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Citations for Millipore Sigma :
251 - 300 of 3645 citations for Ferritin light chain FTL Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... cells were transferred to medium containing light lysine (L5501, Sigma-Aldrich), via three rapid and gentle washes in 30 °C pre-heated medium without lysine at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... plates were covered in light halocarbon oil (Halocarbon Oil 27, Sigma). Embryos with a distinctive faint halo in the periphery ...
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bioRxiv - Synthetic Biology 2024Quote: ... an appropriate amount of light mineral oil (Sigma-Aldrich, Cat#: M5904) was added to the lipid film and vortexed thoroughly to ensure the lipid is dissolved ...
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bioRxiv - Immunology 2020Quote: ... or human (1:20000; anti-human IgG; Sigma) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... mouse monoclonal antibody against slow myosin heavy chain I (MYH1, UniProtKB Q5SX40. Sigma-Aldrich, M8421), rabbit monoclonal antibody against α-smooth muscle actin (α-SMA ...
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bioRxiv - Biochemistry 2019Quote: ... Lyophilized nisin powder (2.5% nisin content) and insulin chain B were obtained from Sigma-Aldrich. The leader peptide of NisA was synthesized and obtained from JPT peptide technologies ...
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iDePP: a genetically encoded system for the inducible depletion of PI(4,5)P2 in Arabidopsis thalianabioRxiv - Plant Biology 2020Quote: ... 2 ml of 50 % HIS-Select® Cobalt affinity resin (Sigma) was washed with milli-Q water and then with equilibration buffer (20 mM imidazole ...
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bioRxiv - Biochemistry 2019Quote: ... The cell lysate was combined with His-Select Co2+ resin (Sigma) for 1 hour at 4 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... complete His-Tag Purification Resin (NiNTA) were purchased from Sigma Aldrich. Protein inhibitor cocktail (PIC ...
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bioRxiv - Biochemistry 2021Quote: ... anti-TtSmtB antibodies (GeneCust™) and His-tag antibodies (Sigma-Aldrich). His-tag removal from recombinant TtSmtB was performed as previously described (22).
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 800 mM) ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 300 mM) ...
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bioRxiv - Microbiology 2021Quote: ... and a combination of α-6×His antibody (Sigma Aldrich, H1029) and HisProbe-HRP (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag was removed using an Enterokinase Cleavage Capture kit (Novagen), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
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bioRxiv - Plant Biology 2022Quote: PIF3 recombinant proteins were prepared using PIF3-his DNA (pET28C, Novagen) provided by Ferenc Nagy and induced and purified using commercial kit (Amersham) ...
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bioRxiv - Microbiology 2021Quote: ... His-tag removal was carried out by overnight HRV-3C (Sigma) digestion at 4°C and the cleaved protein was then purified on Ni-NTA before being subjected to SEC on Superose 6 10/300 column (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: His-ACBD5 was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG overnight at 18°C ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tag was cleaved by digestion with thrombin (Sigma-Aldrich) in the presence of 2 mM CaCl2 at 37°C for 2 hours ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Microbiology 2021Quote: ... Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt, Germany). Rhodamine B isothiocyanate-Dextran (RITC-Dextran ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins were purified using Ni2+-containing His-Bind columns (Novagen). Purified proteins were desalted and concentrated by ultracentrifugation (Amicon ...
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bioRxiv - Cell Biology 2020Quote: ... followed by immunoblotting with HRP-conjugated anti-His antibody (Sigma-Aldrich) by using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067) by overnight incubation at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... –Ade (20 ug/mL L-His HCl monohydrate (A-9126, Sigma) added to –Trp ...
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bioRxiv - Immunology 2021Quote: ... either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich) 0.1M HEPES ...
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bioRxiv - Microbiology 2021Quote: ... probed using 1:500 monoclonal anti-His HRP-conjugated antibodies (Sigma) and imaged using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... the ORF of eggpl was inserted into vector piztv5-His (Novagen) in our laborary to overexpress eggpl ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
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bioRxiv - Biochemistry 2023Quote: ... and supernatant was bound to Ni-NTA His-Bind Resin (Novagen) for 1hr at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was loaded onto Ni-resin (HIS-Select, Sigma-Aldrich) and washed with buffer 1 (50 mM Hepes-KOH pH7.6 ...
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bioRxiv - Biochemistry 2022Quote: ... at 4 °C for 40 minutes and filtered before purification of TlyA by sequential Ni2+-affinity (Cytiva HisTrap™ FF crude 1mL or manual His-column using Millipore Ni-NTA His-Bind® Resin) and gel filtration (Cytiva HiLoad™ 16/600 Superdex™ 75 ...
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bioRxiv - Biochemistry 2021Quote: ... The “light” versions of the SILAC amino acids were obtained from Sigma. For Inducible expression of FLAG-PTP1B ...
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bioRxiv - Cell Biology 2020Quote: ... Light media additionally included 30 µg/mL lysine (Sigma-Aldrich L5626-100G), and 5 µg/mL arginine (Sigma-Aldrich A5131-100G) ...
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bioRxiv - Cell Biology 2020Quote: ... All blots were developed using Carestream Kodak BioMax Light Film (Sigma-Aldrich).
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bioRxiv - Neuroscience 2019Quote: ... strong odor test trial (acetophenone diluted 1:1000 light mineral oil; Sigma). The inter-trial interval was three minutes.
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bioRxiv - Cell Biology 2019Quote: ... and either light Arg and Lys or heavy Arg (13C6 15N4, Sigma) and Lys (13C6 15N2 ...
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bioRxiv - Molecular Biology 2020Quote: ... N-hydroxysuccinimide (NHS) and light mineral oil were purchased from Sigma-Aldrich. GoTaq G2 Hot-start polymerase ...
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bioRxiv - Cancer Biology 2022Quote: ... Media was supplemented with labelled amino acids: light condition [Lys0 (Sigma, L8662), Arg0 (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Polymerase chain reaction (PCR) samples were prepared using KAPA2G Fast PCR Kit (Sigma St. Louis, MO) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... and 1 mM polyP-45 (average chain length 45 Pi units; Sigma-Aldrich, Oakville, ON, Canada) in Ham’s F12 medium for 30 minutes or medium alone (control) ...
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bioRxiv - Cell Biology 2021Quote: ... with or without BSA-complexed long-chain fatty acids (LCFAs: FA-free BSA (Sigma-Al-drich), Palmitic Acid (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... Incoming side chain protected amino acid was activated with HBTU (Sigma-Aldrich, St Louis, MO, USA) in dimethylformamide (DMF ...
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bioRxiv - Biochemistry 2022Quote: ... as a His-tag fusion and Ars2206-531 into pRSFduet vector (Novagen) as a Strep-tag fusion ...
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bioRxiv - Molecular Biology 2019Quote: ... Monoclonal anti-His and anti-Flag antibodies were obtained from Sigma Aldrich, St ...
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bioRxiv - Microbiology 2019Quote: ... The supernatant was mixed with Ni2+-NTA His•Bind resin (EMD Millipore) equilibrated with Buffer G and allowed to go overnight at 4 °C ...