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Citations for Millipore Sigma :
301 - 350 of 10000+ citations for FAS Associated Death Domain Protein FADD Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were detected with primary antibodies to FLAG M2 (Sigma, A8592), HA 3F10 (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... using myelin basic protein primary antibody (MERCK-Millipore, Massachussets, United States).
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STARCH SYNTHASE 4 is required for normal starch granule initiation in amyloplasts of wheat endospermbioRxiv - Plant Biology 2021Quote: ... Antibodies were enriched from antiserum using protein A-agarose (Sigma-Aldrich). Affinity purification of TaBGC1-specific antibodies from the antiserum was performed with a HiTrap NHS-Activated HP column (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... The tagged proteins were immunoprecipitated using FLAG M2 antibody beads (Sigma). Co-precipitating RNAs were reverse transcribed using random hexamers and Superscript III (Life Technologies) ...
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bioRxiv - Systems Biology 2020Quote: ... Co-immunoprecipitated proteins were detected using anti-Flag antibody (M2 – Sigma), eGFP fusion protein levels monitored using anti-GFP antibody (ab290 – abcam ...
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bioRxiv - Genetics 2020Quote: ... Protein extraction and Western blot using anti-puromycin antibody (Merck Millipore) was performed as described before.
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bioRxiv - Plant Biology 2022Quote: ... proteins were detected with alkaline phosphatase-conjugated antibody (Sigma-Aldrich A3687). Primary antibodies used were ...
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bioRxiv - Cell Biology 2021Quote: ... and mouse GAPDH monoclonal antibody for protein loading controls (Sigma, G8795). Anti-mouse IRDye680RD and anti-rabbit IRDye800CW (Licor ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies were recovered by binding to protein A/G agarose (Millipore), and the eluted DNA fragments were used directly for qPCR or subjected to high-throughput sequencing (ChIP-Seq ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against the following proteins were used: FLAG (Sigma Aldrich F1804), Streptavidin-Alexa Fluor 488 (Life S32354) ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected by a monoclonal anti-FLAG antibody (Sigma, USA) or a polyclonal anti-GFP antibody (GenScript ...
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bioRxiv - Genetics 2023Quote: ... PREPL proteins were immunoprecipitated using anti-FLAG M2 antibody (Sigma Aldrich) and separated by SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitinated proteins were detected using an anti-FLAG antibody (F7425, Sigma), HA-TAP-Gb1 and endogenous Gb1-4 were detected with anti-HA (16B112 ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies and biotinylated Hyaluronic Acid Binding Protein (HABP, #385911 Millipore) were incubated for 72 hours at 4°C or 24 hours at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... The extracellular domain (residues 1–270) of BF2*1901 were cloned into a pET21a vector (Novagen) and transformed into Escherichia coli strain BL21 (DE3) ...
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bioRxiv - Molecular Biology 2023Quote: ... coli N-terminal domain (NTD; residues 1-130) of ProQ were cloned into pET15b vector (Novagen), as described (Stein et al ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were detected by western blotting using the following antibodies: Anti-GFP antibody (1:3000 dilution; Millipore), anti-aldolase antibody (1:3000 ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used: PDT-tagged proteins - anti-HA antibody (Sigma, cat# 11666606001, dilution 1:1000), mf-Lon - anti-Flag (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were detected using commercially available antibodies: anti-Flag mouse Antibody M2 (1:1000, Sigma-Aldrich M8823) and rabbit anti 4R-tau Antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were lysed and processed for cell death detection ELISA assay by following the manufacturer’s instruction (#11774425001, Millipore Sigma). At the end of the assay ...
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bioRxiv - Genetics 2022Quote: ... Cell death analysis was performed using the ApopTag® Fluorescein in situ Apoptosis Detection Kit (Sigma-Aldrich, Cat. No. S7110) following manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: Secreted lactate dehydrogenase (LDH) activity was measured as a correlate of cell death in culture supernatants using Cytotoxicity Detection KitPLUS (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: Cell death was assessed using the TdT-mediated dUTP nick end-labeling (TUNEL) method (ApopTagPeroxidase In Situ Apoptosis detection kit, Millipore) as per manufacturers’ instructions ...
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bioRxiv - Biochemistry 2023Quote: Apoptotic cell death was detected using the The ApopTag Plus Fluorescein In Situ Apoptosis Detection Kit (Sigma, S7111, supplemental methods).
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bioRxiv - Biophysics 2024Quote: ... The slides were then rinsed with PBS (3×) and then stained with an in situ cell death detection (FITC-TUNEL) staining kit (Millipore), an AlexaFluor-647 anti-collagen antibody (NovusBio ...
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bioRxiv - Cell Biology 2021Quote: Senescence-associated β-galactosidase (SA-β-gal) activity was determined with the Senescence Cells Histochemical Staining Kit (Sigma-Aldrich). Six representative images were captured on a TS100 inverted light microscope (Nikon ...
