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Citations for Millipore Sigma :
401 - 450 of 6791 citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Cell Biology 2020Quote: The C-terminal tails of Taxol-stabilized Cy5 microtubules were cleaved using Subtilisin A (Sigma Aldrich) as previously described (72) ...
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bioRxiv - Immunology 2022Quote: ... The proteolytically cleaved peptides were extracted and desalted using ZipTip μC18 tips (Merck Millipore, Darmstadt, Germany). The peptide lysates were re-suspended in 15 µl 0.1% formic acid and analysed by nanoliquid chromatography mass spectrometry (UltiMate 3000 RSLCnano ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transferred to ice and surface biotin was cleaved with 60 mM MesNa (Sigma, 63705) in MesNa buffer (50 mM Tris-HCl [pH 8.6] ...
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bioRxiv - Biochemistry 2021Quote: ... The MBP-HapX fusion was cleaved with TEV protease and further purified sequentially using CellufineSulfate (Millipore) affinity chromatography ...
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bioRxiv - Biochemistry 2022Quote: ... Cleaved protein was concentrated in an Amicon Ultra-15 centrifugal filter units (30 kDA, Millipore #UFC9030) and passed through two subsequent gel filtration steps using a ...
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bioRxiv - Plant Biology 2021Quote: The inhibitory effect of recombinant P235 on recombinant glycosyl hydrolase was assayed using the β-Glucosidase Activity Assay Kit (MAK129, Sigma). Enzymatic reactions were carried out in K-Phosphate buffer (100 mM ...
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bioRxiv - Cancer Biology 2019Quote: ... polymerase (PARP)-1 polyclonal antibody (1:1000; Epitomics, Burlingame, CA, USA) and a mouse anti-β-actin antibody (1:10000; Sigma-Aldrich, St. Louis, MO, USA), followed by incubation with secondary antibodies and chemiluminescence detection.
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bioRxiv - Neuroscience 2020Quote: ... In the case of MBP antibody (Merck Millipore, Germany; Cat. No. MAB 386), after dilution at 1:50 with 0.2% Triton-X 100 for 20 min permeabilization ...
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bioRxiv - Cell Biology 2021Quote: ... mouse mAb anti-α-Tubulin (Sigma-Aldrich, clone B-5-1-2, #T5168); mouse mAb anti-Flag M2 (Sigma-Aldrich #F1804) ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... FLAG and Myc tagged-proteins were detected with mAb-FLAG M2 (Sigma-Aldrich) and mAb-Myc 1-9E10.2 (ATCC) ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... β-actin was detected with MAb β-actin AC-15 from Sigma-Aldrich. Horseradish peroxidase-conjugated anti-mouse/-rabbit secondary antibodies for Western blot analysis were obtained from DIANOVA GmbH (Hamburg ...
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bioRxiv - Cell Biology 2022Quote: ... and primary mouse anti-α tubulin mAb (Sigma-T9026; used at 1:1000). Secondary antibodies were Alexa 488 conjugated anti-mouse IgG (Invitrogen-A11004 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were counterstained with mouse monoclonal anti-NeuN (1:500; Millipore MAB-377). Following incubation with primary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... Anti-ICP4 MAb H1A021 or monoclonal Ab to VSV glycoprotein G (P5D4, Sigma) was added for 1 h followed by Alexa Fluor 488-labeled goat anti-mouse IgG (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... sLea and sLex antigens were detected with mAbs MEM-158 (Sigma-Aldrich France), 7-Le (Sigma-Aldrich France) ...
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bioRxiv - Cell Biology 2019Quote: ... and primary mouse anti-α tubulin mAb (Sigma-T9026; used at 1:1000). Secondary antibodies were Alexa 488 conjugated anti-mouse IgG (Invitrogen-A11004 ...
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bioRxiv - Microbiology 2019Quote: ... Mouse HRP-conjugated anti-actin mAb (1:35,000) (clone AC-15, Sigma-Aldrich) was used as loading control.
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bioRxiv - Neuroscience 2020Quote: Sections were incubated overnight at 4 °C with RHO mAb (Sigma-Aldrich, MAB5316). Fluorescence immunocytochemistry was performed using Alexa Fluor® 568 conjugated secondary antibody (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... and interneurons with GAD 67 (anti-mouse; Millipore, MAB 5406; 1:500 dilution), GAD 65/67 (anti-rabbit ...
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bioRxiv - Cell Biology 2019Quote: ... and primary mouse anti-α tubulin mAb (Sigma-T9026; used at 1:1000). Secondary antibodies were Alexa 488 conjugated anti-mouse IgG (Invitrogen-A11004 ...
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bioRxiv - Cell Biology 2021Quote: Sections were incubated overnight at 4 °C with rhodopsin mAb (Sigma-Aldrich, MAB5316). Fluorescence immunocytochemistry was performed using Alexa Fluor® 568 conjugated secondary antibody (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... and primary mouse anti-α tubulin mAb (Sigma-T9026; used at 1:1000) or mouse anti-centrin mAb (Millipore-04-1624 ...
