Labshake search
Citations for Millipore Sigma :
201 - 250 of 10000+ citations for CTLA 4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: HEK293 or 293T cells were cultured in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich) supplemented with 10% fetal calf serum ...
-
bioRxiv - Microbiology 2022Quote: ... and HEK293 cells and were grown in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma) supplemented with 10% v/v foetal calf serum (FCS ...
-
bioRxiv - Microbiology 2023Quote: HEK293 T cells were maintained in Dulbecco’s modified Eagle medium (DMEM; Sigma, D6429) supplemented with 10% heat-inactivated FBS (Atlanta Biologicals ...
-
bioRxiv - Developmental Biology 2024Quote: HEK293 cells were seeded onto glass coverslips coated with Poly-L-Lysine (Sigma) and left to adhere overnight prior to transfection with plasmid DNA ...
-
bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
-
bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
-
Reconstitution of prospermatogonial specification in vitro from human induced pluripotent stem cellsbioRxiv - Developmental Biology 2020Quote: ... hiPSCs were plated at a density of 4-5×104 cells/cm2 onto a human fibronectin (Millipore)-coated 12 well plate in GK15 medium (GMEM [Life Technologies] with 15% KSR ...
-
bioRxiv - Microbiology 2019Quote: ... Parasites were grown in human O+ erythrocytes at 4% hematocrit in complete RPMI1640-HEPES media (Sigma-Aldrich) supplemented with 0.3% L-glutamine ...
-
bioRxiv - Cancer Biology 2020Quote: The human tongue OSCC cell lines (H357, SCC-9 and SCC-4) were obtained from Sigma Aldrich, sourced from European collection of authenticated cell culture ...
-
bioRxiv - Cancer Biology 2021Quote: ... and then incubated overnight at 4 ℃ with primary antibodies against human Kv1.3 (1: 1000, P4497, Sigma, USA), P-p38 MAPK (1 ...
-
bioRxiv - Cell Biology 2020Quote: ... We found that an additional surface treatment of 4 µg/cm2 human plasma Fibronectin (Millipore, Billerica MA) in DMEM applied overnight at 37°C with mild orbital plate agitation was necessary to create a consistent surface coating for cell adhesion ...
-
bioRxiv - Pathology 2023Quote: ... or 2 mg of human IgG isotype (n = 4) (I4506-100MG, Millipore-Sigma, Saint Louis, MO,USA) were injected subconjunctivally in the upper eyelid of eyes immediately after the post-alkali exposure irrigation ...
-
bioRxiv - Pathology 2023Quote: ... or 2 mg of human IgG isotype (n = 4) (I4506-100MG, Millipore-Sigma, Saint Louis, MO,USA) were injected subconjunctivally in the upper eyelid of eyes immediately after the post-alkali exposure irrigation ...
-
bioRxiv - Developmental Biology 2023Quote: ... Cells were sub-cultured on days 4 and 8 of differentiation onto human fibronectin (Merck Millipore, #FC010) coated plates at a ratio of 1:4 ...
-
bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
-
bioRxiv - Biophysics 2020Quote: ... The (His)8-tag was cleaved by thrombin (Sigma) using 20 U per 1 mg GltPh in the presence of 5 mM CaCl2 at room temperature overnight ...
-
bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
-
bioRxiv - Biochemistry 2020Quote: ... and quantitative immunoblotting using an anti-His antibody (Novagen).
-
bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
-
bioRxiv - Plant Biology 2019Quote: ... and anti-His (Sigma-Aldrich, St. Louis, MO, USA) antibodies.
-
bioRxiv - Biochemistry 2021Quote: ... recombinant His-Akt1 was from EMD Millipore (#14-279); recombinant GST-mTORf (containing a 30 fragment of mTOR encoding amino acids 2144-2175 ...
-
bioRxiv - Biophysics 2022Quote: ... Anti-His mAb was from Sigma (St. Louis, MO). Anti-A1 mAb CR1 was a generous gift from M ...
-
bioRxiv - Biochemistry 2023Quote: ... His tag and Flag tag (1:1000; F3165, Sigma).
-
bioRxiv - Neuroscience 2020Quote: ... HEK293 cells were seeded on to #0 coverslips coated with poly-L-lysine (Sigma) in a 24-well culture dish and kept in an incubator at 37°C under air with 5% CO2 ...
-
bioRxiv - Biochemistry 2019Quote: HEK293 cells were grown in DMEM (SLRI media facility) plus 10% FBS (Sigma, F1051). For analysis of SMN chemical antagonists ...
