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Citations for Millipore Sigma :
351 - 400 of 10000+ citations for Centrosomal Protein 63 kDa CEP63 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... All antibodies used for electroporation were passed through Amicon Ultra-0.5 100 kDa centrifugal filter devices (Millipore) to remove traces of azide and replace buffer with PBS ...
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bioRxiv - Immunology 2023Quote: ... The reduced antibody was washed in C buffer in 50 kDa Amicon® spin filters (Merck Millipore) before being mixed with the preloaded X8 polymer and incubated for 60-90 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... antibodies (100 μg) underwent buffer exchange to PBS via 50-KDa Amicon Ultra Centrifugal Filters (Millipore, UFC505096) and were treated with 10 equivalents of PEGylated SMCC cross-linker (SM(PEG)2 ...
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bioRxiv - Molecular Biology 2019Quote: ... The 63 nucleotides long HSV thymidine kinase minimal promoter (TK mini) synthesized by SIGMA-ALDRICH, was added upstream of luciferase gene ...
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bioRxiv - Genomics 2023Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated using 63% Percoll (Sigma, Cytiva;17-0891-01) solution ...
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bioRxiv - Biophysics 2023Quote: ... and several milliliters of 0.01% Poly-L-Lysine (CAS Number 25988-63-0, p4707 Sigma) were added to the surface immediately following the plasma exposure ...
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bioRxiv - Cell Biology 2024Quote: ... they were treated with 10 μl of poly-L-lysine (Cat# 25988-63-0, Sigma) and incubated in a cell-culture incubator (37 °C and 8% CO2 ...
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bioRxiv - Plant Biology 2019Quote: ... The purified protein was concentrated using an Amicon Ultra centrifugal filter with a molecular mass cut-off (MWCO) of 30 kDa (Millipore) and analysed on a Superdex 200 size-exclusion chromatography (SEC ...
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bioRxiv - Synthetic Biology 2022Quote: −80 °C glycerol stocks of secreted proteins were thawed in a water bath and proteins were buffer exchanged using 0.5 mL 30 kDA molecular weight cutoff devices from Millipore Sigma at 4 °C into sterile water ...
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bioRxiv - Biophysics 2020Quote: ... The protein was then concentrated to 500 μL using a 50 kDa cutoff Amicon Ultra-4 Centrifugal filter (Millipore UFC805008) and injected onto a Superdex 200 increase gel filtration column in sizing buffer (25 mM HEPES pH 7.0 ...
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bioRxiv - Genetics 2020Quote: ... Proteins were concentrated using an Amicon Ultra-0.5 mL centrifugal filter device with a cutoff of 100 kDa (Millipore Sigma).
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing the protein were pooled and concentrated with an Amicon Ultracell Centrifugal filter unit (MWCO 50 kDa, Merck Millipore) to a volume of 500 µL and digested with TEV protease for 2 hours at 8°C ...
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bioRxiv - Microbiology 2020Quote: Fractions containing the DnaA-6xHis protein were pooled together and applied to Amicon Ultra Centrifugal Filters with 30 kDa cutoff (Millipore). Buffer was exchanged to Buffer A (25 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Residual methylation reagents were neutralised by addition of 1M Tris-HCl pH 7.5 to a final concentration of 50 mM Tris-HCl and the protein complex was concentrated to 2 mg mL−1 using 30 kDa cut-off concentrators (Millipore) before injection into a S75 16/600 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... PKG Iβ (71-686) protein was concentrated to 20 mg ml-1 by using 30 kDa cutoff Amicon Ultra (Millipore) and initial crystal screening was performed by Mosquito Crystal robot (TTP Labtech ...
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bioRxiv - Biochemistry 2022Quote: ... The purified proteins were concentrated using a prewashed Amicon Ultra-10 concentrator (molecular weight cut off 30 kDa; EMD Millipore), flash frozen in liquid N2 and stored in -80°C.
