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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 16HBE cells were grown on 8-well glass slides (Nunc Lab-Tek II chamber slide system; Sigma-Aldrich) with each well seeded with 450 µl of cell suspension at 1.2 x 105 cells/ml and grown for 24 hours ...
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bioRxiv - Biochemistry 2022Quote: ... The EC1-2 construct was cloned into pET21a plasmid system and transformed into BL21 (DE3) competent cells (Novagen). Cultures were grown in autoinduction media (PMID ...
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bioRxiv - Biophysics 2022Quote: ... The EC1-2 construct was cloned into pET21a plasmid system and transformed into BL21 (DE3) competent cells (Novagen). Cultures were grown in autoinduction media 24 overnight and harvested via centrifugation ...
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bioRxiv - Genetics 2020Quote: ... Enrichment of the chloroplast from the leaf samples was performed using ± 2 cm2 of leaf tissue with the Chloroplast Isolation Kit (Sigma, CPISO-1KT After this the DNA was extracted using a modified C-Tab protocol (Doyle JJ ...
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bioRxiv - Developmental Biology 2021Quote: ... L3 larvae or pupae of different stages) or tissues (adult head, thorax or abdomen or IFMs) were collected for RNA isolation using the TRI reagent (Sigma) based protocol ...
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bioRxiv - Cell Biology 2021Quote: ... after which we incubated a 20µL pre-isolation aliquot of the filtrate with 0.1µg/mL 4′,6-diamidino-2-phenylindole (DAPI) (Millipore-Sigma, cat. #D9542) and mounted on a slide for imaging (Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... after which we incubated a 20µL pre-isolation aliquot of the filtrate with 0.1µg/mL 4′,6-diamidino-2-phenylindole (DAPI) (Millipore-Sigma, cat. #D9542) and mounted on a slide for imaging (Fisher ...
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bioRxiv - Plant Biology 2020Quote: ... Arabidopsis root nuclei were isolated by chopping the roots in 1 ml of Nuclear Isolation Buffer (CELLYTPN1-1KT, Sigma-Aldrich) and incubated for 15 minutes on a rocking shaker at 4°C ...
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bioRxiv - Systems Biology 2020Quote: Aliquots of 100,000 CD14+CD16− monocytes were thawed on ice for 5 minutes then immediately resuspended in 20μL of EZ Nuclei Isolation Buffer (Sigma-Aldrich), and incubated for 5 minutes on ice ...
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bioRxiv - Genomics 2020Quote: ... nuclei were isolated by gently homogenizing tissue with a pestle in cold Nuclei Isolation Buffer from a Nuclei PURE Prep Kit (Sigma). Nuclei were pelleted and supernatant removed before being digested by incubating in 70 µl PBS ...
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bioRxiv - Cell Biology 2020Quote: ... TSA (0.6 mg/kg/day for 15 days to obtain FAPs for co-colture and for EVs isolation or 21 days for the transplant experiments; #T8552, Sigma), dissolved in DMSO solution or in DMSO alone as vehicle control (CTR) ...
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bioRxiv - Immunology 2019Quote: Isolation of mLN and splenic DCs was performed by digesting the tissue with collagenase IV (0.5 mg/mL, Sigma-Aldrich) and DNase I (12.5 μg/mL ...
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bioRxiv - Plant Biology 2020Quote: Total RNA for sRNA-seq and RNA-seq libraries was isolated using the TRI Reagent RNA isolation reagent (Sigma-Aldrich) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... coli cells that contained pL4440-CP were grown in LB supplemented with carbenicillin (100 μg/mL) followed by plasmid DNA extraction with the High Pure Plasmid Isolation Kit (Sigma). For the in vitro synthesis ...
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bioRxiv - Microbiology 2022Quote: ... Serial dilution of the sample suspensions was plated on Pseudomonas isolation agar (PIA) plates (Sigma-Aldrich, St. Louis, MO, USA) first and incubated for 1-2 days at 30 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in isotonic mitochondrial isolation buffer (250 mM sucrose, 1 mM EDTA, 20 mM Tris-HCl, pH 7.4, protease inhibitor cocktail SigmaFAST; Sigma; S8820). To isolate mitochondria ...
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bioRxiv - Cell Biology 2023Quote: ... were allowed for growth in 6-cm cell culture plates for 48 h and subjected to isolation of the cytoplasmic and nuclear fractions by using a relevant separation kit (N-3408-200ML, Sigma). During this procedure ...
