Labshake search
Citations for Millipore Sigma :
551 - 600 of 10000+ citations for HLA A*0201 HPV16 E7 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... SI and protein extracts from human and mouse samples were resolved in SDS-PAGE transferred to Immobilon-P PVDF membranes (Millipore). Membranes were activated with methanol and blocked with 4 % bovine serum albumin (BSA ...
-
bioRxiv - Cell Biology 2023Quote: ... Cytokine analysis was performed by Cincinnati Children’s Hospital Research Flow Cytometry Core per kit instructions (Milliplex MAP human protein panel, Millipore-Sigma).
-
bioRxiv - Biochemistry 2023Quote: ... 1:200 dilution of human C-reactive protein (MPBIO) including additional 5 mM CaCl2 or 1:1000 anti-HRP from rabbit (Sigma) in blocking buffer ...
-
bioRxiv - Biochemistry 2024Quote: ... The protein expression of the mouse or human 5-HT3A was induced by the addition of 4 μg/mL tetracycline (Sigma). Valproic acid (Sigma ...
-
bioRxiv - Biochemistry 2022Quote: ... as a His-tag fusion and Ars2206-531 into pRSFduet vector (Novagen) as a Strep-tag fusion ...
-
bioRxiv - Molecular Biology 2019Quote: ... Monoclonal anti-His and anti-Flag antibodies were obtained from Sigma Aldrich, St ...
-
bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Buffer G and allowed to go overnight at 4 °C ...
-
bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Lysis/Binding Buffer (50 mM Na2HPO4 ...
-
bioRxiv - Microbiology 2020Quote: ... 10 % heat-inactivated fetal bovine serum (HI-FBS) (v/v) (Sigma-Aldrich), and 1 % antibiotic-antimycotic (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2020Quote: ... The supernatant was loaded onto His-Select Nickel Affinity Gel (Sigma-Aldrich) equilibrated with buffer A (25 mM Tris–HCl ...
-
bioRxiv - Cell Biology 2021Quote: ... The β-Spectrin-His construct was transformed into BL21(DE3) cells (Novagen) and induced for protein expression with 0.5mM IPTG (Invitrogen ...
-
bioRxiv - Immunology 2022Quote: 30µL/well of rabbit anti-His tag antibody (Sigma Aldrich, catalog #SAB5600227) was adsorbed at a concentration of 0.5 μg/mL on to 384 well microplates (Thermofisher ...
-
bioRxiv - Bioengineering 2022Quote: ... Pre-cleared supernatant was mixed with Ni-NTA His-Bind Resin (Novagen) (Pajenda et al. ...
-
bioRxiv - Immunology 2020Quote: ... supplemented with 15% FBS HI (Life Tehnologies) + 1% Pen/Strept (Sigma-Aldrich), 1% L-glutamine (Life Technologies) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Purification of rcCBP2 was performed by HIS-Select HF Nickel Affinity (Sigma) and gel permeation chromatography on Superdex 75 column (GE Healthcare Life Sciences) ...
-
bioRxiv - Cell Biology 2019Quote: ... for 10 minutes followed by anti-his antibody (Sigma, Cat. No. 11922416001) for another 10 mins ...
-
bioRxiv - Microbiology 2021Quote: ... The crude lysate rocked with HIS-select nickel affinity gel resin (Sigma) for 45 min at 4 °C ...
-
bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
-
bioRxiv - Plant Biology 2023Quote: ... The membrane was probed with 1:10000 anti-His antibody (Sigma, USA) followed by 1:20000 anti-mouse antibody (Sigma ...
-
bioRxiv - Biophysics 2023Quote: ... His-Clathrin was labeled with Atto594 NHS-ester (ATTO-TEC, Sigma-Aldrich) according to a previously published protocol (41 ...
-
bioRxiv - Plant Biology 2023Quote: ... in-frame of the C-terminal (His)6-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a:ApTPS1 ...
-
bioRxiv - Biophysics 2023Quote: ... then incubated with mouse anti-His tag primary antibody (Millipore Sigma SAB1305538) at 1:1,000 dilution ...
-
bioRxiv - Plant Biology 2024Quote: ... and confirmed by Western blotting using His-tag antibody (Sigma; SAB 1305538). The His-tagged GUN1-PS protein was produced essentially as described (19) ...
-
bioRxiv - Immunology 2024Quote: ... and 5% Fetal bovine serum (FBS; Sigma Heat Inactivated, US/HI origin). PBMCs were cultured overnight in chemotaxis media containing RPMI and 1% ultrapure BSA prior to use in assays.
-
bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich) at 37°C ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... or human liver microsomes (HLMs) (male human pooled, Sigma-Aldrich), respectively ...
-
bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
-
bioRxiv - Immunology 2024Quote: ... light chain and the heavy chains of the included HLA types were expressed in bacterial Bl21 (DE3) pLysS strain (Novagen, cat#69451) and purified as inclusion bodies ...
-
bioRxiv - Cancer Biology 2020Quote: ... Human haemoglobin (Sigma) was used for creation of standard curve.
