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Citations for Millipore Sigma :
51 - 100 of 5772 citations for Fab ZAP rabbit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... All Fabs were buffer exchanged to TBS using 10 kDa MWCO concentrators (Millipore).
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bioRxiv - Developmental Biology 2021Quote: ... Samples were incubated with Anti-digoxigenin-AP Fab Fragment (Sigma-Aldrich, 11093274910, RRID: AB_514497) in blocking solution ≥14 hrs at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Conjugated Fabs were then concentrated using an Ultrafree 0.5 filter (10k-cut off; Millipore) to 1 mg/ml ...
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bioRxiv - Genetics 2022Quote: ... anti-Digoxigenin-AP Fab fragments was purchased from Sigma (Roche # 11 093 274 910) and used at 1:10,000 (75 mU/ml).
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bioRxiv - Plant Biology 2020Quote: ... 46 by replacing the antibody with primary anti-Digoxigenin Fab fragment (Sigma-Aldrich cat# 11214667001) and secondary donkey anti-sheep IgG (H+L ...
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bioRxiv - Microbiology 2021Quote: ... Fabs for neutralization and inhibition assays were generated by digesting IgG with papain (Sigma, P4762) and purifying as described previously67 ...
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bioRxiv - Immunology 2020Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate in 1:5000 PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Fab were concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (NMWL 10 kDa; Millipore) to about 1 mg ml−1 ...
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bioRxiv - Cancer Biology 2022Quote: Bi-Fabs were incubated with an excess of TCEP (20 eq, Sigma Aldrich, Gillingham, UK) to fully reduce the interchain disulfides ...
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bioRxiv - Molecular Biology 2023Quote: ... Fab were concentrated in an Amicon Ultra-0.5 Centrifugal Filter Unit (NMWL 10 kDa; Millipore) to >1 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... Fraction from the peak corresponding to the Fab were concentrated using Amicon Ultra-15 (Millipore) and further purified by size-exclusion chromatography using Superdex 200 Increase 10/300 GL (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... Antigen-reactive mAbs were detected with goat anti-human IgG (Fab)-horseradish peroxidase (Sigma-Aldrich) conjugate diluted in PBS at a ratio of 1:5000 ...
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bioRxiv - Biochemistry 2022Quote: F61 and D2 Fab fragments were prepared by digesting F61 and D2 IgG with papain (Sigma), respectively ...
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bioRxiv - Genomics 2022Quote: ... The hybridized probe was immunodetected with antidigoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich, 11093274910) and visualized with a CDP star (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Thiolated Fab fragments were purified using 30 kDa molecular weight Amicon ultra filtration columns (Millipore, #UFC503096) and the concentration was estimated by A280 absorbance using Nanodrop 2000 (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... Fractions containing the SOSIP/Fab complexes were pooled and concentrated using 10 kDa Amicon spin concentrators (Millipore). Samples were diluted to 0.02 mg/mL in TBS (0.05 M Tris pH 7.4 ...
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bioRxiv - Immunology 2021Quote: ... concentrated using Amicon Ultra-4 50-kDa (IgG) or 30 kDa (Fab) centrifugal filter units (Millipore Sigma) and stored at 4°C until use ...
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bioRxiv - Biochemistry 2022Quote: ... The aE11-Fab sample was concentrated by centrifugal concentrator with a MWCO of 3 kDa (Merck Millipore) and further purified by size exclusion chromatography into 20 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... DIG epitopes were detected with HRP anti-DIG fab fragments at 1:2500 (Sigma-Aldrich; Reference 11207733910) and revealed using Cy3-tyramide at 1:100 ...
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bioRxiv - Immunology 2023Quote: ... The flow through containing the Fab was concentrated through 10 kDa Amicon centrifugal filter units (EMD Millipore) and buffer exchange was performed with a 7 kDa molecular weight cut-off size exclusion resin (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescein epitopes were detected with HRP anti-fluorescein fab fragments at 1:5000 (Sigma-Aldrich; Reference 11426346910) and revealed using Cy2-tyramide at 1:250 ...
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bioRxiv - Genetics 2023Quote: ... The hybridized probe was immunodetected with anti-digoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich; 11093274910) and visualized with a Bmpurple (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... The hybridized probe was immunodetected with anti-digoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich; 11093274910) and visualized with a CDP star (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... Fabs were concentrated up to ~1 mg/mL using an Amicon Ultra 0.5 filter (10k cut-off, Millipore) and conjugated with CF640 or Cy3 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were transferred into a 24-well plate filled with a FAB solution (0.7% Formaldehyde (FA, 36.5-38%, Sigma) with 0.15% or 1% acrylamide (AA ...
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bioRxiv - Immunology 2022Quote: ... Unliganded BG24S60A Fab was concentrated to 10-15 mg/mL by centrifugation with a 30-kDa concentrator (Millipore).
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bioRxiv - Cell Biology 2019Quote: ... The resulting Fabs were concentrated up to ∼1 mg/ml in PBS using a Microcon filter unit (Millipore) and labeled with NHS-ester of Alexa Fluor 488 (ThermoFisher) ...
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bioRxiv - Immunology 2020Quote: ... Fractions containing the SOSIP/Fab complexes were pooled and concentrated using 10 kDa Amicon® spin concentrators (Millipore). Samples were diluted to 0.03 mg/mL in TBS (0.05 M Tris pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... Fractions containing the SOSIP/Fab complexes were pooled and concentrated using 10 kDa Amicon® spin concentrators (Millipore). Samples were diluted to ∼0.03 mg/mL in TBS (0.05 M Tris pH 7.4 ...
