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Citations for Millipore Sigma :
501 - 550 of 5241 citations for Dog Atrial Natriuretic Peptide ANP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Structure of the Human Signal Peptidase Complex Reveals the Determinants for Signal Peptide CleavagebioRxiv - Biochemistry 2020Quote: ... 50 mM biotin were replaced with 200 μg/mL 3x FLAG peptide (Sigma-Aldrich). Samples were buffer-exchanged into wash buffer containing DTT (dilution factor 1:400 ...
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bioRxiv - Molecular Biology 2019Quote: ... and proteins were eluted with Wash Buffer containing 0.2 mg/ml 3xFLAG peptide (Sigma). CSB proteins were further purified by gel filtration (Superdex 200 Increase ...
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bioRxiv - Cell Biology 2019Quote: ... the beads were eluted in 30µl 300 µg/ml 3XFLAG peptide (Sigma-Aldrich, #F4799) at 4°C for 45mins ...
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bioRxiv - Developmental Biology 2019Quote: ... A standard set of phosphopeptides (4 pmol/peptide sample, MS PhosphoMix1 Light, Sigma, MSPL1) were spiked into each sample ...
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bioRxiv - Immunology 2021Quote: ... pulsed or not with 1 or 10 μg/ml OVA peptide 323–339 (Sigma) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... beads were incubated with 200 μg/ml 3x FLAG peptides (Sigma-Aldrich, F4799-4MG) for 2 hrs at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The reduced peptide was alkylated with 35 μL of 1.5 M iodoacetamide (Sigma-Aldrich) and incubated in the dark at room temperature for 15 minutes ...
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bioRxiv - Cell Biology 2019Quote: ... Complexes were eluted with buffer #2 supplemented with 150 ug/ml 3xFLAG peptide (Sigma) for 1 hr at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... peptides were dialyzed with 500 - 1200 MWCO dialysis tubing (Sigma-Aldrich, St. Louis, MO) against DI water ...
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bioRxiv - Bioengineering 2021Quote: ... The crude peptides were the precipitated in ice cold diethyl ether (Et2O; Sigma-Aldrich), collected by centrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... the peptides were purified and desalted via ZipTip C18 columns (Millipore, Burlington, MA, USA). The digests were analyzed via nanoLC-MS/MS in a Thermo Scientific Q-Exactive Mass Spectrometer coupled to a nanoHPLC EASY-nLC 1000 (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Peptide was stored at 10 mg/ml in 100% dimethyl sulfoxide (DMSO; Sigma-Aldrich) at −20°C.
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bioRxiv - Molecular Biology 2021Quote: Primary rabbit antibodies used in this study were anti-calmodulin binding peptide (CBP; Millipore), anti-Glucose-6-phosphate dehydrogenase (G6PDH ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies used were rabbit anti-calcitonin gene-related peptide (1:5000, Sigma C8198) and rabbit anti-protein gene product 9.5 (1:500 ...
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bioRxiv - Immunology 2022Quote: ... OT-I splenocytes were activated in vitro with 2 μg of SIINFEKL peptides (Sigma) in the presence of recombinant human IL-2 (50 U/ml ...
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bioRxiv - Biochemistry 2022Quote: ... then eluted in 100 mM elution buffer + 1 mg/ml 3× Flag peptide (Sigma) at 4°C for 30 min.
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bioRxiv - Neuroscience 2022Quote: ... lyophilized peptides were dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP, Cat# 52517, Millipore Sigma) to ensure that the starting material was in a homogenous ...
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bioRxiv - Physiology 2023Quote: ... Samples containing 4 μg of peptides were de-salted using C18 Zip-tips (Millipore) and resuspended in 20 μl of 2.5 % (v/v ...
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bioRxiv - Immunology 2022Quote: Antigen mixture for immunization was prepared by mixing OVA(323–339) peptide (Sigma-Aldrich) or NP-OVAL (Ovalbumin ...
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bioRxiv - Neuroscience 2022Quote: ... the complex was eluted using 0.15 mg/ml 3×FLAG peptide (Millipore Cat# F4799) in GDN buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting peptide mixture was desalted using C18-Zip tips (EMD Millipore, Billerica, MA), speed-vacuumed to dryness and suspended in 0.1% formic acid for injection into ...
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bioRxiv - Neuroscience 2023Quote: ... The precipitated sample were eluted with 100 μg/ml 3XFLAG peptide (Sigma-Aldrich, F4799). The eluted samples were split in half for reducing (mix with 5x SDS sample buffer at 100°C for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted in two column volumes of 0.2 mg/ml 3xFLAG peptide (Sigma) and dialyzed against 50 mM HEPES pH 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 µg of peptides were further desalted with C18 ZipTips (Merck Millipore, Darmstadt, Germany) to ensure complete removal of beads.
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bioRxiv - Physiology 2023Quote: ... peptides were dissolved in 18:1:1 (by volume) of saline:Kolliphor EL (Sigma Aldrich):DMSO and administered to mice by intraperitoneal injections at a volume of 10 µl/g at the indicated doses for the indicated times ...
