in the dark at 4 °C.
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bioRxiv - Cancer Biology 2021Quote: Human embryonic kidney (HEK) 293T cells and human non-small-cell lung cancer (NSCLC) A549 cells were cultured in DMEM (Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: Lentiviral particles encoding guide RNA and Cas9 were produced in HEK 293T cells using mouse Irf7 CRISPR lentiviral transfer construct targeting exon 7 (target ID #MM0000202587) of Irf7 (Sigma Aldrich). Lenti CRISPR universal non-target plasmid was used as a control (Sigma Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... and HEK-293T-ACE2-DPP4-TMPRSS2 were maintained by supplementing the culture medium with 1 μg/ml Puromycin (Sigma-Aldrich, USA), and 1.5 μg/ml Blasticidin (InvivoGen ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK FRT host cells harboring the Flp-In™ T-REx system were maintained in culture medium (DMEM-high glucose (Sigma) medium ...
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bioRxiv - Immunology 2021Quote: ... pTT3-HKU1 or pTT3-OC43 Spike were transfected into suspension-adapted 293T cells using 293 Free transfection reagent (EMD Millipore Cat# 72181) or PEI transfection reagent (PolySciences Inc ...
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bioRxiv - Genetics 2020Quote: Wildtype and mutant COCH-expressing FLp-inTM T-RExTM 293 cells were cultured in DMEM-AQ medium (Sigma Aldrich, Saint Louis, USA) supplemented with 10% Fetal Calf Serum ...
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bioRxiv - Microbiology 2020Quote: ... or 293CD4 cells (293 cells constitutively expressing human CD4) were grown in Dulbecco’s modified Eagle’s medium (DMEM, Sigma, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 10% FCS (PAN-Biotech, P40-37500) and 1% penicillin/streptomycin (Sigma, P0781-20ML). Medium was changed every three days until adult microglia were treated as indicated after 8 days in vitro (DIV).
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 10% FCS (PAN-Biotech, P40-37500) and 1% penicillin/streptomycin (Sigma, P0781-20ML) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed with FACS buffer (PBS, 2%FCS) and fixed in 4% Paraformaldehyde (Sigma) in PBS for 10 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were cultured in MEM-Alpha (Biological Industries) supplemented with 10% Heat Inactivated FCS (Sigma), 50IU rhIL-2 (Peprotech ...
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bioRxiv - Molecular Biology 2020Quote: ... and anti-Human IgG (Fc specific) −Peroxidase antibody produced in goat was from Sigma-Aldrich. Two TNFα-antagonists were purchased ...
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bioRxiv - Molecular Biology 2022Quote: ... The media were supplemented with 10% FCS and 1% penicillin-streptomycin (Sigma-Aldrich, Taufkirchen, Germany). Cells were maintained at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Immunology 2020Quote: ... 200 μl MTT mix (DMEM supplemented with 10% FCS containing 250μg/ml tetrazolium bromide, Sigma) was added to each well ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 10% FCS (PAN-Biotech, P40-37500) and 1% penicillin/streptomycin (Sigma, P0781-20ML). Medium was changed every three days until adult microglia were treated as indicated after 8 days in vitro (DIV).
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 10% FCS (PAN-Biotech, P40-37500) and 1% penicillin/streptomycin (Sigma, P0781-20ML) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... SMGs were digested in 500μl RPMI-1640 containing 5% fetal calf serum (FCS; Sigma Aldrich), 6µl Collagenase-II (23mg/mL ...
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bioRxiv - Biophysics 2019Quote: ... with the addition of 10 mM HEPES and 0.5% fetal calf serum (FCS, Sigma-Aldrich) was used if not stated otherwise ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% fetal calf serum (FCS, Biowest) and 1% Penicillin–Streptomycin (PS, Sigma-Aldrich) at 37 °C and 5% CO2 in a humidified incubator.
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bioRxiv - Cell Biology 2020Quote: ... and re-suspended in ice cold 2 % (v/v) FCS/PBS (PAA Laboratories/Sigma Aldrich) following centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in flow cytometry buffer (FB: 2% FCS, 3 nM EDTA (Sigma, E9884) in PBS) ...
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bioRxiv - Immunology 2021Quote: ... 107 BAL cells per mL were suspended in 90% FCS and 10% DMSO (Sigma-Aldrich) and cryopreserved in liquid nitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μL of goat anti-human IgG (Fc specific)-Peroxidase antibody (1 : 5000 dilution, Sigma) was added and incubated for another 1 h at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were differentiated into macrophages in complete media (RPMI, Gibco + FCS, Gibco +1%Penstrep, Sigma) containing 5% FCS and additional 100ng/mL M-CSF (PeproTech ...
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bioRxiv - Biophysics 2023Quote: ... The anti-human Fc capturing mAb (GG-7) was from Sigma-Aldrich (St. Louis, MO). LIBS-2 was purchased from EMD Millipore (Billerica ...
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bioRxiv - Cancer Biology 2022Quote: ... MDA-MB-231 and BrM cells were cultured in 10% (v/v) FCS (Sigma-Aldrich)-supplemented RPMI-1640 (Sigma-Aldrich ...