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The Single-Cell Transcriptome Program of Nodule Development Cellular Lineages in Medicago truncatulabioRxiv - Plant Biology 2023Quote: ... Root segments were moved to a glass plate with 200 μL of modified Nuclei Isolation Buffer (NIB; Conde et al., 2021) containing 0.5 U/mL Protector RNase Inhibitor (Sigma Aldrich). After samples were fragmented with a sterile razor blade ...
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bioRxiv - Cell Biology 2023Quote: ... histone H1.2 knockout heterozygous (Het) and homozygous (KO) mice are isolated using the Nuclei Isolation ‘Kit Nuclei EZ Prep’ (NUC101, Sigma). Then the nuclei are treated with 500 µM MNNG with fresh-made NAD+ (N0632 ...
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bioRxiv - Genomics 2023Quote: ... Frozen cells were thawed and added to a Dounce homogenizer containing 1 mL of nuclei isolation buffer (NIB: 10 mM TrisHCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% Igepal (Millipore Sigma #18896), and 1 x protease inhibitors (Millipore Sigma #118735800001)) ...
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bioRxiv - Cell Biology 2023Quote: ... livers were perfused with HBSS containing collagenase and pronase via the portal vein prior to mechanical disruption of tissue and isolation of HSCs via an Optiprep density gradient (Sigma). Cells were cultured in high glucose DMEM enriched with L-glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... The beads were washed once with 1 mL of isolation buffer and then incubated overnight at 4°C with 10 μg of either anti-CD9 (clone CBL162, Millipore), anti-L1CAM (clone 5G3 ...
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bioRxiv - Microbiology 2023Quote: ... Bacteriophage DNA was isolated using the Phage DNA Isolation Kit (Norgen) and plasmids were isolated with the GenEluteTM Plasmid Miniprep Kit (Sigma). DNA purification from agarose gel or enzymatic reactions was performed with the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel) ...
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bioRxiv - Microbiology 2023Quote: ... and DNA was extracted from a 500 mg crushed root sample using GenEluteTM Soil DNA Isolation kit (Sigma-Aldrich, Canada) with slight modifications ...
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bioRxiv - Cell Biology 2023Quote: ... treated with LNPs containing mRNA sequences of either the anti-NSP9 nanobody or tdTomato were used for total mRNA isolation by TRI Reagent (Millipore-Sigma) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... The insoluble pellet was washed five times repeatedly by resuspension in the isolation buffer containing 0.1 g of quartz sand (Sigma-Aldrich) and vortex ...
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bioRxiv - Plant Biology 2024Quote: ... Entire leaves were fragmented on a glass plate in 200 μL of Nuclei Isolation Buffer70 containing 0.5 U/mL Protector RNase Inhibitor (Sigma Aldrich) using a razor blade ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolation of nuclear proteins was achieved with the CelLytic™ NuCLEAR™ extraction kit (Sigma Aldrich, St. Louis, MO) using the manufacturer’s recommended protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Pelleted nuclei were then washed with isolation medium and incubated in block solution consisting of 1% BSA (Sigma-Aldrich, A2153). Alexa Fluor 488 mouse anti-NeuN (1:1500 ...
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bioRxiv - Physiology 2019Quote: ... system (Millipore, Zug, Switzerland) for western blotting ...
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bioRxiv - Physiology 2019Quote: ... system (Millipore, Zug, Switzerland) for western blotting ...
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bioRxiv - Physiology 2020Quote: ... system (Millipore, Zug, Switzerland). Briefly ...
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bioRxiv - Physiology 2022Quote: ... system (Millipore, Zug, Switzerland) for western blotting ...
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bioRxiv - Microbiology 2020Quote: ... to screen larger organisms and bacterioplankton were concentrated using a tangential flow filtration system and the cells were harvested on 25 mm 0.2 m Supor filters (Millipore). The August-September seawater samples were processed using inline filtration with a 3.0 m filter (Versapor ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies bound to the cell mono-layers were detected by the addition of 3,3’,5,5’-tetramethylbenzidine liquid substrate system for membranes (Sigma-Aldrich). The cell culture infectious dose 50% (CCID50 ...
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bioRxiv - Biophysics 2024Quote: ... and then diluted in naïve HEK293T cell lysate with 1×ATP regeneration system (1 mM ATP, 25 mM creatine monophosphate (Sigma), 0.03 U/μL creatine kinase (Calbiochem) ...
