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Citations for Millipore Sigma :
501 - 550 of 3552 citations for CD24 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... human fibronectin (Sigma-Aldrich) 5 µg/ml ...
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bioRxiv - Cell Biology 2021Quote: ... human serum (H2918, Sigma), DMXAA (D5817 ...
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bioRxiv - Cancer Biology 2022Quote: ... and human IgG (Sigma), respectively ...
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bioRxiv - Molecular Biology 2022Quote: ... human insulin (I9278, Sigma), human TGFβ1 (PHP143B ...
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bioRxiv - Cell Biology 2022Quote: ... or human fibronectin (Sigma). We found that BSA and collagen IV coating both resulted in reliable cell adhesion ...
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bioRxiv - Immunology 2022Quote: ... Pooled human serum (Sigma) dilutions and pooled saliva were prepared in a 96-well plate ...
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bioRxiv - Microbiology 2020Quote: ... 5% human serum (Sigma), Penicillin-Streptomycin ...
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bioRxiv - Microbiology 2022Quote: Human fibrinogen (Millipore Sigma) was resuspended in PBS at 1 ng/μL ...
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bioRxiv - Immunology 2022Quote: ... + 10% human serum (Sigma) + 2mM L-glutamine (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Human fibrinogen (Sigma-Aldrich) labeled with fluorescent Alexa Fluor 647 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 5% human serum (Sigma) and 50% virus solution in RPMI media (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... Human AB serum (Sigma), recombinant IL-2 ...
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bioRxiv - Cell Biology 2022Quote: ... with human fibronectin (Sigma) 10 μg/ml for 2h at room temperature under a laminar flow hood ...
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bioRxiv - Cell Biology 2023Quote: ... human serum (H2918, Sigma), Zymosan (Z4250 ...
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bioRxiv - Bioengineering 2023Quote: ... human recombinant LIF (Millipore), and Heparin (StemCell Technologies) ...
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bioRxiv - Biochemistry 2022Quote: ... human serum (Sigma-Aldrich), trypsin (Promega) ...
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bioRxiv - Immunology 2023Quote: ... Human interferon-gamma (Sigma) was reconstituted in sterile water and used at 20 ng/ml ...
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bioRxiv - Bioengineering 2024Quote: ... human laminin (Sigma-Aldrich) and polyethylene glycol diacrylate (PEGDA ...
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bioRxiv - Cell Biology 2024Quote: ... Human AB serum (Sigma) was used to prepare unstimulated cells or FCS (Biosera or Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant insulin human (≥ 98%), somatostatin-14 (≥ 97%, human HPLC grade), and urocortin-3 (≥ 97%, human HPLC grade) were all purchased from Sigma-Aldrich. Taxonomy will only be indicated for insulin ...
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bioRxiv - Biophysics 2021Quote: ... The plasmids were incorporated into pcDNA3.1(+) vectors and were transiently transfected into HEK 293T cells using PEI (Milipore Sigma) as previously described 66 ...
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bioRxiv - Cell Biology 2021Quote: ... HEK-293T cells were transfected with gene of interest for 24 hrs and treated with CHX (100μg/ml; Sigma). Cell lysates were prepared at indicated time points and subjected to 10% SDS-PAGE followed by western analysis as described above.
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bioRxiv - Cell Biology 2021Quote: ... and HEK 293T cells were transfected with a 1:3 ratio of DNA:polyethylenimine (PEI, 25 kDa linear, Sigma-Aldrich). Transfection of siRNA was performed using X-tremeGENE siRNA Transfection Reagent (Roche ...
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bioRxiv - Biophysics 2022Quote: ... The plasmids were incorporated into pcDNA3.1(+) vectors and were transiently transfected into HEK 293T cells using PEI (Milipore Sigma) as previously described 32 ...
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bioRxiv - Microbiology 2022Quote: ... HEK 293T-hACE2-TMPRSS2 cells (called herein HEK-ACE2-TMPRSS2) were induced for TPMRSS2 expression by addition of doxycycline (0.5 μg/mL, Sigma) and were maintained in DMEMc with blasticidin (10 μg/mL ...
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bioRxiv - Microbiology 2021Quote: The Env expression plasmids were used to transfect HEK-293T cells with X-tremeGENE HP DNA Transfection Reagent (Sigma) in combination with either a Tat expression plasmid pTat for Env expression and fusion assays ...
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bioRxiv - Molecular Biology 2021Quote: The lysates of HEK-293T cells transfected with circPTPN12 plasmid were incubated with Protein-A/G agarose beads (Millipore) and antibody against Ago2 (Abcam ...
