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Citations for Millipore Sigma :
201 - 250 of 6912 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Cells obtained from overnight cultures grown in YPD broth at 37°C were spotted in serial 10-fold dilutions on YP plates containing 2% glycerol or YPD plates containing 0.05 mg/ml of Congo red (Millipore Sigma) or 0.01 mg/ml of calcofluor white (Millipore Sigma) ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 µL of stationary phase cultures of the complemented strains were spotted in BHI agar plates supplemented with Congo Red (Sigma) 25 µg.mL-1 and IPTG 1 mM ...
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bioRxiv - Microbiology 2023Quote: ... The nylon filter resuspended in 1 mL TSB and plated out onto TSA containing 0.01% Congo red dye (Sigma-Aldrich), Cb60 ...
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bioRxiv - Microbiology 2023Quote: ... 40 μl of overnight LB broth cultures of STm wild type strain 14028s and STy wild type strain H58 were spotted on agar plates containing 1% w/v Tryptone and 0.5% w/v Yeast Extract supplemented with Congo Red (40 μg/ml) (Millipore Sigma) and kept at 30°C for two days.
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bioRxiv - Microbiology 2023Quote: ... were tested by culturing the necropsy samples on Columbia sheep blood agars (Biolab Inc., Hungary) and sheep blood agars supplemented with nicotinamide adenine dinucleotide (Sigma-Aldrich Co., USA) at the final concentration of 20 µg/ml ...
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bioRxiv - Pathology 2022Quote: ... the virus particle-containing media was harvested and virus particles were purified using Polyethylene glycol 8000 (Sigma).
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bioRxiv - Microbiology 2019Quote: ... DNA encoding the POTRA domains (1-4) of BamA with a C-terminal strep-tag (but without the signal sequence) was cloned in pET21a (Novagen). All the proteins above were overexpressed in BL21(DE3 ...
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bioRxiv - Microbiology 2021Quote: ... encoding PPK with a C-terminal GAAEPEA peptide tag for affinity purification (105) between the NdeI and HindIII sites of plasmid pET-21b(+)(Novagen), was synthesized by GenScript.
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bioRxiv - Molecular Biology 2019Quote: ... Mouse CTCF cDNA corresponding to the full-length 736 amino acids was cloned with C-terminal 6xHis tag into pFLAG-2 (Sigma). FLAG-CTCF-6xHis protein was induced in Rosseta-Gami B-cells (EMD Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... The activated beads were resuspended in 100 μL 50 mM MES (pH 5.0) and coupled with 10 μg carrier-free anti-epitope tag antibody (c-myc: Santa Cruz #sc-40; FLAG: Sigma #F1804 ...
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bioRxiv - Biophysics 2019Quote: ... Tag cleavage was carried out overnight at 4 °C on a roller with 10 units of HRV 3C protease (Novagen/Merck chemicals Boulevard Industrial Park ...
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bioRxiv - Evolutionary Biology 2020Quote: ... brucei ALPH1 was expressed as an N-terminal truncation with a C-terminal 6xHis-Tag in RosettaBlue competent bacteria cells (Novagen) as previously described [10].
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bioRxiv - Microbiology 2022Quote: ... and incubated overnight at 4°C with specific primary monoclonal (MAbs) or polyclonal (pAbs) antibodies: anti-HA tag (rabbit pAb; H6908, Sigma), anti-GFP (rabbit pAb ...
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bioRxiv - Cell Biology 2019Quote: ... carrying two isoleucine residues at the N-terminus and a hexahistidine tag at the C-terminus was expressed in Escherichia coli Rosetta2(DE3)pLysS cells (Novagen) and purified by immobilized metal-affinity chromatography followed by gel-filtration chromatography as described previously (Bishop et al. ...
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bioRxiv - Microbiology 2019Quote: ... This fragment was cloned by ligation independent cloning into the linearized pIEX-10 EK-LIC vector which carries a C-terminal 10xHis tag (Novagen) to generate pIEX-10 ...
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bioRxiv - Microbiology 2019Quote: ... was cloned into the protein expression vector pET28a (+) containing an IPTG-inducible promoter and a C-terminal 6x-His tag (Novagen). Expression of CosR-His was then induced in E ...
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bioRxiv - Genetics 2023Quote: ... 0.1% Tween-20 and 5% non-fat milk for 1 hour at room temperature and probed for 18 hours at 4 °C with antibodies specific for Flag tag (M2, SIGMA), Myc tag (9E10 ...
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bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
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bioRxiv - Biochemistry 2023Quote: ... the Streptag-purified protein after removal of the C-terminal affinity-GFP-tag was collected and concentrated using 100 kDa MW cut-off spin concentrator (Amicon Merck-Millipore) further purified by size-exclusion chromatography (SEC) ...
