Labshake search
Citations for Millipore Sigma :
551 - 600 of 764 citations for Cy7 Dyes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... 5 μL of the dye solution was added to 1 mL 5 mg/mL HI (91077C, Sigma-Aldrich, 95%) monomer solution ...
-
bioRxiv - Microbiology 2022Quote: ... DNA was stained with Hoechst by incubating cell on glass coverslips with 2 mg/ml bisbenzimide (Hoechst dye. Sigma) for 30 min ...
-
bioRxiv - Microbiology 2020Quote: ... Cultures were diluted to a final concentration of 1 × 106 CFU/mL in sterile 1X PBS and were stained in the dark for membrane potentials using 30 μM membrane potential indicator dye DIO2(3) (3,3=-diethyloxacarbocyanine iodide) (320684; Sigma) for 30 min ...
-
bioRxiv - Neuroscience 2020Quote: ... We verified primary motor neuron identity in a small subset of recordings from eYFP-expressing cells in Tg(mnx1:GAL4;UAS:ChR2(H134R)-eYFP) larvae by adding 0.025% sulforhodamine-B acid chloride dye in the intracellular solution (Sigma-Aldrich) and filling the neuron to reveal its morphology ...
-
bioRxiv - Microbiology 2021Quote: ... lysed in Laemmli SDS-loading dye and separated in 15% acrylamide gels and transferred to 0.45 μm hydrophobic Immobilon-P PVDF membrane (Merck Millipore). Immunoblotting was as described previously 19 ...
-
bioRxiv - Developmental Biology 2019Quote: ... flies were placed in a vial with blue-dyed yeast paste (10% FCF Brilliant Blue Dye (Sigma-Aldrich, #80717) in water mixed with dry yeast ...
-
bioRxiv - Cancer Biology 2019Quote: ... Eighteen microliters of high-titer lentivirus expressing miR-382 mixed with 2 μL 0.2% Evans blue dye (Sigma-Aldrich) in PBS was injected in the third mammary gland using a 34-gauge needle (Hamilton) ...
-
bioRxiv - Neuroscience 2020Quote: ... Electrodes were coated with 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl-indocarbocyanine perchlorate fluorescent dye (DiI, Sigma; (DiCarlo et al., 1996)) ...
-
bioRxiv - Biophysics 2019Quote: ... the dye coated coverslip sample was prepared by first adding 200 µL poly-L-lysine (P4707-50ML, Sigma-Aldrich) onto a 25 mm round coverslip and was incubated for 1 hour ...
-
bioRxiv - Neuroscience 2019Quote: ... For this study we injected 150 μl (2% V/V% in saline) of Evans blue dye (Sigma-Aldrich, E2129) intra-peritoneally into three C57BL/6J and CD1-Elite male ...
-
bioRxiv - Physiology 2020Quote: ... with the following modifications: we deposited a mix of 50% yeast/0.75% of the food blue dye Erioglaucin (ref 861146, Sigma) in the center of a Petri dish lid (diameter ...
-
bioRxiv - Cell Biology 2021Quote: ... Supernatants were collected and mixed with loading buffer (60 mM Tris pH 6.8, 10% glycerol, Orange G dye (Sigma), and 100 mM fresh DTT) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were cultured for 72 h and then cell death was measured using the tetrazolium dye (2,3)-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-terazolium-5-carboxanilide (XTT) assay (Sigma) according to manufacturer’s instruction ...
-
bioRxiv - Microbiology 2020Quote: ... intracellular ROS production is detected by staining cells with the ROS-sensitive fluorescent dye DCFH-DA (2,7-dichlorofluorescein diacetate; Sigma). Cells from 25 ml cultures grown at 30 °C overnight in YPD medium are collected and washed twice with PBS ...
-
bioRxiv - Immunology 2020Quote: ... Production of reactive oxygen species (ROS) in kidney neutrophils was evaluated ex vivo with 1,2,3 dyhidroxyrhodamine (DHR) dye (Sigma Aldrich). Single cells isolated from kidneys of mice not exposed to UV light (D-1 ...
-
bioRxiv - Immunology 2020Quote: The assay for membrane depolarization activity of rBjRPS15 and BjRPS1545-67 was performed with the membrane potential-sensitive dye 3,3’-dipropylthiacarbocyanine iodide (DiSC3-5; Sigma) and the bacteria hydrophila ...
