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Citations for Millipore Sigma :
101 - 150 of 9513 citations for HBSS Solution since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: Cells were washed with HBSS/Ca/Mg and switched to a staining solution containing a proprietary fluorescent autophagosome marker (Sigma). The cells were then incubated at 37°C in 5% CO2 for 20 minutes ...
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bioRxiv - Genomics 2021Quote: Frozen eggs or juveniles were resuspended in 500 µL Hank’s balanced salt solution (HBSS, Sigma-Aldrich, Cat. #H4641, Lot RNBG1861) and crushed with a glass Dounce homogenizer for 7 min ...
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bioRxiv - Neuroscience 2021Quote: ... the cerebral hippocampi and the cortexes were isolated stereoscopically in Hank’s Balanced Salt Solution (HBSS) and digested by papain (20 U/mL, Sigma-Aldrich) for 30 min at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were pelleted by centrifugation and washed twice with Hanks’ Balanced Salt solution (HBSS, Cat. No H6648, Sigma-Aldrich), resuspended in HBSS and the single cells encapsulated for library preparation and sequencing using the Chromium single cell platform (10X Genomics Inc. ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor fragments were then transferred to a solution of type V collagenase (Sigma C9263, 1 mg/mL in 1X HBSS) supplemented with soy trypsin inhibitor (Gibco ...
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bioRxiv - Cancer Biology 2022Quote: ... and tumor fragments were transferred to a solution of type V collagenase (Sigma C9263, 1 mg/mL in HBSS 1X) and incubated at 37 C for 45 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... in 37°C for 15 minutes followed by trituration to make single cell suspension in HBSS/DNase/soybean trypsin inhibitor solution (Millipore-Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... forelimb and hindlimb buds of 12.5 dpc Prx1-Cre negative or Cre positive homozygous Axin1 flox embryos were collected in Hanks Balanced Salt Solution (HBSS, Sigma) and digested with 0.1% trysin ...
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bioRxiv - Immunology 2022Quote: ... the colon was washed with cold PBS and minced into 0.5cm long pieces before incubation in chelating buffer Hanks’ Balanced Salt Solution (HBSS) (Sigma Aldrich) with 5% foetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... mice were euthanized by CO2 overdose and the femurs and tibias were collected in ice-cold Hank’s balanced salt solution (HBSS, Sigma-Aldrich). The muscles were removed with a scalpel and by rubbing the bones with a tissue ...
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bioRxiv - Neuroscience 2023Quote: ... the embryos excised from the sac and the brains separated from their heads on ice-cold Hank’s Balanced Salt Solution (HBSS) (Sigma, Ireland). The mesencephalon was removed from each brain ...
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bioRxiv - Immunology 2023Quote: ... 15 mL of pre-warmed digestion solution (1X HBSS with Ca+ and Mg, 5% FBS, 50 U/ml Dispase II [Millipore-Sigma ...
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bioRxiv - Immunology 2023Quote: ... 15 mL of pre-warmed digestion solution (1X HBSS with Ca+ and Mg, 5% FBS, 50 U/ml Dispase II [Millipore-Sigma, cat # 4942078001] ...
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bioRxiv - Microbiology 2023Quote: ... 14 hours post-infection the cells were washed 2x with Hanks□ Balanced Salt solution (HBSS, Sigma-Aldrich Cat. Nr. H8264) and fixed with 4 % PFA in PBS (Ampliqon Cat ...
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bioRxiv - Immunology 2023Quote: ... and then digested in 7 ml Hank’s balanced salt solution (HBSS) with 0.5% bovine serum albumin (Sigma-Aldrich, Cat# A2153), 2% FCS ...
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bioRxiv - Pathology 2023Quote: Mouse epidydimal adipose tissue was harvested and digested in Hanks’ Balanced Salt Solution (HBSS, no calcium and magnesium) containing fetal bovine serum (FBS,3%) and collagenase D (2.5 mg/ml, Sigma-Aldrich) for 30 minutes at 37°C under agitation ...
