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Citations for Millipore Sigma :
251 - 300 of 300 citations for Brucella Abortus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... 5 μm tissue sections were subject to antigen retrieval (0.01 M citric acid and 0.1% Triton X) (Sigma, St. Louis, MO), blocked with the avidin/biotin blocking kit (Vector Laboratories) ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections were subjected to a heat-induced antigen retrieval step prior to exposure to primary OSM antibody (1:50, HPA029814, Sigma-Aldrich). Immunodetection was performed using the Roche Ventana BenchMark ULTRA IHC/ISH staining system ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 8 and the α-NOCT antibody was antigen affinity purified with NOCT (64-431) immobilized on Immobilon-P PVDF membrane (Millipore). Guinea pig α-NOCT was eluted from the membrane with 0.1 M glycine and 20% glycerol ...
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bioRxiv - Genetics 2022Quote: Longitudinal paraffin-embedded sections were processed as described (Gomez et al., 2022) following 1-hour antigen retrieval with 2mg/ml Hyaluronidase (Sigma-Aldrich) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (clone PAb416, Sigma Aldrich - Saint-Quentin-Fallavier France) diluted 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... rat anti-PDGFRβ (eBiosciences 14-1402-81), rabbit anti-AQP3 (Alomone Labs, AQP-003, after antigen retrieval) rabbit anti-Runx1 (Sigma, HPA004176), rabbit anti-IRF9 (Cell Signalling ...
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bioRxiv - Microbiology 2023Quote: ... at 15 days and 36 days with 1:1 (v/v) mixture of antigen with incomplete Freund’s adjuvant (Sigma-Aldrich, USA). After the first immunization ...
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bioRxiv - Genetics 2023Quote: ... Antigen retrieval was performed by pressure cooker incubation of slides in 1L of pre-warmed 1X Citrate Buffer (Sigma Aldrich #C9999) + 0.1% Tween in dH2O for a total of 15 mins (human ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by antigen retrieval using Tris EDTA buffer pH 9 (Genemed, 10-0046) or 1x sodium citrate at pH 6 (Sigma, C9999) according to manufacturer’s recommendation for specific antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were immediately subjected to heat-mediated antigen retrieval at 95°C for 10 minutes in 1X pH6.0 sodium citrate buffer (Sigma-Aldrich, #21545) using Micromed T/T Mega Multifunctional Microwave Rapid Histoprocessor (Milestone) ...
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bioRxiv - Neuroscience 2023Quote: ... coverslips were first incubated with Tris-glycine solution 5min at room temperature followed by blocking of non-specific antigens with a cold-water fish blocking buffer (Sigma-Aldrich) for 30min ...
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bioRxiv - Immunology 2023Quote: Eosinophilic airway inflammation was induced by two intraperitoneal injections of 5μg of cockroach antigen (CA, Greer Laboratory, Lenoir, NC) mixed in 100μl of alum adjuvant (aluminum hydroxide, Sigma, St. Louis, MO) at day 0 and 7 ...
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bioRxiv - Neuroscience 2021Quote: ... tissues underwent antigen retrieval by incubating for 15 minutes in hot 10mM sodium citrate buffer pH=6.0 (Sigma cat. no. S-4641). Sections were rinsed in PBS and incubated in 3% H2O2/PBS for 15 minutes ...
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bioRxiv - Immunology 2019Quote: ... sections from the initial portions of sockets were incubated with primary antibody for Proliferating Cell Nuclear Antigen (PCNA) (#MAB424, Merck Millipore, Darmstadt, Germany) diluted to 1:150 in blocking solution and incubated at 4°C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Antigen retrieval was performed using 1X Sodium Citrate Buffer diluted from a 10X stock (10X Stock: 100mM trisodium citrate (Sigma, Cat#S1804), 0.5% Tween 20 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... Gels were blotted onto nitrocellulose membranes and probed for each antigen using the following antibodies at the indicated dilutions: Human PEAK1 (Millipore 1:400), Mouse PEAK1 (Abgent 1:400) ...
