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Citations for Millipore Sigma :
251 - 300 of 1716 citations for Chikungunya virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were incubated with anti-Flag M2 agarose (Sigma) at 4°C at least 6 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were incubated with either IgG (anti-rabbit IgG, Sigma) or GFP-nanobody (expressed from pDZ580-pET28a-GBP and purified as described in-conjugated magnetic beads (Dynabeads M-280 ...
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bioRxiv - Plant Biology 2020Quote: ... The nuclei lysate was precipitated with anti-Flag (Sigma-Aldrich), anti-Myc (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were incubated with Anti-FLAG M2 Magnetic Beads (Sigma), washed to remove unbound proteins ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were then incubated with glutathione-agarose resin (Sigma-Aldrich) for 2 hours rotating at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: Cell lysates were prepared in RIPA buffer (Sigma Aldrich # R0278) supplemented with dithiothreitol (Life Technologies # 15508013) ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell lysates were prepared with RIPA buffer (R0278, Sigma) containing protease (cOmplete ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were precleared with protein G-sepharose 4B beads (Sigma) and then incubated with anti-HA antibody (HA.11 ...
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bioRxiv - Cancer Biology 2022Quote: Cell lysates were collected by processing with RIPA buffer (Sigma), and protein concentrations were quantified using a BCA kits (Pierce Chemical) ...
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bioRxiv - Microbiology 2022Quote: ... purified high titer lysates were treated with Benzonase Nuclease (Sigma) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... lysates were quantified for normalisation (BCA Protein Assay kit, Millipore) and loaded on 10% denaturing SDS-polyacrylamide gels ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysates were then incubated with Flag M2 beads (Sigma), anti-HA affinity matrix (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... Cleared lysate was applied to a pre-equilibrated HisTrap (Sigma) Ni-sepharose column and washed with 10 column volumes wash buffer I (50mM NaH2PO4 ...
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bioRxiv - Neuroscience 2021Quote: ... The lysate was then treated with Benzonase nuclease HC (Millipore) for 45 minutes at 37 oC ...
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bioRxiv - Neuroscience 2021Quote: ... Lysates were treated with benzonase (200 U/mg protein, Sigma) for 5 min and then clarified by centrifugation at 15,000 g for 10 min ...
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bioRxiv - Molecular Biology 2022Quote: Protein lysates were generated by incubation with RIPA Buffer (Sigma) supplemented with 1% complete protease inhibitor cocktail (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... and cell lysates were immunoprecipitated with Flag beads (Sigma Aldrich) overnight at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were immunoprecipitated using EZview anti-HA Affinity Gel (Sigma) or anti-Flag Affinity Gel (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... Clarified lysates were incubated with FLAG M2 magnetic beads (Sigma) for 1 hour ...
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bioRxiv - Systems Biology 2019Quote: ... the lysate was incubated with anti-FLAG agarose resin (Sigma) at 4°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... cell lysates were incubated with 200 µM PpIX (Sigma Aldrich) in 200 µL assay buffer (0.1 M Tris-HCl ...
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bioRxiv - Systems Biology 2019Quote: Cell lysate was extracted with CelLytic M (C2978, Sigma-Aldrich) lysis buffer ...
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bioRxiv - Cancer Biology 2021Quote: Cell lysates were prepared using RIPA buffer (#20-188, Millipore) supplemented with protease and phosphatase inhibitors (#A32959 ...
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bioRxiv - Immunology 2020Quote: ... Cell lysates were incubated with anti-FLAG beads (Sigma Aldrich) conjugated with 10 μg FLAG-tagged rGal9 ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Solubilized lysate was immunoprecipitated using M2-Flag-agarose conjugate (Sigma). Protein samples were separated using 10% polyacrylamide gels (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was filtered with a 0.22 µM filter (Millipore) to remove cellular debris and the protein was column-purified using anti-histidine resin (ClonTech) ...
