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Citations for Millipore Sigma :
501 - 550 of 10000+ citations for Breast Cancer Type 1 Susceptibility Protein BRCA1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: ... 0.2mg of PNS were incubated for 4 hours at 4°C with 5 μl of protein A magnetic beads (Pierce Thermoscientific) pre-incubated (1 hour at RT) with 2 μg of anti-FLAG antibody (Sigma, F7425). Immune complexes were finally washed 3 times with lysis buffer and eluted by incubating the beads (5’ at 70°C ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h at RT with anti-microtubule associated protein 2 (MAP2) mouse monoclonal antibody (diluted 1:200 in PBS/gelatin; Sigma-Aldrich) and with anti-Tau rabbit polyclonal antibody (diluted 1:500 in PBS/gelatin ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2020Quote: ... FLAG- and HA-tagged proteins were detected by Western blotting using a 1:1000 dilution of primary anti-FLAG rabbit polyclonal antibody (Sigma, F7425) or anti-HA rabbit polyclonal antibody (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were next labeled as follows: HA-tagged proteins were detected by anti-HA rat monoclonal antibody 3F10 (Sigma, 1:200), parasite cyst wall and cyst matrix by in-house SalmonE anti-CST1 antibody (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein loading was measured using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000; AC-15, Sigma-Aldrich), nucleolin (1:5000 ...
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bioRxiv - Physiology 2022Quote: ... Membranes was incubated with the primary antibodies against target proteins: rabbit polyclonal anti-TSG 101 (T5701, Sigma-Aldrich Co., 1:200), rabbit polyclonal anti-CD63 (SAB4301607 ...
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bioRxiv - Microbiology 2022Quote: ... Epitope-tagged 3xFLAG proteins were detected using primary antibody anti-FLAG M2 from mouse (diluted 1:4,000, catalogue number F3165; Sigma Aldrich, Germany) and Alexa Fluor 680 fluorescent dye-labeled secondary anti-mouse antibody from goat (diluted 1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... The proteins were transferred onto nitrocellulose membrane using iBlot2 Gel Transfer Device and immunostained using Neurexin-1 antibody (Millipore ABN161-I) and Tuj1 (Biolegend 801201 ...
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bioRxiv - Pathology 2020Quote: ... and specific proteins were detected by immunostaining with the following antibodies: Cy3-coupled mouse anti-ACTA2 (C6198-100UL, 1:500, Sigma-Aldrich) or biotinylated mouse anti-ACTA2 (MS-113-B0 ...
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bioRxiv - Molecular Biology 2020Quote: ... Unbound protein was washed off in PBST (3x 5 min) and bound protein was detected using HRP-conjugated anti-His antibody (1:2000 in 5% milk PBST; Novagen #71841). After 1 hour incubation at room temperature ...
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bioRxiv - Genetics 2022Quote: ... TAP-tagged Rrp45 protein was detected with peroxidase anti-peroxidase (PAP) soluble complex antibody produced in rabbit (1:5000, Sigma-Aldrich). The 3-phosphoglycerate kinase (Pgk1 ...
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bioRxiv - Plant Biology 2022Quote: ... The membranes were washed with Tris buffered saline containing 0.1% Tween-20 and probed again for protein loading control using a 1:10000 dilution of mouse monoclonal anti-actin (plant) primary antibody (Sigma, A0480) and a 1:3000 dilution of goat anti-mouse IgG HRP conjugate (Biorad).
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bioRxiv - Microbiology 2022Quote: ... diluted 1:5000 to detect RVFV and anti-Gc protein antibody (4C12A1) diluted 1:5000 to detect LACV with FFA staining buffer (1X PBS, 1mg/ml saponin (Sigma: 47036)) as a primary detection antibody overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... The sections were then incubated overnight with primary antibodies directed against SARS Nucleocapsid protein (1/500; mouse monoclonal; clone 1C7C7; Sigma-Aldrich), ionised calcium-binding adapter molecule 1 (Iba1 ...
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bioRxiv - Developmental Biology 2022Quote: ... Specific protein detection was performed using diluted primary antibodies as follows: anti-NDRG1 @ 1:5000 (Sigma-Aldrich, catalog# HPA006881, rabbit polyclonal); anti-GAPDH @1:5000 (GeneTex ...
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bioRxiv - Microbiology 2023Quote: ... The upper part was used for protein detection using a specific monoclonal antibody against Cas9 (1:1000 dilution, #MAC133, Sigma-Aldrich) and Alexa555 secondary antibody for fluorescence detection (iBright ...
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bioRxiv - Genomics 2023Quote: ... The following primary antibodies are used for the first target protein at the dilutions indicated: NeuN (1:500, A60, Mouse, Millipore MAB5374), pTDP43 (1:1000 ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were then incubated overnight with primary antibodies against Yes-associated protein (YAP) (mouse monoclonal anti-YAP, 1:400, Sigma-Aldrich) at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein loading was determined using a monoclonal β-actin antibody directly conjugated to horseradish peroxidase (1:20,000) from Sigma-Aldrich (A3854). Quantitation of western blot band intensity was done using Image J software.
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bioRxiv - Neuroscience 2023Quote: ... and the indicated proteins were detected and quantified by immunoblotting with the following antibodies: rat anti-DAT (MAB369, Millipore; 1:2000), rabbit anti-TH (AB152 ...
