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Citations for Millipore Sigma :
501 - 550 of 714 citations for Feline Infectious Peritonitis Virus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The virus particles in the supernatant were concentrated to 100μl using Amicon® Ultra-15 Centrifugal Filters (Sigma-Aldrich Chemicals Private Limited ...
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bioRxiv - Immunology 2021Quote: ... The supernatant containing pseudo-virus was centrifuged at 3000g and filtered through a 0.45μm sterilized membrane (Millipore, Burlington, MA). The titer of virus generated by engineered Zhou-COVID-19-Spike plasmid is ten-fold higher than non-engineered Spike protein sequence (data not shown) ...
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bioRxiv - Microbiology 2021Quote: ... cell monolayers were washed twice with PBS before adding virus inoculum in DMEM supplemented with 1μg/ml of trypsin TPCK (Sigma) and no FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... HUVECs were transduced with optimal volume of lentiviral virus at 50% confluence in MV2 medium and 8μg/ml Polybrene (Sigma). After 24 h ...
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bioRxiv - Biochemistry 2022Quote: The virus was precipitated from the cell supernatant by adding 8 % (w/v) polyethylene glycol (PEG) 8000 (Sigma-Aldrich), incubating at +4 °C with gentle mixing for 3 h ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
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bioRxiv - Immunology 2023Quote: ... Next the pseudotyped virus-containing culture medium was diluted 50% in culture medium supplemented with 8μgml−1 polybrene (Sigma) and immediately applied onto MutuDC1 cells ...
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bioRxiv - Microbiology 2023Quote: ... Virus supernatant was removed 6 hours post transduction and cells were maintained for 48 hours before puromycin (Sigma-Aldrich) selection ...
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bioRxiv - Microbiology 2023Quote: ... Confluent MDCK cells were incubated with each serial dilution for 1h at 37°C then input virus was removed by aspiration and cells overlaid with SFM containing 0.14% BSA (Sigma), 0.8% Avicel© (FMC BioPolymer ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transduced with the respective virus particles in an equal ratio using 5 µg/mL polybrene (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... concentrated virus particles were freshly thawed and diluted in phosphate-buffered saline (PBS, Merck, 806552, Sigma-Aldrich, Darmstadt, Germany) based on the p24 antigen concentration in the supernatant ...
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bioRxiv - Biochemistry 2023Quote: ... Virus-containing supernatants were harvested after 48 h transfection and filtered by syringe through a 0.45 μM filter (Millipore) to eliminate cell contaminates ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were transduced with the virus for 48 h in the presence of 4 µg/ml polybrene (Sigma H9268).
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bioRxiv - Microbiology 2023Quote: ... neutralized whole-virus samples were incubated in D2O buffer (99% deuterium oxide, Sigma 151882, supplemented with 120 mM NaCl). 40 μL D2O buffer was added to every 10 μL of neutralized virus (final D2O concentration 78% ...
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bioRxiv - Cancer Biology 2023Quote: ... subconfluent cultures were incubated with virus-containing supernatants in the presence of 4µg/ml polybrene (Sigma-Aldrich , TR-1003) for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... virus-containing supernatants were used to transduce A2EN cells in the presence of 8 µg/ml polybrene (Sigma-Aldrich). Cells were co-transduced with lentiviral vectors encoding the two distinct sgRNAs that target the same gene ...
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bioRxiv - Microbiology 2023Quote: ... The medium for the culture of influenza A virus in MDCK cells (virus medium) was prepared by supplementing Minimal Essential Medium with 4.3% bovine serum albumin (BSA; Sigma) and Normocin.
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Microbiology 2024Quote: ... The virus particles were further purified by layering them over a discontinuous 5% to 50% Nycodenz gradient (Sigma-Aldrich) in TNE buffer ...
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bioRxiv - Genetics 2023Quote: ... RPE-1 cells were transduced with lentivirus at an MOI of 0.3-0.5 (usually between 50-100 µL of virus/well) and 10 µg/mL Polybrene (Sigma-Aldrich). After 48 hours ...
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bioRxiv - Physiology 2024Quote: ... C2C12 mouse myoblasts were seeded 24h prior to infection and then transduced with virus-containing supernatant supplemented with 8μg/mL polybrene (Millipore) as shown previously40 ...
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bioRxiv - Microbiology 2024Quote: All transductions were performed via spin inoculation at 1050 x g for 2 hr at 25°C with equal virus amounts determined by Gag p24 mass in medium containing 4µg/mL polybrene (Sigma). MDMs were inoculated with 10µg p24 mass equivalents of NL4-3 ΔGPE-GFP virus or 20µg p24 mass equivalents of 3xFLAG-89.6-Vpr ...
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bioRxiv - Cell Biology 2020Quote: ... hydrated sections were subjected to antigen retrieval by pronase (1 mg/ml in PBS containing 5 mM calcium chloride; Sigma-Aldrich) incubation for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: Soluble egg antigen (SEA) of LE was extracted by mechanical lysis (Funakoshi motorized pestle; Diagnocine, Hackensack, NJ) in RIPA buffer (Millipore Sigma) supplemented with protease inhibitors (Protease Inhibitor Cocktail I ...
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bioRxiv - Immunology 2022Quote: ... Sections were washed 3 times in PBS followed by antigen retrieval for 20 minutes with steam using 1X Citrate buffer (Millipore, 21545), pH=6.0 ...
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bioRxiv - Immunology 2022Quote: ... Slides were subsequently boiled with antigen retrieval buffer (Tris-EDTA, pH9, made in house or citrate buffer, pH6, Sigma C999-1000mL) and blocked for one hour with PBS containing 1% bovine serum albumin (BSA) ...