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bioRxiv - Biochemistry 2022Quote: ... The purified protein was concentrated using a prewashed Amicon Ultra-10 concentrator (molecular weight cut off 10 kDa; EMD Millipore), flash frozen in liquid N2 and stored in -80°C.
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bioRxiv - Immunology 2022Quote: ... 3 mM 2-mercaptoethanol and the mixture was concentrated to 10 mg/ml (Protein concentration) using an Amicon Ultra-4 filter (3 kDa molecular-weight cutoff, Millipore). The FoxP3ΔN’–DNA complex was then mixed with the reservoir solution (0.1 M Tris-Hcl pH 8.5 ...
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bioRxiv - Biophysics 2022Quote: ... Protein was concentrated to 30-40 mg ml-1 using a 50-kDa cutoff Amicon Ultra centrifugal filter unit (Millipore). NpSxph crystals were obtained by hanging drop vapor diffusion at 4°C using 1:1 v/v ratio of protein and precipitant ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the recombinant protein were pooled and concentrated through 15 mL 10-kDa MWCO Amicon-Ultra centrifugal filters (Millipore). Protein concentrations were measured by absorbance at 280 nm in nanodrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... and fractions containing protein of the appropriate size were pooled and concentrated using 10 kDa Amicon Ultra-0.5 Centrifugal Filter Units (Millipore, UFC505024) into buffer without imidazole (20 mM Tris-HCl pH = 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein fractions were concentrated to ∼12 mL using an Amicon Ultra-50 centrifugal filtration device (100-kDa MW cutoff, Millipore) and loaded onto a HiPrep Desalting column 26/10 (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... The protein was concentrated to 15 mL using an Amicon Ultra-15 centrifugal filtration device (50-kDa MW cutoff, Millipore). The protein was diluted with a modified buffer A (8% (vol∕vol ...
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bioRxiv - Biophysics 2021Quote: ... The fractions of the flow-through containing MSP protein were pooled together and concentrated with a 10 kDa Amicon Ultra-filter (Millipore) to 100-200 μM ...
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bioRxiv - Biophysics 2022Quote: ... Protein was buffer exchanged into phosphate buffered saline (PBS) containing 10% glycerol using a spin concentrator (10 kDa MWCO, Millipore).
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bioRxiv - Systems Biology 2020Quote: ... 200 μg of protein was then buffer exchanged with 50 mM ammonium bicarbonate with centricon filters (Millipore, 30 kDa cutoff) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The purified protein was then left at room temperature overnight to facilitate protein folding and chromophore maturation before concentrating and buffer exchanging into 1× TBS using 10 kDa centrifugal filter units (Millipore). Unless noted otherwise ...
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bioRxiv - Plant Biology 2021Quote: ... Then the proteins were concentrated to 10 mg/ml using the 30 kDa cut-off Amicon Ultra-4 centrifugal filter unit (Millipore), and stored at −80 °C for crystallization.
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bioRxiv - Immunology 2020Quote: ... M and N proteins were concentrated and buffer changed to phosphate buffered saline (PBS) using Microcon-10 KDa centrifugal filter units (Millipore). For N-terminal (residues from 43 to 180 ...
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bioRxiv - Immunology 2021Quote: ... Fractions containing the proteins were pooled and concentrated to ~ 8 mg/mL each with 30 kDa cutoff ultrafiltration units (Millipore). Time-resolved fluorescence resonance energy transfer (TR-FRET ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... GGG-azide labelled proteins were washed with Tris/NaCl buffer and concentrated to 25 µM on a 30 kDa Amicon Tube (Merck Millipore) and afterwards labelled with 100 µM DBCO-Cy5 (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... measuring its ability to prevent heat denaturation of two protein substrates: pig citrate synthase (CS) (0.2 μM; MW 51 kDa; Sigma Aldrich), and catalase purified from bovine liver (2 μM ...
