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Citations for Millipore Sigma :
401 - 450 of 9906 citations for FSTL1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% heat-inactivated Fetal Bovine Serum (FBS-hi; Sigma, Cat. no. F7524), 1% antibiotic-antimycotic (AA ...
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bioRxiv - Biophysics 2021Quote: ... The plasmids were incorporated into pcDNA3.1(+) vectors and were transiently transfected into HEK 293T cells using PEI (Milipore Sigma) as previously described 66 ...
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bioRxiv - Molecular Biology 2021Quote: ... TIFs (Telomerase immortalised fibroblasts29) and human embryonic kidney (HEK)293Ts cells were cultured in Dulbecco’s Modified Eagles Medium (DMEM) (D5796, Sigma). All cell culture medium was supplemented with 10% v/v foetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Human HEK-293T cells were grown in Dulbecco’s Modified Eagle’s high glucose medium with HEPES (Sigma-Aldrich®, D6171) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... HEK-293T cells were transfected with gene of interest for 24 hrs and treated with CHX (100μg/ml; Sigma). Cell lysates were prepared at indicated time points and subjected to 10% SDS-PAGE followed by western analysis as described above.
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bioRxiv - Genetics 2021Quote: Human embryonic kidney (Hek) 293T cells were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS, Sigma), 1% penicillin-streptomycin (P/S ...
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bioRxiv - Biochemistry 2020Quote: ... adherent human embryonic kidney (HEK) 293T cells were transfected with the expression plasmids with branched polyehtyleneimine (PEI, Sigma-Aldrich). In detail ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK 293T cells were transfected with a 1:3 ratio of DNA:polyethylenimine (PEI, 25 kDa linear, Sigma-Aldrich). Transfection of siRNA was performed using X-tremeGENE siRNA Transfection Reagent (Roche ...
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bioRxiv - Biophysics 2022Quote: ... The plasmids were incorporated into pcDNA3.1(+) vectors and were transiently transfected into HEK 293T cells using PEI (Milipore Sigma) as previously described 32 ...
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bioRxiv - Microbiology 2022Quote: ... HEK 293T-hACE2-TMPRSS2 cells (called herein HEK-ACE2-TMPRSS2) were induced for TPMRSS2 expression by addition of doxycycline (0.5 μg/mL, Sigma) and were maintained in DMEMc with blasticidin (10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: The Env expression plasmids were used to transfect HEK-293T cells with X-tremeGENE HP DNA Transfection Reagent (Sigma) in combination with either a Tat expression plasmid pTat for Env expression and fusion assays ...
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bioRxiv - Molecular Biology 2021Quote: The lysates of HEK-293T cells transfected with circPTPN12 plasmid were incubated with Protein-A/G agarose beads (Millipore) and antibody against Ago2 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Human embryonic kidney (HEK) 293T cells and chicken DF-1 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Sigma) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... human embryonic kidney (HEK) 293T cells and chicken DF-1 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Sigma) supplemented with 10% FBS and 100 U/ml Penicillin-Streptomycin (complete DMEM) ...
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bioRxiv - Biochemistry 2022Quote: Expression of recombinant extracellular proteins was performed by transient transfection in HEK−293F cells using polyethylenimine (PEI, Sigma Aldrich). 300 mL of culture at a density of 1.0 × 106cells/mL was transfected with 300 μg DNA using 450 μg PEI ...
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bioRxiv - Biophysics 2022Quote: Human embryonic kidney cell line (HEK-293T) were seeded on 25 mm No.1.5 H cover glass (Marienfeld) coated with poly-L-lysine (Sigma). 0.15 million cells were maintained in 2ml Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Genetics 2023Quote: Human embryonic kidney (HEK) 293T cells were cultured in high glucose DMEM containing 10% fetal bovine serum (FBS, Sigma), 1% penicillin-streptomycin (P/S ...
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bioRxiv - Cell Biology 2023Quote: ... and human embryonic kidney (HEK) 293T cells authenticated by RIKEN were cultured in Dulbecco’s modified Eagle’s medium (DMEM; D6546; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2021Quote: ... ACER3-wt or ACER3-E33G expression was induced in FreeStyle 293-F(TetR) cells with 1 µg/mL doxycycline (Sigma). Induced cells were counted and resuspended at 0.5×106 cells/mL in medium containing vehicle (0.0125% DMSO ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were produced by transfection of 293-6E cells (National Research Council Canada) using branched polyethylenimine (PEI) 25kDa (Sigma-Aldrich). Cells were maintained at 37°C and 6% CO2 FreeStyle 293 Expression Medium (Thermo Fisher ...
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bioRxiv - Bioengineering 2019Quote: Ni-NTA columns were prepared by packing 1 mL Ni-NTA His•Bind resins (Sigma) into a 6-ml ISOLUTE® Single Fritted Reservoir column with 10 μm polyethylene frit (Biotage ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tag was cleaved by overnight incubation with thrombin (from bovine plasma, Sigma Aldrich) at 4 °C during dialysis ...
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bioRxiv - Bioengineering 2020Quote: ... His tagged FLIPsuc sensors were purified by affinity chromatography using Ni-NTA bead material (Novagen). Binding to the resin was performed in batch at 4 °C for 4 h ...