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bioRxiv - Immunology 2020Quote: ... Detection of GFP and Iba1 required high-temperature antigen unmasking in 0.01 M citrate buffer (pH 6.0) (Sigma-Aldrich, San Diego, CA). Sections were treated with biotin and streptavidin blocking solutions (Vector Laboratories ...
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bioRxiv - Neuroscience 2019Quote: ... pre-incubation of the neurotransmitter antibodies used in this study with their corresponding antigen (100 μM - 400 μM; GABA (Sigma-Aldrich, A2129), glutamate (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... whilst H antigen was detected with 1 μg/ml Ulex Europaeus Agglutinin I (UEA-I) lectin labeled with fluorescein isothiocyanate (Sigma-Aldrich, Germany). Lea and Lex were detected with 0.5 μg/ml of mAbs MEM-158 and 7Le (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... The polyreactivity of hybridoma-obtained IgA was tested by adding them to ELISA plates that were coated with common antigens dissolved in PBS before coating ELISA plates: Lipopolysaccharide (Sigma-Aldrich; L4391), double stranded DNA (Sigma-Aldrich ...
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bioRxiv - Pathology 2022Quote: ... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
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bioRxiv - Immunology 2022Quote: ... The moANCA518 binding to the antigen was detected after incubation for 1 hour at RT of alkaline phosphatase conjugated goat anti-human IgG (Sigma, A-9544) antibody in a 1:10,000 dilution in TBS (20 mM Tris ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to S1 and RBD antigen was detected using a three-step approach consisting of anti-mouse or antirat Fc biotin (Sigma Aldrich B7401) followed by streptavidin-HRPO (Life Technologies cat# SA1007 ...
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bioRxiv - Microbiology 2023Quote: Proteins in the cell and viral lysates (5 μg of total protein and 10 ng of p24 antigen) were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Cat# IPVH00010). Membranes were blocked with 5% milk in PBS containing 0.1% Tween 20 (0.1% PBST ...
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bioRxiv - Immunology 2023Quote: ... The first kit used was the Milliplex® SARS-CoV-2 antigen panel 1 IgA assay (Millipore Sigma, Cat No. HC19SERA1-85K) with the Wuhan-1 strain RBD and N proteins included ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were screened using ELISA (see below) and antigen-reactive clones were expanded in RPMI-1640 complemented with 10% IgG-free Fetal Calf Serum (Sigma-Aldrich, #F1283) into roller bottles (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... The major components of the assay included: 1) rS-RBD coating of plates as target antigen (2ug/ml in Sigma Carbonate-Bicarbonate Buffer); 2 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... cells producing HCMV specific antigens were detected 24h post infection by immunoperoxidase staining using monoclonal antibodies directed against the UL123-coded 72 kDa immediate early antigen 1 (IEA1) (Mouse Anti CMV IEA, MAB8131, Millipore, Temecula, CA, USA) and 120h post-infection by immunoperoxidase staining using monoclonal antibodies directed against UL55-encoded late antigen gB (LA ...
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bioRxiv - Neuroscience 2021Quote: ... subjected to heat induced antigen retrieval in a citrate buffer and incubated with a mouse anti-NeuN antibody (clone A60, Millipore, MAB377, 1:100). The used secondary antibodies were mouse linker antibody (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... antigen retrieval was conducted on deparaffinised 3D epidermal skin equivalents by incubation at 95°C in citrate buffer (Sigma-Aldrich, St. Louis, USA) for 20 mins ...
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bioRxiv - Neuroscience 2021Quote: AIA was induced in male and female mice on a C57BL/6 background using the antigen methylated bovine serum albumin (mBSA, Sigma, St Louis, MO) as described previously26 ...
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bioRxiv - Immunology 2020Quote: ... were done in Balb/c mice (n=4 in each group) through intraperitoneal (ip) injections of 20 μg of antigen in 200 μL of 50% v/v of adjuvant (Sigma Adjuvant System®). For experiments in Fig 4A ...
