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Citations for Millipore Sigma :
51 - 100 of 474 citations for Ebola Virus Secreted Glycoprotein sGP Bundibugyo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... 150 μl of 0.75mg/ml myelin oligodendrocyte glycoprotein 33 to 55 peptides (MOG35–55) mixed with Complete Freund’s Adjuvant (FS8810; Sigma-Aldrich) was injected subcutaneously into the tail base of 7 to 11-week-old female C57Bl/6 mice ...
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bioRxiv - Molecular Biology 2021Quote: ... a sample containing 10 μg of the spike glycoprotein was buffer exchanged with 50 mM ammonium citrate buffer (pH 6.5) using a 50-kDa MWCO filter (Millipore). The resulting buffer-exchanged sample was alkylated with a 20-fold molar excess of 4-vinylpyridine in the dark for one hour at room temperature to cap free cysteine residues ...
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bioRxiv - Neuroscience 2022Quote: ... Mice were immunized with 200 µg of myelin oligodendrocyte glycoprotein 35-55 (MOG35–55; MEVGWYRPFSRVVHLYRNGK) in incomplete Freund’s adjuvant (IFA; Sigma) supplemented with 8 mg/ml Mycobacterium tuberculosis H37Ra (Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were immunized with 200 µg of myelin oligodendrocyte glycoprotein 35-55 (MOG35–55; MEVGWYRPFSRVVHLYRNGK) in incomplete Freund’s adjuvant (IFA; Sigma) supplemented with 8 mg/mL Mycobacterium tuberculosis H37Ra (Fisher) ...
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bioRxiv - Biochemistry 2023Quote: Purified SARS-CoV-2 spike glycoproteins were exchanged to water using Microcon Ultracel PL-10 centrifugal filter (Millipore Sigma). Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... For each 10 cm dish, 1 ml virus inoculum was prepared in virus growth media (VGM; containing DMEM, 0.2% bovine serum albumin (Sigma; Cat no. #A8412), 25 mM N-(2-hydroxyethyl ...
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bioRxiv - Microbiology 2020Quote: ... Aggregated virus was removed by a 0.22µm filter (Millipore). Sub-confluent monolayer of MEFs cultured in DMEM ...
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bioRxiv - Physiology 2020Quote: ... Virus was filtered with a 0.45mm OVDF filter (Millipore). Supernatants were collected and purified using PEG-it (System Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... supernatant containing virus was filtered with 0.22μm filters (Millipore) and store at −80◻ in aliquots.
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bioRxiv - Cancer Biology 2023Quote: ... adherent cells were infected with COL7A1 virus (Sigma Aldrich) (1–5 MOI (multiplicity of infection) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the virus was concentrated using polyethylglycol (Sigma, P4338). Cells were infected in medium containing 5 μg ml−1 polybrene (Millipore ...
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bioRxiv - Biochemistry 2019Quote: ... ∼500-ml cell suspension containing the secreted IgG was clarified by centrifugation and subsequent filtration through a 0.45 µm HAWP membrane (Merck Millipore). The filtrate was mixed with an equal volume of binding buffer (20 mM Na2HPO4/NaH2PO4 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were assayed for secreted chemokines and cytokines using the Milliplex 38-plex Human Cytokine/Chemokine Assay (Millipore Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The rehydrated slides were blocked with 2% horse-serum followed by incubation with primary antibodies (Mouse anti-S-glycoprotein antibody, 1:200 dilution, Millipore, Cat # MAB5676 ...
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bioRxiv - Neuroscience 2024Quote: ... using a subcutaneous injection of 200mg of MOG peptide (Myelin Oligodendrocyte Glycoprotein Peptide Fragment 35 to 55; from Charité) emulsified in complete Freund’s adjuvant (from Sigma-Aldrich) that contained 200mg of Mycobacterium tuberculosis H37RA (from Difco) ...
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bioRxiv - Microbiology 2021Quote: Cytokines and chemokines secreted by HNO-ALI cells were measured and analyzed using Milliplex cytokine/chemokine magnetic bead panel (Millipore) according to the manufacturer’s instructions ...
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Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: The amount of Shh secreted by mouse pancreatic tumor cells was quantified by using the Mouse Shh-N ELISA Kit (#RAB0431, Millipore), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and secreted proteins in media were harvested after 120 hours and cleared using a 0.45-µm PVDF vacuum filter (Sigma Millipore). Each His-tagged protein in media was captured with Ni-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... Cytokines secreted by primary CF-HBECs were analyzed by the MILLIPLEX MAP Human Cytokine/Chemokine 48-Plex cytokine assay (Millipore). Cytokines were measure in the basolateral solution only since CF-HBEC were grown in air-liquid interface culture and there was insufficient apical fluid to measure cytokines.
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bioRxiv - Microbiology 2021Quote: ... Virus stocks were stored in aliquots containing 10% sucrose (Sigma) at −150°C ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... Input virus was treated with benzonase (500U/mL, Sigma-Aldrich) before cell exposure to reduce nucleic acid contamination in the viral stock ...
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bioRxiv - Neuroscience 2021Quote: ... Concentration of virus was repeated on smaller Amicon Ultracels (Millipore) until desired high titer was reached ...
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bioRxiv - Cell Biology 2021Quote: ... with 60 μg of Tobacco etch virus protease (Sigma-Aldrich) added in the dialysis cassette to cleave the histidine tag ...