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bioRxiv - Microbiology 2020Quote: ... Human embryonic kidney (HEK) 293T cells and chicken DF-1 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Sigma) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2020Quote: ... human embryonic kidney (HEK) 293T cells and chicken DF-1 cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM; Sigma) supplemented with 10% FBS and 100 U/ml Penicillin-Streptomycin (complete DMEM) ...
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bioRxiv - Biochemistry 2022Quote: Expression of recombinant extracellular proteins was performed by transient transfection in HEK−293F cells using polyethylenimine (PEI, Sigma Aldrich). 300 mL of culture at a density of 1.0 × 106cells/mL was transfected with 300 μg DNA using 450 μg PEI ...
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bioRxiv - Biophysics 2022Quote: Human embryonic kidney cell line (HEK-293T) were seeded on 25 mm No.1.5 H cover glass (Marienfeld) coated with poly-L-lysine (Sigma). 0.15 million cells were maintained in 2ml Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... and human embryonic kidney (HEK) 293T cells authenticated by RIKEN were cultured in Dulbecco’s modified Eagle’s medium (DMEM; D6546; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Bioengineering 2023Quote: ... human iPSC-derived cardiomyocytes were suspended in fibrinogen from human plasma (Sigma-Aldrich) at a concentration of 50x106 cells/ml ...
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bioRxiv - Biochemistry 2021Quote: ... ACER3-wt or ACER3-E33G expression was induced in FreeStyle 293-F(TetR) cells with 1 µg/mL doxycycline (Sigma). Induced cells were counted and resuspended at 0.5×106 cells/mL in medium containing vehicle (0.0125% DMSO ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were produced by transfection of 293-6E cells (National Research Council Canada) using branched polyethylenimine (PEI) 25kDa (Sigma-Aldrich). Cells were maintained at 37°C and 6% CO2 FreeStyle 293 Expression Medium (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... and retinal levels of human Pi and serum levels of human insulin were measured using human Pi and human insulin ELISA kits (EZHPI-15K and EZHI-14K, respectively; Millipore, Darmstadt, Germany) according to the manufacturer’s instructions and as described previously (Isiegas et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... Blocking was carried out in freshly made blocking buffer (5% FCS, Sigma-Aldrich, Gillingham ...
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bioRxiv - Immunology 2022Quote: ... in 10 ml VLE DMEM (Biochrom) containing 10% heat-inactivated FCS (Sigma-Aldrich), 0.1% 2-mercaptoethanol (Thermo Fischer Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Spleens were gently meshed in FACs buffer (PBS containing 3% FCS from Sigma) using a 40μM cell strainer to prepare single cell suspensions ...
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bioRxiv - Molecular Biology 2019Quote: ... MDCKII cells were grown in DMEM + 10%FCS + Antibiotic/Antimycotic solution (Sigma A5955)(Williams et al. ...
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bioRxiv - Genomics 2021Quote: ... supplemented with 10% FCS tested to be dox-free (Sigma-Aldrich, Taufkirchen, Germany) and penicillin and streptomycin (final concentrations 100 units/ml and 100µg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% (w/v) fetal calf serum (FCS) (Merck Millipore, Berlin, Germany) and 1% (w/v ...
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bioRxiv - Plant Biology 2022Quote: ... and anti-Mouse IgG (Fc-specific)-Peroxydase (Sigma, dilution: 1/10000, 1.5hr incubation). Anti-Hsp90 (Agrisera ...
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bioRxiv - Immunology 2020Quote: ... the media was replaced by FCS-free medium containing 0.1% BSA (Sigma-Aldrich) and cells were stimulated with 100 μl milk EVs or EV-depleted controls in the presence or absence of 5 μg/ml Poly I:C (Invivogen) ...
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bioRxiv - Microbiology 2021Quote: ... the Fc tag of all APN orthologs was removed using thrombin (Millipore Sigma) in a reaction mixture containing ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% fetal calf serum (FCS, Sigma-Aldrich, St. Louis, MO, USA), 10mM HEPES ...
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bioRxiv - Developmental Biology 2021Quote: ... We injected in each matrix 25 μg of Recombinant Sema3C-Fc (Sigma-Aldrich) in a concentration of 50 μg/ml in PBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... cell media was supplemented with 500 μg/mL 5-FC (Sigma, MO, USA). For control conditions ...
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bioRxiv - Immunology 2019Quote: ... before cryopreservation in freezing medium (10% DMSO [Sigma-Aldrich], 90% FCS [Sigma-Aldrich]). This method preserves immune cell viability ...