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Citations for Millipore Sigma :
451 - 500 of 1956 citations for DNA Sequencer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Anti-DNA-RNA hybrid antibody S9.6 (MABE1095, Sigma-Aldrich), anti-NRBP1/2 antibody (21549-1-AP ...
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bioRxiv - Microbiology 2022Quote: ... the DNA template was digested with DNase I (Sigma) and the RNAs were purified with phenol-chloroform alcohol isoamyl ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... DNA was stained with Hoechst 33258 (1:1000, Sigma). Coverslips were mounted in Mowiol 4-88 (Calbiochem ...
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bioRxiv - Microbiology 2023Quote: ... DNA was de-crosslinked by proteinase K (Sigma, USA) and 4 M LiCl and purified by phenol ...
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bioRxiv - Microbiology 2023Quote: ... the DNA was transferred onto the Immobilon membrane (Millipore) by vacuum-based blotting ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was fragmented by adding 1:100 Benzonase (Sigma). Lysates were heated to 70°C in SDS sample buffer with 50 mM DTT for 10 minutes ...
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bioRxiv - Pathology 2023Quote: ... Genomic DNA was removed by DNase I (Sigma-Aldrich) treatment following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2023Quote: ... Nuclei were visualized with the DNA stain (DAPI; Sigma) added at 5 µg/mL with the secondary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μM purified sscGAS was incubated testis DNA (Sigma) for 24 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was dissolved in 1X TE (Sigma-Aldrich) (10 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... or ROCHE Taq DNA polymerase (Sigma-Aldrich, cat# 11596594001). The RT-PCR products were then resolved by electrophoresis in 2% agarose gels alongside GeneRuler 1 kb Plus DNA Ladder ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid DNA constructs were cloned into pET-24b (Novagen) expression vector by site-directed mutagenesis and the coding sequences of all mutants were confirmed by DNA sequencing prior to use.
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bioRxiv - Cancer Biology 2023Quote: ... DNA constructs were transfected using PEI (polyethylenimine, Sigma Aldrich) in HEK293 ...
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bioRxiv - Biochemistry 2023Quote: ... DNA- and RNA-free BSA was used (Sigma Merck) and diluted to the final concentration in the sample buffer as described above for aS ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was purified using chloroform isoamyl alcohol (Sigma Aldrich) and eluted with 25 µl ddH2O ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was then purified with phenol/chloroform (Sigma-Aldrich) followed by ethanol/sodium acetate precipitation ...
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bioRxiv - Neuroscience 2023Quote: ... DNA was extracted by phenol/chloroform (Sigma-Aldrich 77617) followed by ethanol precipitation ...
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bioRxiv - Genetics 2023Quote: ... and X-tremeGENE™ HP DNA Transfection Reagent (Sigma), following the protocol described by Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was purified using chloroform isoamyl alcohol (Sigma Aldrich) and eluted with 25 μl ddH2O ...
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bioRxiv - Microbiology 2023Quote: ... DNA was visualized using the Immobilon Western kit (Millipore) and photographed by a Tanon 5200 Multi-Imaging System (Tanon ...
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bioRxiv - Developmental Biology 2024Quote: ... and DNA stain (Hoechst34580 1:1000 Sigma Aldrich 63493). Samples were then washed with PTw 3 times and mounted into 85% glycerol or Vectashield Plus for imaging.
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bioRxiv - Cancer Biology 2023Quote: ... and separated from DNA by using chloroform (Sigma-Aldrich). Isopropanol (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2024Quote: ... genomic DNA was extracted using Cetyltrimethylammonium-bromide (CTAB) (Sigma), and TAIL PCRs were conducted using insertion specific primers (RB1 and RB2 ...
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bioRxiv - Microbiology 2023Quote: ... using a SeqPlex DNA Amplification Kit (Sigma-Aldrich, USA), which is specifically designed for low-quantity ...
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bioRxiv - Cancer Biology 2023Quote: ... and JumpStart™ Taq DNA Polymerase (D9307, Sigma-Aldrich) to ensure cells were free of mycoplasma contamination.
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bioRxiv - Plant Biology 2024Quote: ... were amplified with KOD Hot Start DNA Polymerase (Novagen) and cloned into pDONR/Zeo vector (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... and 15 mg of the tissue from each mouse liver was used for genomic DNA extraction using GenElute Mammalian Genomic DNA Miniprep Kit (Millipore Sigma Cat. #: G1N350).
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bioRxiv - Microbiology 2019Quote: ... DNA was prepared from a 3 ml culture of the representative strain using the GenElute ™ Bacterial Genomic DNA Kit (Sigma Aldrich, Sweden) [7].
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bioRxiv - Immunology 2021Quote: ... The pyrosequencing assay was validated using SssI-treated human genomic DNA as a 100% methylation control and human genomic DNA amplified by GenomePlex Complete Whole Genome Amplification kit (Sigma-Aldrich, WGA2-50RXN) as 0% methylation control ...