-
bioRxiv - Neuroscience 2020Quote: ... with 1:50 dilution of the dye in water from Milli-Q water purification system (Millipore, Bedford, MA, USA).
-
bioRxiv - Neuroscience 2021Quote: ... the animals were infused with 2 ml/kg of 2% Evans blue dye in saline (Sigma Aldrich, MO, USA) through the tail vein ...
-
bioRxiv - Biochemistry 2022Quote: ... protein destined for dye labeling was concentrated in an Amicon Ultra-15 centrifugal filter units (30 kDA, Millipore #UFC9030). These samples were then mixed at a molar ratio of 1:1 with Cyanine3 NHS-Ester (Lumiprobe #21020 ...
-
bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 S expression HEK293T cells were stained with PKH26 dye according to the manufactures protocol (Sigma-Aldrich). Next ...
-
bioRxiv - Molecular Biology 2023Quote: Drug efflux by MDR1 was measured with the Multidrug Resistance Direct Dye Efflux Assay kit (Merck Millipore; ECM 910) according to the manufacturer’s protocol ...
-
bioRxiv - Bioengineering 2023Quote: ... The electroporated mixture was transferred into a 1.5 mL tube to which is added PKH26 dye (0.4 µL) (PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, Sigma) mixed with 100 µL diluent C ...
-
bioRxiv - Neuroscience 2023Quote: ... tetrode wires and the tips of the silicon probes were coated with a lipophilic fluorescent dye (DiI; Sigma-Aldrich), allowing us to visualize tetrode and probe locations at the end of each experiment ...
-
Plasma Activated Water as a Pre-Treatment Strategy in the Context of Biofilm-Infected Chronic WoundsbioRxiv - Microbiology 2023Quote: PAW-induced outer membrane permeability was measured based on fluorescent dye N-phenyl-1-naphthylamine (NPN; Sigma-Aldrich, Australia) uptake [22] ...
-
bioRxiv - Biophysics 2023Quote: ... Unbound dye was removed by sequential buffer exchange in centrifugal filters Amicon Ultra 0.5 ml 30 K (Merk Millipore). Finally ...
-
bioRxiv - Biophysics 2023Quote: ... Unbound dye was removed by sequential buffer exchange in centrifugal filters Amicon Ultra 0.5 ml 3 K (Merk Millipore). Lge11-80-Dylight labelled protein was stored at -80 °C.
-
bioRxiv - Cancer Biology 2023Quote: ... Dye transfer from DiI-stained ALL cells cultured in transwells towards MSCs cultured in 6-well plates (Sigma Aldrich) was determined after 40 hours by flow cytometry ...
-
bioRxiv - Biophysics 2023Quote: His-FUS LC was labeled with Atto-488 or Atto-647 NHS-ester fluorescent dyes (ATTO-TEC, Sigma-Alrich). Labeling occurred at or near the N-terminus because only the N-terminus and a lysine at residue position 5 in the leader sequence preceding the N-terminal region hexa-histidine tag were expected to react with the NHS-functionalized dye ...
-
bioRxiv - Microbiology 2023Quote: ... 10-fold serial dilutions from 1 x 106 to 1 x 103 cells/mL were incubated with 0.5 mg/mL MTT dye (Sigma) for 2 h ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The quality of protein transfer was visualized by staining with Ponceau S (0.1% dye in 5% acetic acid, Sigma) and then blocked with 5% nonfat instant milk (Carnation ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... 75 μl PKH26 dye (0.5 μM, PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling, Sigma-Aldrich, Germany) was applied at least 5 days before imaging via oropharyngeal application ...
-
bioRxiv - Physiology 2024Quote: Cells were stained with the DNA-binding dyes propidium iodide (PI) and Hoechst 33342 (10 µg/mL, Sigma-Aldrich) to count apoptotic cells by fluorescent microscopy ...
-
bioRxiv - Cell Biology 2019Quote: Bulk endocytosis and delivery of extracellular materials to the late endosomal compartment was measured using the dye Sulforhodamine 101 (SR101, Sigma), following published protocols (42 ...
-
bioRxiv - Cell Biology 2020Quote: ... the samples were incubated with secondary antibody conjugated with 2 µg/ml Alexa dye (Thermo) and 300 nM DAPI (Sigma) in blocking solution for 1 hour at room temperature ...