-
bioRxiv - Molecular Biology 2020Quote: ... Constructs were transfected into HEK293 cells with the X-tremeGENE 9 reagent (Sigma-Aldrich) in optiMEM solution for 4 hours ...
-
bioRxiv - Molecular Biology 2020Quote: ... Constructs were transfected into HEK293 cells with the X-tremeGENE 9 reagent (Sigma-Aldrich) and submitted to selection with G418 (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2019Quote: ... Blocking solution for HEK293 and iPS cells respectively contained 10% normal goat serum (Sigma) or fetal bovine serum (Eurobio ...
-
bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were grown in EX-CELL® ACF CHO Medium (Sigma-Aldrich, C5467) supplemented with 100 IU/ml penicillin and 100 μg/ml streptomycin at 37°C with 95 % humidity and 5 % CO2 ...
-
bioRxiv - Cell Biology 2022Quote: HEK293 (ATCC, CRL-1573) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Sigma, D5796) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Molecular Biology 2022Quote: HEK293 cells were maintained in DMEM supplemented with 10% fetal bovine serum (Sigma, #F0804) and 1% Penicillin/Streptoymycin (HyClone ...
-
bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: HEK293 cells stably expressing FLAG-GPR65 were plated to poly-d-lysine (Sigma Aldrich) coated 12 mm glass coverslips (Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: ... HEK293-TRPM2tet cells were treated with tetracycline hydrochloride (1 µg/ml; T7660, Sigma-Aldrich) for 48 hours ...
-
bioRxiv - Molecular Biology 2023Quote: ... HEK293 48-well cell cultures were supplemented with 100 µM guanosine (Sigma Aldrich #G6264). DMSO and 100 µM adenosine (Sigma Aldrich #A9251 ...
-
bioRxiv - Immunology 2023Quote: ... were coated overnight at 4 °C with 10 µg/ml of capture anti-human IgG (Sigma Aldrich, I2136) in PBS ...
-
bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
-
bioRxiv - Biochemistry 2023Quote: ... The cells were cultured and passaged every 2 or 3 days in Dulbecco’s modified Eagle’s medium (DMEM) (Hi Media Laboratories) supplemented with 10% FBS (Hi Media Laboratories) and 1% penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA). Both cell types were cultured at 37°C and in a humidified incubator with a 5% CO2 atmosphere ...
-
bioRxiv - Cell Biology 2020Quote: ... and Hek293 (ATCC CRL-1573, embryonic kidney) were cultured in DMEM high glucose (Sigma-Aldrich), all supplemented with 2mM glutamine (Biochrome) ...
-
bioRxiv - Biochemistry 2020Quote: ... HEK293-GNTI cells were transfected with 1 mg DNA and 3 mg PEI (Sigma-Aldrich) per liter of cells at a density of 1.3-1.8E6 cells/ml ...
-
bioRxiv - Cell Biology 2022Quote: SLC1A2/A3-expressing HEK293 Flip-In cells were plated on Poly-L-lysine hydrobromide (Sigma)-coated glass coverslips and induced with doxycycline (1 μg/ml ...
-
Evolutionary plasticity of SH3 domain binding by Nef proteins of the HIV-1/SIVcpz lentiviral lineagebioRxiv - Microbiology 2021Quote: ... HEK293 and HZ1 cells were grown in high-glucose Dulbecco’s modified Eagle’s medium (DMEM; Sigma) supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Cell Biology 2023Quote: HEK293 cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma-Aldrich, St. Louis, MO) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2023Quote: HEK293 cells (ATCC number CRL-1573) were cultured in Dulbecco’s Modified Eagle’s medium (DEME) (Sigma) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2024Quote: ... SH-SY5Y and HEK293 cells were maintained in Dulbecco’s Modified Eagles Medium (DMEM; Sigma Aldrich) with 10% and 5% FBS respectively ...
-
bioRxiv - Biochemistry 2021Quote: Human IgM purified from human serum (Sigma) was digested with trypsin ...
-
bioRxiv - Bioengineering 2019Quote: The His-TEV tag was cleaved using TEV protease (Sigma). The reaction was made overnight at 4°C into dialysis buffer for TEV (25 mM Tris-HCl ...
-
bioRxiv - Plant Biology 2019Quote: ... HIS-TAN1-GFP was conjugated with Atto488-maleimide (Sigma 28562). 4μM HIS-TAN1-GFP in 80mM PIPES ...
-
bioRxiv - Immunology 2021Quote: ... This was followed by 0.5ug/mL anti-his HRP (Sigma), and binding was detected after incubation with substrate at an absorbance of 450nm ...