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Citations for Millipore Sigma :
201 - 250 of 7211 citations for Crimean Congo Hemorrhagic Fever Virus Gn Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and resuspending in virus + polybrene (final conc 8 ug/mL, Sigma-Aldrich, TR-1003) or RPMI media + polybrene for the uninfected control at a concentration of 1E6 cells/mL ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed and virus was concentrated using an Optiprep density gradient (Sigma #D1556). Small-scale AAV virus was produced in HEK293T cells grown in 12-well plates by triple-transfection with 0.4μg HiUGE vector ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus (400 µL) was added together with 5 ug/mL of polybrene (Sigma-Aldrich) and cells were centrifuged at 2,000 rpm for 2 h at 30°C ...
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bioRxiv - Cell Biology 2024Quote: ... The virus was resuspended by fresh MEFs medium with 8 μg/ml polybrene (Sigma).
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bioRxiv - Cell Biology 2024Quote: ... Cell lines were transduced with a mix of supernatant containing virus and polybrene (Sigma H9268 suspended at 4mg/ml in sterile water ...
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bioRxiv - Microbiology 2021Quote: ... The filtered virus was mixed with fresh media (30% vol/vol) that included polybrene (Sigma) at a 6 μg/ml final concentration ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 µL of virus was added per well with 10 µg/mL of polybrene (Sigma) in 100 µL of EMEM without antibiotics or serum overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Primer extension was conducted with 4 units of Avian Myeloblastosis Virus (AMV) reverse transcriptase (Sigma) for 1 hour at 15°C ...
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bioRxiv - Cancer Biology 2019Quote: ... concentrated virus was added to the cells along with 8 ng/mL polybrene (Sigma-Aldrich) and the cells were incubated at 37°C plus 5% CO2 in humidified air ...
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bioRxiv - Cancer Biology 2021Quote: ... virus was washed out and replaced with fresh complete medium with 10μg/ml blasticidin (Sigma). After 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5 mL of freshly thawed virus soup was mixed with polybrene (Cat# H9268, Sigma-Aldrich) to a final concentration of 8 μg/mL and used to infect A549N and HT-1080N cell lines ...
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bioRxiv - Microbiology 2021Quote: ... Virus lytic replication was induced via addition of 2 µg/mL doxycycline hyclate (Sigma-Aldrich). All cells were cultured at 37 °C at 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: Virus titres were quantified by plaque assay in 12-well plates (Millipore Sigma, Cat# Z707775). For the quantification of FCV ...
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bioRxiv - Neuroscience 2019Quote: ... The virus was subsequently concentrated by mixing the filtered supernatant with 50% PEG-8000 (Sigma) in a ratio of 4:1 and placed at 4°C over night ...
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bioRxiv - Immunology 2020Quote: ... sucrose gradient-purified AD169r-GFP virus was transferred to a 100,000 kDa Amicon filter (Millipore), then buffer exchanged with 1× PBS ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... The virus particles were then concentrated to 300µl using Amicon Ultra Centrifugal Filters (UFC910024 SIGMA) by spinning at 1000g at 4°C for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... harvested virus was added onto target cells in the presence of 8μg/ml polybrene (Millipore). For in vivo experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were infected with virus-containing media using Polybrene reagent (TR-1003-G; EMD Millipore) according to the manufacturer’s instructions and selected for 48 hr in 2 μg/mL of puromycin.
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bioRxiv - Immunology 2020Quote: ... We generated HAdV-lactoferrin complexes by incubating the virus with 40 μg lactoferrin (Sigma-Aldrich) for 30 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Unabsorbed virus was removed and 2 ml of culture medium supplemented with 1% methylcellulose (Sigma) were added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... Virus-containing supernatant was concentrated using Amicon Ultra-15 100kDa MWCO centrifugal filters (Millipore, UFC910024). For siRNA knockdown experiments ...
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bioRxiv - Cell Biology 2022Quote: ... Undiluted virus was added dropwise to cells with 10 μg/mL polybrene (Sigma-Aldrich; TR1003G), and incubated at 37°C for 24 hrs ...
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bioRxiv - Microbiology 2023Quote: ... the virus inoculum was removed and phenol-red free DMEM supplemented with .3% BSA (Sigma), 100U/ml pen/strep (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing cell supernatants were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore) and exchanged into TexMACS media (Miltenyi Biotec).
