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Citations for Millipore Sigma :
601 - 650 of 10000+ citations for CKLF Like MARVEL Transmembrane Domain Containing 7 CMTM7 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The peak fractions containing the MgtE TM domain protein were collected and concentrated to 10 mg/ml using an Amicon Ultra 50 K filter (Merck Millipore, USA) for crystallization.
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bioRxiv - Neuroscience 2021Quote: ... The recombinant LymCBP HAT domain (200 ng) were incubated with the histone H1 purified from calf thymus (Sigma-Aldrich, 1 μg) and 7.4 kBq of [14C]-acetyl-CoA (Moravek ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples of PARP-1 CAT domain in complex with PARP inhibitors for NMR were made by adding inhibitors (50mM in [2H6] DMSO, Sigma-Aldrich) to a final ratio of 1:1.5 (veliparib ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The kinase domain of human HASPIN (residue 452 – 798) was cloned into the expression vector pET-21a(+) (Novagen, Madison, WI, USA). The genomic DNA of HASPIN was provided from the Korea Human Gene Bank ...
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bioRxiv - Biochemistry 2023Quote: ... Full-length BTK and BTK linker kinase domain (WT and mutants) were produced by co-expressing with YopH in BL21(DE3) (Millipore Sigma) or BL21-Gold(DE3 ...
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bioRxiv - Cell Biology 2024Quote: ... A pair of gRNAs with minimum off-target and maximum on-target activity were designed and selected to specifically target sequences that encode essential protein domains of the Bend2 gene using CRISPR DESIGN TOOLS (Millipore Sigma) (gRNA1-antisense ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were incubated with TBST-Blotto containing primary monoclonal mouse anti-FLAG M2 antibody (1:2,000 dilution, Sigma) or monoclonal mouse anti-GAPDH antibody (1:2,000 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated in a primary antibody solution containing 1:250 mouse anti-NeuN (Millipore; MA, USA), 1:200 goat anti-DCX(Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2021Quote: ... Sections where incubated overnight in blocking solution containing the primary antibodies (Goat anti-ChAT 1:100, Millipore, AB144P). Sections were rinsed in PBS then incubated for 2 hours at room temperature in blocking solution containing secondary antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... and probed with antibodies in PBST buffer (PBS containing 0.05% Tween 20) with 0.5% alkali-soluble casein (Novagen) as blocking agent.
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bioRxiv - Cell Biology 2020Quote: ... Unspecific antibody binding was blocked by incubation with TBS containing 5% (w/v) bovine serum albumin (Sigma Aldrich) for one hour at RT ...
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The Batten disease protein CLN3 is important for stress granules dynamics and translational activitybioRxiv - Molecular Biology 2022Quote: ... Coverslips were then incubated with 200μl of primary antibody solution for 1 h at RT and washed 3 times with PBS before incubation with secondary antibody solution containing 0,1μg/mL DAPI solution (Sigma) for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with TSA blocking reagent containing peroxidase-conjugated sheep anti-DIG antibody (1:1,000, Sigma, 11207733910) at room temperature for 2 h ...
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bioRxiv - Molecular Biology 2021Quote: ... and then incubated with 1:300 anti-Flag primary antibody (diluted with PBS containing 5% BSA, Sigma-Aldrich) at RT for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... The primary antibody solution containing 1:400 mouse α-FLAG (clone M2, Sigma-Aldrich, St. Louis, Missouri, USA) and/or 1:1,500 rabbit α-FCaBP 64 in 1% BSA in PBS was added for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were resuspended in 800 μl IP lysis buffer containing 2.5 μl anti-FLAG M2 antibody (Sigma Aldrich) and rotated overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated in a primary antibody cocktail containing mouse anti-NeuN (Millipore, MAB277 lot 3574318, 1:500 dilution), chicken anti-tyrosine hydroxylase (TH ...
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bioRxiv - Cell Biology 2020Quote: ... containing protease (Sigma) and phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... containing rLysozyme (Millipore) and Benzonase nuclease (Millipore ...
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bioRxiv - Immunology 2022Quote: ... containing PBS (Sigma) with 2% fetal bovine serum (FCS ...
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bioRxiv - Neuroscience 2019Quote: ... containing blebbistatin (Sigma), followed by centrifugation at 300Xg for 5min to pellet the cells ...
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bioRxiv - Immunology 2023Quote: ... containing PBS (Sigma) with 2% fetal bovine serum (FCS ...
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bioRxiv - Developmental Biology 2023Quote: ... containing PMSF1mM (Sigma), and 1X Protease Inhibitor (Roche) ...
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bioRxiv - Immunology 2021Quote: ... For RBD inoculations eight-week-old male BALB/c mice were immunized with 7.5 μg of the receptor binding domain of Spike protein (kindly gifted by Andrea Gamarnik, Argentina) in 75 μL Complete Freund’s Adjuvant (CFA, Sigma, St. Louis, MO) via subcutaneous injection and boosted 2 weeks later with 5 μg of RBD in 100 μL Incomplete Freund’s Adjuvant (IFA ...
