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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: HEK293 cells were transfected at 80–90% confluency using PEI transfection reagent (Sigma) with 2 mg/ml PEI per mg of DNA ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells were transfected with plasmids using polyethylenimine according to manufacturer’s instructions (Sigma). Neurons were transfected by the Ca2+-phosphate method (53) ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells were seeded on glass coverslips coated with poly-L-ornithine (Sigma) in clear 12-well plates (3 × 105 cells/well ...
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bioRxiv - Cell Biology 2022Quote: HEK293 Flp-In T-REx stable lines were cultured in DMEM (Sigma, D5671) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were transfected with lentiviruses particles containing Pin1 shRNA (Sigma-Aldrich, TRCN0000010577) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293 cells were transfected using Polyethylenimine (PEI,Sigma Aldrich; St. Louis, Missouri, USA). PEI and vector media were combined in a ratio of 5µL PEI and 0.5µg vector in 250µL of serum-free DMEM and incubated for 10 min at room temperature ...
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bioRxiv - Genetics 2019Quote: ... HEK293 cells were cultured in high glucose Dulbecco’s modified Eagle’s medium (DMEM; Sigma) supplemented with 10% fetal bovine serum at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: HEK293 or 293T cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) supplemented with 10% fetal calf serum ...
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bioRxiv - Microbiology 2022Quote: ... and HEK293 cells and were grown in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma) supplemented with 10% v/v foetal calf serum (FCS ...
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bioRxiv - Microbiology 2023Quote: HEK293 T cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Sigma, D6429) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals ...
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bioRxiv - Developmental Biology 2024Quote: HEK293 cells were seeded onto glass coverslips coated with Poly-L-Lysine (Sigma) and left to adhere overnight prior to transfection with plasmid DNA ...
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bioRxiv - Neuroscience 2020Quote: ... or mHTT protein (1-82aa, Millipore, MAB5492, 1:500), and the appropriate biotinylated goat anti rabbit or goat anti mouse or secondary antibody (Vector Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Developmental Biology 2019Quote: ... They were incubated at 28.5°C in 0.3% Danieau medium supplemented 5h after birth with 0.003% (wt/vol) 1-phenyl-2-thiourea (PTU) (Sigma Aldrich) to inhibit pigment formation ...
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bioRxiv - Bioengineering 2020Quote: ... The microparticles were hydrated for at least 24 hours in a 1% (w/v) solution of GelMa and 0.05 wt% Lithium phenyl- 2,4,6-trimethylbenzoylphosphinate (LAP Sigma- Aldrich, 900889) in either PBS ...
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bioRxiv - Microbiology 2020Quote: ... 1 wt% melted agarose with ultra-low gelling temperature (Type IX-A, A2576, Sigma-Aldrich, Saint Louis, MO, USA) and cells ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 were stained with an anti-His antibody (1:5,000) and secondary antibody conjugated with fluorescein (Sigma; F0257; 1:5,000). The solution was then centrifuged at 12,000 g for 5 min ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Molecular Biology 2019Quote: ... and zinc binding proteins were eluted according to the supplied protocol using HIS-Select wash and elution buffers purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Cell Biology 2020Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich), and 20 ng/mL human bFGF (Life Technologies)] for 14 to 35 days ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human vinculin (mouse monoclonal, VIN-1, Sigma Aldrich, V9131; WB 1:1000), anti-human Hic-5 (mouse monoclonal ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich), and 20 ng/mL human bFGF (Life Technologies)] for 14 to 35 days.
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bioRxiv - Physiology 2023Quote: ... glucose tolerance was assessed via intraperitoneal (i.p.) injection of glucose (2 g/kg, 20% wt/vol D-glucose [Sigma] in 0.9% wt/vol saline) in 4-hour fasted mice ...
