Labshake search
Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: ... recombinant ephrins or Fc were preclustered in a 5:1 molar ratio with either mouse or goat anti-human Fc (Millipore Sigma cat. no. 16760 and 12136 respectively) for 30’ at 37°C.
-
bioRxiv - Immunology 2020Quote: NTD-Fc or RBD-Fc proteins were diluted in sterile PBS and formulated as an emulsion with TiterMax Gold adjuvant (Sigma-Aldrich, St. Louis, MO) to a final antigen concentration of 250 µg/mL ...
-
bioRxiv - Cancer Biology 2022Quote: Expression of indicated Flag-tagged CDK6 constructs in HEK293 cells were determined via Western blotting with indicated Flag antibody [mouse anti-FLAG® M2-tag (Sigma-Aldrich, St. Louis, MO, USA, F3165-1MG)] ...
-
bioRxiv - Cell Biology 2022Quote: ... or E2+DFP (80 mg/kg daily, Sigma–Aldrich, St. Louis, MO) for eight weeks from week nine as the early OVX group or week 21 as the late OVX group.
-
bioRxiv - Genomics 2022Quote: ... fresh media containing 10 nM E2 and 1 µM medroxyprogesterone acetate (Sigma) was added ...
-
bioRxiv - Developmental Biology 2022Quote: ... we treated the mutant fish with E2 (Sigma-Aldrich, St. Louis, MO) followed by sampling for histological analysis ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 10-8M 17-ß-estradiol (E2, Sigma-Aldrich, Saint-Louis, USA) and were kept at 37°C with 5% CO2 in a humidified atmosphere ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 10-8M 17-ß-estradiol (E2, Sigma-Aldrich, Saint-Louis, USA) and were kept at 37°C with 5% CO2 in a humidified atmosphere ...
-
bioRxiv - Immunology 2022Quote: ... cells were treated with 10 nM of 17β-estradiol (E2; Sigma-Aldrich) and collected after a 36 hr incubation according to previously established protocols [21].
-
bioRxiv - Developmental Biology 2024Quote: ... The E2/P4 mice received 1 mg of progesterone (P4; Sigma-Aldrich) for three consecutive days followed by a single injection of 1 mg P4 plus 50 ng E2 on the fourth day ...
-
bioRxiv - Bioengineering 2020Quote: ... or human hemoglobin (all proteins were purchased from Sigma). Protein nanorods were prepared using chemically modified human serum albumin.
-
bioRxiv - Microbiology 2020Quote: ... cells were stained with 1/200 diluted Goat Anti human IgG Fc-FITC antibody (Cat: F9512, Sigma) for 45 min and analyzed using flow cytometry (CytoFLEX ...
-
bioRxiv - Molecular Biology 2020Quote: ... 10% FCS and human fibroblast growth factor-2 (5 ng/mL, Sigma-Aldrich, St Louis, MO, USA) at 37 °C ...
-
bioRxiv - Bioengineering 2021Quote: ... and binding was detected with an anti-human IgG (Fc specific) (1:5000 dilution, Sigma-Aldrich, A0170) or anti-FLAG (1:1000 dilution ...
-
bioRxiv - Bioengineering 2023Quote: ... Antibodies and dyes used in this study were: Anti-Human IgG Fc specific (Millipore Sigma I2136-1ML), Anti-6X His tag (Abcam ab9108) ...
-
bioRxiv - Biochemistry 2020Quote: ... FLAG tag (F1804) or His tag (H1029) were from Sigma. Mouse monoclonal antibody raised against hemagglutinin (HA ...
-
bioRxiv - Cancer Biology 2020Quote: Vesicular stomatitis virus (VSV)-G protein pseudotyped lentiviruses and retroviruses were prepared by using polyethylenimine (PEI; Sigma) to co-transfect 293T cells with a lentiviral or retroviral plasmid and pantropic VSVg (Addgene ...
-
bioRxiv - Immunology 2020Quote: ... FCS (Sigma Life Science ...
-
bioRxiv - Biophysics 2021Quote: HEK293 cells were cultured in DMEM (Sigma) containing 10% FBS (Life Technologies) ...
-
bioRxiv - Cell Biology 2019Quote: HEK293 cells were cultured in DMEM (Sigma) with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Genomics 2022Quote: ... HEK293 cells were passaged in DMEM (Sigma) supplemented with 10% FBS (Gibco ...
-
bioRxiv - Cancer Biology 2023Quote: ... envelope plasmid and 130 μL 1 μg / mL polyethylenimine (Sigma-Aldrich) in 450 μL Opti-MEM media (Lonza) ...
-
bioRxiv - Cell Biology 2021Quote: ... Flag-tag (Sigma), Myc-tag (Upstate) ...
-
bioRxiv - Cell Biology 2024Quote: Doxycycline-inducible stable Flp-In T-REx HEK293 cell lines expressing mitochondrial-relocated golgin-BirA* fusion proteins were induced with 1 μg/ml doxycycline (Sigma) in culture medium once they reached approximately 80% confluency ...
