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Citations for Millipore Sigma :
1 - 50 of 4804 citations for Dnp pro leu gly cys me his ala D arg nh2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... and Des4 was measured using a fluorogenic substrate Dnp-Pro-Leu-Gly-Leu-Trp-Ala-D-Arg-NH2 (Sigma-Aldridge, USA) in an enzyme activity buffer (50 mM HEPES ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and MCA-Lys-Pro-Leu-Gly-Leu-Dpa(DNP)-Ala-Arg-NH2 (MMP substrate FS-6) was from Sigma-Aldrich. The stock solutions of the enzyme inhibitors and fluorogenic substrates were prepared according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... and activity was measured by the cleavage of the fluorogenic MMP Substrate MCA-Lys-Pro-Leu-Gly-Leu-DNP-Dpa-Ala-Arg-NH2 (Sigma-Aldrich). Normalized velocities were fitted in MATLAB Curve Fitting Toolbox (MathWorks ...
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bioRxiv - Cancer Biology 2023Quote: ... Where indicated an RGD peptide (sequence - Acetyl-Cys-Doa-Doa-Gly-Arg-Gly-Asp-Ser-Pro-NH2; Doa = 8-amino-3,6-dioxaoctanoic acid, Millipore Sigma) was added to the cell suspension at a concentration of 0.5 mM ...
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bioRxiv - Cell Biology 2024Quote: ... cells were treated with 200μΜ Gly-Arg-Gly-Asp-Ser-Pro (GRGDSP) or Gly-Arg-Ala-Asp-Ser-Pro (GRADSP) peptides (Sigma-Aldrich, Cat: SCP0157, SCP0156) for 6hrs before peptide removal by pelleting the cells and resuspending them in fresh DMEM medium ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM N-Succinyl-Ala-Ala-Pro-Leu-p-nitroanilide (Sigma S8511) dissolved in 100 mM MOPS pH 7.3 ...
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bioRxiv - Neuroscience 2024Quote: Amino acids (Ala, Arg, His, Phe, Ser, Trp; Sigma) were prepared as 100x stock solutions in double-distilled water ...
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bioRxiv - Cell Biology 2020Quote: ... and an RGD peptide (Gly-Arg-Gly-Asp-Ser/GRGDS, Sigma-Aldrich) for 20 min at room temperature before the cells were added to the wells for incubation for 3 hours at 37°C.
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bioRxiv - Neuroscience 2023Quote: ... (D-Ala(2)-mephe(4)-gly-ol(5))enkephalin (DAMGO, Sigma-Aldrich, Cat. No. E7384) at 50 µM or lipopolysaccharide (LPS ...
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The ATP-dependent protease ClpYQ degrades cell division proteins DivIVA and Mbl in Bacillus subtilisbioRxiv - Biochemistry 2024Quote: ... and ClpYQ activity was confirmed through hydrolysis of the fluorogenic Cbz-Gly-Gly-Leu-AMC (Millipore Sigma) substrate.
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bioRxiv - Neuroscience 2021Quote: ... amino acids (His, Ser, Ala, Trp; Sigma) were diluted to a final concentration of 10-4 M and bile acid (TDCA ...
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bioRxiv - Microbiology 2024Quote: ... Ecotin or PIC (positive control) and N-Methoxysuccinyl-Ala-Ala-Pro-Val-AMC (Sigma) 20µM as substrate ...
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bioRxiv - Evolutionary Biology 2023Quote: ... D-alanine (D-ala, Sigma-Aldrich #A7377), D-cysteine (D-cys ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated with increasing concentrations (0 to 120 μg/ml) of a reverse sequence control peptide (Ser-Asp-Gly-Arg-Gly/SDGRG, Sigma-Aldrich) and an RGD peptide (Gly-Arg-Gly-Asp-Ser/GRGDS ...
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bioRxiv - Cell Biology 2021Quote: ... SC –leu-trp plates and SC –leu-trp-his + 10mM 3-aminotriazole (Sigma) plates ...
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bioRxiv - Biochemistry 2024Quote: ... and MeOSuc-Ala-Ala-Pro-Val-pNA (324696) for neutrophil elastase (Millipore Sigma, Burlington, MA, USA).
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bioRxiv - Evolutionary Biology 2023Quote: ... D-leucine (D-leu, Sigma-Aldrich #855448), D-cycloserine (DCS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... D-cysteine (D-cys, Sigma-Aldrich #30095), D-2-aminobutyric acid (D2ABA ...
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bioRxiv - Microbiology 2020Quote: ... D-ala-D-ala dipeptide formation in the reaction catalysed by Ddl was identified by comparison with commercially available D-ala-D-ala (Sigma). 2 ...
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bioRxiv - Immunology 2021Quote: Elastase activity was determined by the chromogenic substrate N-Methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide (M4765, Sigma-Aldrich, USA). Supernatants were used for measuring elastase activity ...
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bioRxiv - Molecular Biology 2021Quote: ... SD-Leu-Trp-His+1mM 3-AT (3-aminotriazol, Sigma) and SD-Leu-Trp-His-Ade (Formedium).
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bioRxiv - Biochemistry 2021Quote: ... including an N-terminal TEV site followed by a Gly-Gly-Gly sequence and a Gly-Ser-GLy-Ser-GLy spacer (Met-Glu-Asn-Leu-Tyr-Phe-Gln-Gly-Gly-Gly-Gly-Ser-GLy-Ser-Gly-BLS) was assembled and cloned in the pET11a vector (Novagen). The construct was checked by sequencing ...
