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Citations for Millipore Sigma :
251 - 300 of 7166 citations for Ephrin type A receptor 8 EphA8 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Developmental Biology 2019Quote: ... 6×His-tagged protein were expressed and purified by Ni-NTA His Bind Resin (Millipore 70666-3). ∼0.2 μg of GST-OsBZR1 ...
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bioRxiv - Microbiology 2023Quote: ... PhoQ-His and Flag-MgrB were detected with the anti-His and anti-Flag primary antibody (Sigma), respectively ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 10% (v/v ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (Sigma Aldrich) supplemented with 10 % fetal calf serum at 37°C/5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (Sigma Aldrich) supplemented with 10% fetal calf serum at 37°C/5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293 suspension cells were transfected with constructs using polyethylenimine (PEI, Sigma). HEK293 suspension cells were cultured at 37°C and 8% CO2 and 120 rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 and HeLa cells were cultured in DMEM media (Sigma, RNBK7590) supplemented with 10% free fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2023Quote: HEK293 (CRL-1573-ATCC) were grown in DMEM (Sigma-Aldrich, USA) supplemented with 10 % FBS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: HEK293 cells were seeded on poly-L-lysine (Sigma, Cat. #P8920) coated coverslips in 12-well plates ...
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bioRxiv - Biochemistry 2023Quote: HEK293 cells were maintained in DMEM F-12 Ham medium (Sigma) supplemented with 10% fetal bovine serum (GIBCO ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 and HeLa cells were transfected with Polyethyleneimine (PEI, Sigma Aldrich) as previously described (Braun et al. ...
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bioRxiv - Neuroscience 2020Quote: ... GABA-A receptor antagonist and Bicuculline (Bic) (Sigma, 285269, USA), were dissolved in CSF tracer before use at a concentration of 0.2 mM ...
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bioRxiv - Neuroscience 2019Quote: ... the GABAB receptor antagonist CGP 55845 (5μM, Sigma cat#SML0594) was added to the bath ...
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bioRxiv - Bioengineering 2021Quote: ... rat monoclonal anti-Nicotinic Acetylcholine Receptor (Sigma-Aldrich, 1:800). The following secondary antibodies (diluted in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... the glutamate receptor antagonist kynurenic acid (4 mM; Sigma– Aldrich) was locally applied in the pretectum by pressure injection through a micropipette fixed to a holder (containing Fast Green to aid visualization of the injection spread) ...
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bioRxiv - Neuroscience 2020Quote: ... an AMPA and kainate receptor blocker (NBQX 10 μM, Sigma), and an inwardly rectifying potassium channel blocker (barium chloride 200 μM ...
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bioRxiv - Neuroscience 2021Quote: ... and D2-like dopamine receptors (Sulpiride, Sulp; 10 μM, Sigma, substances were applied for at least 15 min prior STDP recordings) ...
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bioRxiv - Neuroscience 2020Quote: Mecamylamine hydrochloride (nicotinic receptor antagonist; Sigma (St. Louis, MO, USA)) was applied directly at the surface of the dorsal horn using the agar chamber described above ...
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bioRxiv - Neuroscience 2020Quote: ... and rabbit anti-Opioid Receptor-Mu (Millipore, AB5511, 1:500). The secondary antibodies were donkey anti-rabbit IgG conjugated with Alexa-488 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The TRPV1 receptor agonist capsaicin (3 μM; Sigma-Aldrich, M2028) was applied at the end of experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... using bonafide GABA-A receptor agonists like GABA (Sigma #A2129), Muscimol (Sigma #M1523) ...
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bioRxiv - Neuroscience 2022Quote: ... adrenergic α1B receptor antagonist – Chloroethylclonidine (30 μM, Sigma-Aldrich B003); and adrenergic α1D receptors antagonist – BMY 7378 (10 μM ...
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bioRxiv - Neuroscience 2022Quote: ... a GABAA-receptor antagonist (Sigma−Aldrich, St. Louis, MO, USA) was used ...
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bioRxiv - Neuroscience 2022Quote: ... and strychnine hydrochloride (Strych) (glycine receptor antagonist, 0.3µM, Sigma, #S8753) dissolved in Ringer’s solution ...