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bioRxiv - Molecular Biology 2023Quote: ... VCF1 C-terminal peptide stocks (150 μM) were prepared in pure DMSO (Sigma Aldrich). Threefold serial dilutions of VCF1 proteins or peptides were prepared in the SPR running buffer from the primary stocks starting from highest concentration of 300 nM (VCF1 C-terminal peptides ...
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bioRxiv - Physiology 2023Quote: ... Peptides from each of these fractions were cleaned up using C18 ZipTip (Millipore, USA). Each fraction was then analysed on a quadrupole-TOF hybrid mass spectrometer (TripleTOF 6600 ...
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bioRxiv - Cell Biology 2023Quote: ... The recovered tryptic peptides were resuspended in 0.1% trifluoroacetic acid (Sigma-Aldrich; cat. #T6508) and desalted with ZipTips C18 Pipette Tips (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were eluted using 10 mM glutathione or 0.5 mg/mL 3XFLAG peptide (Sigma) in Lysis Buffer with 2 mM DDM.
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bioRxiv - Cell Biology 2022Quote: ... Peptides were eluted in 10 µl 5 % DMSO in LC-MS water (Sigma Aldrich) in an ultrasonic bath for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were eluted twice with 2 μg/μl V5 peptide in water (Sigma-Aldrich) or 1 μg/μl 3x FLAG peptide (ApexBio ...
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bioRxiv - Synthetic Biology 2023Quote: ... peptide samples were loaded onto an Ascentis® ES-C18 Column (Sigma–Aldrich, USA) and were eluted from the column by using a 10 minute gradient from 98% solvent A (0.1 % FA in H2O ...
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bioRxiv - Immunology 2023Quote: ... and protein complexes were eluted with 150 mg/ml of 3X FLAG peptide (Sigma). Samples were diluted in 2X SDS sample buffer and analyzed with SDS–PAGE according to standard procedures ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were identified by indirect immunofluorescence using an anti-myc antibody (C3956, Sigma-Aldrich). SW13-human adrenocarcinoma vimentin-deficient cells ...
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bioRxiv - Biochemistry 2023Quote: ... 15 µg of peptide was incubated with 50 µl of 33 mM iodoacetamide (Sigma) in degassed 5% formic acid (pH 4 ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted in 10 µL 5% DMSO in LC-MS water (Sigma Aldrich) in an ultrasonic bath for 10 min.
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bioRxiv - Systems Biology 2024Quote: ... Samples containing 4 µg of peptides were de-salted using C18 Zip-tips (Millipore) and resuspended in 20 µl of 2.5 % (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... the immune complexes were eluted with 150 ng/µL 3×FLAG peptide (Sigma-Aldrich) in TBS solution for 30 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Samples were eluted in 45 µL of FLAG peptide (300 µg/mL, Millipore Sigma) in Wash Buffer plus 0.05% RapiGest (Waters Corp ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were resuspended in MS buffer containing 2% ACN in 1% Formic Acid (Sigma).
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bioRxiv - Microbiology 2024Quote: ... Bound protein was eluted with 100 µL of 1.5 mg/mL FLAG peptide (Millipore) resuspended in wash buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The TaPOR2 gene lacking the part coding a transit peptide and pET15b plasmid (Novagen) were amplified with PCR using Q5 polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were eluted with 50 µg/mL of 1x FLAG peptide (Sigma-Aldrich #F3290), concentrated using 30 kDa centricons (Millipore #UFC9030) ...
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bioRxiv - Plant Biology 2021Quote: ... washed 5 times and then eluted with 3xFLAG peptide ([0.1 mg/mL]; Sigma, Cat# F4799). The crosslinking was reversed overnight at 65 °C and purified DNA (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 150 mM NaCl) and eluted with 300 ng/L of FLAG peptide (#F4799, Sigma-Aldrich) for four hours under moderate agitation 62 ...
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bioRxiv - Microbiology 2020Quote: ... bound proteins from the pooled washed resin were eluted with 100μg of FLAG peptide (Sigma) in FLAG wash buffer ...
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bioRxiv - Genomics 2020Quote: ... The beads were washed extensively and were eluted with 100 μg/ml 3xFLAG peptide (Sigma). The eluates were incubated with TRIzol reagent followed by isopropanol precipitation and DNase I digestion (Fermentas) ...
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bioRxiv - Molecular Biology 2021Quote: ... The HA-tagged PGBD1 was eluted by 500 μl 100 μg/ml HA-peptide (Sigma) in 100 mMNaCl ...
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bioRxiv - Developmental Biology 2019Quote: ... Peptides were dissolved in a small amount of 0.1% trifluoroacetic acid (302031, Sigma-Aldrich; TFA), followed by purification with the use of ZipTip (MA01821 ...
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bioRxiv - Biochemistry 2019Quote: ... The native proteins without signal peptides were expressed in BL21(DE3) Gold competent cells (Novagen). The gene encoding the LtgA deletion mutant lacking the alpha helix 503(ATAREIAGKIGMD)513 was chemically synthesized by ProteoGenix ...