Labshake search
Citations for Millipore Sigma :
51 - 100 of 6269 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: The following antibodies were purchased: CNTNAP2 (C-terminal, rabbit polyclonal, Millipore, Darmstadt, Germany), CNTNAP2 (N-terminal ...
-
bioRxiv - Cell Biology 2024Quote: ... 1 uM Calpain Inhibitor Peptide (Sigma-Aldrich C-9181;); BSA was included to prevent non-specific binding ...
-
bioRxiv - Cell Biology 2022Quote: ... followed by a AVPVDSRGSRA linker and a C-terminal 3X FLAG sequence (Sigma-Aldrich). All mutations in Arl3 were created using PCR mutagenesis (Weiner et al. ...
-
bioRxiv - Plant Biology 2022Quote: ... anti-CRISPR/Cas9 C-terminal mouse monoclonal antibody (SAB4200751; Sigma-Aldrich, St. Louis, MO) and ProSignal Dura ECL Reagent (Genesee Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... and a C-terminal His6-tag was cloned into the expression vector pET21b (Novagen). The MALT1(PCASP-Ig3)339–719-his construct was transformed into Escherichia coli strain T7 express competent cells ...
-
bioRxiv - Cell Biology 2023Quote: ... The primary antibodies used were anti-LaminA (C-terminal; 1:1000; L1293; Sigma-Aldrich), anti-SUN1 (1:500 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Serum insulin and c-peptide levels were measured by insulin or C-peptide ELISA respectively following the manufacture’s protocol (Sigma Aldrich, St. Louis, MO, USA). Mouse metabolic testing and fasting methods followed established protocols [16,33,34].
-
bioRxiv - Cell Biology 2019Quote: ... Human c- peptide ELISA kit (cat # EZHCP-20K, Millipore MA), Human Insulin ELISA kit (cat # ELH- Insulin ...
-
bioRxiv - Neuroscience 2023Quote: ... Tau ladder (six human tau recombinant isoforms, Sigma-Merck) was used to identify tau isoforms in NCI-H716 cells ...
-
bioRxiv - Molecular Biology 2020Quote: ... was replaced by the wild-type CARD14 gene obtained from pFN21AE3344 with N-terminal 3xFLAG tag peptides (DYKDHDGDYKDHDIDYKDDDDK) (Sigma-Aldrich). To generate CARD14-expressing vector (pTRE3G-Pur-3FLAG-CARD14wt ...
-
bioRxiv - Physiology 2020Quote: ... Mouse insulin and C-peptide were detected by multiplex ELISA (Millipore) kit in the plasma samples collected during the oral glucose challenge ...
-
bioRxiv - Cell Biology 2021Quote: ... anti-ADAM10 rabbit polyclonal and anti-APP C-terminal rabbit polyclonal antibodies were from Sigma-Aldrich Company Ltd ...
-
bioRxiv - Cell Biology 2020Quote: The C-terminal tails of Taxol-stabilized Cy5 microtubules were cleaved using Subtilisin A (Sigma Aldrich) as previously described (72) ...
-
bioRxiv - Neuroscience 2022Quote: ... followed by western blotting using rabbit antibody against the C-terminal part of TMEM150c (ABN2266, Millipore), mouse β-actin (A1978 ...
-
bioRxiv - Microbiology 2022Quote: ... The blot was probed with a-C-terminal-DHFR rabbit antibody (1 :1000 Sigma-Aldrich, D0942) and goat anti-rabbit IgG HRP-conjugated secondary antibody (1:10,000 ...
-
bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
-
bioRxiv - Cell Biology 2024Quote: ... Pellet and supernatant were analysed by western blot using a C-terminal TDP-43 antibody (Sigma).
-
bioRxiv - Microbiology 2024Quote: ... ISG65 and ISG65ΔL2 were biotinylated on their C-terminal AviTag using the Enzymatic Biotinylated Kit (Sigma).
-
bioRxiv - Molecular Biology 2020Quote: ... followed by elution with 100 μg/ml c-Myc peptide (Sigma-Aldrich) on an end-to-end rocker at room temperature for 1 hr ...
-
bioRxiv - Molecular Biology 2021Quote: ... overnight at 4°C and eluted with the HA peptide (Sigma #I2149) overnight at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... Primary antibody directed against the C-terminal part of APP (APP-C-ter) (A8717) was purchased from Sigma-Aldrich (St-Louis, MO, USA). Anti-PS1 (D39D1 ...
-
bioRxiv - Neuroscience 2021Quote: ... which has the hSyn promoter followed by Flpo with 3×FLAG C-terminal tag (OGS629, Sigma-Aldrich). This plasmid was verified by sequencing and used throughout the study ...
-
bioRxiv - Molecular Biology 2023Quote: ... The T7 expression vector pET21b with C-terminal 6xHis tag was originally purchased from Novagen (NdeI-XhoI). 5hmdCTP/dGTP/dATP/dTTP mix was purchased from Zymo Research ...
-
bioRxiv - Microbiology 2023Quote: ... The T7 expression vector pET21b with C-terminal 6xHis tag was originally purchased from Novagen (NdeI-XhoI). E ...
-
bioRxiv - Cell Biology 2019Quote: ... and rat/mouse c-peptide ELISA kit (cat # EZRMCP 2-21K, Millipore MA).
-
bioRxiv - Immunology 2020Quote: ... 1.1 µmol of each HIV synthetic peptide (with C-terminal cysteine added) and 0.22 milliliters 0.3% glutaraldehyde solution (ACS reagent grade, pH 5.5, Sigma-Aldrich) at RT were slowly mixed and left to stand for 1.50 hrs ...