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bioRxiv - Systems Biology 2024Quote: ... on SC(MSG)-Leu/Arg/Lys/Ura + canavanine/thialysine plates (50 mg/l canavanine (Sigma-Aldrich, C1625), 50 mg/l thialysine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Serial dilutions of the concentrated supernatants were then incubated with human neutrophil elastase (MilliporeSigma) in the presence of methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide (Millipore Sigma). To quantify bioactivity colorimetric change was quantified using a 96 well plate reader set at 405nm (54).
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bioRxiv - Cell Biology 2021Quote: ... To enhance H2O2 levels D-Ala (Sigma-Aldrich, #A7377) (10 mM ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting transformants were spotted on SD (−Leu, −Trp) and SD (−Trp, −Leu, −His) selection media containing different concentration of 3-amino-1,2,4-triazole (Sigma, A8056) as previously reported (Putarjunan et al. ...
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bioRxiv - Biochemistry 2023Quote: ... The chromogenic substrate Gly-Pro-pNA and the POP inhibitor Z-Pro-prolinal (ZPP) were ordered from Sigma-Aldrich. Follower and leader (clasp domain ...
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bioRxiv - Neuroscience 2024Quote: ELANE catalytic activity was determined using the synthetic substrate N-methoxysuccinyl-Ala-Ala-Pro-Val p-nitroanilide (M4765, Sigma-Aldrich, St. Louis, MO). In brief ...
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bioRxiv - Biochemistry 2024Quote: ... MG-132 (Z-Leu-Leu-Leu-al, Sigma-Aldrich) was dissolved in DMSO (10 mM stock ...
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bioRxiv - Molecular Biology 2020Quote: ... 3-15 µM MG132 (Z-Leu-Leu-Leu-al, Sigma) was added to the media for the indicated hours prior to cell lysis/fixation ...
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bioRxiv - Biochemistry 2021Quote: ... and Z-Leu-Leu-Leu-al (475.62 Da; Sigma C2211). Detergents were purchased from Sigma Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Glycine-Glycine (Gly-Gly, Sigma-Aldrich, G1002-25G) 150 mM (24 h ...
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bioRxiv - Neuroscience 2021Quote: ... Gly (Sigma), DA (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... Haploid cells were selected in SD-Leu (for Mat alpha selection) and -Arg-Lys with toxic amino-acid derivatives canavanine and thialysine (Sigma-Aldrich) to select against diploids ...
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bioRxiv - Molecular Biology 2024Quote: ... Glutaryl-Ala-Ala-Phe-4-methoxy-β-naphthylamide (Sigma). For the basal peptidase rate ...
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bioRxiv - Molecular Biology 2021Quote: ... SC-leu / SC-leu + 0.4mM CuSO4 (Sigma) or SC-leu / SC-leu + 0.25 CuSO4 and incubating the plates at 30°C for several days ...
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bioRxiv - Biochemistry 2024Quote: ... while MeSO2-D-CHA-But-Arg-pNA was from Sigma (Darmstadt, Germany). Mpro FRET-based substrate 5-FAM-AVLQSGFRK(DABCYL)K (ProteoGenix ...
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bioRxiv - Neuroscience 2020Quote: ... Gly (Sigma-Aldrich), NE (Tocris) ...
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bioRxiv - Microbiology 2021Quote: ... β-Ala (Sigma, 146064) or stable isotope labeled metabolites ...
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bioRxiv - Pathology 2023Quote: ALA (Sigma-Aldrich) was dissolved in 100% DMSO and then diluted with sterile distilled water containing 0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2022Quote: ... Leu-Leu methyl ester hydrobromide (Millipore Sigma; L7393), MLSA1 (Millipore Sigma ...
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bioRxiv - Microbiology 2024Quote: The hydrolytic activity of the bacterial DPP4 enzyme was measured using Gly-Pro p-nitroanilide hydrochloride (gpPNA) (Sigma), as described for assessing hsDPP4 activity [39] ...
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bioRxiv - Molecular Biology 2024Quote: ... or SC-Leu-Trp-His +5 mM 3-AT (3-Amino-1,2,4-triazole, Sigma, 18056-25G). Plates were incubated at 30°C and imaged daily for at least three days.
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bioRxiv - Biochemistry 2022Quote: ... The ligand Ala-Ala were filtered through 0.45 µm nitrocellulose filters (Millipore). Cells were lysed in 0.1M NaOH and 1% SDS for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... 125 nM Glutaryl-Ala-Ala-Phe-4-methyl-β-naphthylamide (Sigma-Aldrich), 0.1uM Lon6 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were treated with 10μM of MG132 (Z-Leu-Leu-Leu-al; Sigma, catalog no. C2211). Cells were then harvested at 1h after MG132 treatment (corresponds to 16h post second thymidine recovery ...
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bioRxiv - Neuroscience 2024Quote: ... glycine (Gly, Sigma-Aldrich). Experiments were also performed using pressure application of aCSF in the absence of drugs to test for the artefacts or mechanicals responses ...
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bioRxiv - Microbiology 2023Quote: ... in a well volume of 100 μL of a peptide substrate that includes the nsp5 cleavage site between nsp4 and nsp5 proteins (nsp4-5) (-AVLQ ↓ SGFR[K(DNP)]K-NH2 (Millipore Sigma, Milwaukee, WI, USA; >95%). For WTWA1 ...
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bioRxiv - Cancer Biology 2020Quote: ... L-Ala (Sigma, A7627), L-Ser (Sigma ...
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bioRxiv - Immunology 2023Quote: ... lavage fluids (1/2 diluted; 100 µl) were incubated with 500 µM Gly-Pro-p-nitroanilide substrate (Sigma-Aldrich) in 200 mM Tris-HCl buffer (pH 8.3 ...