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bioRxiv - Biochemistry 2023Quote: ... or a VSVG epitope tag on the N-terminal tag of H2A (Sigma, V4888) and a fluorescently labelled secondary antibody (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... were spin transduced with 10-20μl of the concentrated virus at 37°C at 2200g for 90 minutes with polybrene reagent (8-10μg/ml, Millipore Sigma, Billerica, MA). The cells were washed with PBS and resuspended in fresh RPMI media supplemented with 10% FBS with 1% penicillin and streptomycin 24 hours post-transduction ...
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bioRxiv - Cell Biology 2019Quote: ... containing 10% FCS (Sigma), 100U/ml penicillin and 100µg/ml streptomycin (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... 10% FCS (Sigma-Aldrich), 1% Penicillin-Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... supplemented 10% FCS (Sigma), 1% Pen Strep (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... 10% FCS (Sigma Aldrich), 100 units/ml penicillin ...
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bioRxiv - Immunology 2022Quote: ... 10% FCS (Sigma-Aldrich) and 50 U/ml Pen/Strep (Gibco ...
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bioRxiv - Pathology 2019Quote: ... FCS (10 %) (Sigma-Aldrich), b-FGF (10 ng/ml) ...
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bioRxiv - Immunology 2019Quote: ... and 2% FCS (Sigma) adjusted to pH 7.4 at 37°C and sterile filtered) ...
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bioRxiv - Microbiology 2020Quote: ... 2% FCS (Sigma-Aldrich), 20 mM HEPES buffer (Gibco) ...
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bioRxiv - Immunology 2020Quote: ... 5% FCS (Sigma-Aldrich), 20 μg/ml gentamycin (Sigma-Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... 12.5% FCS (Sigma-Aldrich), 1% PenStrep and 100IU/ml interleukin-2 (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... Fc control (Millipore, #401104), ephrinB1-Fc (R&D ...
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bioRxiv - Cancer Biology 2021Quote: ... Flag-tag (Sigma, F3165-.2MG), Veriblot IgG (Abcam ...
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bioRxiv - Genomics 2019Quote: ... Flag-tag M2 (F1804, Sigma), goat IgG control (sc-2028 ...
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bioRxiv - Microbiology 2019Quote: ... Flag-tag (Sigma-Aldrich, 1;1000) and β-actin (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... Anti-FLAG tag (Sigma, #F1365), anti-PGK1 (Thermo # PA5-28612) ...
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bioRxiv - Molecular Biology 2020Quote: ... HIS-tag (Sigma-Aldrich H1029), and Streptactin-HRP (ThermoFisher Scientific #21130) ...
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bioRxiv - Cell Biology 2021Quote: ... S tag (EMD Millipore; MAC112), IRDye 680RD Streptavidin (LI-COR ...
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bioRxiv - Cell Biology 2022Quote: ... anti-S-tag from Novagen, anti-V5 from GenScript and anti-GFP from Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... HA-Tag (Sigma H3663-200UL), ACF1 (Abcam ab187670) ...
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bioRxiv - Microbiology 2023Quote: The His-tag system (Novagen) was used for Sif purification ...
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bioRxiv - Biochemistry 2023Quote: ... and FLAG tag (Sigma, F3165).
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bioRxiv - Biophysics 2021Quote: A 3 μL droplet of the 500 μM peptide solution was applied to glass slides and stained with a 10 mM Congo Red (Sigma©) solution in PBS (137 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Biofilm matrix composition was investigated through spotting 10 μL of each mutant (representing 105 CFU) on agar supplemented with 40 μg/mL Congo red (Sigma, C6277) to examine curli production ...
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bioRxiv - Molecular Biology 2022Quote: ... until 10-7 and 10 µl of the dilutions between 10-5 and 10-7 were plated in BHI agar plates supplemented with Congo Red (Sigma®) at a final concentration of 25 µg.ml-1 ...
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bioRxiv - Microbiology 2020Quote: ... 500 μl of supernatant from cultures incubated with 50 μM or 150 μM of the compounds for 4 hours or 8 hours was mixed with 1 ml of 30 mM Tris-HCl buffer containing 10 mg/ml of Elastin-Congo Red (Sigma-Aldrich). The mixture was then incubated with shaking at 37°C for 1–2 hours ...
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bioRxiv - Plant Biology 2023Quote: Glucanase activity can be detected by yellow halos against the red background after staining with 0.1% Congo Red (Sigma-Aldrich, C6767) dye solution (solved in 50% ethanol ...
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bioRxiv - Developmental Biology 2021Quote: ... then blocked in PBT plus 5% normal sheep serum (Sigma) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... was diluted 1:400 in 2.5% sheep serum (Sigma-Aldrich). Incubations were done for at least 36h at 4° C shaking and after each incubation time ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were subsequently blocked using 5% sheep serum (Sigma-Aldrich) (r.t. ...