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bioRxiv - Genetics 2024Quote: ... The sonicated DNA was then precleared with 50 µl of Protein A agarose beads/Salmon sperm DNA slurry (EMD Millipore, Catalog # 16–157) for one hour on a rotator in cold ...
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bioRxiv - Biochemistry 2022Quote: ... we first extracted the genomic DNA (gDNA) from each cellular fraction using the GenElute Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich, St. Louis, MO). A previously described semi-nested polymerase chain reaction (PCR ...
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bioRxiv - Biochemistry 2021Quote: ... Each cellular fraction was then expanded and harvested prior to the extraction of genomic DNA using the GenElute Mammalian Genomic DNA Miniprep kit (Sigma-Aldrich, St. Louis, MO). A semi-nested PCR approach was then used to selectively amplify the mutagenized region of the polyprotein reporter from the genomic integration site in a similar manner as was previously described (Matreyek et al. ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNAs were prepared from selected isolates showing different cell and colony morphologies using GenElute™ Bacterial Genomic DNA Kit (Sigma Aldridge, Gillingham, UK). Identification to presumptive species level was carried out by performing PCR amplification of 16S rRNA gene sequences using primers TPU1 and RTU8 (73 ...
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bioRxiv - Genomics 2021Quote: ... genotyping for the Ile148Met variant associated with hepatic steatosis (rs738409) was performed on genomic DNA extracted from the blood buffy coat using the GenElute Blood Genomic DNA kit (Sigma, St. Louis, MO, USA). rs738409 was genotyped using validated primers and TaqMan probes (Applied Biosystems ...
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bioRxiv - Genomics 2019Quote: ... Half of the sample was proceeded for genomic DNA isolation using GenElute plant genomic DNA miniprep kit (Sigma-Aldrich Co., St. Louis, MO, USA) according to the manufacturer’s instructions for PacBio and Illumina platforms sequencing ...
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bioRxiv - Biophysics 2021Quote: ... Unpackaged DNA was degraded by adding DNase I (Sigma-Aldrich) by incubating this mixture for 30 min at 37°C ...
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bioRxiv - Genetics 2021Quote: ... DNA was stained with 0.5 mg/ml DAPI (Sigma-Aldrich). Images were captured using a Zeiss Axioplan 2 fluorescence microscope and edited with Adobe Photoshop.
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bioRxiv - Microbiology 2022Quote: ... aeruginosa stained with the DNA dye thiazole orange (Sigma Aldrich).
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bioRxiv - Biophysics 2020Quote: ... PCR was performed with KOD Hot Start DNA polymerase (Novagen) and oligonucleotides were synthesized by Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... 5% sheared salmon sperm DNA (Sigma, St. Louis, MO, USA), 0.3% yeast tRNA (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... Genomic DNA was extracted from tails by Proteinase K (Sigma) digestion and genotyping performed by polymerase chain reaction (PCR ...
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bioRxiv - Genomics 2019Quote: ... with 0.25 units of JumpStart Taq DNA Polymerase (Sigma Aldrich) in a total volume of 25 µl ...
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PHF2 regulates homology-directed DNA repair by controlling the resection of DNA double strand breaksbioRxiv - Molecular Biology 2019Quote: Plasmid DNA was transfected into cells using Polyethylenimine (Sigma Aldrich) or Lipofectamine 2000 (Invitrogen).
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bioRxiv - Plant Biology 2019Quote: ... The membranes were probed with anti-GAL4 DNA-BD (Sigma) for the HMA domains in pGBKT7 and anti-GAL4 activation domain (Sigma ...
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bioRxiv - Microbiology 2019Quote: Agents that promote DNA damage were obtained from Sigma-Aldrich (methyl methanesulfonate ...
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bioRxiv - Developmental Biology 2019Quote: ... DNA was labeled using Hoechst (Hoechst 33342, Sigma, 1:1000) and actin was detected using Alexa647-phalloidin or Alexa555-phalloidin (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The DNA pellet was washed in 70% ethanol (Sigma; E7023), resuspended in sterile MilliQ water and used as template DNA for polymerase chain reaction (PCR) ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragments encoding shRNAs directed against human CDH13 mRNA (Sigma) were cloned into the pTRIPΔU3-EF1α-EGFP lentiviral vector ...
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bioRxiv - Cell Biology 2020Quote: ... DNA was stained with DAPI-containing Fluorosheild mounting medium (Sigma). Images were acquired on a Leica SP8 confocal microscope usually using 100 × objective (NA=1.40 ...
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bioRxiv - Plant Biology 2021Quote: ... DNA was recovered by Phenol:Chloroform:Isoamilic Acid (25:24:1; Sigma) extraction and ethanol precipitation ...