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bioRxiv - Molecular Biology 2021Quote: ... Excess Fab was removed by ultrafiltration in TBS pH 7.4 with a 100 kDa cutoff Centricon filter (Amicon Ultra, Millipore), and the sample was concentrated to ∼5 mg/mL for cryo grid freezing ...
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bioRxiv - Microbiology 2022Quote: ... of 3.5 mg/mL mixtures of purified STFK or STFK1628x proteins in complex with excess Fab fragments of nAbs were incubated in 0.01% (v/v) Digitonin (Sigma) and then loaded onto glow-discharged (60 s at 20 mA ...
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bioRxiv - Microbiology 2022Quote: SEC-purified LCMV pfGP-TD and LCMV pfGP-TD/M28 Fab complex in TBS was concentrated to 0.8 and 1.2mg/mL using a 30K Amicon centrifugal filter (Sigma Aldrich). For LCMV pfGP-TDapo ...
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bioRxiv - Genetics 2023Quote: ... The hybridized probe was immunodetected with antidigoxigenin Fab fragments conjugated to alkaline phosphatase (Cat # 11093274910, Sigma-Aldrich, MO, USA) and visualized with a CDP star (Cat #11685627001 ...
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bioRxiv - Neuroscience 2020Quote: ... the blots were incubated with secondary antibody horseradish peroxidase (HRP)-conjugated goat anti-human Fab IgG (H+L) (Sigma, A0293) diluted 1:10000 in blocking buffer for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... After 2 h at RT plates were washed three times and incubated with anti-human Fab specific-HRP conjugated secondary antibody (Sigma) for 1 h at RT ...
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bioRxiv - Biophysics 2019Quote: ... and the Fabs were concentrated to a concentration of 0.28 mg/mL using a centrifugal filter unit (Sigma, # UFC501024, Switzerland).
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bioRxiv - Microbiology 2020Quote: ... 15 µl of secondary antibody (anti human Fab-HRP: Jackson ImmunoResearch, cat# 109-035-097; anti human IgG-HRP: Sigma, cat# A6029-1ML ...
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bioRxiv - Neuroscience 2022Quote: ... Dig epitopes were detected with alkaline phosphatase-coupled anti-Dig fab fragments antibody at 1:2500 (Millipore Sigma, cat. #11093274910), and the signal revealed with Nitro Blue Tetrazolium chloride/5-Bromo-4-Chloro-3-Indolyl Phosphate ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The 1% BSA in PBST was then used to dilute sheep anti-digoxygenin Fab-fragments conjugated to alkaline phosphatase (1:1000, Sigma-Aldrich) in which the tissues were incubated overnight at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... excess Fab was removed by filtration using 100 kDa molecular weight cut-off size exclusion Amicon ultra filtration columns (Millipore, # UFC510096). Subsequently ...
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bioRxiv - Neuroscience 2021Quote: ... Thioflavine S (T1892), Anti-Digoxigenin-AP (Fab fragments, 11093274910), and Phorbol 12- myristate 13-acetate (PMA, p8139) were ordered from Sigma-Aldrich.
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bioRxiv - Zoology 2023Quote: ... tissues were hybridized with digoxigenin labeled antisense DNA and subsequently exposed to anti-digoxigenin Fab’ fragments conjugated to alkaline phosphatase (Sigma-Aldrich) and peptide primary antisera ...
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bioRxiv - Bioengineering 2022Quote: ... Anti-fluorescein-AP Fab fragment antibody (cat N° 11426338910) for the detection of fluorescein-labeled lectin was obtained from Sigma-Aldrich. FITC-Concanavalin A lectin was purchased from Vector Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was submerged in secondary antibody solution (TBS-T + 5% BSA + 1:3000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody (Sigma-Aldrich) and incubated for two hours at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Buffer exchanged the elution to PBS using 30-kDa cut-off Amicon ultrafiltration units.For Fab preparation: incubate 500 μg of concentrated polyclonal IgG samples with papain (Sigma Aldrich P3125) in digestion buffer (100 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... Parasites were washed thrice for 15 min in PBSTx and incubated for 24 hrs in 1:2000 Fab specific FITC conjugated goat anti human IgG (F5512, Sigma-Aldrich UK) diluted in blocking buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... They were then incubated for 2-hour incubation with a blocking solution at room temperature and subsequently incubated with anti-digoxigenin-Rhodamine (Anti-digoxigenin-Rhodamine, Fab fragments, Sigma, Cat# 11207750910) (0.5μg/mL ...
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bioRxiv - Immunology 2023Quote: ... ELISA signal in each elution sample was checked using 1:5000 diluted goat anti-human IgG (Fab specific) HRP-conjugated secondary antibodies (Sigma-Aldrich, A0293-1ML). Elution fractions showing an ELISA signal were pooled and concentrated under vacuum to a volume of ∼1 μL ...
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bioRxiv - Cancer Biology 2019Quote: ... The Fab and Fc were buffer exchanged with PBS and concentrated using Amicon Ultra centrifugal filter (7 kDa mwco, Millipore Sigma, St. Louis, MO).
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bioRxiv - Developmental Biology 2020Quote: ... Samples were then washed six times with TNT for at least 20 min each and then subjected to second-round probe detection by either anti-DIG-POD Fab fragments (Sigma-Aldrich; Cat. No. 11207733910) or Anti-Fluorescein-POD Fab fragments (Sigma-Aldrich ...