-
bioRxiv - Immunology 2021Quote: ... 1.1 µmol of each HIV synthetic peptide (with C-terminal cysteine added) and 0.22 milliliters 0.3% glutaraldehyde solution (ACS reagent grade, pH 5.5, Sigma-Aldrich) at RT were slowly mixed and left to stand for 1.50 hrs ...
-
bioRxiv - Biochemistry 2021Quote: A C-terminal 10x His-tagged Synechocystis PCC 6803 ocp gene (slr1963) was cloned in a pCDFDuet (Novagen), resulting in a plasmid named pEP4 ...
-
bioRxiv - Molecular Biology 2022Quote: ... the Golgi apparatus was detected with rabbit monoclonal anti-GM130 (C-terminal, G7295, SIGMA-Aldrich, Prague, Czech Republic). Detection of bound primary antibodies was achieved using donkey anti-mouse IgG Alexa Fluor 488 donkey anti-rabbit IgG Alexa Fluor 555 secondary antibodies (Thermo Fischer Scientific) ...
-
bioRxiv - Plant Biology 2020Quote: ... β-Glucuronidase (N-Terminal (Sigma-G5420), GFP (Roche-11814460001) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and splice isoform specific PCRs were performed (Sigma Aldrich High fidelity Taq) using appropriate primers (Supplemental table 8 ...
-
bioRxiv - Neuroscience 2023Quote: ... 5 ng/isoform per lane for Tau ladder (Sigma, cat. no. T7951), 8 µg per lane for mouse brain extracts ...
-
bioRxiv - Molecular Biology 2020Quote: ... C-18 ZipTip columns for concentrating and desalting the peptides were purchased from Millipore, Billerica ...
-
bioRxiv - Cell Biology 2019Quote: ... the nanobody containing an extra C-terminal cysteine was reduced with 5 mM TCEP (Sigma-Aldrich, Cat. No. C4706) for 2 h on ice ...
-
bioRxiv - Microbiology 2019Quote: ... and C-terminal 222-bp of the dosR ORF) were cloned in T7-lac-based expression system pET28b (Novagen) as recombinant fusion proteins containing an N-terminal His6-tag ...
-
bioRxiv - Biochemistry 2021Quote: ... The C-terminal domain (CTD) of DRH-3 (residues 940-1108) was cloned into the expression vector pET15b (Novagen), and the resulting clone carries an N-terminal His6 tag and a thrombin cleavage site ...
-
bioRxiv - Biochemistry 2022Quote: ... CLP1 contained a C-terminal FLAG-Tag and the sample was purified using ANTI-FLAG M2 Affinity Gel (Sigma), eluted with Pierce 3X FLAG peptide (Thermo ...
-
bioRxiv - Microbiology 2022Quote: ... Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3) cells (Novagen). Protein expression was induced during logarithmic growth through the addition of 0.5mM isopropyl-thiogalactopyranoside (IPTG ...
-
bioRxiv - Neuroscience 2022Quote: ... mouse anti-β−III tubulin isoform antibody (1:500, Millipore, cat#MAB1637, RRID:AB_2210524), for 2 hours at RT and followed by a goat anti-mouse Alexa Fluor 488 conjugated secondary antibody (1:500 ...
-
bioRxiv - Biochemistry 2021Quote: Purified CA from bovine erythrocytes (containing CAII isoform) was obtained from Sigma (USA) Cat ...
-
bioRxiv - Microbiology 2019Quote: ... DNA encoding the POTRA domains (1-4) of BamA with a C-terminal strep-tag (but without the signal sequence) was cloned in pET21a (Novagen). All the proteins above were overexpressed in BL21(DE3 ...
-
bioRxiv - Molecular Biology 2021Quote: ... whereas the C-terminal flanking sequence is removed by the treatment with HRV-3C protease (Millipore Sigma, St. Louis, MO) in-between two rounds of Ni-NTA affinity chromatography.
-
bioRxiv - Neuroscience 2021Quote: Rat recombinant RIIβ was derived as a C-terminal 6XHis fusion by Ni-NTA affinity purification using standard pET vector methods (Novagen). RIIβ was phosphorylated in vitro by Cdk5 or PKA using standard conditions (Bibb et al. ...
-
bioRxiv - Molecular Biology 2019Quote: ... Mouse CTCF cDNA corresponding to the full-length 736 amino acids was cloned with C-terminal 6xHis tag into pFLAG-2 (Sigma). FLAG-CTCF-6xHis protein was induced in Rosseta-Gami B-cells (EMD Millipore ...
-
bioRxiv - Biochemistry 2020Quote: ... whereas the C-terminal flanking sequence is removed by the treatment with HRV-3C protease (Millipore Sigma, St. Louis, MO). For crystallization ...
-
bioRxiv - Microbiology 2019Quote: ... This fragment was cloned by ligation independent cloning into the linearized pIEX-10 EK-LIC vector which carries a C-terminal 10xHis tag (Novagen) to generate pIEX-10 ...
-
bioRxiv - Microbiology 2019Quote: ... was cloned into the protein expression vector pET28a (+) containing an IPTG-inducible promoter and a C-terminal 6x-His tag (Novagen). Expression of CosR-His was then induced in E ...
-
bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
-
bioRxiv - Developmental Biology 2023Quote: ... His-tagged recombinant proteins of HSF5 N-terminal region (aa1-209) and HSF5 C-terminal region (aa324-624) were produced by inserting cDNA fragments in-frame with pET19b (Novagen) respectively in E ...
-
bioRxiv - Biochemistry 2023Quote: ... the Streptag-purified protein after removal of the C-terminal affinity-GFP-tag was collected and concentrated using 100 kDa MW cut-off spin concentrator (Amicon Merck-Millipore) further purified by size-exclusion chromatography (SEC) ...