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bioRxiv - Biochemistry 2022Quote: ... The MTF1 protein eluent was collected and concentrated using 10 kDa MW cut-off Amicon ultra-centrifugal filter (Merck Millipore) and stored at -80°C ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was then concentrated to ~200 μL with a 100 kDa molecular weight cutoff (MWCO) Amicon Ultracentrifugal filter (Millipore Sigma) at 1000 ×g ...
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bioRxiv - Plant Biology 2022Quote: ... Protein samples were concentrated by centrifugation using spin columns (MW cut off of 10 kDa) (cat number UFC501096– Merck Millipore). Protein concentration was estimated by UV using calculated extinction coefficients (42,400 mol/l-1.cm-1) ...
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bioRxiv - Molecular Biology 2022Quote: ... the eluted protein is pooled and concentrated to ∼40 mg/mL by using a 50 kDa cutoff ultrafiltration unit (Millipore). Finally ...
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bioRxiv - Microbiology 2023Quote: Eluted proteins were concentrated and desalted with an Amicon® Ultra-4 Centrifugal Filter Device (30 kDa MWCO, Millipore Sigma), with equilibration in protein binding buffer lacking imidazole ...
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bioRxiv - Synthetic Biology 2023Quote: ... Dialyzed protein samples were concentrated using Amicon Ultra centrifugal filter units with a 10 kDa molecular weight cutoff (Sigma-Aldrich).
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bioRxiv - Biochemistry 2023Quote: ... The next day the proteins were collected and concentrated using 30 kDa molecular weight cut-off centrifugal filters (Merck Millipore) for size exclusion chromatography.
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bioRxiv - Immunology 2023Quote: ... Protein fractions containing pure Cbl-b protein as confirmed by SDS-PAGE were pooled and concentrated using 10 kDa cutoff spin columns (Millipore), and the final protein concentration determined using the NanoDrop UV-Vis spectrophotometer (Thermo Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... Fractions that were supposed to contain the protein of the expected size were concentrated by ultrafiltration (10 kDa, UFC9010, Millipore), and aliquots were frozen at -20 °C until use.
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bioRxiv - Biochemistry 2023Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Biochemistry 2024Quote: ... The nhTMEM16 protein peak was collected and concentrated using a 100 kDa molecular weight cut off concentrator (Amicon Ultra, Millipore). The N-terminal GFP-His10 tag was cleaved overnight with 3C protease before gel filtration chromatography by using a Sepharose 6 Increase 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... The final protein was concentrated to ∼10 mg ml-1 using a 30 kDa molecular weight cut-off concentrator (Amicon Ultra, Millipore). The concentrated protein was flash-frozen and stored at −80 °C until further use.
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bioRxiv - Biochemistry 2024Quote: ... Imidazole was removed from the purified protein by serial buffer exchange in 10 kDa MWCO spin columns (Millipore Sigma, UFC801096). For CD44-Fc constructs ...
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bioRxiv - Biophysics 2024Quote: The purified protein was concentrated to 5–8 mg/ml using a 50 kDa molecular weight cutoff (MWCO) centrifuge concentrator (Millipore) and loaded in 0.5 mL portions onto a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences ...
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bioRxiv - Neuroscience 2021Quote: ... Antibody bound DNA-protein complexes were isolated by protein G plus/protein A agarose beads (EMD Millipore), washed and eluted ...
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bioRxiv - Biochemistry 2022Quote: ... concentrated by ultrafiltration on 10 kDa or 30 kDa molecular weight cut-off centrifugal devices (Millipore), and centrifuged at 21,500 g and 4 °C for 30 min to remove any aggregates ...
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bioRxiv - Molecular Biology 2020Quote: ... 30 kDa MWCO (Millipore/MERCK UFC903024) filters ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated (30 kDa Amicon filter; Millipore), and applied to a HiLoad 26/60 Superdex 200 size-exclusion column (GE Healthcare ...