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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Ephrin type A receptor 2 EphA2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 0.5 mM tris (2-carboxyethyl) phosphine (TCEP) and P8849 Protease Inhibitor Cocktail for His-tagged proteins (Sigma). The lysate was cleared at 25.000 × g for 30 min at 4°C and the supernatant was loaded on Protino®Ni-TED 2000 packed columns and purified as described by the manufacturer (Macherey-Nagel ...
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bioRxiv - Biophysics 2021Quote: ... The pelleted crypts were resuspended in Cultrex Matrix (Cultrex PathClear BME Type 2, Sigma-Aldrich) with organoid base media and ROCK inhibitor ...
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bioRxiv - Molecular Biology 2019Quote: ... Panc1 and HEK293 in DMEM (Sigma, Cat No. D1145), SH-SY5Y in 1:1 mixture of DMEM/F12 (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293 cells were transfected with Polyethyleneimine (PEI, Sigma Aldrich) as previously described [46 ...
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bioRxiv - Genomics 2022Quote: ... HEK293 were induced with 3 μg/ml doxycycline (Sigma) and 400 μg/ml of G418 (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293 cells were plated on poly-L-lysine (Sigma) coated coverslips in a 12-well plate and transfected with Kv4.1 or Kv4.2 cDNA ...
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bioRxiv - Developmental Biology 2022Quote: HEK293 cells were cultured in low-glucose DMEM (Sigma) supplemented with 10% FBS (Capricorn Scientific) ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were cultured on poly-D-lysine (Millipore) coated glass coverslips ...
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bioRxiv - Physiology 2019Quote: ... and insulin receptor β-subunit (Millipore 07-724). Plasmids to express flag-tagged PPARγ ...
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bioRxiv - Neuroscience 2020Quote: ... a GABAA receptor blocker (bicuculline 10 μM, Sigma), NMDA receptor blockers (D-AP5 50 μM ...
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bioRxiv - Neuroscience 2020Quote: ... anti-rabbit dopamine D2 receptor (cat# AB5084P, Millipore), anti-rabbit AMPA receptor (GluR1 ...
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bioRxiv - Neuroscience 2022Quote: ... GABAA receptor antagonist picrotoxin (PTX, P1675, Sigma-Aldrich), PKI (6221 ...
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bioRxiv - Neuroscience 2019Quote: ... we injected the GABAA receptor agonist muscimol (Sigma; 8.8 nmol [1µg] or 44 nmol [5 µg] in a 1 µl volume ...
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bioRxiv - Cancer Biology 2021Quote: ... or DMH1 (BMP receptor, Sigma #D8946, 0.5 µM) or EC330 (LIF receptor ...
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bioRxiv - Cancer Biology 2022Quote: ... or the thrombin receptor-activating peptide (TRAP, Sigma) was added to each sample and incubated at room temperature for 15 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... and the GABAB receptor antagonist phaclofen (Sigma, USA) were dissolved in saline and administered at doses of 0.06 nmol/0.2 μL/mouse32 and 0.1nmol /0.2μL/ mouse33 ...
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bioRxiv - Neuroscience 2023Quote: ... M2 muscarinic receptor (rat anti-M2, Millipore, MAB367), Parvalbumin (PV ...
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bioRxiv - Neuroscience 2023Quote: ... GABAA receptor antagonist bicuculline methiodide (#14343; Millipore Sigma) and gabazine (#1262 ...
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bioRxiv - Biophysics 2019Quote: ... His-EmGFP-DBNL and his-mCherry-DBNL were diluted in PBS (D8537, Sigma-Aldrich) to 1 nM each and 30 µl of mixed sample solution was placed on top of a cleaned glass cover-slip for measurement ...
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bioRxiv - Plant Biology 2021Quote: ... Fusion proteins with His tag were purified using Ni-NTA His binding resin (Novagen) and those with GST tag was purified by glutathione sepharose (Novagen).
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... as well as the role of myosin II/RhoA in creating the refractory nature of the retracting losing front to receptor inputs (Fig. 5 and Supplementary Fig. 2) we pre-treated cells with either 50 μM nocodazole (Sigma Aldrich) or 10 μM Υ-27632 (Sigma Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... anti-His (1:1000 Sigma), or anti-StrepII (1:3000 IBA ...
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bioRxiv - Cancer Biology 2019Quote: ... rabbit anti-His (Sigma Aldrich), rabbit anti-Tubulin polyclonal antibody (Cell Signaling Technology).
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bioRxiv - Molecular Biology 2020Quote: ... HIS-tag (Sigma-Aldrich H1029), and Streptactin-HRP (ThermoFisher Scientific #21130) ...
