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Citations for Millipore Sigma :
201 - 250 of 10000+ citations for Dengue Virus Serotype 2 DIII envelope protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The CbAgo protein was expressed in E.coli Bl21(DE3) Rosetta™ 2 (Novagen). Cultures were grown at 37 °C in LB medium containing 50 μg ml−1 kanamycin and 34 μg ml−1 chloramphenicol till an OD600 nm of 0.7 was reached ...
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bioRxiv - Biophysics 2019Quote: ... The CbAgo protein was expressed in E.coli Bl21(DE3) Rosetta™ 2 (Novagen). Cultures were grown at 37°C in LB medium containing 50μa.g ml-1 kanamycin and 34μg ml-1 chloramphenicol till an ODóOOnm of 0.7 was reached ...
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bioRxiv - Biochemistry 2019Quote: ... and 1% NP-40 containing protein phosphatase inhibitor cocktail 1 and 2 (Sigma) and protease inhibitors (Sigma)] ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... antimicrotubule-associated protein 2 (MAP2) Alexa Fluor 647 (1:500, NB120-11267AF647; Millipore). Cell nuclei were stained by using Hoescht 33342 (2 μg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were subsequently incubated for 2 h with Protein-G–Sepharose (Sigma-Aldrich). After 3 washes with lysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
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bioRxiv - Genetics 2021Quote: ... Each ORF cloned into the pLVX-EF1a-IRES-PURO/eGFP lentiviral expression vector was added to lentiviral packaging and envelope vectors (Sigma-MISSION-gag-pol and the envelope Sigma-MISSION-VSV-G (Cat ...
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bioRxiv - Molecular Biology 2022Quote: Mammalian lentiviral particles harboring sgRNA-encoding plasmids or cDNA-encoding plasmids were co-transfected with the psPAX2 envelope and VSV-G packaging plasmids into actively growing HEK-293T cells (ATCC) using Xtremegene-HP (Sigma) transfection reagent as previously described (Sarbassov et al. ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of protein lysate were incubated for 3 h with 2 μg of α-FLAG M2® antibody (Sigma-Aldrich) pre-coupled to 20 μl of rotein G Dynabeads® (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with polybrene (TR-1003-G ...
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bioRxiv - Genetics 2021Quote: ... Increasing amounts of virus and 8 μg/ml polybrene (hexadimethrine bromide, Sigma) were added to each well ...
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bioRxiv - Microbiology 2020Quote: ... The virus was propagated once in Vero E6 cells in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Systems Biology 2020Quote: ... Virus was concentrated using Amicon Ultra-15 Centfigiual Filter Concentrators (Millipore UFC910008). Virus was titered by plaque assay ...
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bioRxiv - Microbiology 2021Quote: ... influenza virus NP (clones A1 and A3, MAB8251, Sigma-Aldrich, 1:100) and anti-mouse IgG AlexaFluor-488 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... virus-containing supernatants were filtered through a 0.45 μm membrane (Merck Millipore). The amount of virus in the supernatants was quantified by RT assay ...
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bioRxiv - Cell Biology 2022Quote: ... Virus amplification was performed in Sf9 TriEX cells (Novagen / Merck #71023-3) grown in TriEx media (Novagen / Merck #71022) ...
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bioRxiv - Immunology 2022Quote: ... virus particles were precipitated by adding 50% (w/v) PEG6000 (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was backfilled into a pipette of mineral oil (M5904, Sigma-Aldrich). We injected 400nl virus into the identified C2 barrel column through a single injection site over 4 min at 300μm below pia ...
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bioRxiv - Immunology 2020Quote: Inactivated virus was concentrated using Amicon Ultra Centrifugal Filter units (Millipore Sigma) at 100k cutoff ...
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bioRxiv - Microbiology 2020Quote: ... Unabsorbed virus were removed and cells were overlaid with MEM (Sigma-Aldrich) containing 0.65% agarose (Nacalai tesque ...
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bioRxiv - Neuroscience 2023Quote: ... The virus-infected GL261 cells were trypsinized with 0.25% trypsin (Sigma-Aldrich) 72 hours after infection ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with 4-fold volume of fresh media containing 12.5 µg/ml polybrene (TR-1003-G ...
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bioRxiv - Cell Biology 2023Quote: ... Virus was then filtered with a 0.45 µm PVDF filter (SLHV013SL, Millipore) and mixed with 4-fold volume of fresh media containing 12.5 µg/ml polybrene (TR-1003-G ...
