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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Cow Claudin 1 CLDN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Brain cell-specific origin of circulating microRNA biomarkers in experimental temporal lobe epilepsybioRxiv - Neuroscience 2023Quote: ... Lysates were removed and protein antibody-conjugates linked to the bottom of the plate were washed three times with 300 µL of ELISA wash buffer (100mM Tris buffered saline, pH8.2, 2mM MgCl2, 0.5% Tween-20 – all Sigma-Aldrich, Ireland). Protein was eluted by incubating wells in 2 X Laemelli buffer for 10 mins ...
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bioRxiv - Immunology 2022Quote: ... antibody levels were detected using a secondary anti-murine IgG conjugated with alkaline phosphatase and the substrate for alkaline phosphatase yellow (pNPP) liquid substrate system for ELISA (cat. P7998, Sigma). After 30 minutes of incubation ...
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bioRxiv - Cell Biology 2021Quote: VCAM-1 in the media (1:100) from MCECs stimulated with MG was measured using commercial kits from Sigma, according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: RNA from different cancer cell lines was extracted using RNA isolation kit (Bio Rad) and treated with DNAse using DNAse 1 kit (AMPD1, Sigma-Aldrich). cDNA synthesis was done using a cDNA synthesis kit (Bio Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... bead-based kit (Milliplex 41-plex human cytokine panel 1, Millipore, MA, USA) on the FLEXMAP 3D system (Luminex Corporation ...
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bioRxiv - Immunology 2021Quote: ... 50 μl/well of 3,3’-5,5’-tetramethylbenzidine (TMB) Liquid Substrate System for ELISA (T040, Sigma-Aldrich Co. LLC., St. Louis, MO) was added to the plate ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Immunology 2020Quote: ... Absolute OVA-specific IgG1 concentration was determined for each sample by ELISA as described above using OVA-14 (Sigma, MO USA) as a standard and equalized across all samples ...
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Immunology 2019Quote: ... The resulting hybridomas were screened by ELISA for reactivity with STm whole cells and purified LPS (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Microbiology 2020Quote: ... The ELISA plates were washed 4 times by PBST and then 100 µL of 2000-fold diluted anti-strep-HRP (Millipore Sigma) were added into each well for another 1 hr at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Biotinylated IFX fragments were immobilised on a streptavidin-coated plate and binding of the six mouse monoclonal antibodies was tested by ELISA and detected with an alkaline-phosphatase-conjugated anti-mouse secondary antibody (Sigma-Aldrich) as previously described43 ...
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bioRxiv - Immunology 2022Quote: ... The HIV status of all participants was determined by fourth generation enzyme linked-immunosorbent assay (ELISA) testing and HIV RNA plasma viral load (Sigma Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... For application in ELISA elution fractions were pooled via Amicon Ultra-15 50k centrifugal filter units (Merck Millipore, Burlington, MA, USA). Elution buffer was chosen for the intended application (coating buffer for sandwich ELISA ...
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bioRxiv - Immunology 2023Quote: ... We confirmed the specificity of our IgA ELISA and non-cross reactivity toward IgG using purified human IgG and IgA (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... Plasma was collected from blood during the GTT to determine insulin levels by ELISA (EZRMI-13K, EMD Millipore Corporation, Burlington, US). Mice were sacrificed at day 7 after a 3h fast ...
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bioRxiv - Synthetic Biology 2023Quote: 50uL of Fc-PRL fusions or IgG1 isotype control were diluted in PBS to 2ug/mL were used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) and incubated overnight at 4c ...
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bioRxiv - Synthetic Biology 2023Quote: 50uL of Fc-PRL fusions or IgG1 isotype were control diluted in PBS to 2ug/mL were used to coat MaxiSorp 96-well ELISA plates (Sigma; M9410) and incubated overnight at 4c ...
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bioRxiv - Microbiology 2022Quote: ... The fraction of proteins >1 kDa was obtained by dialysis using the Mini Dialysis Kit 1 kDa (Sigma #GE80-6483-94). All kits were applied according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: RAS and RAC activity were measured using a RAF-1 RBD and PAK-1 PDB pull-down assay kits respectively (Cat#17218 and Cat#14325, Millipore Sigma) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Acetaldehyde content was further quantified using the supernatant of WT and ΔmftG grown in HdB-Tyl supplemented with 10 g L−1 ethanol alone and 10 g L−1 glucose combined with 10 g L−1 ethanol for 48 h as described in the acetaldehyde Assay Kit (Sigma-Aldrich) by the manufacturers.
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bioRxiv - Genomics 2021Quote: The wool cortisol concentration in each sample was determined by colourmetric analysis using polyclonal anticortisol antiserum (R4866 – supplier UC Davies California, USA) diluted in ELISA coating buffer (Carbonate-Bicarbonate Buffer capsule (Sigma C-3041) and 100 mL Milli-Q water ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Plasma insulin and C-peptide were measured with mouse/rat ELISAs (EZRMI-13K and EZRMCP2-21K, respectively, EMD Millipore, Billerica, MA, USA). Plasma non-esterified fatty acids (NEFA ...
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bioRxiv - Cell Biology 2021Quote: ... Insulin secreted from the islet grafts was measured in the mouse serum three weeks post-transplant using a human insulin ELISA (Millipore, EZHI-14K).