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bioRxiv - Immunology 2020Quote: ... of 6-8 weeks old were separated into cohorts (10-12 mice) based on antigen: hen egg lysozyme (HEL, Sigma Aldrich, 62971-F), ovalbumin (OVA ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μM-sections were deparaffinized and antigen retrieval was performed in 10 mM citrate buffer (pH = 6; Sigma-Aldrich, St Louis, MO, USA) for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... were deparaffinized and rehydrated and subjected to antigen retrieval using citrate buffer (0.0126 M citric acid, Sigma cat. C-0759; 0.0874 M sodium citrate, Sigma, cat. S-4641; pH 6.0) for a total of 20 minutes ...
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bioRxiv - Physiology 2023Quote: ... MEFs were immortalized by addition of SV40 T antigen lentivirus (Alstem #C1LV01) in the presence of 0.02 mg/mL polybrene (EMD Millipore # TR-1003-G), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatants were screened using ELISA (see below) and antigen-reactive clones were expanded in serum IgG free RPMI-1640 (Sigma-Aldrich, MO, USA) into roller bottles (Sigma-Aldrich ...
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bioRxiv - Genomics 2021Quote: ... Cells were permeabilized by 0.1% Triton X-100 for 5min at RT and stained with antibody against the mitosis specific antigen pMPM2 (Millipore, 05-368, 0.2µl/10million cells) for 50min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... mouse and human brain slices of 20 μm thickness were subjected to antigen retrieval in 10 mM citrate (Sigma-Aldrich, St. Louis, MO, USA) + 0.5% Tween-20 (Merck ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The diluted antigen (100μl) was coated to the wells of a Nunc-Immuno™ MicroWell™ 96 well solid plates (Sigma-Aldrich, MO, USA) and was incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... All mice were immunized 4 times.The administered antigens (sF/sG) were formulated in a Sigma adjuvant system (Sigma-Aldrich Co. LLC, St. Louis, MO). Mouse splenic lymphocytes were isolated 4 days following a final immunization without adjuvant and fused with SP2/0 cells using polyethylene glycol by standard methods ...
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bioRxiv - Neuroscience 2023Quote: ... Mice were immunized on day 0 by subcutaneous injections in the flank region with a total 200 μl of antigen PLP139-151 peptide (100 μg/mouse) emulsified in Complete Freund’s Adjuvant CFA (Sigma Chemicals, St. Louis, MO, USA) supplemented with 4 mg/ml heat-killed Mycobacterium tuberculosis H37Ra (400 μg ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunohistochemical staining was performed on a Leica Bond III instrument using the following primary antibodies and Leica antigen retrieval conditions: involucrin (Sigma-Aldrich, catalog #I9018, 1:10,000), retrieval H1 (30min) ...
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bioRxiv - Plant Biology 2019Quote: ... The antigen–antibody complexes were visualized using nitrotetrazolium blue chloride/5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) buffer (Sigma-Aldrich, St. Louis, Missouri, USA) under standard conditions ...
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bioRxiv - Immunology 2023Quote: ... immunizations were prepared by mixing 10 μg of antigen and 50 μg of alum in 100 μl of sterile TBS (Sigma-Aldrich Alhydrogel, catalog no. T5912) per mouse unless otherwise specified ...
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bioRxiv - Pathology 2022Quote: ... IHC labeling for MMP13 (Thermo Fischer Scientific, Waltham, MA. Catalog #MS-825P) began with enzymatic antigen retrieval using hyaluronidase (Sigma-Aldrich, St. Louis, MO; H-3506) for 10 minutes in a 37°C water bath ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 anti-RBD and N IgG antibody levels were determined using the Milliplex® SARS-CoV-2 antigen panel 1 IgG assay (Millipore Sigma, Cat No. HC19SERG1-85K) with the Wuhan-1 strain RBD and N proteins included ...