-
bioRxiv - Microbiology 2020Quote: This drug susceptibility assay was performed essentially as described [63] and is based on only live cells reducing the blue and non-fluorescent viability indicator dye resazurin sodium salt (‘Alamar blue’; Sigma) to pink ...
-
bioRxiv - Biochemistry 2019Quote: ... and absorbance scans were performed on a spectrophotometer (Shimadzu) to identify a region of spectral overlap in absorbance maxima between the proteins and Rose Bengal dye (# 330000, Sigma). The most robust overlap occurred between 550-580 nm (Fig ...
-
bioRxiv - Cell Biology 2019Quote: ... Covalently modified αSYN was separated from non-reacted dye on a Sephadex G-25 column (Amersham, GE) and concentrated with 6 kDa MW cut-off spin columns (Millipore). The final concentration of the fluorescently labeled protein was measured by UV-VIS spectrophotometry at 280 nm with E ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells that had invaded the underside of the inserts were fixed with 100% methanol for 10 minutes at −20°C and stained with 0.5% crystal violet dye (EMD Millipore) for 10 min at room temperature ...
-
bioRxiv - Neuroscience 2019Quote: ... The DNA (3–4 μg/μl) together with the dye Fast Green (0.3 mg/ml; Sigma, St Louis, MO, USA) was injected (~1 μl ...
-
bioRxiv - Bioengineering 2020Quote: ... cells on different GO and RGO substrates were collected and incubated with BioTracker™ CyP AP dye (10 μM) (Millipore) in MEM medium for 30 min at 37 °C followed by PBS washing.47 Cellular ALP activity catalyzes cleavage of the phosphate group in CyP ...
-
bioRxiv - Biochemistry 2020Quote: A protein/Sypro orange mix containing 5 μM protein and a 1:5,000 dilution of dye in DMSO (as supplied by Sigma Aldrich) was prepared just before plate setup in 20 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2020Quote: The Congo red binding (CRB) ability of the APEC isolates was determined using agar plates supplemented with 0.003% CR dye (Sigma, USA) and 0.15% bile salts ...
-
bioRxiv - Microbiology 2020Quote: ... OD600nm adjusted to 0.1 (measured in 100 µL volume in a clear flat bottom 96 well plate) and supplemented with 1.6 mg/ml Red#40 dye (Sigma, Israel). Fifty µL of this culture were pipetted to rows A-G of the plate containing the amoeba ...
-
bioRxiv - Molecular Biology 2020Quote: ... All samples were then boiled at 95 °C for 10 mins at a 1:1 (v/v) ratio in 2x Laemmli loading dye (Sigma) before being electrophoresed through a 4-12% NuPAGE Bis-Tris polyacrylamide gel (BioRad) ...
-
bioRxiv - Cell Biology 2021Quote: The glycogen concentration was measured by a colorimetric assay based on the peroxidase sensitive dye 3,3′,5,5′-tetramethylbenzidine (TMB) (Sigma-Aldrich #860336). Glycogen was digested to glucose using amyloglucosidase (AG) ...
-
bioRxiv - Cell Biology 2021Quote: ... The protein solution was then filtered through a 0.22 μm filter and free dye removed by buffer exchange into gel filtration buffer in Amicon ultra 10 kDa spin concentrator (Millipore). The extent of labeling was estimated based on absorbance at 280 and 647 nm.
-
bioRxiv - Molecular Biology 2022Quote: ... On the 6th day post-induction of differentiation the cells were fixed with 3.7% formaldehyde solution followed by staining with Oil Red-O dye (Sigma-Aldrich) (prepared in 60% isopropanol ...
-
bioRxiv - Immunology 2022Quote: ... the different mouse groups were killed 1 hour after retro-orbital injection of 100 μl of 2% Evans blue (EB) dye (Sigma) and were perfused intracardially with 15 ml of PBS ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were washed thrice with 0.1% Triton-X in PBS followed by incubation with secondary antibodies and DAPI (nuclear dye) (Sigma, USA) for 2 hr at room temperature ...
-
bioRxiv - Neuroscience 2020Quote: Apoptotic cells were revealed in 2-days-old live embryos by adding the Acridine Orange vital dye (N-4638; Sigma) to the embryo water at a final concentration of 5 mg/ml ...