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bioRxiv - Microbiology 2023Quote: ... the virus inoculum was removed and phenol-red free MEM supplemented with 3% BSA (Sigma), 100U/mL of penicillin and 100ug/mL of streptomycin mixture (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... virus stocks were concentrated on a 100 KDa cellulose membrane (Amicon Ultra-15, Merck Millipore) and rinsed three times successively with PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... virus at the MOI of 50 was applied with 8 μg/ml Polybrene (Merck Millipore). Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... the virus-containing supernatant was filtered through a 0.45-μm pore size PVDF filter (Millipore), and lentiviral particles were concentrated using the Lenti-X Concentrator (Takara ...
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bioRxiv - Cell Biology 2020Quote: ... Virus stock was filtered by centrifugation using Amicon Ultra-15 Centrifugal filter unit (Sigma, Cat # UFC910096) and resuspended in viral propagation media ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting supernatant or ‘secondary virus’ was filtered using 0.45 μm pore size sterile filter (Millipore) and was used immediately to infect large-scale expression cultures ...
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bioRxiv - Cell Biology 2022Quote: ... the medium containing the virus particles was recovered and concentrated with Polyethylene Glycol 8000 (Sigma-Aldrich) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... cells were infected with serial dilutions of virus diluted in 1× minimum essential medium (MEM, Sigma) supplemented with 0.6% BSA (Sigma) ...
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bioRxiv - Genomics 2020Quote: ... HeLa cells were incubated overnight in growing media plus virus and polybrene (Sigma-Aldrich, TR-1003). The following day ...
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bioRxiv - Molecular Biology 2019Quote: ... Supernatant was collected after 48hrs and 72hrs and virus was concentrated using Polyethylglycol (PEG, Sigma # P4338). V6.5 cells were infected in medium containing 5ug/ml polybrene (Millipore ...
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bioRxiv - Microbiology 2021Quote: Virus propagation was performed in Vero E6 cells in DMEM (Sigma-Aldrich, St Louis, MO, USA) supplemented with 2% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transduced with the respective concentrated virus particles using 10 µg/mL polybrene (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... FFUs were visualized using MAB8705 Anti-dengue Virus Complex Antibody clone D3-2H2-9-21 (Millipore) and goat anti-mouse Alexa Fluor 488 (A-11001 ...
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bioRxiv - Cell Biology 2019Quote: ... the virus containing suspension from HEK293T cells was filtered with a 0.45 μM filter (EMD Millipore), supplemented with polybrene (4 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... Virus was added at the indicated titer in the presence of polybrene (2µg/mL, Sigma, H9268) and the cells were incubated for 24 h ...
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bioRxiv - Immunology 2021Quote: ... the antibody-virus mixture was removed and 100µl of prewarmed 0.85% methylcellulose (Sigma-Aldrich, #M0512-250G) overlay was added to each well ...
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bioRxiv - Microbiology 2021Quote: ... All virus propagation was performed in VeroE6 cells cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... The virus containing supernatant was mixed with polyethylene glycol 6000 (Sigma Aldrich, Saint Louis, Missouri, US) (8 % wt/v ...
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bioRxiv - Systems Biology 2022Quote: ... virus particles were precipitated by adding 50% (wt/vol) polyethylene glycol 6000 (PEG 6000) (Sigma-Aldrich) in NT buffer (150 mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: ... culture supernatants containing virus were harvested and passed through a 0.45 μM PVDF filter (Millipore, SLHV033RS). Viral titers were calculated and an MOI of 1 was used for transduction ...
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bioRxiv - Microbiology 2024Quote: ... the virus/glycan-mixture is incubated with 0.5 U heparinase I and III (H3917, Sigma-Aldrich) for 1 h at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... and concentrated using fast-trap virus purification and concentration kit according to manufacturer′s instructions (Millipore). For transduction ...
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bioRxiv - Cell Biology 2020Quote: ... Virus was collected 24- and 48-hours post-transfection and filtered through a 0.45µm filter (EMD Millipore), and used for transduction of 4T07 ...
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bioRxiv - Molecular Biology 2021Quote: ... After 2h of virus adsorption in the presence of 0.2 mg/ml of porcine pancreatin (Sigma-Aldrich) prepared in CMGF(-) ...
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bioRxiv - Cell Biology 2019Quote: ... the virus-containing medium was removed and replaced with selection medium containing 2µg/ml Puromycin (Sigma-Aldrich). After 7 days of selection ...