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bioRxiv - Cell Biology 2020Quote: ... and 1:1000 dilution mouse anti-α-tubulin mAb (Sigma-Aldrich, Cat. # T5168). Subsequently ...
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bioRxiv - Developmental Biology 2022Quote: ... mouse mAb to α-SMA conjugated to Cy3 (Clone 1A4, Sigma, 1/300), mouse mAb to βIII-tubulin conjugated to Alexa488 (Clone Tuj-1 ...
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bioRxiv - Microbiology 2022Quote: ... IE2 was detected using mouse monoclonal antibody (mAb) clone 5A8.2 (MAB8140; Millipore, Inc), GAPDH was detected using mouse mAb 60004-1-Ig (Proteintech) ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with the following primary antibodies: anti-FLAG (1:1,000; Sigma; Mab), anti-ubiquitin (1:10,000 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-FLAG-tag (1:5,000, mouse mAb, F1804, Sigma-Aldrich, St. Louis, MO), anti-alpha-tubulin (1:2,000 ...
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bioRxiv - Cell Biology 2023Quote: ... and primary mouse anti-α tubulin mAb (Sigma-T9026; used at 1:1000). Secondary antibodies were Alexa 488 conjugated anti-mouse IgG (Invitrogen-A11004 ...
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bioRxiv - Cell Biology 2023Quote: ... Mouse anti-Golgi 58K protein (formimidoyltransferase-cyclodeaminase) mAb (G2404) was from Sigma-Aldrich and used at a dilution of 1:50∼100 ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with 1 μg/mL anti-Flag mAb (Sigma, F1804) or anti-β-tubulin (Immmuno Way ...
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bioRxiv - Cell Biology 2024Quote: ... phospho-p70 S6 Kinase (Thr389) (Cat. No. MAB S82, Millipore Sigma, Burlington, MA) GAPDH (Cat ...
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bioRxiv - Microbiology 2021Quote: Poly-histidine tags were cleaved from proteins by treating them with 3 units of TEV protease (Sigma) overnight at 4 °C based on the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... The TEV cleaved gB was eluted from the beads with TBS pH7.4 containing 1mg/ml lauroylsarcosine (Sigma) and 1mg/ml Amphipol 8-35 (Anatrace) ...
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bioRxiv - Neuroscience 2021Quote: ... Remaining surface biotin was cleaved by incubation with glutathione cleavage buffer (50 nM L-Glutathione (Sigma #G6013) 75 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... After eluting and concentrating the cleaved protein in Amicon Ultra-15 Centrifugal Filters 50K MWCO (Merck Millipore), size exclusion chromatography (SEC ...
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bioRxiv - Immunology 2024Quote: ... The digestion was stopped and the surfactant was cleaved by treating samples with 200 mM HCl (Sigma) at 37°C for 30 minutes ...
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bioRxiv - Physiology 2021Quote: ... with 0.75 U/kg of recombinant insulin (Sigma-Aldrich). Blood samples were collected from the tail vein and glucose was measured using a glucometer (Roche ...
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bioRxiv - Genetics 2021Quote: ... 10 μg ml-recombinant human insulin (Sigma, 19278-5ML), 2 IU ml-heparin (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: ... 1000 U/mL recombinant leukemia inhibitory factor (LIF, Millipore), and 0.3% BSA (Gibco ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Cell Biology 2021Quote: ... active recombinant GSK3α was purchased (Millipore, Cat# 14-492). 0.8 µg Dyn2WT or Dyn2S848A was incubated with 10 ng GSK3α in kinase buffer (50 mM HEPES (pH 7.4) ...
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bioRxiv - Biophysics 2020Quote: ... ErbB2 (Her2) human recombinant was purchased from Sigma-Aldrich. Deglycosylated trastuzumab was obtained by treatment with Endo S (New England BioLabs ...
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bioRxiv - Cell Biology 2021Quote: ... 10 nM recombinant human [Leu15]-gastrin I (Sigma G9145), 50 ng/ml recombinant mouse EGF (Peprotech ...
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bioRxiv - Cell Biology 2021Quote: ... active recombinant GSK3α was purchased (Millipore, Cat# 14-492). 0.8 µg Dyn2WT or Dyn2S848A was incubated with 10 ng GSK3α in kinase buffer (50 mM HEPES (pH 7.4) ...
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bioRxiv - Neuroscience 2020Quote: ... brefeldin A and recombinant insulin were purchased from Sigma. NSC23766 was purchased from Santa Cruz ...
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bioRxiv - Bioengineering 2022Quote: ... Plates were coated with either recombinant GFP protein (Millipore) at 1 μg/mL to measure GFP-specific responses ...
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bioRxiv - Genomics 2019Quote: ... 100 U/mL recombinant human IL-2 (Sigma-Aldrich) and 1% penicillin-streptomycin (final concentration 100 U penicillin ...
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bioRxiv - Microbiology 2022Quote: ... recombinant IL-2 (100 U/mL; Sigma Millipore #11011456001), and anti-CD28 + anti-CD49d (1.5ug/ml ...