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bioRxiv - Microbiology 2021Quote: The lysates were passed through a 0.22 μm filter (Millipore) to remove any other aggregates or insoluble particles ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysates were incubated with anti-FLAG M2 resin (Sigma) for overnight with nutation ...
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bioRxiv - Biochemistry 2021Quote: ... the lysate was loaded onto a Ni2+-affinity resin (Sigma), washed with high salt (800 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... Soluble lysates were incubated with GST-Bind resin (EMD Millipore) for 30 min at 4 °C ...
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bioRxiv - Microbiology 2021Quote: Total cell lysates were prepared using the RIPA buffer (Sigma) containing protease inhibitors (Roche) ...
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bioRxiv - Microbiology 2021Quote: Cell lysate was extracted by incubating in lysis buffer (Sigma) with protease inhibitor (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... The crude lysate was treated with 250 U benzonase (Sigma) per 1 ml of lysate at 37°C for 1.5 h to degrade genomic and unpackaged AAV DNA before centrifugation at 3,000 g for 15 min to pellet cell debris ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were filtered through a 0.22 μm syringe filter (Millipore), and further washed or concentrated using Amicon Ultra MWCO 100 kDa centrifugal filter units (Millipore) ...
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bioRxiv - Cell Biology 2022Quote: Lysates were generated by incubating cells in RIPA buffer (Millipore). 20-30 μg of cleared lysate were analyzed by SDS-PAGE as previously described (25) ...
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bioRxiv - Developmental Biology 2022Quote: ... The cell lysates were precleared with IgG-agarose beads (Sigma) for 8 hours at 4°C.
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bioRxiv - Neuroscience 2022Quote: ... The lysate was washed and concentrated with Amicon Filter (Millipore) to achieve at least 2.0 x 1012 gc/ml ...
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bioRxiv - Physiology 2022Quote: ... Liver lysates were analyzed by immunoblotting using anti-Prox1 (Millipore - 07-537 and Abcam - ab38692 in Fig ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein lysates were diluted with Laemmli sample buffer (Sigma Aldrich) and distilled water ...
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bioRxiv - Cell Biology 2023Quote: ... cell lysates were pre-cleared with 4B Sepharose beads (Sigma) at 4°C for 1 h while rocking ...
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bioRxiv - Microbiology 2023Quote: Cells lysates were prepared in Radioimmunoprecipitation assay buffer (RIPA) (Sigma) supplemented with Protease Inhibitor Cocktail (G biosciences) ...
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bioRxiv - Cell Biology 2023Quote: Whole-cell lysates were prepared with RIPA lysis buffer (Millipore) containing cOmplete EDTA free protease inhibitor cocktail (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified from lysate using either nickel (Sigma-Aldrich) or glutathione resin (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... and whole-protein lysate was extracted using Bugbuster reagent (Novagen) and Pierce Universal Nuclease according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Total lysates were incubated with anti-FLAG beads (Sigma, USA) or monoclonal anti-GFP antibody (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... Cleared lysates were filtered through 0.45 μM spin filters (Millipore) and immunoprecipitated with anti-FLAG antibody-conjugated M2 agarose (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cleared lysates were filtered through 0.45 μM spin filters (Millipore) and immunoprecipitated with Strep-Tactin beads (IBA lifescience) ...
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bioRxiv - Molecular Biology 2023Quote: ... Clarified lysate was filtered with a 0.22 µm filter (Millipore). The supernatant was loaded on to a 30% sucrose cushion (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... lysates were filtered using an Amicon Ultra 0.5 (Sigma-Aldrich) and a table top centrifuge (30min at 4°C and maximum speed) ...
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Phospho-KNL-1 recognition by a TPR domain targets the BUB-1–BUB-3 complex to C. elegans kinetochoresbioRxiv - Cell Biology 2024Quote: ... Cell lysates were incubated with M2 anti-FLAG beads (Sigma) while rotating for 2 hours at 4°C ...