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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for DnaJ Hsp40 Homolog Subfamily C Member 10 DNAJC10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 4°C with PSD-95 primary antibodies (1:500, Millipore, MAB1598, RRID:AB_11212185) and/or rabbit anti-mCherry primary antibodies (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated with primary antibodies overnight at 4 °C (GLT-1: Millipore AB1783 at 1:4000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were incubated with monoclonal anti-(c-Myc Tag) antibody (Merck Millipore) (HeLa extracts) ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies used were rabbit polyclonal c-Fos (1:4000, Sigma-Aldrich); rat monoclonal mCherry (1:2000 ...
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bioRxiv - Immunology 2024Quote: ... Protein extracts were immunoprecipitated with a PP2A C-specific antibody (1D6; Millipore). An appropriate phosphorylated peptide (amino acid sequence K-R-pT-I-R-R ...
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bioRxiv - Developmental Biology 2019Quote: ... Tissues were blocked in 5% normal donkey serum before an overnight incubation with the primary antibody at 4°C (1:200 for ICSBP antibody, sc-365042, Santa Cruz; and 1:100 for MEF2C antibody, SAB4501860, Sigma-Aldrich). The slides were washed twice in PBS at room temperature and applied with secondary antibody diluted 1:200 in 1% BSA prepared in PBS (biotinylated anti-mouse IgG (H+L) ...
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bioRxiv - Developmental Biology 2020Quote: ... and were then incubated overnight at 4°C with primary antibody (Rabbit anti-H3P antibody, Millipore 06-570) at 1:1,000 concentration ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated overnight at 4°C with primary antibody mouse anti-Flag antibody (1:1000, Sigma). After three washes in PBS (5 min each) ...
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bioRxiv - Developmental Biology 2023Quote: ... The following primary antibodies were used for protein blotting: mouse anti-c-Myc antibody (Sigma-Aldrich, 05-724MG), mouse anti-Flag antibody (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared by centrifugation at 16,000 g for 10 min at 4 °C and then incubated with 10 µl anti-FLAG M2-Sepharose beads (Sigma) for 60 min at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... tumefaciens GV3101 strains harboring expression constructs of interest were pre-incubated at room temperature (ca. 22°C) in induction media (10 mM MES pH 5.6, 10 mM MgCl2, 150 mM acetosyringone [Sigma-Aldrich]) for 2 h before hand-infiltration into 5-week-old N ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma] ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, #MRCPRT010 Ultracel YM-10). The mixture was centrifuged at 1000 rcf for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... 4-well LabTek dishes were incubated 30 min at 37°C with 10 μg/ml fibronectin or O/N at 37°C with 20 μg/ml laminin (Sigma, L2020-1MG). RPE1 cells were then plated at low density in the dishes and imaged the next day ...
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bioRxiv - Synthetic Biology 2019Quote: ... the 6x-His Tag Polyclonal Antibody or HA Tag Polyclonal Antibody was the primary antibody and the anti-rabbit IgG-gold (10 nm) antibody (Sigma-Aldrich) was the secondary ...
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bioRxiv - Developmental Biology 2021Quote: ... or 10 μg anti-FLAG antibody (Sigma, F3165/F1804), or 10 μg anti-HA antibody (Abbkine ...
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bioRxiv - Microbiology 2022Quote: ... All antibodies were diluted in 10% horse serum (Sigma) and 0.1% saponin (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... and rotated overnight with 10 μg APOE antibody (Sigma) or IgG (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1:10 anti GIV antibody (Cat#ABT80; Millipore) in 1% BSA/PBS for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and 10 nM anti-mouse IgG-Gold antibody (Sigma) as the secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies were diluted in 10% milk solution (Sigma) in TBS and were incubated overnight at 4°C (Table 2) ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were blocked with 10% FBS in PBS for 1 h at RT and incubated overnight at 4°C with a primary anti-γH2AX antibody (05-636, EMD Millipore, 1:500 in 10% FBS-PBS). Following primary antibody incubations ...
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bioRxiv - Biophysics 2021Quote: ... and probed overnight at 4 °C with primary antibodies against pospho-Lamin A/C (Ser22) (D2B2E from CST) and vinculin (V9131 from Sigma Aldrich). After washings with 1X PBS ...
