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Citations for Millipore Sigma :
651 - 700 of 6672 citations for FLI1 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... The recombinant protein was then concentrated using centrifugal filter units (Amicon Ultra-4, Millipore) and dialyzed using Slide-A-Lyzer dialysis cassette into storage buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human recombinant MAO-A (hMAO-A) and MAO-B (hMAO-B) enzymes (Sigma-Aldrich) were diluted in 50 mM phosphate buffer (final protein amount ...
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bioRxiv - Evolutionary Biology 2020Quote: ... ephyrae were placed in ASW supplemented with 500 nM human recombinant insulin (Sigma I0908). Insulin was refreshed weekly ...
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bioRxiv - Cell Biology 2020Quote: ... HMWHA or BCM− was pre-incubated with 300 IU/mL recombinant HYAL2 (Sigma-Aldrich) for 30 min prior to addition to co-cultures.
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat ...
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bioRxiv - Cell Biology 2021Quote: Human recombinant TNFα and LPS (from Escherichia coli O111:B4) were purchased from Sigma. VCAM-1 (sc-13160) ...
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bioRxiv - Molecular Biology 2020Quote: ... Digestion was performed overnight at 37 °C using recombinant dimethylated SOLu-trypsin (Sigma-Aldrich) with a trypsin:protein ratio 1:20 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 500 U/mL ESGRO recombinant mouse Leukemia Inhibitory Factor (LIF) protein (Sigma, ESG1107) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... Purified recombinant S proteins were concentrated using Amicon filtration devices (EMD Millipore, Billerica, MA), buffer-exchanged into PBS and analyzed by SDS-PAGE and Western blotting ...
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bioRxiv - Cell Biology 2021Quote: ... The recombinant protein was purified by affinity chromatography using anti-FLAG affinity resin (Sigma). The purified protein was precipitated by 20% trichloroacetic acid ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant RNase R (30 pmols) or Bovine Serum Albumin (BSA, Sigma-Aldrich, MO, USA) were alone or combined with Csm5 WT or Csm5Δ46 (90 ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Molecular Biology 2022Quote: We concentrated recombinant EGFP fusion proteins using Amicon Ultra centrifugal filters (10K MWCO, Millipore) to an appropriate protein concentration in 250 mM NaCl salt buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 200 µM AMP (Serva) and active recombinant AMPK (16 ng) (Sigma-Aldrich; 14-840) in a total volume of 30 µl ...
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bioRxiv - Cancer Biology 2022Quote: ... recombinant SHH (C24II) (50 ng/ml, R and D) and Purmorphamine (1.5 μM, Sigma). Plates were then incubated at 37°C at 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... these were supplemented with 2 ng/ml recombinant human FGF basic protein (Sigma-Aldrich), 15 mM sodium bicarbonate ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich, USA), and 20 ng/ mL human bFGF (Life Technologies)] with 10 μM Rho kinase inhibitor Y-27632 (Wako ...
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bioRxiv - Developmental Biology 2023Quote: ... This basal medium was completed with 5ng/ml recombinant mouse LIF (Sigma ref LIF2050), 3µM CHIR99021 (Sigma ref SML1046-5MG ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant proteins were concentrated using an Amicon Ultra (10K; Millipore, Billerica, MA, USA).
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bioRxiv - Microbiology 2024Quote: Recombinant ϕLf-UK PSB15 protein was expressed in B834 (DE3) pLysS cells (EMD Millipore) by transforming pR-PSB15 ...
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bioRxiv - Immunology 2024Quote: ... aliquots of 106 splenocytes were treated with recombinant mouse IL-2 (Sigma, cat # 11271164001) at 5-20 units/mL and cultured in 48 well plate for 30 minutes at 37C ...
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bioRxiv - Cell Biology 2023Quote: ... Normal Rabbit IgG (12-370, Sigma-aldrich, Rabbit polyclonal), Normal Mouse IgG (12-371 ...
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bioRxiv - Immunology 2021Quote: ... MAb was reacted with antigen coated on the plates and then treated with serially diluted C1q from human serum (Sigma). C1q binding was detected with horseradish peroxidase-conjugated anti-C1q antibody ...
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bioRxiv - Biochemistry 2021Quote: ... mAbs were concentrated using Amicon Ultra-4 Centrifugal Filter Units with a 50 kDa cut-off (Millipore Sigma Cat # UFC805024) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... the supernatants were subjected to immunoprecipitation with anti-FLAG mAb magnetic beads (Catalog No. M8823, Sigma-Aldrich, St. Louis, MO) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... Germany) were precoated with murine anti-HIS mAb at a dilution of 1:2,500 (v/v; Sigma-Aldrich, Munich, Germany) at 4°C overnight ...
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bioRxiv - Bioengineering 2020Quote: ... or mouse monoclonal β-actin antibody (1:1000 dilution; catalog no. catalog no. MAB 1501; Merck Millipore, Burlington, MA, USA) at room temperature for 1 hour ...