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bioRxiv - Neuroscience 2023Quote: Ipsilateral and contralateral L3–L5 DRG were surgically removed and immersed in cold Hanks’ Balanced Salt Solution (HBSS, Sigma-Aldrich). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... Ca2+-free experiments were performed by omitting Ca2+ from the HBSS solution and by supplementing the medium with EGTA (0.3 mM; Sigma-Aldrich). Perturbation of Ca2+i levels was performed by loading iMG with the cell-permeable Ca2+ chelators BAPTA-AM and EGTA-AM ...
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bioRxiv - Cell Biology 2023Quote: ... hippocampi were dissected from P2 mouse pups in cold Hanks’ balanced salt solution (HBSS) supplemented with 0.08% D-glucose (Sigma-Aldrich), 0.17% Hepes ...
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bioRxiv - Physiology 2020Quote: ... and after two washes with ice-cold HBSS containing CaCl2 (HBSS, Sigma, St. Louis, MI, USA), the pellet was resuspended in 7 mL and filtered (Cell Strainer 70 um Nylon ...
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bioRxiv - Cell Biology 2023Quote: ... the HBSS was aspirated and replaced with HBSS with calcium and magnesium plus glutamate (50μM: Sigma) which was also added to cell-free wells to determine the starting glutamate concentration ...
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bioRxiv - Microbiology 2022Quote: ... At 48 hours post-transfection the cells were washed 3x with Hanks⍰ Balanced Salt solution (HBSS, Sigma-Aldrich Cat. Nr. H8264) and fixed with 4 % PFA in PBS (Ampliqon Cat ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Colonic innervating DRGs were isolated from the T9-L2 region of the spinal cord of wild type and transgenic animals and immediately placed in cold Hanks′ balanced salt solution (HBSS; Sigma-Aldrich). Isolated DRGs were initially digested with HBSS containing L-cysteine (0.3 mg.ml-1 ...
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bioRxiv - Immunology 2021Quote: ... Bone marrow isolations were done by collecting left and right tibia and flushing bones with 5ml Hanks Balanced Salt solution (HBSS) (Sigma-Aldrich) using a syringe with a 26G needle ...
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bioRxiv - Cell Biology 2023Quote: ... flasks were pre-coated with 2 μg per cm2 using a solution of 50 μg/ml collagen from calf skin in HBSS (Sigma-Aldrich) and the cells were grown in DMEM supplemented with 10% FCS ...
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bioRxiv - Neuroscience 2023Quote: ... Cortical pieces were then washed briefly in HBSS/10 mM HEPES twice and transferred to MEM solution consisting of MEM (Sigma M4655) with 10 mM HEPES (Gibco 15630-080) ...
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bioRxiv - Molecular Biology 2021Quote: ... Propargylamine (Sigma, 1 mM in HBSS) was then added ...
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bioRxiv - Physiology 2020Quote: ... 10× HBSS without sodium bicarbonate (Sigma) was diluted to 1× concentration in sterile tissue culture-grade water ...
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bioRxiv - Neuroscience 2020Quote: ... in HEPES-buffered saline (HBSS, Sigma) for 90 min at 37 °C and then triturated down to single-cell level using glass Pasteur pipettes of decreasing size ...
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bioRxiv - Bioengineering 2022Quote: ... 50 μL HBSS (#H6648; Sigma-Aldrich) was added to the observation windows to prevent gel dehydration and to increase optical clarity ...
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bioRxiv - Genomics 2023Quote: ... in HBSS buffer (H6648, Sigma-Aldrich)) ...
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bioRxiv - Bioengineering 2023Quote: ... HBSS + 0.1% TritonX-100 (Sigma-Aldrich) + 1.5 μg/mL Wheat Germ Agglutinin Alexa Fluor 555 Conjugate plasma membrane stain (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... HBSS + 0.1% TritonX-100 (Sigma-Aldrich) + 1.5 μg/mL Wheat Germ Agglutinin Alexa Fluor 555 Conjugate plasma membrane stain (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... diluted in HBSS buffer (Sigma; H6648) supplemented with 20 mM HEPES (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... containing 15 mL enzyme digestion solution (10 mM HEPES, 0.2 U/mL Liberase TM [Roche 5401127001], and 30 μg/mL DNase I [Sigma D5025] in HBSS). Tissue dissociation was carried out using the intestine C-tube protocol on a gentleMACS Octo Dissociator (Miltenyi 130-095-937 ...