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bioRxiv - Biochemistry 2019Quote: ... the GAP-334 region of human p120GAP (residues 715-1047) and the SOScat domain of human SOS1 (residues 564-1049) are cloned into pET15b (Novagen / EMD Biosciences) with an N-terminal polyhistidine (His ...
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bioRxiv - Microbiology 2020Quote: Wild type and triple mutant human NRP1 b1b2 domain (residues 274-584) were expressed in Escherichia coli strain Rosetta-gami-2 (Novagen, Madison, WI) as a His-tag fusion in pET28b (Novagen) ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... an NPC1 domain C-targeting mAb were directly labeled with Duolink® In Situ Probemaker PLUS and Duolink® In Situ Probemaker MINUS (Sigma) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment encoding mouse C/EBPβ residues 221–296 (bZIP domain) was cloned into pET-45b(+) vector (Novagen/Merck, Darmstadt, Germay) via ‘in vivo assembly’ (35 ...
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bioRxiv - Genomics 2023Quote: ... untethered Dam and Dam-Cbx1 constructs were fused to an ERT2 domain so that the fusion protein would be translocated to the nucleus upon 4-Hydroxytamoxifen addition (4-OHT, Sigma, Cat#SML1666) (Extended Data Table 1).
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bioRxiv - Biophysics 2021Quote: ... 0.2 µM alkaline phosphatase in HEPES buffer (pH 7; Sigma-Aldrich) was added to a mica surface and visualized ...
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bioRxiv - Cell Biology 2019Quote: ... anti-HA (HA-7; 1:40,000 for WB; Cat#H9658, Sigma), rabbit anti-TPI (1:10,000 for WB ...
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bioRxiv - Immunology 2021Quote: ... Lineage-depleted cells were cultured in 10ng/ml IL-7 (Sigma), 50ng/ml Flt3-L (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... to each sample 20 µl (0.5 µg/µl) 7-dehydrocholesterol (Sigma) was added to determine extraction efficiency ...
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bioRxiv - Plant Biology 2021Quote: ... and 10 % acetic acid (Sigma Aldrich, cat No. 64-19-7)] at 4 °C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 25 μM, Sigma-Aldrich), NMDA glutamate receptor antagonist ...
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bioRxiv - Neuroscience 2021Quote: ... 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 25 μM, Sigma-Aldrich), NMDA glutamate receptor antagonist ...
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bioRxiv - Biochemistry 2020Quote: ... ampholyte high resolution pH 7–9 were purchased from Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2021Quote: ... At day 7 cells were plated to mouse laminin (#L2020, Sigma) coated well-plate formats and further differentiated until day 16 to day 21 in GDM ...
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bioRxiv - Cell Biology 2021Quote: ... 10 ng/ml OSM and 10−7 M Dex (Sigma-Aldrich). The hepatocyte maturation was proceeded for nine days as previously reported by Ishkitiev et ...
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Impairment of a distinct cancer-associated fibroblast population limits tumour growth and metastasisbioRxiv - Cancer Biology 2020Quote: ... Tumour colonies were stained 7 days later with crystal violet (Sigma). Plates were scanned using the GelCount (Oxford Optronix ...
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bioRxiv - Neuroscience 2020Quote: ... 3,4-dihydroxyphenylalanine (L-DOPA; CAS: 59-92-7, Sigma, Steinheim, Germany) was added at a concentration of 10 mg/ml ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... and stained with 200 μM 7-Azido-4-Methylcoumarin (AzMC) (Sigma). The stained cells were mounted on a glass slide and visualized using Leica TCS SP5 confocal microscope (excitation ...
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bioRxiv - Neuroscience 2022Quote: ... at 7 μg/well and 200 μl/well of Optimem (Sigma). For co-transfections ...
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bioRxiv - Synthetic Biology 2022Quote: ... and MCF-7 cells were cultured in DMEM (Sigma-Aldrich, D5796) supplemented with 10% fetal bovine serum (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The MCF-7 cells were cultured in RPMI-1640 (Sigma, USA) while MCF-10a cells were maintained in Ham’s F-12 (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μM hydrocortisone and 10-7 M of dexamethasone (Sigma Aldrich).
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bioRxiv - Developmental Biology 2021Quote: ... The sample was stained with 7-AAD (2ug/uL, Sigma-Aldrich) in order to visualize nuclei during FACS sorting ...
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bioRxiv - Immunology 2020Quote: ... then cultured for 7 days in complete media (RPMI 1640 (Sigma) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... or the ACE2 product Ang (1-7) (Sigma, #A9202; 1 μM) in the same culture conditions described above.
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bioRxiv - Biochemistry 2019Quote: 2 µl of 1 mM 2’,7’ Dichlorofluorescein diacetate (Sigma-Aldrich) dissolved in DMSO was added to 198 µl of cell culture in a black f-bottom 96 well plate (Greiner bio-one ...