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bioRxiv - Cancer Biology 2023Quote: ... An empty TRC1 lentiviral construct was used as the Control virus (Sigma).
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bioRxiv - Cancer Biology 2023Quote: ... The virus was concentrated using Amicon Ultra filters (Millipore cat. no. UFC910024) until they have about 1.5ml of concentrated virus ...
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bioRxiv - Cell Biology 2023Quote: ... where cells and virus were mixed with polybrene (Millipore, TR-1003-G) in antibiotic-free media at 2 M cells per well of a 12-well plate ...
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bioRxiv - Cell Biology 2023Quote: ... where cells and virus were mixed with polybrene (Millipore TR-1003-G) in antibiotic-free media at 2 M cells per well of a 12-well plate ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transduced with virus along with 10 μg/mL polybrene (Sigma). Following transduction ...
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bioRxiv - Genomics 2024Quote: ... virus-containing supernatant was filtered through a 0.45 μm filter (Merck Millipore), aliquoted ...
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bioRxiv - Microbiology 2020Quote: ... cultured for 24 h at 37°C with 5% CO2 and transduced with pseudoviruses harboring VSV-G or S-SARS-CoV-2 proteins or lacking the fusion protein (∆Env) in the presence of polybrene (4 µg/ml; Sigma-Aldrich, Poland) and HTCC-77 (100 μg/ml ...
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bioRxiv - Biochemistry 2022Quote: The expression vector pGEX-6P-1-GST-FLAG containing the relevant fusion protein and fluorescence protein was transformed into Rosetta™ 2 (pLysS) host strains (Novagen; 71403). A single colony was inoculated in 5.5 mL LB broth (Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 70µg total protein per sample was reduced with tris-2-carboxyethyl phosphine (TCEP, Sigma) for 1 hour at 60°C at a final concentration of 5mM ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were then reduced by 10 mM tris(2-carboxyethyl)phosphine (TCEP, Millipore Sigma) for 30 min at 37 °C ...
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bioRxiv - Immunology 2019Quote: ... mouse anti-MAP2 (microtubules associated protein 2) antibody (Cat. MAB378, Millipore, Boston, MA, USA), anti-ZIKV-E antibody (Cat ...
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bioRxiv - Neuroscience 2022Quote: ... Protein was diluted in Laemmli sample buffer with 5% 2-Mercaptoethanol (Sigma-Aldrich M6250) and denatured for 5 mins at 95°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... 6 μg of purified protein was mixed with 2 mM of acetyl phosphate (Sigma) in 100 μl of phosphorylation buffer (50 mM Tris-HCl pH 7.6 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein pellets were solubilised in 30 μl of 2 M Tris pH 8.0 (Sigma) / 70μl 3X SDS-PAGE loading buffer (60 mM Tris pH 6.8 (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: Recombinant protein expression was performed using Eschericia coli strain Rosetta™ 2 (DE3) (Novagen) (Merck Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl) phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then reduced by 10 mM tris(2-carboxyethyl)phosphine (TCEP, Millipore Sigma) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... these were supplemented with 2 ng/ml recombinant human FGF basic protein (Sigma-Aldrich), 15 mM sodium bicarbonate ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were eluted twice with 2 μg/μl V5 peptide in water (Sigma-Aldrich) or 1 μg/μl 3x FLAG peptide (ApexBio ...
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bioRxiv - Biophysics 2023Quote: ... GST-tagged Elf1 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Glutathione Sepharose 4 Fast Flow resin (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich) and alkylated with 10 mM chloroacetamide (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were reduced using 5 mM tris (2-carboxyethyl) phophine hydrochloride (TCEP) (Sigma; 75259) for 10 min at 95° C and alkylated using 10 mM 2-iodoacetamide (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK-293T cells were cotransfected with gene-of-interest (GOI)-containing vectors with doxycycline dependent promoter and components of 2nd generation packaging vectors: pPAX2 packaging vector and pMD2G envelope vector using linear PEI 25000 (SIGMA-ALDRICH). 48 h post-transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... Retroviruses were packaged by co-transfection of Gag-Pol expressing 293 T cells with expression constructs and envelope vectors (VSV-G) using polyethylenimine (PEI; Sigma-Aldrich). Following transduction with Ccl2 or control Empty constructs ...