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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... RMG-1 ARID1A-WT and ARID1A-KO cells were seeded at a density of 2.5×106 cells/well in 12-well plates and spin-fected (2000 rpm for 2 hours at 37°C) with increasing concentrations of virus in the presence of 8µg/mL polybrene (Millipore TR-003-G lot#3287963). The next day ...
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bioRxiv - Microbiology 2021Quote: ... A 2 mL volume of crude phage lysate (∼109 pfu/mL) was mixed with 25 µL MgCl2 (1M, Sigma), 0.8 µL DNase I (2000 U/ml ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were incubated for 2 h on a rotating wheel at 4°C with anti-FLAG M2 beads (Sigma). Beads were washed once with lysis buffer (without benzonase ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 ml of the soluble lysate was incubated with 30 µl of anti-FLAG M2 affinity resins (Sigma-Aldrich) for 2 h at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with u-[2H,13C,15N] labeled algal lysates (2 g/L of culture unless specified otherwise; Sigma-Aldrich) and 2H,13C D-glucose as an additional carbon and energy source ...
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bioRxiv - Microbiology 2021Quote: ... Virus stocks were stored in aliquots containing 10% sucrose (Sigma) at −150°C ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... Input virus was treated with benzonase (500U/mL, Sigma-Aldrich) before cell exposure to reduce nucleic acid contamination in the viral stock ...
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bioRxiv - Neuroscience 2021Quote: ... Concentration of virus was repeated on smaller Amicon Ultracels (Millipore) until desired high titer was reached ...
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bioRxiv - Cell Biology 2021Quote: ... with 60 μg of Tobacco etch virus protease (Sigma-Aldrich) added in the dialysis cassette to cleave the histidine tag ...
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bioRxiv - Microbiology 2020Quote: ... virus was preincubated with the compound (human (Sigma-Aldrich #L8402) or chicken (Sigma-Aldrich #L4919 ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μl of virus suspension and Polybrene (hexadimethrine bromide, Sigma) at a final concentration of 8 μg/ml were added ...
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bioRxiv - Microbiology 2023Quote: ... virus was filtered using a millipore filter (Millipore Sigma, SE1M179M6). On day of infection ...
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bioRxiv - Microbiology 2023Quote: ... virus was treated with alpha-1-antitrypsin (Sigma-Aldrich, SRP6312) at the concentration noted ...
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bioRxiv - Plant Biology 2023Quote: ... and then the tobacco etch virus (TEV) protease (Sigma-Aldrich) was added to remove the purification tags ...
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bioRxiv - Microbiology 2023Quote: Swabs were stored in virus cultivation medium (Sigma-Virocult®). Archived swabs and organ samples were kept at-70 °C until final analyses ...
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bioRxiv - Microbiology 2023Quote: ... Virus propagation was performed in VeroE6 cells in DMEM (Sigma) supplemented with 2% fetal calf serum (Logan) ...
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bioRxiv - Immunology 2019Quote: Mice were injected intraperitoneally (i.p.) with 15 mg/kg BW of LPS (Escherichia coli serotype O55:B5; Sigma-Aldrich, St Louis, MO). Mice were allocated to treatment or vehicle group ...
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bioRxiv - Neuroscience 2023Quote: ... Lipopolysaccharide (LPS serotype O55:B5, gamma-irradiated) and lipoteichoic acid (LTA; from Staphylococcus aureus) were purchased from Sigma (L6529 and L2515, respectively). Recombinant mouse granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysate was immunoprecipitated for 2 hours at 4°C with the following antibodies: rabbit anti-myc (2.5 μg, Millipore Sigma), mouse anti-HA (2.5 μg ...
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bioRxiv - Immunology 2019Quote: ... The pellets of the lysates were washed with PBS for three times and cross-linked using fresh DSS (2 mM, Sigma) at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were immunoprecipitated with 2 μg of IgG (negative control) or Smad3 antibody in coimmunoprecipitation reagents (17-195, Millipore). Semi-quantitative and quantitative PCR were performed to amplify α-SMA or Col1A promoter regions containing SBE.
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bioRxiv - Biochemistry 2021Quote: ... The second half of the clarified lysate was incubated for 2 h at 4°C with 20 μl of Anti-FLAG M2 magnetic beads (Sigma) equilibrated in FLAG wash buffer containing 20 mM HEPES pH 8.0 ...
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bioRxiv - Genomics 2022Quote: ... The lysate was mixed with 2 mL of 50% Iodixanol (prepared by mixing OptiPrep™ Density Gradient Medium (Sigma #D1556) with diluent (120 mM Tris-Cl pH=8.0 ...
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bioRxiv - Molecular Biology 2022Quote: 400 μl of cell lysate was incubated overnight at 4 °C with 2 μg of anti-Myc or anti-Flag antibody (Sigma). Protein A-Sepharose (GE ...
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bioRxiv - Cell Biology 2024Quote: ... Lysates were then diluted with 500 µL lysis buffer supplemented with protease inhibitors and 2% glyco-diosgenin (Sigma, Cat. # 850525) and incubated rotating at 4 °C for 1h ...