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bioRxiv - Physiology 2021Quote: Cortisol concentrations were measured in mouse sera by a solid phase competitive enzyme linked immunosorbent assay (ELISA) (Sigma-Aldrich Co Ltd, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... Plasma levels of counter-regulatory hormones and GHRH levels in cell supernatant were determined by ELISA (Crystal Chem Glucagon 81518; Corticosterone 80556; GH Millipore Sigma 3P EZRMGH45K ...
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bioRxiv - Neuroscience 2021Quote: The activation of Caspase 1 in mouse brain samples were determined using Caspase 1 Colorimetric Assay Kit (YVAD, Merck Millipore, Burlington, MA) and following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Final concentration was measured by Qubit™ RNA BR Assay Kit (Thermo Fisher Scientific. RNA integrity was assessed by gel electrophoresis with 1:1 formamide (Merck Millipore) and 1 µl of loading dye on 1.5% agarose gel ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We used an in–house ELISA in which the plate was coated with individual sera and biotinylated MS-Hu6 was captured by streptavidin-HRP (Millipore, Catalog # 18-152). In a final experiment ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 RBD (2.5 µg/mL) was adsorbed overnight at 4°C to a 384-well Nunc MaxiSorp ELISA plate (Millipore Sigma, St. Louis, MO). The RBD was removed via aspiration and the plate blocked with 3% BSA in TBST for 1 hour at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to N protein was detected using conventional two-step ELISA with an HRPO-conjugated anti-mouse or anti-rat Fc antibody (Sigma Aldrich cat # A9309). All assays included a standard curve of mouse neutralising S1 antibody and mouse anti-N antibody (Sino Biological) ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were screened using ELISA (see below) and antigen-reactive clones were expanded in serum IgG free RPMI-1640 (Sigma-Aldrich, MO, USA) into roller bottles (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... IL-31 and IL-33 cytokines with multiplexed-ELISA assay with Luminex 200 Multiplex Bio-Plex 200 System (EMD Millipore, Billerica, MA, USA) using a Milliplex Map kit (EMD Millipore).
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bioRxiv - Microbiology 2019Quote: ... The product was purified from 1% agarose gel with GenElute Gel Extraction Kit (Sigma-Aldrich) and cloned into pJET1.2/blunt vector using CloneJET PCR Cloning Kit (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... Serum insulin and c-peptide levels were measured by insulin or C-peptide ELISA respectively following the manufacture’s protocol (Sigma Aldrich, St. Louis, MO, USA). Mouse metabolic testing and fasting methods followed established protocols [16,33,34].
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bioRxiv - Immunology 2020Quote: Blood was withdrawn at the indicated times during high fat diet period and plasma lipid levels were enzymatically measured using ELISA (Sigma Aldrich, Zwijndrecht, the Netherlands).
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bioRxiv - Bioengineering 2022Quote: ... Peanut-specific IgG1 was detected using goat anti-mouse IgG1-HRP conjugated (Southern Biotechnology Associates) and substrate TMB liquid substrate system for ELISA (Sigma-Aldrich, St. Louis, MO). The plates were read in an ELISA plate reader at 405 nM (IgE ...
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bioRxiv - Cancer Biology 2022Quote: ... cell death detection enzyme-linked immunosorbent assay PLUS (ELISA) and human anti-β-actin antibody were obtained from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Poly-ADP-ribose polymerase (PARP ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was isolated from Nb-1 shoots with the Spectrum Plant Total RNA kit (Sigma-Aldrich) with on-column DNase I digestion (New England Biolabs) ...
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bioRxiv - Genetics 2020Quote: ... and diluted to measure protein concentration (1:10 diluted samples, Bradford Assay Kit, Sigma-Aldrich, B6916) and ATP level (1:2,000 diluted samples ...
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bioRxiv - Microbiology 2022Quote: Extraction method 1 used a modified protocol with the GenElute Bacterial genomic DNA kit (Sigma-Aldrich). The pellet was resuspended in 180 μl of kit-included lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: The transporter activity of MDR-1 was assessed using the MDR1 Efflux Assay Kit (ECM910, Millipore), as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Pop 9-12) (Figure 1) was isolated using the GenElute Bacterial Genomic DNA Kit (Sigma-Aldrich). DNA-purity and - quantity was assessed using a NanoDrop ND-1000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Genetics 2024Quote: ... Cell lysis took place in 1 ml of EZ-ChIP kit lysis buffer (Millipore, 17-371RF), and chromatin was sheared into approximately 1000 bp fragments via sonication using a Diagenode Pico Bioruptor ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated with primary antibodies (1:200) which were diluted with Immunoreaction Enhancer Kit (Millipore sigma) overnight at 4°C in a humid chamber ...
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bioRxiv - Cancer Biology 2023Quote: ... 350 μl RLT buffer (from the AllPrep DNA/RNA Micro Kit) containing 1% β-Mercaptoethanol (Sigma-Aldrich) was added to each sample (containing one biopsy ...
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bioRxiv - Genomics 2022Quote: ... The tissue was gently Dounce homogenized in 500 µL of Nuclei Isolation Buffer (Sigma Nuclei PURE Prep Kit: Lysis Buffer, 1 mM DTT, Sigma Nuclei PURE Prep Kit: 10% Triton X-100). After a brief centrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... The phosphatase assay was carried out using the Ser/Thr Phosphatase Assay Kit 1 (Millipore Sigma (MA, USA)) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: Nuclear and cytoplasmic extracts were prepared from differentiated THP-1 cells using Nuclei EZ Prep kit (Sigma-NUC101) both from un-stimulated and MprA stimulated cells ...