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bioRxiv - Microbiology 2023Quote: The His-tag system (Novagen) was used for Sif purification ...
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bioRxiv - Bioengineering 2021Quote: ... The activated fluidic channels were coated with 0.5 mg/mL collagen type IV human placenta (Sigma Aldrich, C5533-5MG) in the cell culture incubator overnight at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... Chip channels were then coated with a mixture of ECM in Dulbecco’s phosphate-buffered saline (DPBS): 200μg/mL human placenta collagen type IV (Sigma-Aldrich) and 30 μg/mL fibronectin (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼30% CD90+ stromal cells were dissociated at 15 days using human cardiac digestion buffer: 0.2% collagenase type I (Sigma) in 20% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... The pETDuet-1-His-BAP1-ULD+ASXL2-AB protein complex was expressed in Rosetta 2 (DE3) pLysS (Millipore). The bacteria bearing the desired plasmids were propagated with aeration at 37°C in 1L of 2YT to an A600 absorbance of approximately 0.6 ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), equilibrated in lysis buffer and incubated for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
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bioRxiv - Neuroscience 2022Quote: ... 6-cyano-7-nitroquinoxaline-2,3-dione-disodium salt (CNQX, a non-NMDA receptor blocker), and picrotoxin (PIC, a GABAA receptor blocker) were purchased from Sigma-Aldrich, and L-2-amino-5-phosphonopentanoic acid (APV ...
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bioRxiv - Neuroscience 2022Quote: ... and 2% (v/v) of human serum (Sigma-Aldrich, cat. #H4522). BBB working medium was used for co-culture and assembloids ...
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bioRxiv - Immunology 2020Quote: ... cells were blocked with 2% human AB serum (Sigma-Aldrich, UK) for 10 minutes on ice and then stained with the relevant APC-labelled mAb or hIgG1 isotype (BioInvent ...
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bioRxiv - Synthetic Biology 2023Quote: ... Stock solutions of fluorescent protein (20 mg/mL and 0.2 mg/mL) and total RNA from torula yeast type VI (2 mg/mL and 0.02 mg/mL) (Sigma-Aldrich #R6625-25G) were prepared in 50 mM HEPES-NaOH ...
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bioRxiv - Bioengineering 2020Quote: ... Negatively selected human monocytes (Astarte Biologics) were suspended in RPMI 1640 supplemented with 2% human AB serum (Sigma-Aldrich), 1% GlutaMax (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: The nicotinic acetylcholine receptor (nAChR) blocker mecamylamine and GABAA receptor blocker picrotoxin (PTX) were obtained from Sigma Aldrich (Cat#: M9020, P1675 respectively). The voltage-gated sodium channel blocker tetrodotoxin (TTX ...
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bioRxiv - Neuroscience 2019Quote: ... the membranes were incubated with guinea pig antibody to D1 receptors (1:400; Alomone, Jerusalem, Israel) and rabbit antibody to D2 receptors (1:500; Millipore, Burlington, MA), washed ...
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bioRxiv - Cell Biology 2019Quote: ... type IV (Sigma). After 1 h of incubation ...
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: ... while HEK293 and A549 cells were cultured in DMEM (Sigma). Both were supplemented with 10% foetal calf serum (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: Hela and HEK293 cells were maintained in DMEM (Sigma-Aldrich) supplemented with 10 % fetal bovine serum (PAN Biotech) ...
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bioRxiv - Neuroscience 2020Quote: HEK293 cells were cultured in high glucose DMEM (Sigma-Aldrich) containing 10% FBS (HyClone) ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293 cell lines were cultured with MEM medium (Sigma-Aldrich) supplied with FBS (Funakoshi) ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were grown in Dulbecco’s Modified Eagle’s Medium (Sigma) supplemented with 10% fetal bovine serum ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were incubated in culture with 1.5% DMS (Sigma) at room temperature for 7 minutes ...
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bioRxiv - Neuroscience 2023Quote: HEK293 cells were cultured on poly-D-lysine (Sigma-Aldrich) coated glass coverslips ...
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bioRxiv - Cancer Biology 2023Quote: HEK293 derived-nsEVs were resuspended with dextran-FITC (Sigma-Aldrich) or seed saporin (SAP ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293 T-REx cells were treated with RocA (Sigma– Aldrich) at a concentration of 0.3 μM or 3 μM and incubated for 30 min before cell lysis ...
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bioRxiv - Genomics 2020Quote: Twenty-four well plates were coated with: human or rat tail collagen type I (88 ng per well; Sigma-Aldrich), human collagen type III (88 ng per well ...