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bioRxiv - Neuroscience 2023Quote: P10 Nf1+/- and WT littermate male pups received a single intraperitoneal injection of 500 ug/kg lipopolysaccharide (Escherichia coli, serotype O26:B6, Sigma-Aldrich, Oakville, Canada) or a matched volume of 0.9% saline solution (sterile vehicle) ...
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bioRxiv - Molecular Biology 2021Quote: ... The virus was propagated on VeroE6-TMPRSS2 cells using DMEM (Sigma) supplemented with 2% (v/v ...
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bioRxiv - Bioengineering 2021Quote: ... virus was harvested and purified using an iodixanol gradient (Optiprep, Sigma). For our 7-variant pool ...
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bioRxiv - Neuroscience 2021Quote: ... Virus or Tracer was loaded into mineral oil (Sigma-Aldrich, M3516)-filled glass pipette (WPI ...
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bioRxiv - Microbiology 2021Quote: ... virus was concentrated using 100,000 MW cut-off Centricon concentrators (Millipore).
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bioRxiv - Immunology 2019Quote: ... thawed virus was lysed in Triton-X (Sigma; 9002-93-1) and used for p24 measurement at the UCLA CFAR Virology core ...
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bioRxiv - Neuroscience 2021Quote: ... This concentrated virus solution was filtered (0.22 μm Millex, Merck Millipore) and stored at 4 °C until further use ...
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bioRxiv - Cell Biology 2019Quote: ... we added concentrated virus and 5 μg/ml polybrene (Sigma-Aldrich) to MCF10A cells in growth media ...
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bioRxiv - Immunology 2022Quote: ... virus supernatant was filtered through a 0.44 um PVDF membrane (Millipore), incubated with Lipofectamine 3000 and PLUS reagent as above ...
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bioRxiv - Microbiology 2023Quote: ... the virus containing medium plus 8 µg/ml polybrene (Sigma #H9268) and the infected 293T cells were co-cultured with iSLK cells ...
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bioRxiv - Cell Biology 2023Quote: ... the virus was mixed with fully supplemented DMEM (Sigma-Aldrich, D6429) and 8 μg/ml Polybrene (Sigma-Aldrich ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... The remaining lysate was then incubated with 25 μL (1 μL/2 OD600 nm of cells) of monoclonal anti-FLAG M2 (Sigma #F1804) at 4°C with rotation for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed 1-2 days later and the lysates were immunoprecipitated using anti-FLAG M2 affinity resins (10 μl per plate, Sigma Inc.). TNKS antibodies were as described (No ...
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bioRxiv - Cell Biology 2019Quote: ... The sonicated lysate was incubated for 2 hours at 4 ° C with beads (Glutathione High Capacity Magnetic Agarose Beads, Sigma-Aldrich) previously washed in Rac buffer (50 mM Tris HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Pre-cleared nuclear lysate was then added to 50 μL beads conjugated with 2-4 μg primary IgG antibody: rabbit anti-APOBEC2 (Sigma, HPA017957) or rabbit IgG isotype control ...
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bioRxiv - Biochemistry 2021Quote: ... 400 μg of each lysate was diluted to 2 mg/ml with lysis buffer and incubated with 10 mM TCEP (646547, Sigma-Aldrich) for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 20 μg of the resulting protein lysates were mixed with 1 x Laemmli loading dye containing 5% 2-Mercaptoethanol (Sigma, M3148), separated on 10% polyacrylamide gel and semi-dry blotted to nitrocellulose membrane ...
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bioRxiv - Cell Biology 2023Quote: ... Each lysate was subjected to immunoprecipitation for 2 h or overnight with anti-FLAG M2 affinity agarose affinity gels (Sigma-Aldrich) or anti-HA magnetic beads (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2022Quote: Polyacrylamide gel slices containing bEV and sEV protein lysates were resolved in tris (2-carboxyethyl) phosphine (10 × 10-1 M; Sigma- Aldrich) with ammonium bicarbonate (50 × 10-1 M ...
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bioRxiv - Genomics 2023Quote: ... The lysate was benzonase (Millipore, 70664) treated for 30 min ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitation of FLAG-Myc was performed by incubating 2 mg of cell lysate with 40 µl of Anti-FLAG M2 affinity gel (Sigma-Aldrich #A2220) for 3h in a final volume of 1 ml of NHEN buffer with agitation at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... the supernatants of cell lysates were incubated with magnetic beads coated with anti-Ago 2 or control anti-IgG (Millipore, MA, USA) for 4 h at 4°C ...
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bioRxiv - Genomics 2020Quote: ... was used to determine protein levels of each sample in which 2 mg of nuclear lysates was incubated overnight with 50 uL of ANTI- FLAG M2 magnetic beads (Sigma Cat# M8823) at 4°C or for 2 hours with 20 uL of GFP-Trap magnetic beads (Chromotek ...
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bioRxiv - Biochemistry 2023Quote: ... Immunoprecipitation was carried out by mixing 500-800 µg of whole cell lysate and 1-2 µg of anti-Flag M2 antibody (Sigma Millipore, #F3165), 1-2 µg of anti-c-Myc antibody (Santa Cruz Biotechnology ...