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bioRxiv - Microbiology 2022Quote: TFF was performed using a TFF system (Cogent µScale TFF System, Millipore, USA). TFF was performed in two phases ...
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bioRxiv - Genomics 2019Quote: ... Debris was removed with a density gradient centrifugation using the Nuclei PURE 2M Sucrose Cushion Solution and Nuclei PURE Sucrose Cushion Buffer from Nuclei PURE Prep Isolation Kit (#NUC201-1KT, Sigma Aldrich). Nuclei PURE 2M Sucrose Cushion Solution and Nuclei PURE Sucrose Cushion Buffer were first mixed in 9:1 ratio ...
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bioRxiv - Microbiology 2019Quote: ... Ethanol was mixed with the aqueous phase and RNA isolation protocol was continued as per GenElute Mammalian Total RNA Miniprep kit (RTN350, Millipore Sigma) protocol ...
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bioRxiv - Cell Biology 2021Quote: Apical washes were harvested following incubation at 37°C with 200 µl PBS (10 min) and subsequent addition of 800 ul of the TriPure Isolation Reagent (Sigma-Aldrich) to half of apical washes ...
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bioRxiv - Cell Biology 2021Quote: RAW 264.7 cells were grown and used according to manufacturer’s instructions to obtain membrane fractions by differential sedimentation ultracentrifugation using a density gradient (Lysosome Isolation Kit, Sigma-Aldrich, LYSISO1). Briefly ...
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bioRxiv - Biochemistry 2022Quote: K562 cell line derivatives were collected by centrifugation at 1400 x g for 3 mins and nuclei were isolated using the Nuclei EZ Prep nuclei isolation kit (Sigma- Aldrich). The nuclei were fixed in 4% (v/v ...
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bioRxiv - Genomics 2020Quote: Nuclei were isolated from ~100mg of frozen frontal cortex brain tissue from Brodmann area 9 at the HIHG using the Nuclei Isolation Kit: Nuclei EZ Prep (Sigma, #NUC101). All tissues were homogenized in ice-cold EZ Lysis buffer with a glass-on-glass dounce homogenizer ...
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bioRxiv - Neuroscience 2020Quote: Total RNA was extracted from zebrafish and cultured rat brainstem and spinal cord neurons following the TRI Reagent isolation protocol (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... Bacteria were lysed by bead-beating in the presence of buffer RA-1 (NucleoSpin RNA isolation kit, Mackery-Nagel) and β-mercaptoethanol (Sigma). RNA isolation and purification was continued according to the protocol supplied by the manufacturer (Nucleospin RNA isolation kit ...
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bioRxiv - Microbiology 2019Quote: RNA isolation was carried out with 2 g of soil using RNA PowerSoil Total RNA Isolation Kit (Mo Bio Laboratories, Carlsbad, CA. USA) with phenol chloroform isoamyl alcohol 25:24:1 (Sigma-Aldrich) following the instructions of the manufacturer and resulting in 100 μL of RNA solution ...
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bioRxiv - Genomics 2019Quote: ... with 2 mL of ice-cold EZ-Prep lysis buffer from the Nuclei EZ-prep Isolation Kit (Sigma, Cat #NUC-101). The tissue was dounced 25 times with Pestle A ...
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bioRxiv - Microbiology 2019Quote: ... mini-CTX::PmexAB-lux or mini-CTX::PmexCD-lux reporter constructions integrated into their chromosome were selected on petri dishes containing Pseudomonas Isolation Agar (PIA) (SIGMA-Aldrich) with 100 μg/ml of tetracycline ...
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bioRxiv - Genomics 2019Quote: ... Individual colonies were cultured overnight before isolation of plasmid DNA using the GenElute™ miniprep kit (Sigma-Aldrich, Catalogue No. PLN350). Purified plasmids were Sanger sequenced to confirm successful cloning ...
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bioRxiv - Microbiology 2021Quote: ... aeruginosa strains were routinely refreshed from frozen stocks at -80°C and maintained on YPD (1% Bacto yeast extract, 2% Bacto peptone, 2% dextrose, 2% Bacto agar) plates or Pseudomonas isolation agar (Sigma-Aldrich) for in vitro experiments and LB agar (10 g/liter Bacto tryptone ...