-
bioRxiv - Microbiology 2022Quote: ... varicella-zoster virus (Light Diagnostics, Varicella-Zoster Virus DFA kit, EMD Millipore, Temecula, CA), adenovirus (D3Ultra Respiratory Virus Screening & Identification kit ...
-
bioRxiv - Biochemistry 2022Quote: ... and the GST tag was cleaved on column by incubating the protein with Thrombin (Sigma Aldrich) overnight at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The proteins were eluted by protease cleavage of the GST-tag (thrombin or PreScission (Sigma-Aldrich) were used depending on the enzyme availability ...
-
bioRxiv - Molecular Biology 2021Quote: ... Target proteins were detected using the following antibodies: mouse anti-Strep tag II (Millipore Sigma: 71590); rabbit anti-Caspase 3 (Cell signaling ...
-
bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
-
bioRxiv - Bioengineering 2019Quote: ... tissue culture polystyrene culture plates were pre-coated with 20 mg/ml anti-IgG (human, Fc-specific, Sigma) for 1 hour at 37°C ...
-
bioRxiv - Microbiology 2020Quote: ... We detected bound antibody with horseradish peroxidase (HRP)-coupled anti-human (Fc) antibody (Sigma Aldrich catalog number A0170).
-
bioRxiv - Cell Biology 2020Quote: ... EMOs were cultured in the presence of 17-β-estradiol (E2; Sigma-Aldrich) for four days.
-
bioRxiv - Molecular Biology 2019Quote: ... cells were treated with 100 nM β-estradiol (E2, ethanol solution, Sigma-Aldrich) or an equivalent volume of ethanol ...
-
bioRxiv - Cancer Biology 2020Quote: ... and 17-β estradiol (E2) were purchased from Millipore Sigma (St. Louis, MO). For in vitro hormone treatments ...
-
bioRxiv - Immunology 2022Quote: ... cells were treated for 24h with E2 (Sigma-Aldrich; Cat: E1024; Batch: SLCC8875), S (R&D Systems ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were stimulated with estradiol (E2, 10 nM, Sigma-Aldrich, Saint Louis, USA) or vehicle (DMSO ...
-
bioRxiv - Cancer Biology 2020Quote: ... and purified human GST protein (5 mg/ml) (Sigma-Aldrich) were incubated for 30min with biotinylated probes (100 pM ...
-
bioRxiv - Neuroscience 2020Quote: Human Embryonic Kidney Cells (HEK293) and HEK293 Flp-In T-Rex cells were grown and maintained in Dulbecco’s modified Eagle medium (DMEM, Sigma-Aldrich) supplemented with 10% (v/v ...
-
bioRxiv - Cell Biology 2022Quote: ... HEK293 FlpIn mycBirA*FOPNL and HEK293 FlpIn mycBirA* cells were incubated for 24 h with 50 μM biotin (Sigma-Aldrich) in the presence or absence of 0.1 μg/ml tetracycline.
-
bioRxiv - Microbiology 2019Quote: ... 1.8 μg of pRSV-Rev and 1.5 μg of plasmid containing pAIP-NSP2-mCherry or pAIP-NSP5 and the human immunodeficiency virus long terminal repeats were co-transfected with Lipofectamine 3000 (Sigma-Aldrich) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2019Quote: ... and HEK293 and HEK293T cells in DMEM (Sigma) containing 10% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2020Quote: ... and HEK293 cells with polyethylenimine (PEI, Sigma Aldrich). Details are described in the SI Appendix ...
-
bioRxiv - Neuroscience 2022Quote: HEK293 culture (ECACC 85120602, Sigma-Aldrich, Munich, Germany), transfection and electrophysiological recordings were performed as described previously (Grimm et al. ...
-
bioRxiv - Biophysics 2023Quote: HEK293 cells were cultured in RPMI medium (Sigma) supplemented with 10% v/v fetal bovine serum (FBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were treated with MG132 (Sigma-Aldrich) at a final concentration of 1 or 10 µM ...
-
bioRxiv - Bioengineering 2021Quote: ... Four different target Fc-proteins were used in this study: hIgG (Sigma-Aldrich, St. Louis, MO), plant-expressed rCMG2-Fc ...
-
bioRxiv - Cell Biology 2023Quote: ... Secreted JAGGED1-Fc was recovered from the culture media on Protein A agarose (Millipore, 16-125) and eluted with 100 mM glycine ...
-
bioRxiv - Microbiology 2021Quote: Poly-histidine tags were cleaved from proteins by treating them with 3 units of TEV protease (Sigma) overnight at 4 °C based on the manufacturer’s guidelines ...
-
bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
-
bioRxiv - Cell Biology 2020Quote: ... Recombinant Py-S2 proteins fused to hexa-histidine tag were expressed in Rossetta2 (DE3) (EMD Millipore/Novagen) and purified with hexa-histidine affinity resin (Talon beads from Takara/Clontech) ...