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bioRxiv - Molecular Biology 2020Quote: ... Singlecell iAT2s were plated on Transwells at a density of 520,000 live cells/cm2 in 100μl of CK+DCI with 10μM Rho-associated kinase inhibitor (“Y”; Sigma Y-27632). 600μl of CK+DCI+Y was added to the basolateral compartment ...
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bioRxiv - Cell Biology 2019Quote: ... and the knee (proximal tibia, distal femur, patella, and associated soft tissues) were dissected out and placed in Ringer’s solution (Sigma) maintained at 25°C with bubbling oxygen with 5% carbon dioxide ...
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bioRxiv - Developmental Biology 2020Quote: ... per manufacturer’s instructions and seeded onto the micropattern at 132,000 – 263,000 cells/cm2 in mTeSR with 10 μM Rho-associated kinase inhibitor (ROCKi Y-27632, Millipore Sigma). After 6h ...
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bioRxiv - Physiology 2022Quote: Senescence-associated β-gal staining was performed with the Cellular Senescence Assay Kit according to the manufacturer’s instructions (Millipore).
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bioRxiv - Genomics 2019Quote: ... RNA-protein complexes were incubated with antibody-bound Protein A Dynabeads (anti-FLAG [Sigma Aldrich F3165] or anti-IgG) at 4C for 2 hours ...
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bioRxiv - Plant Biology 2021Quote: ... and immunoprecipitation of the antibody/protein complexes was performed by adding 50 μl of Protein A-Sepharose (Sigma-Aldrich) made up as a 50% (w/v ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µL of the reaction was removed and quenched by adding 60 µL of 0.75 % formic acid (FA, Sigma) and 0.25 mg/mL porcine pepsin (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were labeled for 30 minutes using 400 µM EU (MRC-5 cells) or for 1 hour with 200 µM EU (HCT116 cells) before fixation with 3.7% formaldehyde (FA, Sigma) in PBS for 15 minutes at room temperature (RT) ...
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bioRxiv - Cell Biology 2022Quote: ... coverslips were carefully submerged into a 24-well plate with 1 ml of 4% Formaldehyde (FA, 36.5–38%, Sigma) and 4% acrylamide (AA ...
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bioRxiv - Physiology 2019Quote: ... 200 μL of incubation media was removed and kept to measure glycerol and FA release using commercial kits (Sigma-F6428 and Wako diagnostic NEFA-HR ...
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bioRxiv - Biochemistry 2022Quote: ... 10 µL of the reaction was removed and quenched by adding 60 µL of 0.75 % formic acid (FA, Sigma) and 0.25 mg/mL porcine pepsin (Sigma ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... 10 µL of the HDX reaction were removed and quenched by diluting 1:6 into 0.75% formic acid (FA, Sigma), and digested for two min with porcine pepsin (0.25 mg/mL ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated at 37°C in 300 μl of Krebs Ringer buffer containing 2% FA-free bovine serum albumin (BSA) and 0.1% glucose in the presence or absence of 10 μM isoproterenol (Sigma) for 1hr.
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bioRxiv - Biochemistry 2023Quote: ... 10 µL of the reaction was removed and quenched by adding 60 µL of 0.75% formic acid (FA, Sigma) and 0.25 mg/mL porcine pepsin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... 10uL of sample was spiked with 10uL of internal standard (IS; L-Trp-d5) and 10uL acidified mobile phase (AMP; 0.2%FA/0.05%TFA/1% acetonitrile (ACN; Millipore-Sigma) in water) ...
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bioRxiv - Immunology 2023Quote: ... The online digestion and trapping were performed for 4 minutes using a flow of 50 µL/min 0.1 % FA (Sigma), pH 2.5 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with 600 μM of OA conjugated with 100 μM of FA-free BSA (A8806; Sigma-Aldrich) for 16 hours ...
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bioRxiv - Immunology 2023Quote: ... The peptides generated by pepsin digestion were subjected to on-line SPE on a PepMap300 C18 trap column (1 mm x 15mm) and washed with 0.1% FA (Sigma) for 60s ...
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bioRxiv - Molecular Biology 2019Quote: ... The DNA-binding domain of human Timeless (DBD; residues 816-954) was cloned into pRSF-Duet1 (Novagen) with an N-terminal His14-SUMO tag and expressed in E ...
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bioRxiv - Biochemistry 2021Quote: ... corresponding to the T1 domain was cloned using NdeI and BamHI sites in the vector pET28a (Novagen) to express a fusion protein with an N-terminal hexahistidine (6xHis ...
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bioRxiv - Cell Biology 2023Quote: The Cno DIL domain sequence (aa 613-1006) was cloned into plasmid pET28 (Millipore Sigma, Burlington, MA) using PCR and primers with engineered NheI and EcoRI restriction sites ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were anti-glial fibrillary acidic protein (GFAP) antibody (dilution; 1:100, Sigma-Aldrich (G6171), St ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used for protein blotting: mouse anti-c-Myc antibody (Sigma-Aldrich, 05-724MG), mouse anti-Flag antibody (Sigma-Aldrich ...