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bioRxiv - Pathology 2023Quote: ... for 30 min at 37 °C and then incubated overnight at 4°C with primary antibodies pre- conjugated with Red Oligo A (DUO86010A, Sigma-Aldrich) or Red Oligo B (DUO86010B ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were cross-linked for 10 min at 37°C in 1% formaldehyde (Sigma) followed by quenching with 125 mM glycine for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 24 h before treatment with the cytostatic Mitomycin C (10 μg/ml, Sigma, #M4287) for 3.5 h in normal medium ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... fibroblasts were proliferation-inactivated by incubation in mitomycin C (10 μg/mL, Sigma, M4287) for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were fixed for 10 minutes with cold (−20°C) methanol (Sigma-Aldrich, Singapore), blocked for 2 hours with 1% BSA (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were incubated for 10 min at 4 °C in RIPA buffer (Sigma, USA). After a centrifugation at 12,000 × g for 10 min at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were incubated at 37°C for 10 min with FastBCIP/NBT (Sigma #B5655) dissolved in MiliQ water ...
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bioRxiv - Molecular Biology 2023Quote: ... at 60° C for 30 min and alkylated with 10 mM Iodoacetamide (Sigma-Aldrich) for 10 min at room temperature in the dark ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were fixed overnight at 4°C in 10% Neutral Buffered Formalin (HT501125, Sigma) and embedded in paraffin following standard processing (45 min ...
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bioRxiv - Genetics 2023Quote: Cultured chondronoids were fixed overnight at 4 °C in 10% neutral-buffered formalin (Millipore), washed in 70% ethanol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... at 90 °C for 15 min and alkylated using 10 mM iodoacetamid (Sigma Aldrich) at 20 °C for 30 min in the dark ...
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bioRxiv - Microbiology 2024Quote: ... for 30 minutes at 37°C or with 10 mM hydrogen peroxide (Sigma-Aldrich) for 60 minutes at 37°C (52) ...
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bioRxiv - Molecular Biology 2019Quote: ... each mixed with either 10 µl of the α-FLAG antibody or 10 µl of the α-HA antibody (Sigma-Aldrich, Cat#H6908) and incubated for 30 min with constant rocking ...
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bioRxiv - Molecular Biology 2021Quote: ... BAT was then incubated for 1 hour at 37 °C before stimulation with 10 μM isoproterenol (ISO, Sigma, 54750-10-6). Samples were taken at the indicated time points ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was thawed on ice and resuspended in 50 μl SLB/c buffer (10 mM Tris-HCL pH 8, 10 mM EDTA, 1% SDS supplemented with protease inhibitor (11836170001, Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2020Quote: ... Cultures were then centrifuged at 2,500 × g for 10 min at 4°C and proteins from the supernatant were precipitated using 10% tricholoroacetic acid (TCA) with acetone (Sigma-Aldrich). 1,700 μl of TCA-acetone was added to 300 μl of culture supernatant and incubated at −20°C for one hour ...
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bioRxiv - Neuroscience 2020Quote: ... filled with pre-heated (to 37 °C) CUBIC-L solution (10% w/w N-butyldiethanolamine, 10%w/w triton X-100, in H2O, Sigma Aldrich) and kept at 37 °C under continuous shaking ...
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bioRxiv - Neuroscience 2023Quote: ... in a 10×10×5mm cryomold (Tissue-Tek #4565) and submerged in dry-ice chilled (approximately −70°C) isopentane (Sigma #M32631). Frozen embedded brain tissues were kept on dry ice until being transferred to −80°C storage.
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bioRxiv - Molecular Biology 2023Quote: ... was incubated for 2 h at 55 °C and for 10 min at 95 °C in 50 μL lysis solution (proteinase K -Sigma-Aldrich, Munich, Germany) freshly diluted 1:100 in lysis buffer (25 mM NaOH ...
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bioRxiv - Molecular Biology 2021Quote: ... 1/1000) antibodies for 2 h at 37 °C in antibody diluent solution (Duolink in situ kit, Sigma-Aldrich). The cells were then labeled with the PLA antimouse PLUS probe (DUO92001 ...
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bioRxiv - Cancer Biology 2022Quote: ... which were subsequently incubated overnight at 4 °C with primary antibodies as follows: anti-P2X7 antibody (P8232, Sigma-Aldrich) was diluted 1:300 ...
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bioRxiv - Bioengineering 2021Quote: ... and incubated at 4 °C overnight with antibodies against βIII-Tubulin (Millipore, MAB1637), MAP2 (Thermo Fisher ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies used for immunocytochemistry were: lamin A/C (1:250, Millipore MAB3211), progerin (1:250 ...
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bioRxiv - Immunology 2022Quote: ... at 4°C in the dark with anti-IFIT1/p65 antibody (Sigma-Aldrich), washed with FACs buffer ...
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bioRxiv - Pathology 2019Quote: ... sections were incubated with c-myc antibody (1:1000, rabbit polyclonal, Sigma-Aldrich).