-
bioRxiv - Microbiology 2021Quote: ... human insulin (Sigma), 10’000 units/ml of penicillin and 10’000 µg/ml streptomycin (Sigma) ...
-
bioRxiv - Microbiology 2021Quote: ... human HDL (Millipore), human LDLs (Millipore ...
-
bioRxiv - Microbiology 2021Quote: ... human LDLs (Millipore) or an in vitro reconstituted NS1-HDL mix were analyzed by size exclusion chromatography on a Superdex 200 10/300 column (GE healthcare) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Human hemoglobin (Sigma) was dissolved in PBS to 10 mg/mL or 1 mg/mL ...
-
bioRxiv - Microbiology 2023Quote: ... Human DPP4 (Sigma) was used as a control in PBS at 50 ng/μL (0.585 μM) ...
-
bioRxiv - Cell Biology 2020Quote: ... The fractions containing the complex were pooled and concentrated in a 100kDa molecular weight cutoff concentrator (Millipore). The protein was then used immediately to prepare grids for cryo-EM.
-
bioRxiv - Biophysics 2019Quote: ... The ARL6:GTP complex was then loaded onto 3 ml of anti-Flag M2 affinity resin (Sigma). The resin was washed with 30 ml of buffer + GTP to remove any excess ...
-
bioRxiv - Biophysics 2021Quote: ... 18 μL of Pt catalyst (Platinum(0)-2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane complex solution) (Sigma-Aldrich, Cat#479543) and one drop of cross-linking modulator 2,4,6,8-Tetramethyl-2,4,6,8 -tetravinylcyclotetrasiloxane (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2021Quote: ... the Peroxidase-Anti-Peroxidase (PAP) Soluble Complex antibody produced in rabbit (Sigma-Aldrich, St. Louis, United States) was used at a dilution of 1:20’000 ...
-
bioRxiv - Cell Biology 2022Quote: The following antibodies were used for immunoblot analyses: peroxidase-anti-peroxidase (PAP) soluble complex (Sigma-Aldrich; P1291); rabbit polyclonal anti-Myc (GeneScript ...
-
bioRxiv - Biochemistry 2022Quote: The TSEN•CLP1 (0.74 mg/mL) complex was crosslinked with 75 μM bis(sulfosuccinimidyl)suberate (BS3; Sigma). The wt-TSEN complex (1.6 mg/mL ...
-
bioRxiv - Neuroscience 2022Quote: ... Complex lipid removal was achieved incubating the samples in a 2:1 mixture of dichloromethane (Sigma-Aldrich) and methanol overnight ...
-
Impact of DNA sequences in the DNA duplex opening by the Rad4/XPC nucleotide excision repair complexbioRxiv - Biochemistry 2020Quote: ... Purified crosslinked complex eluted at 400–480 mM NaCl and was further concentrated by ultrafiltration (Amicon, Millipore) to ∼3.5 mg/ml (30 μM).
-
bioRxiv - Biophysics 2023Quote: ... The purified NaV1.6EM complex was eluted with buffer D (buffer C plus 5 mM desthiobiotin (Sigma, USA)) and was subsequently concentrated to 1 mL using a 100 kDa cut-off Amicon ultra centrifugal filter (Merck Millipore ...
-
bioRxiv - Cell Biology 2023Quote: ... 18 μL of Pt catalyst (Platinum(0)-2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane complex solution) (Sigma-Aldrich, Cat#479543), 5 µL of cross-linking modulator 2,4,6,8-Tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane (Sigma-Aldrich ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... before the complex was immobilized by on column binding to an Anti-FLAG antibody packed resin (Sigma). After washing the resin with 9 column volumes wash buffer (20 mM HEPES ...
-
bioRxiv - Cell Biology 2023Quote: ... 18 μL of Pt catalyst (Platinum(0)-2,4,6,8-tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane complex solution) (Sigma-Aldrich, Cat# 479543), and one drop of cross-linking modulator 2,4,6,8-Tetramethyl-2,4,6,8 -tetravinylcyclotetrasiloxane (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2023Quote: ... Arp2/3 iso-complex was incubated with 100 µM CK-666 or CK-869 (Sigma, Bio-Techne), or equivalent amount of DMSO at room temperature for 1 hour before being added to the experimental system.
-
bioRxiv - Genetics 2024Quote: ... were lysed with 175 μl of cold cytoRNA solution (50 mM Tris HCl pH 8.0; 140 mM NaCl; 1.5 mM MgCl2; 0,5% NP-40; 2mM Vanadyl Ribonucleoside Complex; Sigma) and incubated 5′ in ice ...
-
bioRxiv - Molecular Biology 2024Quote: ... The complex was purified using its incubation with 1 ml of anti-FLAG M2 affinity gel (Sigma) 6h at 4CC ...
-
bioRxiv - Molecular Biology 2020Quote: ... a single fluorescent cell was sorted per well of a fibronectin-coated (10 μg/mL Human Plasma Fibronectin Purified Protein (Sigma Aldrich) in 1x PBS ...