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bioRxiv - Biochemistry 2020Quote: ... His-PBP1B containing fractions were pooled and treated with 2 U/mL of thrombin (Novagen) for 20 h at 4 °C during dialysis against dialysis buffer I (25 mM Tris-HCl ...
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bioRxiv - Biophysics 2020Quote: ... or 8 μM His-tagged NSP2 labelled with 8 μM Atto488-nitrilotriacetic acid (NTA, Sigma), and buffer (0.5 × phosphate saline buffer ...
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bioRxiv - Immunology 2022Quote: ... and 100 µL of alkaline phosphatase-conjugated anti-HIS tag antibody (Sigma-Aldrich, cat: A5588), diluted 1:2,000 in TBST ...
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bioRxiv - Microbiology 2021Quote: ... PfArlH or biotinylated PfArlI were subsequently mixed with HIS-Select® Nickel Affinity resin (SIGMA) while lysate of cells overexpressing Strep-SaArlI was mixed with Streptactin column material (IBA GmbH ...
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bioRxiv - Biochemistry 2021Quote: ... DTT was added to a final concentration of 0.5 mM and His-SUMO protease (Sigma) was added to a concentration 100-fold lower than the total protein concentration ...
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bioRxiv - Physiology 2020Quote: ... the N-terminal-His-tag was labeled with the Ni-NTA Atto550 fluorophore (Sigma-Aldrich) following the protocol provided by the manufacturer ...
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bioRxiv - Bioengineering 2020Quote: ... The eluted His-tagged RBD was then incubated with biotin-tagged HRV C3 protease (Sigma) and passed through a streptavidin-agarose column to deplete the protease and the 8XHis-SBP peptide ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were subjected to purification using either HIS-Select HF Nickel Affinity Gel (Sigma-Aldrich) or Glutatione Sepharose 4B (GE healthcare) ...
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bioRxiv - Plant Biology 2020Quote: ... and purified with a commercial kit according to manufacturer’s instructions (His-bind buffer kit, Millipore).
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bioRxiv - Immunology 2022Quote: ... coli and protein was isolated and purified on His-binding nickel resin (Novagen, Madison, Wisconsin). Nitrophenyl-haptenated ovalbumin (NP-OVA ...
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bioRxiv - Biochemistry 2023Quote: Strep-Full-length REC114 and His-MEI41-127 were co-expressed from pRSFDuet-1 (Novagen) and pProEXHTb (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... For super-shift assays monoclonal 0.2 μl of Anti-His antibody (H1029, Sigma-Aldrich, USA) was used ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then added to 3 mL of HIS Select Nickel affinity gel (Sigma) equilibrated in wash buffer (10 mM HEPES ...
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bioRxiv - Plant Biology 2023Quote: ... with a disruption pressure of 2.35kbar and then purified using His-bind buffer kit (Millipore) following manufacturer’s recommendations.
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bioRxiv - Biochemistry 2022Quote: ... and the supernatant was purified by affinity chromatography using Ni-NTA His-bind resin (Novagen). The column packed with the resin was equilibrated with binding buffer ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was then added to 3 mL of HIS Select Nickel affinity gel (Sigma) equilibrated in wash buffer (10 mM NaPO4 ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted His-tagged σNS was concentrated using a 10-kDa centrifugal filter unit (Millipore), dialyzed into 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Plant Biology 2023Quote: ... pH 7.4 and then bound to poly-prep chromatography columns containing His-bind resin (Novagen). The yeast lysate mixture was loaded in the columns ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% v/v heat-inactivated fetal bovine serum (HI-FBS) (Sigma Aldrich, F7524), Penicillin-Streptomycin 100U/ml (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with 1 µg/mL His-EqtII-EGFP and DAPI (D9542, SIGMA) at room temperature for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... and His-AC elution buffer were supplemented with 0.1 v/v% Tween-20 (Sigma Aldrich), 0.1 v/v% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... containing HI-2 at final concentration of 100 µM (or vehicle control DMSO, Sigma-Aldrich) was added to the cells ...
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bioRxiv - Molecular Biology 2024Quote: ... His-OGT-Flag was generated by subcloning the OGT cDNA into pET30a+ vector (Novagen®). siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic® ...
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bioRxiv - Microbiology 2024Quote: ... The cell lysate was then incubated with HIS-select HF Nickel Affinity Gel (Millipore-Sigma) overnight ...
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bioRxiv - Microbiology 2024Quote: ... The cell lysate was then incubated with HIS-select HF Nickel Affinity Gel (Millipore-Sigma) overnight ...
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bioRxiv - Biophysics 2020Quote: ... The plasmids were transiently transfected to the TMEM16F-KO HEK 293T cells by using X-tremeGENE9 transfection reagent (Millipore-Sigma). Cells grown on poly-L-lysine (PLL ...
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bioRxiv - Neuroscience 2021Quote: ... 1mL of HEK 293F cells from each batch was mixed together in a 12-well Corning Costar Not Treated Plate (Millipore) and incubated at 37°C with shaking on Orbi-Shaker (Benchmark ...