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Citations for Millipore Sigma :
351 - 400 of 777 citations for CD3d Cynomolgus HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 10% heat-inactivated FCS and 1 % Penicillin/Streptomycin (Sigma cat#P0781) in a 28°C incubator with 5 % CO2 to 80-90 % confluency before harvesting ...
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bioRxiv - Neuroscience 2021Quote: ... eyes were incubated in 20% foetal bovine serum (FCS) (F7524; Sigma) in order to block proteinase K activity ...
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bioRxiv - Biochemistry 2020Quote: ... goat-anti-mIgG(Fc) conjugated with HRP (1:42000, A0168, Sigma) for mFc tagged antigen versions or goat-anti-hIgG(Fc ...
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bioRxiv - Cancer Biology 2021Quote: ... fluorinated oil (Fluorinert FC-40, Cat# 86508-42-1, Sigma-Aldrich) was loaded into the device for sealing microwell arrays after introducing freeze-thaw lysis buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 1% FCS and with 0.1% Penicillin Streptomycin (PS, Sigma), minced and incubated at 37°C with the digestion solution (collagenase 2.5mg/ml in EBSS) ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated anti-human Fc antibody (1:10000 dilution, Sigma Aldrich) was used ...
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bioRxiv - Microbiology 2020Quote: ... streptomycin (100 mg/ml) and 5% fetal calf serum (FCS) (Sigma). IBDV infections were performed on preconfluent (ca ...
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bioRxiv - Immunology 2020Quote: ... supplemented with 2% (v/v) FCS and 2mM EDTA (Sigma, E7889). Wash buffer consists of PBS supplemented with 2% FCS.
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bioRxiv - Immunology 2021Quote: ... either supplemented with 10% HI dialysed and filtered FCS (Sigma-Aldrich) 0.1M HEPES ...
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bioRxiv - Biophysics 2022Quote: ... containing 10mM HEPES (Roth) and 0.5% FCS (Biochrom GmbH, Merck Millipore). One chamber slide was used for gel expansion and the other one as non-expanded control ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in PBS supplemented with 1% FCS (S7524, Sigma) and 2mM EDTA and kept at 4°C at any time ...
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bioRxiv - Bioengineering 2023Quote: ... 100μL of ALP-conjugated anti-human IgG Fc-ALP (Sigma-Aldrich) diluted 1:5000 in M-PBST was added and incubating for 1 hour ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Media were supplemented with 5% fetal calf serum (FCS) (Sigma-Aldrich) and 1% penicillin/streptomycin (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with RPMI containing 10% (v/v) FCS (Sigma) and 2 mM L-Glutamine (R10) ...
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bioRxiv - Immunology 2023Quote: ... and 10% (v/v) heat-inactivated fetal calf serum (FCS) (Sigma). Cells were added to sterile 96 well U-bottom tissue culture plates pre-coated with purified NA/LE anti-CD3 (Clone 145-2C11 ...
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bioRxiv - Immunology 2023Quote: ... Anti-Mouse IgG (Fc Specific)-Alkaline Phosphatase antibody (Sigma-Aldrich #A2429), at a 1:4000 dilution was added ...
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bioRxiv - Plant Biology 2023Quote: ... 10 µM ABA or 1 µM Fusicoccin (FC) (all from Sigma) dissolved in absolute ethanol ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 20% fetal calf serum (FCS; Sigma-Aldrich cat# F7524) and 50 ng/mL vascular endothelial growth factor (VEGF165 ...
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bioRxiv - Cell Biology 2024Quote: ... was used to prepare unstimulated cells or FCS (Biosera or Sigma) to prepare stimulated cells ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant ephrins or Fc were preclustered in a 5:1 molar ratio with either mouse or goat anti-human Fc (Millipore Sigma cat. no. 16760 and 12136 respectively) for 30’ at 37°C.
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bioRxiv - Biophysics 2021Quote: The cAMP synthesis of heterologously expressed sAC in HEK293 lysates was induced by applying di-sodium-ATP (Sigma Aldrich) and MgCl2 or MnCl2 in isotonic HEPES buffer ...
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bioRxiv - Biochemistry 2020Quote: ... control and SENP3 over-expressed HEK293 cells were stimulated by poly (I:C) for 6h and lysed in NETN buffer supplemented with 10mM PMSF (Sigma). The whole cell lysates were immunoprecipitated with 10 μl Anti-Flag (M2)-agarose (Sigma ...
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bioRxiv - Microbiology 2020Quote: The human embryonic kidney cell lines HEK293 and HEK293T were cultured in Dulbecco’s modified Eagle’s medium (D1152, Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells transfected and grown overnight prior to incubation with 5μg/ml cycloheximide (CHX; Sigma-Aldrich, St. Louis, MO) in the presence and absence of 15μM CQ (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... murine fibroblast (3T3, laboratory stocks) and HEK293 (laboratory stocks) cell lines were maintained in Dulbecco’s minimal essential medium (DMEM, Sigma) supplemented with 10% (v/v ...