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bioRxiv - Genetics 2021Quote: ... glial fibrillary acidic protein (GFAP; polyclonal 1:1000, ZO334, DAKO-Agilent, Santa Clara, CA, USA; monoclonal 1:1000, MAB 3402, Millipore, Burlington ...
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bioRxiv - Immunology 2020Quote: ... 400 μl containing 300 PFU/ml of SARS-CoV-2 virus were then added to the mAb solution supplemented with 0.25% guinea pig complement sera (Sigma, Israel) and the mixture incubated at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Purified mAbs were concentrated and buffer changed into PBS using Amicon® Ultra-4 50KDa Centrifugal Filter Units (Millipore Sigma). Purified mAbs in PBS were then filtered using sterile 0.2-μm pore size filter devices (Millipore) ...
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bioRxiv - Biochemistry 2022Quote: ... Each mAb was diluted to a final concentration of 0.32 μg/μL with guanidine hydrochloride (Sigma, 5 M final concentration) and reduced into Lc and Hc with DTT (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... and 200 μl of (in 10 μg/mg mAb concentration) Papain (Papain from papaya Latex, 89% pure lyophilized powder from Sigma). The mixture was then kept in the incubator shaker at 37 degrees Celsius for 8-10 hours ...
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bioRxiv - Biochemistry 2023Quote: Mouse monoclonal anti-FLAG M2 (#F3165) and anti-CD95 mAb CH-11 (#05-201) was from sigma-Aldrich (Sigma Aldrich Chimie ...
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bioRxiv - Microbiology 2023Quote: The subtype of the mAb was IgG1 as determined using a subtype identification kit (Sigma-Aldrich, St. Louis, MO, USA). Non-immunoglobins in the ascites were removed by precipitation with caprylic acid ...
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bioRxiv - Cell Biology 2024Quote: Antibodies that were used in this study to detect viral and cellular proteins were the monoclonal antibody (MAb) recognizing the FLAG-tag epitope anti-Flag (clone M2, Sigma), the MAb against HCMV pp28 ...
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bioRxiv - Cell Biology 2020Quote: ... The in vitro interaction experiments using recombinant Ana1 fragments and commercial GST-PBD (Sigma, SRP0360) were carried out as described previously (Alvarez-Rodrigo et al. ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich) induction ...
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bioRxiv - Genomics 2020Quote: ... 3.33 ml 75 mg/ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
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bioRxiv - Developmental Biology 2019Quote: ... 1000U/ml ESGRO® recombinant mice leukemia inhibitory factor (LIF, Millipore, ESG1107, stock 107U/ml) and 2i (1μM PD325 ...
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bioRxiv - Cell Biology 2019Quote: ... growth media was replaced with media containing 50ng/ml BMP-12 (recombinant human, Sigma Aldrich) and 10ng/ml IGF-1 (recombinant human ...
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bioRxiv - Plant Biology 2019Quote: ... The purified recombinant proteins were concentrated using Amicon centrifugal filters following the manufacturer recommendations (Millipore). Protein yield was estimated by measuring absorbance at 595 nm using a spectrophotometer (UV-2600 ...
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bioRxiv - Neuroscience 2021Quote: ... the astrocytes were stimulated with TNF recombinant (10 ng /ml) (Sigma-Aldrich Corporation, Missouri, EUA) or MCM (300 µl ...
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bioRxiv - Neuroscience 2021Quote: ... cells were stimulated with human TNF recombinant (10 ng/ml) (Sigma-Aldrich Corporation, Missouri, EUA) for 24 hours.
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bioRxiv - Biochemistry 2020Quote: ... The supernatant containing recombinant proteins were incubated with anti-FLAG M2 affinity gel (Sigma, A2220) for 2 hours on a rotator at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant INO80 complex was eluted with buffer B containing 1.6 mg Flag Peptide (Sigma-Aldrich). Anion exchange chromatography (MonoQ 5/50 GL ...
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bioRxiv - Developmental Biology 2021Quote: ... 10 μg/ml BSA fraction V and 10 μg/ml human recombinant insulin (both Sigma). Fluorescence was measured by flow cytometry 24 h after the addition of RA.
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bioRxiv - Developmental Biology 2021Quote: ... 10 μg/ml BSA fraction V and 10 μg/ml human recombinant insulin (both Sigma)) ...
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bioRxiv - Microbiology 2019Quote: Recombinant L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin was purchased from Sigma. The protease inhibitor dec-RVKR-CMK was obtained from Tocris ...
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bioRxiv - Cell Biology 2021Quote: ... coli DH10B cells and cells harboring recombinant plasmids selected on Luria Bertani (LB; Sigma-Aldrich) agar plates with 50 μg/ml kanamycin ...
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bioRxiv - Cell Biology 2022Quote: ... subcutaneously with 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, Germany) followed by a boost after 4 weeks ...