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bioRxiv - Microbiology 2020Quote: ... virus was preincubated with the compound (human (Sigma-Aldrich #L8402) or chicken (Sigma-Aldrich #L4919 ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μl of virus suspension and Polybrene (hexadimethrine bromide, Sigma) at a final concentration of 8 μg/ml were added ...
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bioRxiv - Microbiology 2023Quote: ... virus was filtered using a millipore filter (Millipore Sigma, SE1M179M6). On day of infection ...
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bioRxiv - Microbiology 2023Quote: ... virus was treated with alpha-1-antitrypsin (Sigma-Aldrich, SRP6312) at the concentration noted ...
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bioRxiv - Plant Biology 2023Quote: ... and then the tobacco etch virus (TEV) protease (Sigma-Aldrich) was added to remove the purification tags ...
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bioRxiv - Microbiology 2023Quote: ... Virus propagation was performed in VeroE6 cells in DMEM (Sigma) supplemented with 2% fetal calf serum (Logan) ...
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bioRxiv - Microbiology 2023Quote: Swabs were stored in virus cultivation medium (Sigma-Virocult®). Archived swabs and organ samples were kept at-70 °C until final analyses ...
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bioRxiv - Bioengineering 2019Quote: ... the presence of structures containing a high proportion of carbohydrate macromolecules (glycogen, glycoprotein and proteoglycans) was investigated with Periodic Acid Schiff staining (Sigma-Aldrich) as described in the protocol.
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bioRxiv - Microbiology 2021Quote: ... The cells were transfected with 15 μg of a glycoprotein expressing vector using PEI (PEI, 1 mg/ml in H2O, Sigma-Aldrich). Twenty-four hours post transfection ...
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bioRxiv - Microbiology 2022Quote: ADCP assays were performed using ExpiCHO-S cells transiently transfected with SARS-CoV-2 spike glycoproteins (Wuhan D614, BA.1, or BA.2) and labelled with PKH67 (Sigma Aldrich) as targets ...
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bioRxiv - Molecular Biology 2021Quote: ... media was collected and used to measure secreted Cxcl1 protein on an ELISA plate according to the KC/Cxcl1 ELISA kit manufacturer’s instructions (Sigma-Aldrich #RAB0117). Absorbance values at 450 nm were measured with a Tecan plate reader ...
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bioRxiv - Molecular Biology 2021Quote: ... The virus was propagated on VeroE6-TMPRSS2 cells using DMEM (Sigma) supplemented with 2% (v/v ...
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bioRxiv - Bioengineering 2021Quote: ... virus was harvested and purified using an iodixanol gradient (Optiprep, Sigma). For our 7-variant pool ...
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bioRxiv - Neuroscience 2021Quote: ... Virus or Tracer was loaded into mineral oil (Sigma-Aldrich, M3516)-filled glass pipette (WPI ...
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bioRxiv - Microbiology 2021Quote: ... virus was concentrated using 100,000 MW cut-off Centricon concentrators (Millipore).
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bioRxiv - Immunology 2019Quote: ... thawed virus was lysed in Triton-X (Sigma; 9002-93-1) and used for p24 measurement at the UCLA CFAR Virology core ...
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bioRxiv - Neuroscience 2021Quote: ... This concentrated virus solution was filtered (0.22 μm Millex, Merck Millipore) and stored at 4 °C until further use ...
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bioRxiv - Cell Biology 2019Quote: ... we added concentrated virus and 5 μg/ml polybrene (Sigma-Aldrich) to MCF10A cells in growth media ...
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bioRxiv - Immunology 2022Quote: ... virus supernatant was filtered through a 0.44 um PVDF membrane (Millipore), incubated with Lipofectamine 3000 and PLUS reagent as above ...
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bioRxiv - Microbiology 2023Quote: ... the virus containing medium plus 8 µg/ml polybrene (Sigma #H9268) and the infected 293T cells were co-cultured with iSLK cells ...
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bioRxiv - Cell Biology 2023Quote: ... the virus was mixed with fully supplemented DMEM (Sigma-Aldrich, D6429) and 8 μg/ml Polybrene (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... was expressed and secreted from a stable S2 cell line (ExpreS2ion Biotechnologies) in EX-CELL® 420 Serum Free Medium (Sigma Aldrich) (Wright et al. ...
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bioRxiv - Microbiology 2023Quote: ... we also stained cells expressing wild-type and mutant EEEV glycoproteins with a mouse anti-EEEV E1 antibody clone 1A4B.6 (Sigma-Aldrich Cat#: MAB8754) or a mouse IgG2b isotype control (BD Biosciences Cat# ...
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bioRxiv - Bioengineering 2024Quote: One gram of mucin glycoprotein from bovine submaxillary gland was treated with 1U neuraminidase from Vibrio cholerae (Sigma-Aldrich, Saint Louis, MO, USA) in sodium acetate buffer 0.05 M (pH 5 ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with polybrene (TR-1003-G ...
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bioRxiv - Genetics 2021Quote: ... Increasing amounts of virus and 8 μg/ml polybrene (hexadimethrine bromide, Sigma) were added to each well ...
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bioRxiv - Microbiology 2020Quote: ... The virus was propagated once in Vero E6 cells in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...