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Citations for Millipore Sigma :
351 - 400 of 5566 citations for FABP2 I FABP Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 800 mM) ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified lysate was applied to Ni-NTA His-Bind Resin (Novagen). Resin was washed with binding buffer and then eluted in batches with buffer containing increasing amounts of imidazole (up to 300 mM) ...
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bioRxiv - Microbiology 2021Quote: ... and a combination of α-6×His antibody (Sigma Aldrich, H1029) and HisProbe-HRP (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag was removed using an Enterokinase Cleavage Capture kit (Novagen), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
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bioRxiv - Plant Biology 2022Quote: PIF3 recombinant proteins were prepared using PIF3-his DNA (pET28C, Novagen) provided by Ferenc Nagy and induced and purified using commercial kit (Amersham) ...
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bioRxiv - Microbiology 2021Quote: ... His-tag removal was carried out by overnight HRV-3C (Sigma) digestion at 4°C and the cleaved protein was then purified on Ni-NTA before being subjected to SEC on Superose 6 10/300 column (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: His-ACBD5 was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG overnight at 18°C ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tag was cleaved by digestion with thrombin (Sigma-Aldrich) in the presence of 2 mM CaCl2 at 37°C for 2 hours ...
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bioRxiv - Plant Biology 2019Quote: ... His-tagged recombinant protein was eluded with 250 mM imidazole (Sigma).
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bioRxiv - Microbiology 2021Quote: ... Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt, Germany). Rhodamine B isothiocyanate-Dextran (RITC-Dextran ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins were purified using Ni2+-containing His-Bind columns (Novagen). Purified proteins were desalted and concentrated by ultracentrifugation (Amicon ...
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bioRxiv - Cell Biology 2020Quote: ... followed by immunoblotting with HRP-conjugated anti-His antibody (Sigma-Aldrich) by using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067) by overnight incubation at 4 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... –Ade (20 ug/mL L-His HCl monohydrate (A-9126, Sigma) added to –Trp ...
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bioRxiv - Immunology 2021Quote: ... either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich) 0.1M HEPES ...
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bioRxiv - Microbiology 2021Quote: ... probed using 1:500 monoclonal anti-His HRP-conjugated antibodies (Sigma) and imaged using a ChemiDoc MP (Bio-Rad) ...
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bioRxiv - Genetics 2022Quote: ... the ORF of eggpl was inserted into vector piztv5-His (Novagen) in our laborary to overexpress eggpl ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... and supernatant was bound to Ni-NTA His-Bind Resin (Novagen) for 1hr at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... or anti-His primary antibodies (dilution 1:3000, #H1029 Sigma Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was loaded onto Ni-resin (HIS-Select, Sigma-Aldrich) and washed with buffer 1 (50 mM Hepes-KOH pH7.6 ...
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bioRxiv - Biochemistry 2022Quote: ... at 4 °C for 40 minutes and filtered before purification of TlyA by sequential Ni2+-affinity (Cytiva HisTrap™ FF crude 1mL or manual His-column using Millipore Ni-NTA His-Bind® Resin) and gel filtration (Cytiva HiLoad™ 16/600 Superdex™ 75 ...
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bioRxiv - Cell Biology 2020Quote: ... and 10µg/ml DNAse I (Millipore). Bacteria were lysed by sonication and clarified by centrifugation at 20,000 xg for 30 min in a JA-20 rotor (Beckman ...
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bioRxiv - Cell Biology 2020Quote: ... and 10µg/ml DNAse I (Millipore). Cleared lysates were loaded onto GST-Trap (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... DNase I (100 μg/mL, Sigma), dispase (0.05 U/mL ...
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bioRxiv - Developmental Biology 2020Quote: ... and then with DNase I (Sigma). 1 µg of total RNA and oligo (dT ...
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bioRxiv - Immunology 2022Quote: ... + 60 units/mL DNase I (Sigma) in DMEM ...
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bioRxiv - Cell Biology 2022Quote: Mitochondrial complex I inhibitor rotenone (Sigma), pan caspase inhibitor Z-VAD (OMe)-FMK (Abcam) ...
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bioRxiv - Molecular Biology 2021Quote: ... treated by DNase I (Sigma D4527) and filtered again through a 40 μm nylon cell strainer (Fisher Scientific #11873402) ...
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bioRxiv - Genomics 2020Quote: ... 100 U/mL DNase I (Sigma) and 39.6 U/mL dispase II (Gibco ...
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bioRxiv - Immunology 2021Quote: ... 10nM Gastrin I (Sigma Aldrich G9020), 50 ng ml−1 EGF (Peprotech AF-100-15) ...
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bioRxiv - Immunology 2021Quote: ... 23 U/mL DNase I (Sigma), 2 mM CaCl2 in PBS ...
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bioRxiv - Biochemistry 2019Quote: ... and 1000U DNase I (Sigma 04716728001). Lysate was centrifuged in a F21-8×50y rotor using a Sorvall RC6+ at 17,000RPM for 25min ...
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bioRxiv - Physiology 2019Quote: ... DNase I (80μg/mL, Sigma Aldrich), and EDTA (20μL/mL ...
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bioRxiv - Neuroscience 2019Quote: ... and Phosphatase Inhibitor Cocktail I (Sigma) and Phosphatase Inhibitor Cocktail III (Sigma ...
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bioRxiv - Physiology 2019Quote: ... and 0.5% collagenase Type I (Sigma) in DMEM/F12 + GlutaMax (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... 10mg/ml DNase I (Sigma 10104159001), 20mg/ml of collagenase (Sigma C2674 ...
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bioRxiv - Immunology 2021Quote: ... 0.125 mg/ml DNase I (Sigma) and 3% FCS for 45 min (lungs ...
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bioRxiv - Developmental Biology 2021Quote: ... with 5% DNAse I (Sigma Aldrich) for 7 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... 100 μg/mL DNase I (Sigma) and 5 mM MgCl2 ...
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bioRxiv - Neuroscience 2019Quote: ... and DNase I (100ug/mL, Sigma) for 15 minutes at 37°C ...
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bioRxiv - Systems Biology 2021Quote: ... 10ug/ml insulin (Sigma #I-1882), 0.5ug/ml hydrocortisone (Sigma #H-0888) ...
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bioRxiv - Cell Biology 2021Quote: ... and DNase I (Sigma-Aldrich DN25). Entire pellets of EGFP-tagged PLCδ1 PH domain and SMT3-SopB33-554 were resuspended in 25 mL as above but contained 300 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... ARPC2 (07-227-I-100UG, Millipore). Secondary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... were treated with collagen I (Sigma, dissolved in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and DNase I (DN25, Sigma, USA), single cells were collected ...
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bioRxiv - Physiology 2022Quote: ... cardiac troponin I (1:100, Millipore), caveolin 3 (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... with dispase-I (Sigma-Aldrich, # D46693) at 4°C for 12-18 hours ...