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bioRxiv - Immunology 2023Quote: ... the binding control anti-HLA class I (clone W6/32, 10 mg/ml Sigma, St. Louis, MO), an anti-human MHC class II (HLA DR ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were digested by trypsin/lysC (protein: enzyme = 25:1, Millipore) at 37°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... membrane-extracted proteins were incubated for 1.5 hours at 4°C with purified human IgG (Sigma) that had been previously coupled to CNBr-activated Sepharose beads (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant lyophilized proteins were resuspended using 0.1% (w/v) human serum albumin (HSA) (A9731, Sigma-Aldrich) in sterile water ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5% (wt/vol) Brilliant Blue FCF dye solution (Sigma-Aldrich) was prepared in 5% sucrose solution ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 3% (wt/vol) skim milk powder (Sigma®). SDS-PAGE gels were stained with GelCode® Blue Staining Reagent (Thermo Scientific ...
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bioRxiv - Biophysics 2022Quote: ... N-methylene-bis-acrylamide (BIS, 2% wt/vol, M1533, Sigma) were mixed with PBS to obtain ...
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bioRxiv - Physiology 2021Quote: Wildtype (WT) and SWELL1 knockout (KO) 3T3-F442A (Sigma-Aldrich) cells were cultured and differentiated as described previously24 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in WT medium (DMEM high-glucose [Sigma] ...
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bioRxiv - Bioengineering 2022Quote: ... the 6 wt.% gelatin solution (Porcine skin, Type A, Sigma) in deionized (DI ...
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bioRxiv - Neuroscience 2023Quote: ... dissolved in 0.45% (wt/vol) hydroxypropyl-ß-cyclodextrin (Sigma-Aldrich) during 10 weeks as previously reported [37,40,41] ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.3 wt% lead(II) acetate trihydrate (215902, Sigma-Aldrich) was used ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.2 wt% of N,N′-methylenebis(acrylamide) (Sigma-Aldrich) was dissolved in deionized water ...
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bioRxiv - Pathology 2023Quote: ... in saturated picric acid (1.3% wt/vol; P6744, Sigma-Aldrich), for 60 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: WT or mutant EqtII cDNA was subcloned into pET45b (+) (Novagen), and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3 ...
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bioRxiv - Cell Biology 2024Quote: ... WT cells were grown with “heavy” [13C615N2]-L-lysine (Sigma) at a final concentration of 20 mg/L and the tul1Δ and vps4Δ mutant cells were grown with “light” [12C614N2]-L-lysine at a final concentration of 20 mg/L ...
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bioRxiv - Microbiology 2024Quote: ... 0.02% (wt/vol) sodium azide using an Amicon concentrator (Millipore). The NRP2-Fc was purified using a Protein-A affinity column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2024Quote: ... and 0.1% (wt/vol) fatty acid-free BSA (Sigma, #A6003)) ...
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bioRxiv - Cell Biology 2023Quote: ... cholesterol was delivered by incubating cells for 1 h with 100 μM of water-soluble methyl-β-cyclodextrin–cholesterol complex (Sigma-Aldrich) in DMEM/F-12 ...
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bioRxiv - Cell Biology 2022Quote: ... HCP-1 was produced similarly but in Hi-5 cells maintained in EX-Cell 405 medium (Sigma-Aldrich) and collected after 66h infection ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with anti-His primary antibody 1:5000 in 4% milk in PBS-T (Sigma) for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of Cypridina noctiluca luciferase and Omicron RBD or its mutants were co-transfected (9:1) into HEK293 cells using X-tremeGENE 9 (Millipore-Sigma XTG9-RO). After 16 hours ...
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bioRxiv - Genetics 2021Quote: ... Anti-His (SCBT, #sc-8036; used in 1:2000), anti-GST (SCBT, #459; used in 1:5000) and anti-Flag (Sigma-Aldrich, #F7425;used in 1:2000). Peroxidase conjugated anti-mouse (Jackson Immunoresearch ...
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bioRxiv - Bioengineering 2019Quote: ... 1 U/ml human thrombin (EMD Millipore, Burlington, MA, USA) and 2 μg/ml ciprofloxacin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-human CD9 1/1000 (clone MM2/57, Millipore), mouse anti-human CD81 1:1000 (clone TS81 ...