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bioRxiv - Genomics 2020Quote: ... and tissue was enzymatically digested in a solution (1X HBSS, 20mM HEPES, 30mM sodium bicarbonate, 1% BSA fraction V) containing collagenase type IV (200 U/mL; Sigma C-5138); hyaluronidase Type IS (1mg/mL ...
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bioRxiv - Developmental Biology 2022Quote: ... were diced into fragments and digested with digestion solution (0.25% dispase, 0.25% collagenase type II in HBSS (Sigma-Aldrich, Cat. No: H6648)) (5ml in total ...
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bioRxiv - Neuroscience 2021Quote: ... no magnesium (HBSS) (Sigma-Aldrich, MO, USA) for 5 min at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Growth media was replaced with HBSS (Sigma) with 1%FBS and 10mM HEPES (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... after perfusion of the pancreas through the common bile duct with 5-10mL of calcium-free Hanks-buffered saline solution (HBSS) supplemented with 0.4mg/mL Type V collagenase (C9263, Sigma, St. Louis, MO, USA). After overnight culture ...
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bioRxiv - Cell Biology 2022Quote: ... were washed three times using Hank’s balanced salt solution (HBSS) supplemented with 1% of penicillin/streptomycin mix (P/S, Sigma Aldrich/Merck, Poznan, Poland). The endometrium was dissociated from myometrium ...
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bioRxiv - Neuroscience 2021Quote: ... further dissociated with enzymatic solution (0,01% Papain, Sigma, 10108014001; 0,1% dispase II, Sigma, D4693; 0,005% DNAseI, Sigma, 10104159001; 12,4 mM MgSO4 in HBSS) for 20 min at 37 °C and filtered through 70 µm nylon mesh (Miltenyi ...
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bioRxiv - Microbiology 2022Quote: ... and cultured in six-well plates were washed twice using Hanks’ Balanced Salt Solution (HBSS, BioWhittaker, Verviers, Belgium) with 0.15% 1-mg/ml TPCK trypsin (Sigma-Aldrich, St. Louis, USA), then inoculated using the diluted BALF samples and incubated for one hour in 37 ℃ at 5% CO2 incubator ...
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bioRxiv - Microbiology 2023Quote: ... Intestinal tissues were then finely chopped and digested in complete HBSS-2 (HBSS, 25 mM HEPES, 1 mM sodium pyruvate) containing 0.05 mg/mL Collagenase VIII (Sigma) and 50 mg/mL DNase I (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were washed twice with 100μL/well HBSS prior to addition of 80μL/well assay buffer (HBSS (Sigma H8264) + 0.1% BSA) ...
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bioRxiv - Molecular Biology 2024Quote: ... Fixed cells were washed twice with HBSS containing 2 % FBS before resuspension in HBSS containing 0.1 mg/mL propidium iodide (Sigma), 2 mg/mL RNAseA (Thermo) ...
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bioRxiv - Neuroscience 2021Quote: ... harvested whole brains were cut and minced into small pieces and incubated in 10 mL Hanks’ Balanced Salt Solution (HBSS) with calcium and magnesium supplemented with 1 mg/mL collagenase B (Sigma-Aldrich, St. Louis, MO) at 37°C for 30 min with intermittent shaking ...
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bioRxiv - Physiology 2019Quote: ... Animals were sedated using thiopentone and perfused through the portal vein with HBSS solution followed by DMEM low glucose containing Collagenase A (340 μg/ml) (Sigma Aldrich Cat. no # C5138). The tissue was minced in this solution and passed through a 70 µm strainer to obtain a single cell suspension ...
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bioRxiv - Microbiology 2020Quote: ... Influenza virus infection media was composed of a single IAV strain diluted to the appropriate MOI in HBSS for A/WSN/33 and in HBSS including 1 µg/mL TPCK-trypsin (Sigma) for A/California/04/09 and A/Hong Kong/8/68 ...
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bioRxiv - Genomics 2021Quote: ... suspensions of PHH and both NPC fractions were washed and filtered at least twice in ice-cold 1X HBSS with-out Ca2+ and Mg2+ (HBSS w/o, Sigma) to remove tissue and cellular debris and to get individual cells in media that is compatible with the downstream experimental steps ...