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bioRxiv - Cancer Biology 2019Quote: ... the TMA slide was subjected to Cell Conditioning 1 (CC1, Ventana) antigen retrieval and stained with a prediluted anti-MCT antibody (Mouse, Clone G3, Millipore #MAB1222) using an automated immunostainer ...
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bioRxiv - Immunology 2022Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 10 min and antigen retrieved using proteinase K (Sigma-Aldrich, P6556) in Tris-EDTA pH8 buffer for 4 min at room temp ...
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bioRxiv - Developmental Biology 2021Quote: ... boiled in citric acid (pH 2.0) for antigen retrieval and stained with an α-MHC antibody (Sigma My-32, 1:1000) or α-GFP antibody (Abcam ab13970 ...
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bioRxiv - Microbiology 2020Quote: Infected cells grown on coverslips were fixed with 4% paraformaldehyde and permeabilized/blocked with a Triton-X100 (0.2%)/BSA (3%) solution and then incubated with mouse anti-Flavivirus Group Antigen 4G2 (Millipore, Burlington, MA), mouse anti-alphavirus capsid (kindly provided by Dr ...
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bioRxiv - Physiology 2020Quote: ... 5 μm tissue sections were subject to antigen retrieval (0.01 M citric acid and 0.1% Triton X) (Sigma, St. Louis, MO), blocked with the avidin/biotin blocking kit (Vector Laboratories) ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections were subjected to a heat-induced antigen retrieval step prior to exposure to primary OSM antibody (1:50, HPA029814, Sigma-Aldrich). Immunodetection was performed using the Roche Ventana BenchMark ULTRA IHC/ISH staining system ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 8 and the α-NOCT antibody was antigen affinity purified with NOCT (64-431) immobilized on Immobilon-P PVDF membrane (Millipore). Guinea pig α-NOCT was eluted from the membrane with 0.1 M glycine and 20% glycerol ...
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bioRxiv - Genetics 2022Quote: Longitudinal paraffin-embedded sections were processed as described (Gomez et al., 2022) following 1-hour antigen retrieval with 2mg/ml Hyaluronidase (Sigma-Aldrich) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (clone PAb416, Sigma Aldrich - Saint-Quentin-Fallavier France) diluted 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... rat anti-PDGFRβ (eBiosciences 14-1402-81), rabbit anti-AQP3 (Alomone Labs, AQP-003, after antigen retrieval) rabbit anti-Runx1 (Sigma, HPA004176), rabbit anti-IRF9 (Cell Signalling ...
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bioRxiv - Genetics 2023Quote: ... Antigen retrieval was performed by pressure cooker incubation of slides in 1L of pre-warmed 1X Citrate Buffer (Sigma Aldrich #C9999) + 0.1% Tween in dH2O for a total of 15 mins (human ...
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bioRxiv - Microbiology 2023Quote: ... at 15 days and 36 days with 1:1 (v/v) mixture of antigen with incomplete Freund’s adjuvant (Sigma-Aldrich, USA). After the first immunization ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were immediately subjected to heat-mediated antigen retrieval at 95°C for 10 minutes in 1X pH6.0 sodium citrate buffer (Sigma-Aldrich, #21545) using Micromed T/T Mega Multifunctional Microwave Rapid Histoprocessor (Milestone) ...
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bioRxiv - Immunology 2023Quote: Eosinophilic airway inflammation was induced by two intraperitoneal injections of 5μg of cockroach antigen (CA, Greer Laboratory, Lenoir, NC) mixed in 100μl of alum adjuvant (aluminum hydroxide, Sigma, St. Louis, MO) at day 0 and 7 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by antigen retrieval using Tris EDTA buffer pH 9 (Genemed, 10-0046) or 1x sodium citrate at pH 6 (Sigma, C9999) according to manufacturer’s recommendation for specific antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... coverslips were first incubated with Tris-glycine solution 5min at room temperature followed by blocking of non-specific antigens with a cold-water fish blocking buffer (Sigma-Aldrich) for 30min ...
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bioRxiv - Immunology 2021Quote: ... a sandwich ELISA was used in which 1% Trixon X-100-treated virus lysates were added to plates pre-coated with ConA (50 µg/ml, Sigma) and reacted with anti-Env mAbs 66 ...
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bioRxiv - Molecular Biology 2021Quote: ... HEC-1-B cells and HEK293T cells at the confluency of 70%∼90% were infected with the virus in the presence of 8 μg/ml polybrene (Sigma). Puromycin (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... were transduced when reaching around 50-70% confluence with 1x concentrated virus diluted in ADF+++ and supplemented with hEGF and 5 µg/µl polybrene (Sigma). The cells were incubated for 12-18 h with the viral suspension ...
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bioRxiv - Cell Biology 2019Quote: ... The virus-containing medium was then used to infect RAWs in the presence of 8 μg/mL polybrene (Sigma-Aldrich) for 24 h ...
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bioRxiv - Microbiology 2021Quote: ... The parental HEK293T cells were transduced with this virus and the HEK293T-ACE2 cell line was selected and maintained with medium containing puromycin (Sigma). ACE2 expression was confirmed by immunofluorescence staining using mouse monoclonal antibody against c-Myc antibody 9E10 (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... Virus (genome equivalent of a MOI of 1) was added in the presence of 8 μg/ml polybrene (Sigma Aldrich) and the cells were incubated on ice for 1⍰ h to allow virus binding ...
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bioRxiv - Neuroscience 2021Quote: ... N2a cells’ media was exchanged with virus-containing supernatant at 48- and 72-hours post-transfection with 8µg/ml polybrene (Sigma #H9268). N2a cells were expanded and FACS sorted for mCherry+ cells.