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Splicing variation of BMP2K balances endocytosis, COPII trafficking and autophagy in erythroid cellsbioRxiv - Cell Biology 2020Quote: HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) and K562 cells in RPMI-1640 medium (Sigma-Aldrich). All media had high glucose concentration and were supplemented with 10% fetal bovine serum and 2 mM L-glutamine (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2023Quote: ... HEK293 cells were then transduced overnight with the filtered virus in the presence of 8 µg/mL polybrene (Millipore); the amount of virus used was optimized to ensure the infection rate of ∼20% ...
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bioRxiv - Neuroscience 2023Quote: ... were produced in HEK293 cells by mixing lentiviral vector and packaging vectors using branched polyethyleneimine (bPEI) (MW 25Kda, Sigma) as the transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: U2OS osteosarcoma cells and HEK293 (human embryonic kidney) cells were grown in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma, D1152) supplemented with 10% fetal bovine serum (FCS ...
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bioRxiv - Cell Biology 2021Quote: ... 4°C) and the cell pellets washed twice with 10% FCS in PBS and resuspend in Dapi solution (Sigma; 50μg/ml in 10% FCS in PBS; 100μl/well) and stored at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 10% fetal calf serum or FCS (Sigma-Aldrich, St. Louis, MO, USA) as well as 1% antibiotics (penicillin and streptomycin) ...
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bioRxiv - Cancer Biology 2019Quote: ... with 10% Fetal Calf Serum (FCS) (Sigma-Aldrich, St Louis, MO, USA) and 2 mM L-Glutamine (BE17-605F ...
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bioRxiv - Molecular Biology 2019Quote: ... Secreted knottin-Fc fusion proteins were purified using Protein A Sepharose (Sigma) followed by size exclusion chromatography (Superdex 75 column ...
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bioRxiv - Cancer Biology 2020Quote: ... All media were supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich) and all cells were kept at 37°C with 5% CO2 ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... containing 7 % fetal calf serum (FCS) (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), 1% GlutaMAX (Gibco) ...
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bioRxiv - Cell Biology 2021Quote: ... + 10% fetal calf serum (FCS; Sigma Aldrich, St. Louis, Missouri, United States) on plastic or in PRIME-XV MSC Expansion XSFM medium (FUJIFILM Irvine Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... 10% FCS and supplemented when indicated with 1 μM thapsigargin (TG, Sigma).
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bioRxiv - Cancer Biology 2021Quote: ... Digested tissue was re-suspended in 10% FCS (Capricorn) DMEM (Sigma Aldrich), Pen/Strep (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were blocked in 1 % BSA and 4% FCS (Sigma-Aldrich) buffer and incubated for 30 min with primary and secondary antibodies (listed above ...
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bioRxiv - Cell Biology 2020Quote: ... Chemotaxis experiments towards 2.5% FCS and 100 ng/ml HGF (#H9661, Sigma) in DMEM were performed with μ-slide chemotaxis chambers (Ibidi ...
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bioRxiv - Biochemistry 2022Quote: ... horseradish peroxidase (HRP)-conjugated anti-human IgG Fc specific antibody (Sigma, USA) was added at the dilution of 1:2,000 and incubated at 37 °C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... with goat anti-human Fc-specific IgG (Sigma-Aldrich – Saint Louis, MO) immobilized with a target capture level of ~70 RU ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10% heat inactivated foetal calf serum (FCS; Sigma Aldrich, Germany), 100 U/ml penicillin and 100U/ml streptomycin ...
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bioRxiv - Microbiology 2022Quote: ... an overlay of DMEM containing 2% FCS and 0.6% Methylcellulose (Sigma-Aldrich) was added to the cells ...
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bioRxiv - Molecular Biology 2019Quote: ... supplemented with 10 % foetal calf serum (FCS; Sigma - catalogue no. F-7524), 1,000 units/ml LIF ...
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bioRxiv - Immunology 2020Quote: ... The pellet was resuspended in IMDM containing 2% FCS and formaldehyde (Sigma) was added to a final concentration of 1% ...
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bioRxiv - Pathology 2020Quote: ... 5-azacytidine (5-aza, Sigma-Aldrich; 1 µM in 10% FCS DMEM) for 7 days according to previous publications12 ...
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bioRxiv - Immunology 2019Quote: ... The pellet was resuspended in IMDM containing 2% FCS and formaldehyde (Sigma) was added to a final concentration of 1% ...
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bioRxiv - Immunology 2019Quote: ... supplemented with 10% (v/v) heat-inactivated foetal calf serum (FCS) (Sigma), 2mM (1% v/v ...
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bioRxiv - Immunology 2021Quote: ... with 10% FCS and 2 mM L-Glutamine (Sigma, Cat No. G7513) and 1% penicillin/streptomycin (Invitrogen cat no ...