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bioRxiv - Biochemistry 2024Quote: ... PEGylated full-length proteins were transferred to nitrocellulose membrane and probed with mouse anti-HA antibodies (Sigma-Aldrich, H9658, 1:5000) overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: A2780-DNA329 and OVCAR-3 (ATCC) ovarian cancer cell lines were cultured in RPMI-1640 medium (R8758, Sigma). Ovarian cancer COV362 (ECACC) ...
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bioRxiv - Cancer Biology 2020Quote: ... Human colon cancer cell lines HCT116 TP53+/+ and HCT116 TP53-/- were grown in McCoy’s 5A Medium (Sigma, M9309) supplemented with 10% FBS and Pen/Strep ...
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bioRxiv - Cancer Biology 2024Quote: ... Colorectal cancer cells lines were maintained in high glucose DMEM supplemented with 10% FBS (Cat# F0926, Sigma-Aldrich) 1% antibiotic/antimycotic (Cat# 400-101 ...
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bioRxiv - Cancer Biology 2024Quote: ... The cancer cells were maintained in Roswell Park Memorial Institute (RPMI) medium (Sigma-Aldrich, St. Louis, MO, USA), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... Bacteria were recovered by treating with 300 μl (400 µl wounds) or 150 µl (200 µl wounds) of 0.5 mg·ml-1 collagenase type 1 (EMD Millipore Corp, USA) for 1 hour at 37°C to break down the matrix ...
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bioRxiv - Plant Biology 2019Quote: ... 2004) containing 1% (w/v) sucrose and solidified with 1% (w/v) EDTA-washed Agar Type M (Sigma-Aldrich, Steinheim, Germany), followed by stratification in the dark at 4 °C for 2 d ...
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bioRxiv - Bioengineering 2022Quote: ... A stock solution of 3 mg/mL rat tail collagen type I was neutralized with 1% 1 M NaOH (Sigma, S5761) and 10% 10x phosphate buffered saline ...
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bioRxiv - Microbiology 2023Quote: ... the collagen in the wounds was digested to release bacteria by adding 300 μl 0.5 mg·ml−1 collagenase type 1 (EMD Millipore Corp, USA) and incubating for 1 h at 37°C ...
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bioRxiv - Bioengineering 2022Quote: ... anti-Fatty acid binding protein 1 (FABP1) (1:50, Sigma-Aldrich) and anti-cytokeratin 20 (CK20 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 0.7 mM Na2 HPO4, 10 mM HEPES 5.1 mM CaCl2, pH7.65, type I collagenase from Clostridium histolyticum type IV; Sigma), and homogenate filtrated through a 70 μm cell strainer ...
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bioRxiv - Genomics 2019Quote: ... RNA-protein complexes were incubated with antibody-bound Protein A Dynabeads (anti-FLAG [Sigma Aldrich F3165] or anti-IgG) at 4C for 2 hours ...
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bioRxiv - Plant Biology 2021Quote: ... and immunoprecipitation of the antibody/protein complexes was performed by adding 50 μl of Protein A-Sepharose (Sigma-Aldrich) made up as a 50% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies (PSD95 1:500, Antibodies Inc., Davis CA and MAP2 1:1000; Millipore) were diluted in 1x PBS with 1% BSA and 0.2% Tween ...
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bioRxiv - Physiology 2019Quote: ... Pax7 primary antibody (1:100, DSHB) and laminin primary antibody (1:100, Sigma-Aldrich) were diluted in 1% TSA blocking buffer and applied overnight ...
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bioRxiv - Cell Biology 2020Quote: ... using 6% concanavalin A-Type IV (Sigma). Live-cell imaging were performed using a DeltaVision RT wide-field fluorescence imaging system (Applied Precision ...
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bioRxiv - Developmental Biology 2021Quote: ... Both wild type ATDC5 cells (Sigma, 99072806) and Adgrg6 KO ATDC5 cells were maintained in DMEM/F-12 (1:1 ...
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bioRxiv - Biochemistry 2019Quote: ... 200 of µM wild-type Ub (Sigma) and 10 µM of untagged αS or tauK18 substrate ...
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bioRxiv - Bioengineering 2021Quote: ... type A porcine skin gelatin (Sigma-Aldrich) was mixed at 10% (w/v ...
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bioRxiv - Bioengineering 2021Quote: ... of gastric mucin (Type III, Sigma Aldrich), and 0.05 % wt ...
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bioRxiv - Bioengineering 2020Quote: ... Type A (Bloom strength 300, Sigma-Aldrich) was dissolved at 10% (w/v ...
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bioRxiv - Genomics 2021Quote: ... Type-VS Millipore membrane (Merck Millipore, #VSWP01300). 20 μL ElectroMAX DH10B competent cells (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: ... rat tail collagen type I (Sigma-Aldrich) was dissolved in 0.25% acetic acid and diluted 1:1 with 2 x MEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... 1mg/mL Type IV Collagenase (Sigma-Aldrich), 150U/mL Type IV DNase I (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... and Type IV (from Clostridium, Sigma-Aldrich) in 5 ml of sterile-filtered Dulbecco’s Modified Eagle’s Medium (DMEM3 ...
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bioRxiv - Immunology 2022Quote: ... containing 0.1% type XIV protease (Sigma-Aldrich), 50 U/mL penicillin ...
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bioRxiv - Microbiology 2022Quote: ... Type V collagen from human placenta (Sigma Type IX ...
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Notch signaling functions in non-canonical juxtacrine manner in platelets to amplify thrombogenicitybioRxiv - Cell Biology 2022Quote: ... Type I deionized water (18.2 MΩ.cm, Millipore) was used for preparation of solutions ...