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bioRxiv - Molecular Biology 2022Quote: ... antibodies against Epidermal Growth Factor Receptor (EGFR) (Millipore, 06-847) were used at 1:200 dilution at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... 5 µM fibroblast growth factor receptor inhibitor (FGFR, Sigma-Aldrich) and 3 µM GSK inhibitor (CHIR99021 ...
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bioRxiv - Microbiology 2023Quote: ... and the EP2 receptor inhibitor AH6808 at 10µM (Sigma-Aldrich). After washing with PBS-/- ...
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bioRxiv - Microbiology 2023Quote: ... and prostanoid receptor antagonist 2 (AH6809 5mg/kg Sigma-Aldrich) every 2 days or with a 15-PGDH inhibitor (SW033291 at 10mg/kg Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... 1:500 anti-Glutamate receptor AMPA R4 (#G5790, Sigma-Aldrich). Fluorescent secondary antibodies (1:10000 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... All sera were treated with receptor destroying enzyme (RDE; Sigma) for 16-18 hours to reduce non-specific antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... flupenthixol (0.25 mg/kg, nonspecific DA receptor antagonist) (Sigma-Aldrich); SCH-23390 (0.03 ...
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bioRxiv - Neuroscience 2024Quote: ... L-741,626 (10 mg/kg, D2 receptor antagonist) (Sigma-Aldrich). Doses selected were based on previous studies and pilot experiments to control for confounding effects such as motor impairment (Lustberg et al 2020a ...
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bioRxiv - Developmental Biology 2021Quote: ... type VI (Sigma R6625), 1% SDS ...
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bioRxiv - Microbiology 2022Quote: Type IX trypsin (Sigma) for 30 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... collagenase type IV (Sigma) and DNAse I (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Type V neuraminidase (Sigma) was added to a final concentration of 1 U/mL and incubated for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Type III (Sigma-Aldrich), 5 U/mL rabbit muscle L-lactic dehydrogenase ...
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bioRxiv - Molecular Biology 2024Quote: ... Type XI (Sigma-Aldrich), 500 µM phosphoenolpyruvate and 50 µM NADH ...
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bioRxiv - Cancer Biology 2021Quote: ... Kelly human NB cells were transduced with the viral supernatant in the presence of 8 μg/ml polybrene (Sigma-Aldrich) and 24 h later selected using puromycin (1 µg/ml ...
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bioRxiv - Bioengineering 2022Quote: K32 cells (K562 cells expressing human CD32) were transduced with lentivirus expressing WT DLL4 or DeltaMAX by spin-infection using 8 μg/mL polybrene (Millipore). Seventy-two hours after transduction ...
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bioRxiv - Molecular Biology 2021Quote: ... SC-His+3AT is minimal medium of SC-His supplemented with 3-aminotriazole (3-AT, 0.5mM, Sigma-Aldrich).
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bioRxiv - Microbiology 2019Quote: ... Removal of His-tag was verified by western blot with monoclonal α-His –HRP (1:10000) antibody (Sigma).
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bioRxiv - Bioengineering 2019Quote: ... were maintained as monolayer cultures in flasks pre-coated with 60 μg/mL solution of human placental collagen type IV (Sigma Aldrich, MO) and grown in bronchial epithelial growth medium (BEGM ...
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bioRxiv - Plant Biology 2020Quote: ... coli using a Nickel-His column (Sigma). A 5 mL culture was incubated at 37°C for 16 hours ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His tag (HIS.H8, 1:1,000, Millipore). The HRP-conjugated anti-mouse (111–035-144 ...
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bioRxiv - Plant Biology 2020Quote: ... -His with or without 3AT (Sigma-Aldrich). Successful interactions were further screened on –Leu ...
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bioRxiv - Microbiology 2020Quote: ... The anti-his antibody (monoclonal #8722 Sigma) was used at 1/2000 concentration for immunoblot.
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bioRxiv - Biochemistry 2022Quote: ... and anti-His (1:3000) antibody (Sigma). HRP conjugated mouse IgG was used at 1:5000 dilution (Sigma ...